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   p 53 cdna 的翻译结果: 查询用时:0.009秒
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p cdna
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  p53基因
     Methods A human P 53 cDNA was inserted into a mammalian expression vector P CR 3.1 orientationally after using EcorR ,XbaⅠenzymes digestion to make a P 53 antisense RNA expression vector P CR 3.1 P 53 (AS).
     方法 采用定向克隆法将人野生型P53 基因cDNA反向插入到哺乳动物表达载体PCR3.1的EcorRⅠ和XbaⅠ位点 ,构建了反义PCR3.1 P53 (AS)表达载体 .
短句来源
     Conclusions p53 cDNA mutation is associated with mutant p53 protein overexpression.
     结论 p53基因cDNA突变与突变型p53基因蛋白高表达密切相关。
短句来源
     p53 cDNA MUTATION AND P53 PROTEIN OVEREXPRESSION IN PROGNOSIS OF COLON CANCER
     p53基因cDNA突变及其蛋白高表达与大肠癌预后关系
短句来源
     Methods: The p53 cDNA expression vector pRc/p53 was constructed by inserting a 2 160bp p53 cDNA fragment into digested pRc/CMV vector with T4 ligase by blunt ligation manner. The pRc/p53 plasmid was transfected into K562 cells using lipofectin as mediator. Then, the suppression activity of p53 gene on the colony forming activity in K562 cells was measured with colony culture method in vitro.
     方法:以T4连接酶把2160bp的p53cDNA片段插入pRc/CMV质粒,重组构建pRc/p53表达质粒,以Lipofectin介导pRc/p53质粒转染K562细胞,以甲基纤维素半固体培养方法作K562细胞体外克隆培养,观察转染p53基因后K562细胞克隆形成改变。
短句来源
     The data showed that whereas p53 cDNA mutation was associated with P53 protein overexpression ( P <0.05), and p53 cDNA mutation did provide prognostic information ( P <0.05), while P53 protein overexpression did not.
     p53基因cDNA突变与大肠癌预后相关(P<0.05)。 p53基因cDNA突变与p53基因蛋白高表达直接相关(P<0.05)。
短句来源
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  “p 53 cdna”译为未确定词的双语例句
     Cloning, expression and signification of human wild type p53 cDNA
     人野生型p53 cDNA的克隆、表达和意义
短句来源
     p53 cDNA mutation and mutant p53 protein expression: investigation of their relation with prognosis of colon cancer
     p53 cDNA突变和突变型p53蛋白表达与大肠癌预后的关系
短句来源
     the authors used molecular biology soft Gene Construnction Kit 2.0(GCK2.0) to analyse the process of gene cloning for the construction strategy of pTRE-p53.Compared with the results of the experiments,the authors found the pTRE-p53 plasmid including p53 cDNA sequence was successfully constructed.
     利用分子生物学软件GCK2.0对整个克隆过程进行分析,制定克隆策略,通过测序鉴定结果,得到了含目的基因p53cDNA全序列的pTRE-p53重组质粒。
短句来源
     The Inhibition of Colony Forming Activity in K562 Cells by Transfection of p53 cDNA Plasmid in Vitro
     转染p53抑癌基因体外抑制K562细胞克隆的形成
短句来源
     METHODS:Southern blot by p53 cDNA and pYNZ22 VNTR probes located in 17p13 region were employed to detect the DNA of paired gastric tumor and normal tissues.
     方法:应用位于17p13区域的p53cDNA探针和pYNZ22VNTR探针,对胃癌及癌旁正常组织基因组DNA进行Southern杂交。
短句来源
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  相似匹配句对
     temperature 53℃.
     温度53℃。
     p53mutationisanearlyeventinovariancarcinogenesisandmayserveasageneticmarkerforearlydiagnosisandprognosis.
     p53基因突变为卵巢癌发生过程中的早期事件,可作为早期诊断和判断预后的检测指标之一。
短句来源
     1 53?
     细胞凋亡指数(AI,‰)为7.56±1.53;
短句来源
     53 references are cited.
     文末附参考文献53篇。
短句来源
     Methods The cDNA of g.
     方法合成g.
短句来源
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  p cdna
p53 function can be suppressed by the ectopic expression of p53-derived peptides, isolated earlier using functional selection of genetic suppressor elements (GSEs) from a library of randomly fragmented p53 cDNA (Ossovskaya et al.
      
All these cell lines were transfected with wt-p53 cDNA or the s-myc gene linked to the mouse mammary tumor virus (MMTV) promoter.
      
Flow cytometric studies showed a 65% and 45% enhancement in apoptosis and necrosis of K562 and CCRF-CEM cells transfected with complex of dendrosomes and wild-type p53 cDNA in comparison to controls.
      
Apoptosis Induction in Human Lymphoma and Leukemia Cell Lines by Transfection via Dendrosomes Carrying Wild-Type p53 cDNA
      
Sequence analysis of p53 cDNA revealed a homozygous double mutation at codon 249 (commonly mutated in aflatoxin-associated hepatocellular carcinoma) and codon 250.
      
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The ~(32)P-cDNA Probes of Citrus Exocortis Viroid (CEV) and Chrysanthemum Stunt Viroid (CSV)RNA has been synthesied respectively and usedfor detecting some samples that may contain relative viroids. This is a sensitive and rapid method for diagnosing early viroid disease as compared with biology assays and polyacrylamide gel electrophoresis,

分别用人工合成的柑桔裂皮类病毒和菊花矮化类病毒的互补DNA探针,经固相分子杂交(dot blot)检测了柑桔、菊花等样品。结果表明:与生物学检测和聚丙烯酰胺凝胶电泳(PAGE)分析相比,这是早期诊断类病毒病和检测类病毒的一种较为灵敏、快速的方法。

The gene expression of both the mouse plasmocytoma (SP2/0) and the hybrid cells crossed with rat nucleated erythroblasts were detected by in situ hybridization technique using the probes of mouse β-globin gene and 7 oncogenes (v-Ki-ras, v-H-ras, v-sis, erb-B, v-abl, v-fos, c-myc). After plasmic amplification, DNA was isolated by alkali lysis, purified and recovered, the DNA containing gene fragments were labelled with ~(32)P to become high activity ~(32)P-cDNA probes through nick translation,...

The gene expression of both the mouse plasmocytoma (SP2/0) and the hybrid cells crossed with rat nucleated erythroblasts were detected by in situ hybridization technique using the probes of mouse β-globin gene and 7 oncogenes (v-Ki-ras, v-H-ras, v-sis, erb-B, v-abl, v-fos, c-myc). After plasmic amplification, DNA was isolated by alkali lysis, purified and recovered, the DNA containing gene fragments were labelled with ~(32)P to become high activity ~(32)P-cDNA probes through nick translation, and the labelled probes were used to detect the gene transcripts in cellular level. The results indicated that: (1) no mouse β-globin gene transcripts could be detected in the cytoplasm of SP2/0 cells, as well as in hybrid cells within 72 hours after cell fusion, but transcript signals could be observed in hybrid cells from 4th to 26th passages. (2) Active expression of multioncogenes in SP2/0 cells was demonstrated, all the 7 oneogenes tested, except v-sis, were expressed more strongly. On the other hand, the expression of oncogenes in hybrid cells was found to be dramatically decreased, among them, the oncogenes of c-myc and Ki-ras been suppressed completely. After long term of passages (26th subcultures), the expression of c-myc and Ki-ras was still lower than that of SP2/o ceils although in some cases other oncogenes increased in their expression levels. These results confirmed that the multistep carcinogenesis involved multi-oncogenes expression and that the decancerization of tumor cells may be due to the suppression of multi-oncogenes activity as well as to activate the expression of differentiation genes.

本实验利用小鼠β-珠蛋白基因探针和7种癌基因探针,对细胞的基因表达状态进行探测。含有基因片段的探针经过扩增、DNA碱抽提以及鉴定回收,然后经缺口翻译法制备高比活性的~(32)P标记的cDNA探针,通过原位杂交方法在细胞水平检测基因转录物。结果发现:1.小鼠浆细胞瘤(SP2/0)细胞始终未有小鼠β-珠蛋白基因的转录活动,在它与大鼠中幼、晚幼红细胞杂交后72小时的标本中,也未测到相应的转录产物;但自第4代杂交细胞起,直至第26代杂交细胞,均可测到信号强弱不同的小鼠β-珠蛋白基因产物。2.对SP2/0细胞进行7种癌基因的检测,发现除SiS表达较微弱外,其余6种癌基因均有较强的表达。对杂交细胞各代进行同样的原位杂交检测,可见癌基因的表达均有不同程度的减弱,其中尤以C-myc和Ki-ras癌基因受到抑制的现象最为明显。在较长时间传代(26代)之后,有些杂交细胞几乎又可测到类似SP2/0细胞的癌基因表达程度,但C-myc和Ki-ras的表达水平仍较SP2/0细胞弱。上述结果表明,多阶段肿瘤发生过程涉及多癌基因的活化和表达;而肿瘤细胞去恶性化,可能与相应癌基因表达受抑制以及分化基因的激活和持续表达有关。

The activities of low density lipoprotein (LDL) receptors in 15 organs and tissues from 5 human fetuses of the ages of 22 to 34 weeks were determined using a membrane filter assay of the specific binding of ~(125)I-LDL to tissue homogenates. The results showed that the adrenal cortex (465 ng/mg protein) had the highest activity of ~(125)I LDL specific binding seven times than that of the medulla. Adipose tissue (214 ng/mg protein) and liver (102 ng/mg protein) ranked second. Relatively high bindings were also...

The activities of low density lipoprotein (LDL) receptors in 15 organs and tissues from 5 human fetuses of the ages of 22 to 34 weeks were determined using a membrane filter assay of the specific binding of ~(125)I-LDL to tissue homogenates. The results showed that the adrenal cortex (465 ng/mg protein) had the highest activity of ~(125)I LDL specific binding seven times than that of the medulla. Adipose tissue (214 ng/mg protein) and liver (102 ng/mg protein) ranked second. Relatively high bindings were also observed in the skeletal muscle (89 ng/mg protein), brain (65 ng/mg protein), kidney (60 ng/mg protein) and spinal cord (56ng/mg protein). It was found that the activity of LDL receptors in the central nervous system of human fetus was higher than that of cows, of human adultsand of humam fetuses of 16 to 20 weeks as reported by other investigators. Relatively high level of LDL receptor mRNA in human fetal brain was also found by ~(32)P-cDNA probe hybridization analysis. It is suggested that the growing and developing central nervous system of human fetus reqiresmore cholesterol. In addition, We also observed preliminarily that the tendency of LDL receptors activities in liver and adrenal gland cortex were gradually inereased with fetal age.

本文采用组织匀浆~(125)I-LDL特异性结合膜滤过法,测定了5例22~34周龄人胎儿的15种器官与组织LDL受体的活性。结果表明,肾上腺皮质~(125)I-LDL特异性结合(465ng/mg蛋白)活性最高,为其髓质的7倍多。脂肪组织(214ng/mg蛋白)和肝脏(102ng/mg蛋白)的LDL受体活性其次。结合活性较高的组织还有骨骼肌(89ng/mg蛋白)、脑(65ng/mg蛋白)、肾脏(60ng/mg蛋白)及脊髓(56ng/mg蛋白)等。与其他研究者报道不同的是发现人胎儿中枢神经系统LDL受体活性较牛、成人及16~20周龄人胎儿中枢神经系统受体活性为高。~(32)P-cDNA探针杂交分析亦显示人胎儿脑组织中含有较高水平的LDL受体mRNA,提示胎儿中枢神经系统正处于生长发育阶段,故对胆固醇需求较高。另外,本文还初步观察到人胎肝及肾上腺皮质LDL受体活性有随胎龄增加而逐渐升高的趋势。

 
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