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gene transfer approach
相关语句
  基因转移方法
     Gene transfer is the key point in plant gene engineering research, which covers choosing plant accepter and exploring gene transfer approach.
     植物基因工程研究的关键问题是向植物中转移外源基因,它主要涉及植物受体的选择和基因转移方法的探索。
短句来源
     Although the feasibility of the NOS gene transfer approach has been demonstrated in animal models, currently available vectors have a number of technical and safety limitations that have to be solved before human NOS gene therapy for cardiovascular diseases can be attempted.
     NOS基因转移方法在动物模型中具有可行性 ,但是 ,当前可用的载体还有许多技术和安全限制。 在用于人类心血管疾病NOS基因治疗前必须给予克服
短句来源
  “gene transfer approach”译为未确定词的双语例句
     Sperm-mediated gene transfer approach transfer foreign gene to embryos through fertilization.
     精子载体法介导基因转移是以精子为载体,在受精时将外源目的基因导入卵母细胞,制备转基因动物的简单而高效的方法之一。
短句来源
     To investigate the function of immediately early gene 1 and 2(IE1 and IE2) of human cytomegalovirus on p53 gene in Jurkat cell line that lacks p53 protein expression, gene transfer approach and flow cytometry were used.
     选择野生型P5 3缺失型的Jurkat细胞系 ,首先将wt p5 3 pLXSN稳定转染入Jurkat细胞 (Jurkat wt p5 3) ,再分别或联合转染IE1、IE2基因 ,并利用流式细胞仪分析。
短句来源
     Objective:To examine the expression kinetics and bioactivity of expressed product of mGMCSF gene transfected into B16F1 cells with cationic lipid as a vector in order to explore the potential application of this gene transfer approach.
     目的 :探讨基因转移载体脂质体介导转染的鼠粒细胞 巨噬细胞集落刺激因子 (mGM CSF)基因在B16F1细胞的表达动态及表达产物的生物活性。
短句来源
  相似匹配句对
     Horizontal Gene Transfer
     水平基因转移
短句来源
     Transfer Gene Animals
     论转基因动物
短句来源
     A NEW APPROACH FOR GENE TRANSFER INTO CITRUS
     柑桔基因转化新方法的研究
短句来源
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  gene transfer approach
In this study, we use an efficient adenoviral-based gene transfer approach to introduce a transcriptionally active form of p73 (TA-p73β) in melanoma cells, with the objective of overcoming drug resistance.
      
This study demonstrates the feasibility of expressing recombinant genes (Bax) in anin vivo liposome-mediated gene transfer approach.
      
We have used a cell-based gene transfer approach to define the role of MMPs in a rat xenograft model of human aortic aneurysm.
      
We concluded that the IL-10 adenoviral gene transfer approach allowed us to induce high lung expression of biologically active IL-10 in the lung.
      
We report our further investigations of the amplitude, duration and physiological relevance of this novel gene transfer approach.
      
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The study of plant gene engineering is closely related to the human life. It will bring a revolutionary change to the plant breeding which will contribute a great deal to the people. Gene transfer is the key point in plant gene engineering research, which covers choosing plant accepter and exploring gene transfer approach. With the development and the usage of the Ti-plasmid vector,great progress in applied research has been made in dicots. As to the monocots.it is still in basic research. The present...

The study of plant gene engineering is closely related to the human life. It will bring a revolutionary change to the plant breeding which will contribute a great deal to the people. Gene transfer is the key point in plant gene engineering research, which covers choosing plant accepter and exploring gene transfer approach. With the development and the usage of the Ti-plasmid vector,great progress in applied research has been made in dicots. As to the monocots.it is still in basic research. The present results show that the future is very hopeful.

植物基因工程与人类生活密切相关,它将给植物育种带来革命性的变革。植物基因工程研究的关键问题是向植物中转移外源基因,它主要涉及植物受体的选择和基因转移方法的探索。由于Ti质粒载体的建立和应用,双子叶植物中的应用性研究已经取得了突破性的进展。单子叶植物方面的工作尚处于基础理论研究阶段。

Gene therapy involves the transfer of a functional gene into host cells to correct the malfunction of a specific gene or to alleviate the symptoms of a disease. For gene transfer to the cardiovascular system, adenoviral vectors are the most efficient means of transfer. We reviewed the methods of current progress in the field of vascular NOS gene transfer and the potential use of NOS gene therapy for important cardiovascular diseases. Although the feasibility of the NOS gene transfer approach has been demonstrated...

Gene therapy involves the transfer of a functional gene into host cells to correct the malfunction of a specific gene or to alleviate the symptoms of a disease. For gene transfer to the cardiovascular system, adenoviral vectors are the most efficient means of transfer. We reviewed the methods of current progress in the field of vascular NOS gene transfer and the potential use of NOS gene therapy for important cardiovascular diseases. Although the feasibility of the NOS gene transfer approach has been demonstrated in animal models, currently available vectors have a number of technical and safety limitations that have to be solved before human NOS gene therapy for cardiovascular diseases can be attempted.

基因治疗包括功能基因转移至宿主细胞 ,用于校正特定基因的功能缺陷或减轻疾病的症状。对于心血管疾病的基因转移 ,腺病毒载体最有效。该文从方法学上回顾了血管NOS基因转移领域的最新进展以及NOS基因转移对重要心血管疾病的潜在应用。NOS基因转移方法在动物模型中具有可行性 ,但是 ,当前可用的载体还有许多技术和安全限制。在用于人类心血管疾病NOS基因治疗前必须给予克服

To investigate the function of immediately early gene 1 and 2(IE1 and IE2) of human cytomegalovirus on p53 gene in Jurkat cell line that lacks p53 protein expression, gene transfer approach and flow cytometry were used. A p53 expressed plasmid named wt p53 pLXSN was first introduced stably into Jurkat cells, and then IE1 and IE2 expressed plasmids were transfected transiently respectively. After 24 hours, apoptosis in the group of Jurkat cells transfected with p53 and IE2 was...

To investigate the function of immediately early gene 1 and 2(IE1 and IE2) of human cytomegalovirus on p53 gene in Jurkat cell line that lacks p53 protein expression, gene transfer approach and flow cytometry were used. A p53 expressed plasmid named wt p53 pLXSN was first introduced stably into Jurkat cells, and then IE1 and IE2 expressed plasmids were transfected transiently respectively. After 24 hours, apoptosis in the group of Jurkat cells transfected with p53 and IE2 was found.The analysis of flow cytometry showed that the cells of G 1 and S phases were reduced, most of cells were arrested in G 2/M phase and sub G peak emerged in this group.The results suggest that the IE2 gene of human cytomegalovirus may mediate the pro apoptotic function of p53 gene in Jurkat cells.

选择野生型P5 3缺失型的Jurkat细胞系 ,首先将wt p5 3 pLXSN稳定转染入Jurkat细胞 (Jurkat wt p5 3) ,再分别或联合转染IE1、IE2基因 ,并利用流式细胞仪分析。结果显示 ,转染了IE2基因的Jurkat wt p5 3细胞在瞬时转染 2 4h后出现光镜下可见的细胞凋亡改变 ,此时取样进行流式细胞仪测定细胞周期分布状况 ,可见转染了IE2基因的Jurkat wt p5 3细胞周期分布较其他各组发生了明显的变化 ,G1期和S期细胞分布减少 ,而G2 期细胞增多 ,细胞阻滞于G2 /M期 ,并且出现了细胞凋亡亚二倍体峰 ,引起细胞凋亡。研究表明 ,在Jurkat细胞中HCMV的IE2基因可介导野生型p5 3基因的促凋亡作用。

 
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