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glycosylation transfer gene
相关语句
  糖基转移酶基因
     ORF2 shows 54% homology with hupE of Rhizobium leguminosarum,and related with glycosylation transfer gene of Rhizobium meliloti. Names enfA and enfB are proposed to ORF1 and ORF2 respectively.
     ORF2编码含235个氨基酸的多肽,与碗豆根瘤菌的hupE及苜蓿根瘤菌的糖基转移酶基因显示54%的同源性。
短句来源
  相似匹配句对
     TECHNOLOGY TRANSFER
     国内外科技成果转让项目
短句来源
     TECHNOLOGY TRANSFER
     山东省高新技术转移中心项目推荐
短句来源
     Protein Glycosylation Engineering
     蛋白质糖基化工程
短句来源
     Transfer of Mammalian Genes
     哺乳类基因的转移
短句来源
     Horizontal Gene Transfer
     水平基因转移
短句来源
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The cosmid gene library of Rhizobium fredii strain B52 genome was constructed, and a 3. 7kb enhancing fragment was isolated through plant experiment, which could improve the symbiotic efficiency of Bradyrhizobium strain 2210. The obtained highly effective strain was called HN32. It has been widely used as soybean inoculant in Helongjiang, Sichuan and Guangxi provinces with an average of 6% yield increase comparing with 2210. The recombinant plasmid pHN32 in strain HN32 contains a 3. 7kb foreign insert (Zhou...

The cosmid gene library of Rhizobium fredii strain B52 genome was constructed, and a 3. 7kb enhancing fragment was isolated through plant experiment, which could improve the symbiotic efficiency of Bradyrhizobium strain 2210. The obtained highly effective strain was called HN32. It has been widely used as soybean inoculant in Helongjiang, Sichuan and Guangxi provinces with an average of 6% yield increase comparing with 2210. The recombinant plasmid pHN32 in strain HN32 contains a 3. 7kb foreign insert (Zhou et al. 1990). Its nucleotide seuqence was analyzed. Computer analysis indicated that there were two open reading frames (ORFs) within the 3. 7kb enhancing fragment. ORF1 shows no homology with known genes except the first 19 amino acids which had high homology with lactose transferase of Agrobacterium trdiobacter. ORF2 shows 54% homology with hupE of Rhizobium leguminosarum,and related with glycosylation transfer gene of Rhizobium meliloti. Names enfA and enfB are proposed to ORF1 and ORF2 respectively.

利用柯氏质粒pLAFR1为载体构建了快生型大豆根瘤菌B52菌株的基因文库,并通过植物结瘤试验筛选到一个3.7Kb增效片段,将其导入慢生型大豆根瘤菌2210,能提高其共生固氮效率。所得高效菌株HN32在黑龙江、四川和广西大面积应用,平均增产11.4%(5)。本文测定了3.7Kb片段的核苷酸序列,计算机分析发现含有两个开放阅读框架(ORFs)。ORF1编码含190个氨基酸的多肽,与已报道基因和多肽的同源性很低,但其N端19个氨基酸与发根农杆菌的乳糖转移酶高度同源。ORF2编码含235个氨基酸的多肽,与碗豆根瘤菌的hupE及苜蓿根瘤菌的糖基转移酶基因显示54%的同源性。

 
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