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lectin-binding
相关语句
  凝集素结合
     Lectin-binding Characteristics in the Endometrium of Sheep
     绵羊子宫内膜的凝集素结合特性
短句来源
     Lectin-binding characteristics of related high-and-low metastatic mouse hepatocarcinoma cell lines
     高和低转移能力的小鼠肝癌细胞凝集素结合的组化观察
短句来源
     Lectin-binding Characteristics of Glycoproteins of Related High-and Low-Metastatic Murine HCa Cell Lines
     高及低转移潜能小鼠肝癌细胞株糖蛋白凝集素结合特征的研究
短句来源
  “lectin-binding”译为未确定词的双语例句
     The results showed that CMC posseed lectin-binding sites for ConA,WGA, PCA, PSA, BSL.
     结果显示:ConA、WGA、RCA、PSA、BSL对该组织有反应。
短句来源
     Study on lectin-binding expression and lymph node metastasis of rectal carcinoma in the young
     青年人直肠癌淋巴结转移与凝集素受体表达的研究
短句来源
     For better understanding of the significance of β-gal glycoconjugates distribution during gametogenesis, the distribution of Ricinus communis agglutinin Ⅰ (RCA-Ⅰ) receptors during gametogenesis in Atractomorpha sinensis Bolivar was detected by using lectin-binding cytochemistry method.
     利用凝集素细胞化学方法对短额负蝗Atractomorpha sinensis Bolivar配子发生过程中蓖麻凝集素(Ricinus communis agglutininⅠ,RCA-Ⅰ)受体分布进行了定位研究,旨在认识昆虫配子发生过程中β-半乳糖复合物的分布规律及意义。
短句来源
     Abstract The cartilage of mandibular condyle (CMC) of 43 human fetuses, ranging from 13 to 38 weeks, was studied with lectin-binding histochemical technics (9 biotin-labelled lectins).
     作者应用凝集素亲和组化法(九种凝集素)对43例13周至38周的髁状突软骨(CMC)进行研究,目的是观察胎儿CMC各部位的特异性糖基及其随胎龄增长的变化。
短句来源
     To study glycoconjugates distributions in developing retina of human, developing retinas of human were studied with lectin-binding histochemistry usded biotin-couple WGA、 ConA、 PNA.
     选择3种生物素标记的凝集素:麦胚凝集素(WGA)、伴刀豆凝集素(ConA)、花生凝集素(PNA),采用ABC组织化学法对正常人发育视网膜中糖结合物的分布进行研究。
短句来源
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  相似匹配句对
     Lectin Research in China
     中国的来克丁研究
短句来源
     Studies on Lectin of Pseudomonas Aeruginose
     绿脓杆菌凝集素(Lectin)的研究:Ⅰ凝集素的产生与对上皮细胞的粘附和蛋白酶分泌的关系
短句来源
     Mammalian C-Type Lectin Superfamily.
     哺乳类C型凝集素超级家族
短句来源
     Cloning and Expression of Lectin of Human P-selectin
     人P选择素lectin基因片段的克隆及表达
短句来源
     Lectin-binding Characteristics in the Endometrium of Sheep
     绵羊子宫内膜的凝集素结合特性
短句来源
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  lectin-binding
The mobility of only the lectin-binding disaccharide is reduced affording a separation in the first-dimension.
      
Using ferritin-labelledRicinus communis agglutinin to detect lectin-binding sites of the pancreatic B-cell surface, we show that limited regions of the plasma membrane are deprived of lectin-binding sites over marginated secretory granules.
      
A survey of the binding sites of twelve lectins to the surface of juvenile and adult females, and adult males demonstrated localized lectin-binding at sites considered important in mating behavior of each sex/age class.
      
Lectin-binding patterns on the body surface of females have indicated that surface-bound glycoproteins may be important signals in mate choice.
      
In this report, we show that New Zealand black (NZB) mice express a variant CD23 allele, with mutations in both the C-lectin-binding domain and stalk region, which fails to bind IgE at high affinity and has reduced expression on the cell surface.
      
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Receptors of wheat germ agglu-tinin (WGA) and soybean agglutinin (SBA) are present on the denuded living egg surface of Rana amurensis.Before cleavage these receptors dis-tribute evenly all over the animal pole (Plate I.Fig.1).At the onset of cleavage,numerous bright fluores-cent granules which correspond to the protrusions in the scanning ele-ctron microscope (10),are seen on the cleavage stripe and many bright ra-diated rays which is identified as folds,are visible on the side of the cleavage stripe (Plate...

Receptors of wheat germ agglu-tinin (WGA) and soybean agglutinin (SBA) are present on the denuded living egg surface of Rana amurensis.Before cleavage these receptors dis-tribute evenly all over the animal pole (Plate I.Fig.1).At the onset of cleavage,numerous bright fluores-cent granules which correspond to the protrusions in the scanning ele-ctron microscope (10),are seen on the cleavage stripe and many bright ra-diated rays which is identified as folds,are visible on the side of the cleavage stripe (Plate I.Fig.2).Soon after,the new membrane hav-ing much less FITC-lectins binding ability,appears and divides the clea-vage stripe into 3 bright lines.The middle one is the primitive contrac-tile ring.While the cleavage goes on,the new membrane increases gra-dually (Plate I.Fig.3).However,when the denuded eggs are immersed in high concentration of WGA (500γ/ml) before cleavage,the new mem-brane appears only for a short time,then vanishes,leaving a black streak (Plate I.Fig.6 b,d).These results are in line with those found in newt's eggs (5).In high concentration of SBA (100 γ/ml) the new membrane exposed continuously but in a much smaller schedule.The contractile ring initially working normally,fails to work,and the new membrane bulges out (Plate II.Fig.7 & 8).Finally the new membrane disappears as those in WGA (Plate.I.Fig.6 a & c are untreated control). In a few FITG-SBA stained eggs,a large dark halo,its center often coincides with the center of the furrow,appears quickly (Plate II.Fig.12),and persists for several minutes to more than one hour,so that it may overlap with the halos seen on the second cleavage stripes.The darker overlapping area gradually decreases and disappear eventually while the ??halos on the second cleavage furrows move away.Sometimes a bright halo may be seen on the egg surface.As the halo contracts,it becomes brighter and brighter.Finally many radiated stripes and a nipple-like structure are found in the center of the halo (Plate II.Fig.10-11).A few receptors,however,remains or lags behind dur-ing the contraction of the bright halo.This indicates that the receptor can become mobile or increase its moving rate under certain condition.The increasing of fluorescence,however,is not always resulting from the mo-vement of the receptor but from the movement of cell surface.By push-ing the evenly distributed fluorescent egg surface,many brighter lines appear at the folded places.This is explain-ed in figure 1.Increasing in fluores-cent intensity due to the fold of egg surface is also present naturely suchas the bright radiated rays and gra-nules at the cleavage stripe. Fig.1.Digrammatical representative of the dif-ferent intensity of fluorescence on the plain and fold region."A" Plain region."B" Fold or protuberant region."." FITC-lectin binding site."I" Transverse section,binding sites inequal distance."II" Top view.Binding sites becoming dense or fluorescence becoming brighter in B region.Indicating the increase of the intensing of fluo-rescence resulting from the move-ment of the binding sites itself.

林蛙卵表面有WGA和SBA受体,胞质分裂前受体分布均匀,开始分裂时预定分裂沟处出现明亮的光带,此时或稍后,在FITC-SBA染的卵中,少数细胞表面会出现一个以分裂沟中心为圆心的暗晕。暗晕的直径刚出现时就有近半个卵球那么大,以后并不明显增大,暗晕的出现和消失相当快,存在的时间长短不一(有的长达1—2小时),第二次分裂沟上也会出现暗晕,当第二次分裂沟的一端向外延伸时,整个暗晕也随之外移。在某种情形下,细胞膜上的受体可移向某一中心,出现亮晕。高浓度的WGA和SBA能使分裂沟中的新膜很快消失,分裂沟成为线状痕迹。高浓度的SBA还能使收缩环逐渐失去作用,新膜随之突出。

The influence of some saccharides, glycosides, glycoproteins and lectins on the binding of ~(125)I-labelled Trichosanthes lectin with acidtreated Sepharose 6B or human placental cell membrane were compared. At low concentrations, among saccharides so far tested, lactose was the strongest inhibitor; melibiose and raffinoae were as strong as the monosaccharide galactose; cellobiose, maltose and sucrose had no effect. Three glycoproteins have been tested, their inhibitory capacities in decreasing order were: porcine...

The influence of some saccharides, glycosides, glycoproteins and lectins on the binding of ~(125)I-labelled Trichosanthes lectin with acidtreated Sepharose 6B or human placental cell membrane were compared. At low concentrations, among saccharides so far tested, lactose was the strongest inhibitor; melibiose and raffinoae were as strong as the monosaccharide galactose; cellobiose, maltose and sucrose had no effect. Three glycoproteins have been tested, their inhibitory capacities in decreasing order were: porcine thyroglobulin, human serum transferrin and hen ovalbumin. Unlabelled Trichosanthes leotin and ricin I, another galactose-binding leotin, competed with labelled Trichosanthes lectin; however Con A and Pinellia lectin which bound to glucose/mannose and mannan, respectively, did not. It seems reasonable to conclude that Trichosanthes lectin bound mainly to galactose, and its binding site could fit galactose and another sugar residue, but such a disaccharide had to possess a specific conformation, such as that of lactose.

本文报道一些糖类、糖苷、糖蛋白和几种外源凝集素对标记天花粉凝集素和酸处理交联琼脂糖或胎盘细胞膜结合的影响。在低浓度时,所用的糖类中,乳糖是最强的抑制剂,蜜二糖和棉籽糖的抑制能力和乳糖相仿,而纤维二糖、蔗糖、麦芽糖,则无明显影响。三个所用的糖蛋白,它们的抑制活性以下列顺序递减:猪甲状腺球蛋白,人血清转铁蛋白,鸡卵白蛋白。未标记的天花粉凝集素和蓖麻凝集素,两者都专一地和半乳糖结合,它们都能竞争标记天花粉凝集素,而伴刀豆球蛋白A和半夏凝集素则不能竞争。由此,我们推测天花粉凝集素主要是和半乳糖结合,但与乳糖的结合能力最强,故推测其结合部位能容纳半乳糖和另一个单糖。

Squash (Cucurbita moschata Duch.) stigma was of the dry-type. When it wastreated with detergent, proteinase and lectin, pollen could not germinate, or pollentube growth was arrested.The binding of Con A-FITC to the stigma surface indicatedthe presence of lectin-binding sites which midght play an important role in the pollen-pistil interactions. The squash stigma segments stimulated pollen germination invitro, which suggested the presence of certain stimulants in squash stigma tissue. When squash stigma...

Squash (Cucurbita moschata Duch.) stigma was of the dry-type. When it wastreated with detergent, proteinase and lectin, pollen could not germinate, or pollentube growth was arrested.The binding of Con A-FITC to the stigma surface indicatedthe presence of lectin-binding sites which midght play an important role in the pollen-pistil interactions. The squash stigma segments stimulated pollen germination invitro, which suggested the presence of certain stimulants in squash stigma tissue. When squash stigma was pollinated with pollen from plants of different speciesother than squash, the pollen (tube) growth was arrested at different sites. Thehemagglutination activity was detected in the mature pistil, while no activity wasfound in the immature one. The extract of pistil inhibited the germination of tobac-co (Nicotiana tabacum) pollen. The inhibition could be relieved by complementarysugars oflectin. It was suggested that pistil lectin might be involved in the inhibitionof tobacco pollen tube by squash pistil. To investigate the action of lectin, exogenous lectin was applied. It was foundthat Con A inhibited the germination of squash pollen and the inhibitory effect wasremoved by α-methyl-D-mannoside. When pollen was labeled with Con A-FITC,the label was bound to the pollen tube. Con A-FITC was also bound to the isolatedpollen wall fragment, but no fluorescence was observed after it was treated withalkali. It was concluded that lectin might inhibit pollen germination by bindingto the glycoprotein or polysaccharide components of the pollen wall.

南瓜柱头表面经去垢剂、蛋白酶及Con A处理后花粉不能萌发或花粉管生长受阻,Con A能专一地与柱头表面结合。柱头块加入培养液可促进花粉萌发。不同的远缘花粉授粉后在雌蕊不同部位受阻。在成熟南瓜雌蕊提取液中检测到血凝活性,凝集素可能参与雌蕊对远缘花粉的抑制。

 
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