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clone and identify
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  克隆和鉴定
     Objective:To clone and identify the genes related to IL 6 in order to reveal mechanisms of IL 6 signal transduction and to discover new signal transduction molecules.
     目的 :克隆和鉴定IL 6作用相关基因将有助于揭示IL 6信号转导机制和发现新的信号分子。
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     Objective To clone and identify MF 1 gene which responded to extremely low frequency magnetic fields(ELF MF) in Daudi cells,and explore the response universality of MF 1 gene in several MF sensitive cell lines,so as to provide experimental basis for revealing the mechanism of biological effects induced by magnetic field.
     目的 克隆和鉴定本研究室经DD法在Daudi细胞中筛选到的一个极低频磁场的特异反应基因 (MF 1) ,并在多种磁场敏感细胞中证实该基因反应的普遍性 ,为揭示磁场所致生物学效应的作用机制提供实验依据。
短句来源
     Objective To clone and identify the differentially expressed genes of rat intestinal epithelial cell line (IEC 6) before and after exposure to high dose radiation so as to provide proof for the investigation of the molecular mechanisms in the repair of radiation damage of intestinal epithelial cells.
     目的 克隆和鉴定大剂量辐射前后大鼠空肠上皮细胞IEC 6差异表达基因 ,为探索肠上皮细胞辐射损伤修复的分子机制提供线索。
短句来源
     Objective To clone and identify ubiguitin-conjugating enzyme E gene of Schistosoma japonicum Chinese strain (SjUCEE).
     目的 日本血吸虫中国大陆株泛蛋白缀合酶 E (ubiquitin- conjugating enzyme E ,Sj UCEE)的基因克隆和鉴定
短句来源
     Objective To clone and identify a novel collagen(COL) cDNA of Angiostrongylus cantonensis.
     目的克隆和鉴定广州管圆线虫(Angiostrongyluscantonensis)新基因——胶原蛋白(collagen,COL)全长cDNA序列。
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  克隆与鉴定
     Using Lab On-line to Clone and Identify the Esophageal Cancer Related Gene 4
     网上实验室克隆与鉴定食管癌相关基因ECRG-4
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     Aim To clone and identify 14-3-3 signal transduction protein gene of Toxoplasma gondii (Toxo14-3-3) for characterization of new candidate vaccine and diagnostic molecules and for search for a metabolic interference with parasite invasion to host cells.
     目的 克隆与鉴定弓形虫 14- 3- 3(Toxo14- 3 - 3)信号转导蛋白基因 ,用于寻找弓形虫新的诊断和候选疫苗分子 ,并在寄生虫入侵宿主细胞中寻找分子干预环节。
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     Objective:To clone and identify BPI 600 bp gene which encode rBPI 23 .Methods:The gene which encode rBPI 23 were amplified by RTPCR from mRNA that were extracted from HL60.Recombinant cloning vector was constructed sequenced after the enzyme digestion.
     目的 :rBPI2 3基因 (BPI60 0bp)克隆与鉴定。 方法 :采用RT PCR技术 ,从HL 6 0细胞mRNA中扩增编码BPIN端 199个氨基酸 (rBPI2 3)的基因片段 (BPI60 0bp) ,经酶切后通过连接反应构建重组克隆载体 ,测序鉴定。
短句来源
     Objective:To clone and identify P58.2 gene which encodes natural killer cell inhibitory receptor.
     目的 :P5 8.2基因克隆与鉴定
短句来源
     Purpose:To stuey the tissue specificity of Chinese uroplakin Ib and clone and identify its promoter.
     目的:研究中国人尿路斑块蛋白Ib的组织特异性并克隆与鉴定其启动子。
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  “clone and identify”译为未确定词的双语例句
     Aim: To clone and identify 14-3-3 protein cDNA fragment from Dunaliella salina.
     目的:克隆杜氏盐藻14-3-3蛋白的cDNA片段,并对其进行测序和序列分析。
短句来源
     Objective To clone and identify cDNA of mouse C3d.
     目的 获得小鼠C3d分子的cDNA。
短句来源
     Although CD209L was recently determined to be another receptor, angiotensin-converting enzyme (ACE2), a carboxypeptidase and an essential regulator of heart function, was the major functional receptor of SARS-CoV. This thesis was aimed to clone and identify ACE2, identify the functional domain of ACE2, and provide evidence to further study the interaction between SARS-CoV and host cells, the interspecies infection of SARS-CoV and development of antiviral agents.
     SARS-CoV的细胞受体已鉴定为血管紧张素转换酶2(angiotensin-converting enzyme2,ACE2)。 尽管CD209L也被认为是SARS-CoV的细胞受体,但血管紧张素转换酶2(ACE2)是SARS-CoV的主要功能性受体。
短句来源
     Aims: To clone and identify the full-length cDNA of IL-10 gene in Sprague-Dawley (SD) rats, so as to pave the way for the construction of adenovirus recombinants carrying rat IL-10 gene and for the study of gene therapy on liver fibrosis.
     目的:克隆并鉴定Sprague鄄Dawley(SD)大鼠IL鄄10基因的全长cDNA,为进一步构建大鼠IL鄄10腺病毒重组子和肝纤维化基因治疗的研究奠定基础。
短句来源
     Clone and Identify Gene cDNA Fragments Related to Gastric Cancer and Its Premalignant Lesion
     胃癌及其癌前病变相关基因cDNA片段的克隆和初步鉴定
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  clone and identify
chrysogenum, using a suppression subtractive hybridization approach to clone and identify sequences of genes differentially expressed in media with glucose or lactose as carbon source (penicillin-repressing or non-repressing conditions).
      
Experiments were designed to clone and identify genes of the fungal phytopathogen Colletotrichum gloeosporioides expressed at high levels during growth on the compatible host Stylosanthes guianensis when compared with expression in axenic culture.
      
These data will facilitate attempts to clone and identify more shrimp genes and constitutive shrimp promoters.
      
Our goal was to clone and identify new cancer-predisposing genes.
      


Objective To clone and identify the whole cDNA of MXR7 gene and to fied out its exprmsion in human normal and tumor tissues. Method The DNA primers were designed and syntheised acordingto the whole cDNA sequence of MXR7 gene. The cDNA of human HCC was taken as template while the cDNA of MXR7 gene was synthesized by polymerase chain reaction (PCR), Recombinant DNA conforming toreading frame was constructed by connecting purified PCR product of the cDNA of MXR7 gerre with expression vector pGEX-5X-1...

Objective To clone and identify the whole cDNA of MXR7 gene and to fied out its exprmsion in human normal and tumor tissues. Method The DNA primers were designed and syntheised acordingto the whole cDNA sequence of MXR7 gene. The cDNA of human HCC was taken as template while the cDNA of MXR7 gene was synthesized by polymerase chain reaction (PCR), Recombinant DNA conforming toreading frame was constructed by connecting purified PCR product of the cDNA of MXR7 gerre with expression vector pGEX-5X-1 of fusion protein. The plasmid MXR7/pGEX-5X-1 was identified by sequencing. Us ing 32 P labeled MXR7cDNA as probe, MXR7mRNA expression was detected by Northern blot analysis in 12different human normal tissues, 7 preoperatively untreated non-liver tumor tissue, 30 preoperatively UntreatedHCC and the corresponding surrounding non-cancerous hepatic tissues samples, 12 normal liver tissues samplesconfirmed pathologically. Results Enzyme digest and sequence analysis confirmed that the insertion sequencein vector PGEX-5X-1 was the same as the cDNA sequence of MXR7 gne. Northern blot analysis showed nodetectable expression of MXR7 mRNA in 12 human normal tissues including liver, 7 non-HCC tumor tissuesand 12 normal liver tissues and the frequencies of MXR7 mRNA expression in HCC and the coresponding sur rounding non-cancerous hepatic tissues are 76.6% and 13.3%, respectivdy. Concluslon MXR7 mRNAhigh expression in human HCC was common and specific, suggesting MXR7 mRNA may serve as a tumorbiomarker for HCC.

进行MXR7基因cDNA的克隆鉴定并检测其在人正常组织和肿瘤组织中的表达。方法以人肝癌组织的cDNA为模板,应用PCR方法合成MXR7基因cDNA,连接于融合蛋白表达载体pGEX-5X-1,构建成符合读框的融合基因;应用Northernblot对正常人12种不同组织和术前做治疗的7例非肝脏肿瘤组织及30例肝癌和癌旁肝组织、12例正常肝组织中MXR7基因的表达进行检测。结果MXR7mRNA在人肝脏等12种正常组织及7例其它部位肿瘤组织中均不表达;在人肝癌中呈高表达,而癌旁肝组织中呈低表达,其阳性表达率分别为76.7%和13.3%。结论MXR7.基因在人肝癌组织中的高水平表达具有普遍性和特异性,提示MXR7基因可以作为肝癌的生物学标志物。

Objective[WT5”BZ] To clone and identify RCC specially expressed genes different with normal kidney tissue. [WT5”HZ]Methods[WT5”BZ] A technique called suppression subtractive hybridization was used to construct the library which contains the differently expressing cDNAs between RCC and normal kidney. Then the RCC specially expressed genes were cloned from it. [WT5”HZ]Results[WT5”BZ] Human RCC subtractive library with high subtractive efficiency was set up successfully. The amplified library contains...

Objective[WT5”BZ] To clone and identify RCC specially expressed genes different with normal kidney tissue. [WT5”HZ]Methods[WT5”BZ] A technique called suppression subtractive hybridization was used to construct the library which contains the differently expressing cDNAs between RCC and normal kidney. Then the RCC specially expressed genes were cloned from it. [WT5”HZ]Results[WT5”BZ] Human RCC subtractive library with high subtractive efficiency was set up successfully. The amplified library contains 350 positive clones.Sequence analysis was performed in 5 clones. All of the sequences were unknown before and the cDNA inserting GYLZ RCC18 had three copies. Northern blot analysis showed that GYLZ RCC18 cDNA expressed highly in RCC, but there was no any signal could be detected in normal kidney.Using SMART RACE technique,we obtained the full length of novel gene of GYLZ RCC18.We also identified that GYLZ RCC18 family contains 3 subtype genes. [WT5”HZ]Conclusions[WT5”BZ] The highly efficient cDNA subtractive library lays a solid foundation for screening and cloning new and specific oncogenes or tumor suppressor genes of RCC.The novel differentially expressed genes provide an important clue to studying the mechanism of the occurrence and develpment of RCC. [WT5”HZ]

目的 克隆并鉴定肾癌与正常肾组织之间差异表达的基因 ,为研究肾癌发生发展机制提供新的突破口。 方法 应用抑制性消减杂交技术 ,构建人肾癌组织与正常肾组织差异表达的cDNA消减文库 ,并从中克隆鉴定出肾癌特异表达的基因。结果 :构建成功高消减效率的人肾癌组织cDNA消减文库 ,对其中 10个克隆的插入cDNA片段进行测序后经基因库检索表明 10个片段均为未知新序列 ,其中GYLZ RCC18基因为 5个拷贝 ,这提示以上 10个cDNA片段可能来自 6个新基因。差异分析显示GYLZ RCC18在肾癌组织中有明显表达 ,而在正常肾组织中无表达。应用SMARTRACE技术获得GYLZ RCC18基因的全长 ,并证明GYLZ RCC18是一个 5′端有D3种不同剪切方式亚型的基因家族。 结论 GYLZ RCC18基因是肾癌特异表达的新基因。人肾癌cDNA消减文库可作为筛选、克隆肾癌特异性表达的未知新基因的基础。

Objective:To clone and identify BPI 600 bp gene which encode rBPI 23 .Methods:The gene which encode rBPI 23 were amplified by RTPCR from mRNA that were extracted from HL60.Recombinant cloning vector was constructed sequenced after the enzyme digestion.Results:①Prospective amplified productsBPI 600 bp gene was obtained by RTPCR method.②PUC18BPI 180 、PUC18BPI 420 cloning vector were successfully constructed,and the enzyme digestion analysis are identical with prospective results.③Sequences...

Objective:To clone and identify BPI 600 bp gene which encode rBPI 23 .Methods:The gene which encode rBPI 23 were amplified by RTPCR from mRNA that were extracted from HL60.Recombinant cloning vector was constructed sequenced after the enzyme digestion.Results:①Prospective amplified productsBPI 600 bp gene was obtained by RTPCR method.②PUC18BPI 180 、PUC18BPI 420 cloning vector were successfully constructed,and the enzyme digestion analysis are identical with prospective results.③Sequences are identical with those of the report.Conclusion:PUC18BPI 180 、PUC18BPI 420 cloning vector were successfully constructed,and BPI 600 bp gene sequences are identical with those of the report.

目的 :rBPI2 3基因 (BPI60 0bp)克隆与鉴定。方法 :采用RT PCR技术 ,从HL 6 0细胞mRNA中扩增编码BPIN端 199个氨基酸 (rBPI2 3)的基因片段 (BPI60 0bp) ,经酶切后通过连接反应构建重组克隆载体 ,测序鉴定。结果 :RT PCR获得预期的扩增产物—BPI60 0bp基因片段。成功构建PUC18 BPI180 、PUC18 BPI4 2 0 重组克隆载体 ,酶切、酶谱分析与预期结果相符。DNA测序结果与文献报道一致。结论 :PUC18 BPI180 、PUC18 BPI4 2 0 重组克隆载体构建成功 ;BPI60 0bp基因序列与文献报道一致

 
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