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p radiolabelled
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     32.
     32。
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     u32.
     u32实现多媒体课件初始化设计的方法。
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     32CasesofTineaManuumTreatedbyQINsExternalTherapyLIUHuaZHANGXue
     秦氏外治法治疗鹅掌风32例
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     32and winapi.
     u32和winapi.
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     DETERMINATION OF ~(32)P IN URINE
     尿中~(32)P的测定
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  p radiolabelled
The RNA, after transfer to Hybond N filters, was probed with 32P-radiolabelled xynB.
      
Intrinsically, 32P-radiolabelled LPS prep arations of the three strains were also prepared in parallel.
      


The ~(15)N,~(32)P radiolabelled study proved that the differences of N,Pelements by millet plant in milking stage were significant among variousvarieties.In late variety“Japan 60-day”and its filial generations theamount of absoring capacity was quite large,but the translocation velo-city towards the ear was slow.The early variety “Xinnong 761”showedlower absorbing capacity,but the translocation velocity towards the earwas fast.And this characteristic can be transmited to the filial genera-tions.The translocation...

The ~(15)N,~(32)P radiolabelled study proved that the differences of N,Pelements by millet plant in milking stage were significant among variousvarieties.In late variety“Japan 60-day”and its filial generations theamount of absoring capacity was quite large,but the translocation velo-city towards the ear was slow.The early variety “Xinnong 761”showedlower absorbing capacity,but the translocation velocity towards the earwas fast.And this characteristic can be transmited to the filial genera-tions.The translocation velocity of tracers ~(15)N,~(32)P towarde the ear hadno correlation with the temperature change,but it had positive correla-tion with the accumulation velocity of dry matter in the ear.Under theconditions of higher contents of effective N,P in the soil the top appli-cation of N,P fertilizers to millet plant before heading didn't promotethe increase of output signally.

用同位素~(15)N、~(32)P 示踪研究证明,谷子灌浆阶段植株对 N、P 元素的吸收量各品种之间有较大的差异,多花晚熟型品种日本60日谷及其杂交后代吸收量较大,但向穗部的运转速度较慢;早熟型新农761谷吸收量虽少,而向穗部运转快,并且这种特性可以遗传给其杂交后代;示踪~(15)N、~(32)P 向穗部的运转速度与当时的气温变化没有相关性,与穗部干物质积累速度呈正相关,在土壤有效N、P 含量较高的条件下,谷子抽穗前追施 N、P 肥料增产作用不大。

Double-stranded replicative form (RF) DMA of mink enteritis virus(MEV) was extracted and purified. A 0.7 kb DNA fragment C obtained by Hind I digestion of MEV RF DNA was cloned into a plasmid vector pBR322. The recombinant pBM was purified from transformed Escherichia coll strain RRI, and fragment C was recovered from pBM. pBM and fragment C were labelled with [α-32P] dATP, and pBM with photobiotin. By dothybridization, 5 members of carnivore parvoviruses and 5 virus strains of non-parvoviridae in infected...

Double-stranded replicative form (RF) DMA of mink enteritis virus(MEV) was extracted and purified. A 0.7 kb DNA fragment C obtained by Hind I digestion of MEV RF DNA was cloned into a plasmid vector pBR322. The recombinant pBM was purified from transformed Escherichia coll strain RRI, and fragment C was recovered from pBM. pBM and fragment C were labelled with [α-32P] dATP, and pBM with photobiotin. By dothybridization, 5 members of carnivore parvoviruses and 5 virus strains of non-parvoviridae in infected cell cultures and 33 fecal samples from healthy or non-infected minks and dogs were examined. Photobiotin-labelled pBM probe and 32P-radiolabelled pBM and fragment C probes had the same specificity. All the carnivore parvovirus infected cell cultures and fecal samples showed positive, and those from healthy minks and dogs were negative. 32P-radio-labelled probes and photobiotin-labelled probe detected 1 pg and 10 pg MEV RF DNA respectively, i.e.100 times and 10 times more sensitive than the standard hemagglutination test.

将提纯的貂肠炎病毒(MEV)中间复制型DNA(RF-DNA),以HinaⅡ酶切,回收0.7kbC片段并克隆至质粒pBR322中,构成重组质粒pBM,经转化大肠杆菌RRⅠ并扩增后,提纯pBM,酶切电泳回收克隆的C片段,以[α-32P]dATP标记C片段和pBM,用光生物素标记pBM,分别制成放射性同位素和光生物素核酸探针。采用打点杂交技术检测了5种肉食兽细小病毒的细胞培养物和非细小病毒科5种病毒的细胞培养物,同时检测了貂和犬的粪便样品33份。结果表明,32P标记的C片段和pBM探针与光生物素际记的pBM探针均具有相同的杂交特异性,与5种肉食兽细小病毒及感染细小病毒的貂、犬粪样呈杂交阳性反应,与其他科病毒及健貂、犬粪样呈阴性。同位素32P和光生物素标记探针分别检出1 pg和10 pg貂肠炎病毒RF-DNA,敏感性比血凝试验分别离100倍和10倍。

To explore the effect of Tiangui Recipe (TGR) on obesity and anovulation in androgen-sterilized rats (ASR) from the gene transcription level. Methods: In situs hybridization and autoradiography were adopted, using α-32 P-radiolabelled leptin receptor (OB-R) and neuropeptide Y (NPY) oligonucleotides probes and integrating with image pattern analysis, to observe the effect of TGR on changes of OB-R, NPY mRNA expression in the hypothalamic arcuate nucleus (ARC). Results: Compared to the normal rats,...

To explore the effect of Tiangui Recipe (TGR) on obesity and anovulation in androgen-sterilized rats (ASR) from the gene transcription level. Methods: In situs hybridization and autoradiography were adopted, using α-32 P-radiolabelled leptin receptor (OB-R) and neuropeptide Y (NPY) oligonucleotides probes and integrating with image pattern analysis, to observe the effect of TGR on changes of OB-R, NPY mRNA expression in the hypothalamic arcuate nucleus (ARC). Results: Compared to the normal rats, the levels of hypothalamic OB-R mRNA expression decreased, NPY mRNA expression elevated significantly in ASR (P < 0.01 ).ASR characterized by obesity and anovulation. There was no significant difference in hypothalamic OB-R, NPY mRNA expressions between normal rats and ASR with TGR administration. Conclusion: The increased hypothalamic NPY and decreased OB-R mRNA expression may be important contributing factors to the development of obesity and anovulation in ASR. TGR may play a role in reducing weight and inducing ovulation by regulating NPY/OB-R mRNA expression.

目的:从基因转录水平探讨中药天癸方对雄激素致不孕大鼠(ASR)肥胖及无排卵的影响。方法:采用α-32P标记瘦素(leptin)受体(OB-R)及神经肽Y(NPY)寡核苷酸探针原位杂交、放射自显影技术结合图像分析,观察中药治疗前后下丘脑弓状核(AR)两种物质mRNA的表达变化。结果:ASR中ARC的NPY基因表达水平明显增加(P<0.01),OB-R基因表达水平明显降低(P<0.01),ASR呈肥胖、无排卵状态。用中药天癸方治疗后,NPY基因表达下降,OB-R基因表达上升至正常大鼠水平。结论:ASR肥胖及无排卵可能与NPYmRNA过度表达、OB-RmRNA数目异常有关,中药可调节NPY和OB-RmRNA表达而发挥减肥及促排卵作用。

 
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