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et recombination
相关语句
  et重组
     Red/ET Recombination and its Biomedical Applications
     Red/ET重组及其在生物医学中的应用
短句来源
     ET Recombination: new options for cloning DNA in Escherichia coli
     ET重组:Escherichia coli中最新分子克隆方法
短句来源
     The Application of Red/ET Recombination to High Efficient Gene-targeting Vector Construction
     Red/ET重组在基因打靶载体快速构建中的应用
短句来源
     Red/ET recombination, a powerful homologous recombination system based on the Red operon of λ phage or RecE/RecT from Rac phage, provides an innovative approach for DNA engineering.
     通过应用Rac噬菌体的RecE RecT和λ噬菌体的RedαRedβ系统而建立的DNA工程平台———Red ET重组,是一种不依赖于限制性内切酶的分子克隆新技术。
短句来源
     Red/ET recombination has the advantage of getting longer homology regions without mutation,which makes it a new and reliable alternative to the construction of a targeting vector today.
     因此Red/ET重组为构建打靶载体提供了一种新的可靠的方法。
短句来源
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  “et recombination”译为未确定词的双语例句
     Rapid Modification of BACs With The Optimized Red/ET Recombination System
     Red/ET同源重组介导细菌人工染色体的快速修饰
短句来源
     New Option for Gene-targeting Vector Construction——Red/ET Recombination
     用Red/ET重组酶构建基因打靶载体
短句来源
     Furthermore, ET recombination can be used for direct subcloning and cloning of DNA from complex mixtures, including bacterial artificial chromosomes and genomic DNA preparations.
     能够直接进行亚克隆并且还可以从细菌人工染色体和基因组DNA片段中克隆所需DNA。
短句来源
     With the completion of genome-sequencing projects, intentional modification of definite bacterial artificial chromosomes (BACs), which carry entire components of most eukaryotic genes, is becoming more important for the subsequent functional genomics studies. The newly optimized Red/ET recombination system was applied to the BAC modification.
     随着基因组测序工程的实施与完成,如何对包含完整基因信息的特定细菌人工染色体(BAC)进行有目的修饰,已成为功能基因组学研究的一个重要环节.
短句来源
     Mediated by the plasmid, pSC101-BAD-gbaA, and assisted by the counter-selectable/selectable system conferred by rpsL-Neo, two BACs were successfully modified. One step selectable BAC modification such as simple deletion or insertion was achieved in one week. For insertion or fusion of unselectable gene such as Cre, EFGP and LacZ as well as point mutation into targeted BACs, two rounds of Red/ET recombination was proceeded and two weeks was needed.
     应用新近优化的Red/ET同源重组技术对目标BAC进行修饰,以pSC101-BAD-gbaA为依托质粒,采用rpsL鄄neo为正/反向筛选系统,可以快速、高效地对BAC进行剪切、插入、替换等操作,其中能够进行抗性筛选的一步BAC修饰只需一周时间,以插入非抗性标记基因Cre为代表的两步BAC修饰在两周内即可完成.
短句来源
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  相似匹配句对
     et Flah.
     Bornet et Flahault ],S. bohnei Schmidle,S.
短句来源
     Property Recombination
     物业管理企业资产重组浅析
短句来源
     Go. M et.
     GO.
短句来源
     recombination of signals.
     信号重组。
短句来源
     The characteristic and development of Red/ET recombination and its biomedical applications were described in this review.
     结合自身的一些研究结果,对Red ET重组的技术特点、发展现状和在生物医学中的应用进行了详细阐述。
短句来源
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  et recombination
The resulting strain was used as the host for Red/ET recombination.
      
To accomplish this goal, an approach using Red/ET recombination with gene complementation was developed.
      
By using Red/ET recombination technology, we were able to circumvent many of the present limitations in the engineering of polyketide systems.
      
Because the BAC that we used contained several genes, we trimmed it by ET recombination12 to carry Hc only.
      


Driven by the needs of functional genomics, DNA engineering by homologous recombination in Escherichia coli has emerged as a major addition to existing technologies. ET Recombination allows a wide variety of DNA cloning and provides a convenient and flexible method, which cannot be accomplished by conventional method. ET Recombination does not rely on the presence of suitable restriction sites and can be used to insert, delete, or substitute for DNA sequence at any desired position on a...

Driven by the needs of functional genomics, DNA engineering by homologous recombination in Escherichia coli has emerged as a major addition to existing technologies. ET Recombination allows a wide variety of DNA cloning and provides a convenient and flexible method, which cannot be accomplished by conventional method. ET Recombination does not rely on the presence of suitable restriction sites and can be used to insert, delete, or substitute for DNA sequence at any desired position on a target molecule. Furthermore, ET recombination can be used for direct subcloning and cloning of DNA from complex mixtures, including bacterial artificial chromosomes and genomic DNA preparations. The strategies reviewed in this article are applicable to modification of DNA molecules of any size, including very large ones, and present powerful new avenues for DNA manipulation in general.

功能基因组研究的飞速发展推动了在E .coli中运用体内同源重组的方法进行DNA操作的技术革新步伐 ,ET体内重组方法具有应用范围广、操作灵活简便、重组效率高等优点 ,它可进行常规分子生物学无法实现的操作。这种方法不依赖于合适的限制酶切位点 ,能够在靶分子的任意位置上进行基因的插入、缺失和替换 ;能够直接进行亚克隆并且还可以从细菌人工染色体和基因组DNA片段中克隆所需DNA。本文所阐述的方法可应用于包括长DNA在内的任意大小DNA的修饰 ,并概括描述了基因调控研究的最新策略

With the completion of genome-sequencing projects, intentional modification of definite bacterial artificial chromosomes (BACs), which carry entire components of most eukaryotic genes, is becoming more important for the subsequent functional genomics studies. The newly optimized Red/ET recombination system was applied to the BAC modification. Mediated by the plasmid, pSC101-BAD-gbaA, and assisted by the counter-selectable/selectable system conferred by rpsL-Neo, two BACs were successfully modified. One...

With the completion of genome-sequencing projects, intentional modification of definite bacterial artificial chromosomes (BACs), which carry entire components of most eukaryotic genes, is becoming more important for the subsequent functional genomics studies. The newly optimized Red/ET recombination system was applied to the BAC modification. Mediated by the plasmid, pSC101-BAD-gbaA, and assisted by the counter-selectable/selectable system conferred by rpsL-Neo, two BACs were successfully modified. One step selectable BAC modification such as simple deletion or insertion was achieved in one week. For insertion or fusion of unselectable gene such as Cre, EFGP and LacZ as well as point mutation into targeted BACs, two rounds of Red/ET recombination was proceeded and two weeks was needed. After L-arabinose induction for transient expression of red/red?茁/red?琢/recA, the pSC101-BAD-gbaA plasmid died out spontaneously from the BAC host bacteria by shifting the temperature from 30℃ to 37℃. Thus, there was no DNA contamination in the modified BACs being used for subsequent transgenesis research. The high efficient BAC modification mediated by optimized Red/ET recombination offers a significant facility to the functional genomics investigations.

随着基因组测序工程的实施与完成,如何对包含完整基因信息的特定细菌人工染色体(BAC)进行有目的修饰,已成为功能基因组学研究的一个重要环节.应用新近优化的Red/ET同源重组技术对目标BAC进行修饰,以pSC101-BAD-gbaA为依托质粒,采用rpsL鄄neo为正/反向筛选系统,可以快速、高效地对BAC进行剪切、插入、替换等操作,其中能够进行抗性筛选的一步BAC修饰只需一周时间,以插入非抗性标记基因Cre为代表的两步BAC修饰在两周内即可完成.通过阿拉伯多糖诱导调控和简单地变化培养温度,能使pSC101-BAD-gbaA依托质粒在发挥完Red/ET同源重组作用后自然消失,最终获得完整而纯净的修饰后BAC,为加快功能基因组学研究提供了一个可靠的实验平台.

Red/ET recombination, a powerful homologous recombination system based on the Red operon of λ phage or RecE/RecT from Rac phage, provides an innovative approach for DNA engineering. Deletion, insertion and mutation can be quickly and precisely performed on the target gene mediated by Red/ET recombination with PCR derived DNA fragments or oligonucleotides. This technical platform has extensive applications in biomedical field including bacterial artificial chromosome modification, gene knock-out...

Red/ET recombination, a powerful homologous recombination system based on the Red operon of λ phage or RecE/RecT from Rac phage, provides an innovative approach for DNA engineering. Deletion, insertion and mutation can be quickly and precisely performed on the target gene mediated by Red/ET recombination with PCR derived DNA fragments or oligonucleotides. This technical platform has extensive applications in biomedical field including bacterial artificial chromosome modification, gene knock-out construction and genetic modification of E.coli strains as well as some other kinds of microorganisms. Recently, Red/ET recombination was improved in several aspects so that it becomes more powerful and maneuverable. The characteristic and development of Red/ET recombination and its biomedical applications were described in this review.

通过应用Rac噬菌体的RecE RecT和λ噬菌体的RedαRedβ系统而建立的DNA工程平台———Red ET重组,是一种不依赖于限制性内切酶的分子克隆新技术。运用该技术能够介导PCR产物或寡核苷酸对目标基因进行剪切、插入、融合及突变等多种操作,在生物医学领域里具有广阔的应用前景,尤其在基因组功能研究中对BACs、PACs和细菌染色体的打靶修饰以及基因敲除动物DNA靶分子的快速构建等方面最有效。随着Red ET重组的推广与应用,该技术本身也在不断被改进,在工作效率得到显著提高的同时,其操作也变得更加简单、省时、省力。结合自身的一些研究结果,对Red ET重组的技术特点、发展现状和在生物医学中的应用进行了详细阐述。

 
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