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osteogenesis differentiation
相关语句
  成骨分化
     Osteogenesis differentiation of MSCs regulated by BMPs signal passageway
     BMPs信号通路调控骨髓间质干细胞的成骨分化
短句来源
     Study on Cultured rMSCs Proliferation and Osteogenesis Differentiation Affected by Titanium Particles Loading
     钛颗粒负荷对骨髓间质干细胞增殖及成骨分化的影响
短句来源
     METHODS:Osteogenesis differentiation characteristic of BMSC were compared in three different conditions: ordinary culture medium (DMEM/F12), induced medium and co culture with fresh bone particles. It was to observe the form of cells, alkaline phosphatase (ALP) activity and osteocalcin level. Meanwhile, collagen Ⅰand osteocalcin were assayed with immunocytochemical method.
     方法:比较骨髓间充质细胞在普通培养基培养、诱导培养基培养和新鲜兔骨碎粒的共培养3种条件下的成骨分化特性,观察3种条件下细胞的形态及碱性磷酸酶(ALP)活性、骨钙素水平,并用免疫组化方法检测Ⅰ型胶原、骨钙素。
短句来源
  “osteogenesis differentiation”译为未确定词的双语例句
     Isolation, Culture, Adipogenisis and Osteogenesis Differentiation of Rabbit Bone Marrow Mesenchymal Stem Cells in Vitro
     兔BMSCs体外培养及定向诱导为脂肪细胞、成骨细胞的研究
短句来源
     If the frequency of PEMFs changed,the effects of PEMFs on accelerating hMSCs proliferation and osteogenesis differentiation would changed or not?
     然而刺激的频率不同,是否会对细胞增殖及分化产生不同的影响?
短句来源
     Previous studies indicated that mechanical stretch can induce osteogenesis differentiation of human periodontal ligament cells( hPDLCs).
     机械力对牙周改建作用影响较大,机械力对于牙周膜细胞生物学特性的影响近年来受到学者的广泛关注,以往研究表明牙周膜细胞具有成骨样细胞表型特征,在机械力作用下可以向成骨细胞或成牙骨质细胞分化。
短句来源
  相似匹配句对
     Osteogenesis differentiation of MSCs regulated by BMPs signal passageway
     BMPs信号通路调控骨髓间质干细胞的成骨分化
短句来源
     Differentiation and Analysis
     辨与析
短句来源
     On Automatic Differentiation
     自动微分的基本思想与实现
短句来源
     Study on Cultured rMSCs Proliferation and Osteogenesis Differentiation Affected by Titanium Particles Loading
     钛颗粒负荷对骨髓间质干细胞增殖及成骨分化的影响
短句来源
     The diagnosis of osteogenesis imperfecta
     成骨不全的X线诊断
短句来源
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AIM:To investigate osteogenic characteristics of rabbit bone marrow mesenchymal stem cells (BMSC) under different culture conditions. METHODS:Osteogenesis differentiation characteristic of BMSC were compared in three different conditions: ordinary culture medium (DMEM/F12), induced medium and co culture with fresh bone particles. It was to observe the form of cells, alkaline phosphatase (ALP) activity and osteocalcin level. Meanwhile, collagen Ⅰand osteocalcin were assayed with immunocytochemical method....

AIM:To investigate osteogenic characteristics of rabbit bone marrow mesenchymal stem cells (BMSC) under different culture conditions. METHODS:Osteogenesis differentiation characteristic of BMSC were compared in three different conditions: ordinary culture medium (DMEM/F12), induced medium and co culture with fresh bone particles. It was to observe the form of cells, alkaline phosphatase (ALP) activity and osteocalcin level. Meanwhile, collagen Ⅰand osteocalcin were assayed with immunocytochemical method. RESULTS:Corresponding ALP activity and osteocalcin level of mesenchymal cells, which were co cultured and induced cultured, were significantly higher than that in ordinary cultured group (P< 0.01), with induction cultured the highest; immunohistochemistry of collagen Ⅰand osteocalcin was positive with induced culture and co culture; while negative in control group. CONCLUSION:Induced culture and co cultured can promote the transformation of BMSC into osteoblasts, and make ALP activity and osteocalcin to a high level through a short period of induced culture.

目的:观察不同培养的条件下兔骨髓间充质细胞的成骨特性。方法:比较骨髓间充质细胞在普通培养基培养、诱导培养基培养和新鲜兔骨碎粒的共培养3种条件下的成骨分化特性,观察3种条件下细胞的形态及碱性磷酸酶(ALP)活性、骨钙素水平,并用免疫组化方法检测Ⅰ型胶原、骨钙素。结果:共培养及诱导培养的间充质细胞同期ALP活性及细胞内骨钙素均显著高于普通培养组(P<0.01),诱导培养最高;经过诱导培养及共培养的间充质细胞,其Ⅰ型胶原、骨钙素免疫组化阳性;对照组Ⅰ型胶原免疫组化弱阳性、骨钙素免疫组化阴性。结论:诱导培养及共培养条件呈现促间充质细胞向成骨方向转化的特点,诱导培养短期能使ALP、骨钙素达到较高水平。

Aim: To explore the effect of Hepes on the purification and cultivation of human mesenchymal stem cells(hMSC).Methods: By using holo-marrow direct inoculation, the fresh femoral bone marrow of 13~18 weeks age embryo was cultured. When the adherence cells were confluenced to 90%, the cells were digested and subcultured. The passage cells were inoculated in conventional L-DMEM media and in improved media that was L-DMEM pretreated with Hepes at 15 mmol/L, respectively. The growth of cells was observed. The pH...

Aim: To explore the effect of Hepes on the purification and cultivation of human mesenchymal stem cells(hMSC).Methods: By using holo-marrow direct inoculation, the fresh femoral bone marrow of 13~18 weeks age embryo was cultured. When the adherence cells were confluenced to 90%, the cells were digested and subcultured. The passage cells were inoculated in conventional L-DMEM media and in improved media that was L-DMEM pretreated with Hepes at 15 mmol/L, respectively. The growth of cells was observed. The pH value was measured. The purity of cells was analyzed by using flow cytometry. The cultural MSC were induced to differentiate into osteoblast.By using enzyme cytochemistry and automatic biochemistry analyzer, the alkaline phosphatase activities were detected, and the osteogenesis differentiation capability of MSC was determined.Results: hMSC cultured in 2 mediums had resembling cell configuration,growth characteristics and surface antigen presentation. But in the improvement media, the purity coefficient and multiplication speed of MSC were elevated. Conclusion: Hepes can increase the purity coefficient and multiplication speed of hMSC, reduce the separating procedures of bone marrow cells, and decrease cell damage.

目的:探讨羟乙基呱嗪乙硫磺酸对人骨髓间充质干细胞(hMSC)纯化培养的影响。方法:采用全髓直接接种法分离培养13~18周胎龄水囊引产新鲜胎儿股骨骨髓,贴壁细胞达90%以上融合时消化传代。传代细胞分别接种于含100ml/L胎牛血清的LDMEM培养基(常规培养基)和加入15mmol/L羟乙基呱嗪乙硫磺酸(Hepes)的常规培养基(改良培养基)中,观察细胞生长情况,用酸度计监测培养基pH值变化情况,流式细胞仪鉴定细胞纯度及CD抗原表达情况。并诱导改良培养基培养的hMSC向成骨细胞分化,酶细胞化学法和全自动生化仪检测碱性磷酸酶(ALP)活性,鉴定hMSC的成骨分化能力。结果:2种培养基培养的hMSC在细胞形态、生长特性、CD抗原表达等方面相似,但改良培养基由于pH值较恒定而能提高hMSC纯度及细胞增殖速度,且诱骨分化后可形成矿化结节,ALP染色阳性。结论:Hepes能提高全髓直接接种法培养的hMSC纯度及细胞增殖速度,简化骨髓细胞分离程序,减少细胞损伤及受污染机会,利于hMSC培养的推广应用。

 
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