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   anti p 53 的翻译结果: 查询用时:0.164秒
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anti p
相关语句
  抗p53蛋白
     Development of ABC-ELISA method for detecting anti P53 antibodies and its primary clinical application
     检测抗P53蛋白抗体ABC-ELISA法的建立和初步临床应用
短句来源
     Methods we detected P53 proteins and anti P53 antibodies in sera of lung cancer patients and control subjects by western blot technique;
     方法应用Westernblot方法.对28例肺癌患者和18例对照的血清中的P53蛋白和抗P53蛋白抗体进行检测。
短句来源
     Immunohistochemical staining was performed by using LsAB method with anti p53 monoclonal antibody (DAKO DO 7) and prolife rating cell nuclear antigen (PCNA, DAKO clone PC10) antibody as primary antibodies.
     用抗p53蛋白(DAKODO7)和抗增殖细胞核抗原(PCNA,DAKOclonePC10)的单抗,采用LsAB免疫组化法染色。
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  “anti p 53”译为未确定词的双语例句
     The 26 kD band was detected by anti p53 antibodies.
     26kD蛋白可被抗p53抗体识别;
短句来源
     Conclusions The first domestic product of anti p53 monoclonal antibody,M126,is very useful in investigating the overexpressiono f p53 in bladder cancer as well as in other malignant neoplasms.
     结论作为第一株国产抗p53单抗,M126将在肿瘤p53表达和突变的研究中发挥重要的作用
短句来源
     The present study also compared the immunoreactivity of M126 with other anti p53 monoclonal antibodies such as PAb 1801,and demonstrated that M126 not only recognized transitional cell carcinoma cells with high specificity and reactivity,but also stained p53 strongly with or without microwave oven treating.
     与进口单抗PAb1801相比较,M126识别不同的抗原表位,染色强度和阳性细胞指数均明显增高,而且对不经微波修复的抗原也有很好的反应性。
短句来源
     Methods:Using immunostaining with anti P16 poly clonal antibody and anti P53 monoclonal antibody,we detected the expression of P16 and P53 in 28 cases of pancreatic adenocarcinoma and 6 cases of normal pancreatic tissue. Proliferating cell nuclear antigen (PCNA) was also studied.
     方法:应用免疫组化 L S A B法研究28例胰腺癌组织中 P16蛋白和 P53蛋白的表达情况,并进行增殖细胞核抗原( P C N A)检测,计算细胞增殖指数( Proliferation index, P I)。
短句来源
     Methods Expression of CD44 and p53 genes was immunohistochemically detected in 78 gastric carcinomas and 46 benign gastric mucosa lesions adjacent to carcinoma by monoclonal antibodies of 2F10 (anti CD44V6) and DO 1 (anti p53).
     方法 运用免疫组化法 ,检测 78例原发胃癌及癌旁良性病变的CD4 4V6及p53基因蛋白的表达。
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  相似匹配句对
     Anti-H.
     结果表明,抗H.
短句来源
     Anti-H.
     治疗结果显示H.
短句来源
     p53 is an important anti-oncogen.
     p53是一种重要的抑癌基因。
短句来源
     p53-selective anti-tumor adenovirus
     p53选择性抗瘤腺病毒
短句来源
     The alterations of p53 gene in cells induced by anti-BPDE
     反式二氢二醇环氧苯并芘诱发细胞p53基因的变化
短句来源
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  anti p
We also carried out an immunohistochemical study with anti p53 antibody on the goblet cell carcinoid tumor of the appendix.
      
We examined the endogenous p53 protein status in CH31 cells by performing immunoblot analysis using the anti-p53 mAb.
      
We showed a trend towards a poorer prognosis of anti-p53 antibody positive patients with breast cancer or hepatocellular carcinoma.
      
We investigated circulating anti-p53 antibodies in the plasma of Brazilian brain tumor patients.
      
We then tested whether these anti-DNA antibodies, like the p53 carboxy terminus itself, might bind the anti-p53 PAb-421 antibody.
      
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The schizonts ( merozoites ) of plasmodium falciparum were iodinated by 125I-lactoperoxidase method and the labelled parasites extracted in Triton X-100. The extract was then either precipitated by mouse anti-P falciparum monoclonal antibody (McAb)94D1 or purified through an immunoabsorbent column prepared with the same monoclonal antibody.The results showed that this monoclonal antibody precipitates three polypeptides of 55kd,48 kd and 25 kd in molecular weight, which are identical to those purified...

The schizonts ( merozoites ) of plasmodium falciparum were iodinated by 125I-lactoperoxidase method and the labelled parasites extracted in Triton X-100. The extract was then either precipitated by mouse anti-P falciparum monoclonal antibody (McAb)94D1 or purified through an immunoabsorbent column prepared with the same monoclonal antibody.The results showed that this monoclonal antibody precipitates three polypeptides of 55kd,48 kd and 25 kd in molecular weight, which are identical to those purified by using immunoabsorbent technique.

用~(125)Ⅰ—乳过氧化物酶法标记恶件疟原虫裂殖体(子),TritonX—100提取疟原虫表面标记抗原。抗恶性疟单克隆抗体9_4D_1与上述恶性症原虫膜抗原提取物作免疫沉淀分析,并用单克隆抗体9_4D_1制备免疫吸附剂,通过亲和层析自恶性疟原虫提取物中分离纯化相应的靶抗原。结果表明,免疫沉淀的靶抗原分子量为55kd,48kd和25kd的多肽,与亲和层析纯化出的靶抗原基本一致.

Hybridoma cell lines secreting monoclonal antibody ( MCA ) to avian leukosis virus ( ALV ) structural proteins p27 and p19 have been established. MCA 6AL20 ( IgG1 isotype ) reacted with RPL-40, AMV, RAV-2 and Carr-Zilber RSV ( CZ-RSV ) .representing exogenous ALV subgroups A, B and D, respectively, but not the endogenous virus RAV-0 ( subgroup E ) in an indirect enzyme-linked immunosorbent assay ( ELISA ) . MCA 6AL22 reacted as above and, in addition, reacted with the Prague strain of Rous sarcoma...

Hybridoma cell lines secreting monoclonal antibody ( MCA ) to avian leukosis virus ( ALV ) structural proteins p27 and p19 have been established. MCA 6AL20 ( IgG1 isotype ) reacted with RPL-40, AMV, RAV-2 and Carr-Zilber RSV ( CZ-RSV ) .representing exogenous ALV subgroups A, B and D, respectively, but not the endogenous virus RAV-0 ( subgroup E ) in an indirect enzyme-linked immunosorbent assay ( ELISA ) . MCA 6AL22 reacted as above and, in addition, reacted with the Prague strain of Rous sarcoma virus ( PrC-RSV ) , subgroup C. Both MCAs im-munoprecipitated p19 from 35S-methionine-labeled RPL-40 or RAV-1,but not RAV-O infected chi-cken embryo fibroblasts ( CEF ) . They can be used to differentiate exogenous from endogenous ( RAV-O ) infection either in an indirect antibody ELISA or by immunoprecipitation. A third MCA, 6AL42 ( IgG2a isotype), reacted with exogenous viruses at an antibody titer up to 1,000-fold higher than with endogenous subgroup E, RAV-O virus in indirect ELISAs. MCA 6AL42 immunoprecipitated p27 and the group-specific antigen precursor protein, pr76, from cells infected with RPL-40, RAV-1 or RAV-O. A double antibody sandwich ELISA, using 6AL42 as the primary binding antibody and conjugated rabbit anti-p27 as the reporter antibody, differentiated between endogenous RAV-O and exogenous p27 antigen in undiluted albumen samples.

建立了分泌抗禽白血病毒(ALV)结构蛋白P~(27)和P~(19)的单克隆抗体(McAb)杂交瘤细胞。酶联免疫吸附试验(ELISA),McAb-6AL20(属于IgG_1)分别与外源性ALV:A.B.D亚群的RPL-40、AMV、RAV-2和Carr-Zilber RSV(CZ-RSV)发生特异性反应,而不与内源性ALV:RAV-O(E亚群)发生反应。McAb-6AL_(22)(属于IgG_1)除了上述特性外,还能与劳斯氏肉瘤病毒Prague株(Prc-RSV、属于C亚群)发生反应。这二个McAb均能与〔~(35)S〕甲硫氨酸标记的RPL-40和RAV-1病毒的P~(19)蛋白出现免疫沉淀。但是,它们不与RAV-O病毒的P~(19)蛋白发生免疫沉淀。所以,McAb可用于区别ALV结构蛋白P~(19)(亚群特异性位点的多肽)。应用这二个McAb进行ELISA和免疫沉淀与聚丙烯酰胺凝胶电泳试验可区分RPL-40和RAV-O感染。McAb-6AL42(属于IgG2a)可与以上几个亚群(A.B.C和D)的毒株发生反应,其ELISA的抗体滴度比与RAV-O(E亚群)毒株高1000倍以上。McAb-6AL42可与P~(2...

建立了分泌抗禽白血病毒(ALV)结构蛋白P~(27)和P~(19)的单克隆抗体(McAb)杂交瘤细胞。酶联免疫吸附试验(ELISA),McAb-6AL20(属于IgG_1)分别与外源性ALV:A.B.D亚群的RPL-40、AMV、RAV-2和Carr-Zilber RSV(CZ-RSV)发生特异性反应,而不与内源性ALV:RAV-O(E亚群)发生反应。McAb-6AL_(22)(属于IgG_1)除了上述特性外,还能与劳斯氏肉瘤病毒Prague株(Prc-RSV、属于C亚群)发生反应。这二个McAb均能与〔~(35)S〕甲硫氨酸标记的RPL-40和RAV-1病毒的P~(19)蛋白出现免疫沉淀。但是,它们不与RAV-O病毒的P~(19)蛋白发生免疫沉淀。所以,McAb可用于区别ALV结构蛋白P~(19)(亚群特异性位点的多肽)。应用这二个McAb进行ELISA和免疫沉淀与聚丙烯酰胺凝胶电泳试验可区分RPL-40和RAV-O感染。McAb-6AL42(属于IgG2a)可与以上几个亚群(A.B.C和D)的毒株发生反应,其ELISA的抗体滴度比与RAV-O(E亚群)毒株高1000倍以上。McAb-6AL42可与P~(27)和pr~(76)(群特异性抗原的前体蛋白)发生免疫沉淀。试验用McAb-6AL42作为第一抗体和兔抗P~(27)血清的过氧化物酶标记物作为第二抗体(指示抗体)建立了双抗体夹心ELISA试验。用这种ELISA试验可区分未经稀释的卵蛋白中RAV-O和RPL-40群特异性抗原。

The free or carrier-linked immunogens were prepared from three synthetic peptides correspondeing to the sequence of hypatitis B surface antigen.These immunogens were injected into rabbits,and most them elicited an antipeptide response.Antisera against P122-148(ayw)and P122-148(adw)were rective to native HBsAg as shown by AUSAB test,in which the anti-P122-148(adw) sera showed higher reactivity than other antipeptide antibodies ever reported.Structural analysis indicated that immunogens...

The free or carrier-linked immunogens were prepared from three synthetic peptides correspondeing to the sequence of hypatitis B surface antigen.These immunogens were injected into rabbits,and most them elicited an antipeptide response.Antisera against P122-148(ayw)and P122-148(adw)were rective to native HBsAg as shown by AUSAB test,in which the anti-P122-148(adw) sera showed higher reactivity than other antipeptide antibodies ever reported.Structural analysis indicated that immunogens containing the immunodominant regions of native proteins would be ideal candidates for preparation of efficient vaccines by synthetic methods.

我们将三种乙型肝炎表面抗原的合成肽段在联结载体或不接载体的情况下,分别制备成4种免疫原对兔子进行了免疫。大部分兔子都产生了抗肽抗体。其中抗P_(122-48)(auw)与抗P_(122-148)(adw)都能与天然HBsAg反应,而抗P_(122-148)(adw)的抗HBs活力比文献报道的都高,通过结构分析表明:含有天然蛋白上免疫显性区域的免疫原是合成疫苗的理想选者。

 
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