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    HBsAg was positive;
    HBsAg阳性;
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    HbsAg, HbsAb;
    HbsAg、HbsAb;
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    HbsAg, anti-HBc;
    HbsAg,抗 Hbc;
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    In 325 cases in the HBsAg-positive
    对照组325例中阳性57例,感染率为17.54%。
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A method for the localization of HBsAg in liver tissue by means of horseradishperoxidase-labelled antibody(indirect method)under light microscopy is described.It is highly specific and reproducible.

本文报告在光学显微镜下采用辣根过氧化物酶标记抗体(间接法)对肝组织内乙型肝炎表面抗原(HBsAg)的一种定位方法。此法专一性强,重复性好。

A method for the localization of HBsAg in hepatocytes by means of horseradishperoxidase-labelled antibody(indirect method)at electron microscopic level isdescribed.Some problems involved are discussed.The ultrathin sections for electron microscopic examination may be preparedeither after re-embedding of a thick section used in light microscopic examination,in which HBsAg was localized immunocytochemically with horseradish peroxidase-labelled antibody,or by staining immunocytochemically with horseradish...

A method for the localization of HBsAg in hepatocytes by means of horseradishperoxidase-labelled antibody(indirect method)at electron microscopic level isdescribed.Some problems involved are discussed.The ultrathin sections for electron microscopic examination may be preparedeither after re-embedding of a thick section used in light microscopic examination,in which HBsAg was localized immunocytochemically with horseradish peroxidase-labelled antibody,or by staining immunocytochemically with horseradish peroxidase-labelled antibody directly on ultrathin section.The distribution and form of HBsAg in the HBsAg pesitive hepatocyte has beenexamined preliminarily,using horseradish peroxidase-labelled antibody under electronmicroscopy.Under electron microscopic examination there are some deposits of HBsAg withenzyme-labelled antibody,dispersed in the cytoplasma or the expanded cisternae ofthe endoplasmic reticulum of some hepatocytes which were verified light microscopicallyto be HBsAg negative.Electron microscopic examination gives more significantinformation.

本文介绍辣根过氧化物酶标记抗体对肝细胞内乙型肝炎表面抗原(HBsAg)的免疫电镜定位方法。电镜样品制备,可采用酶标免疫显色组织切片的再包埋、或直接利用超薄切片进行酶标免疫显色定位。对实验中应注意的一些问题进行了讨论。在电子显微镜下初步观察了HBsAg 阳性肝细胞中HBsAg 的分布和形态,并发现在光学显微镜下,辣根过氧化物酶标免疫定位HBsAg 完全为阴性部位的肝细胞中,有的细胞胞浆内或扩大的内质网池内,尚可见到少量散在的HBsAg 与酶标抗体的沉积物,提示电镜观察有更好的灵敏性。

The cellular immunity of different types of hepatitis cases was studied by an improved leucocyte migration inhibition test. Using phytohaemagglutinin (PHA) as mitogen stimulator, 53.4% of 133 chronic persistent hepatitis cases. 72.2% of 18 chronic active hepatitis cases, 50% of 12 acute hepatitis cases and all of the 27 healthy controls showed inhibition of leucocyte migration. Using the crude hepatitis B surface antigen (HBsAg) as antigen. 77.2% of 79 hepatitis cases showed inhibition. When liver specific...

The cellular immunity of different types of hepatitis cases was studied by an improved leucocyte migration inhibition test. Using phytohaemagglutinin (PHA) as mitogen stimulator, 53.4% of 133 chronic persistent hepatitis cases. 72.2% of 18 chronic active hepatitis cases, 50% of 12 acute hepatitis cases and all of the 27 healthy controls showed inhibition of leucocyte migration. Using the crude hepatitis B surface antigen (HBsAg) as antigen. 77.2% of 79 hepatitis cases showed inhibition. When liver specific lipoprotein(LPI)was used as antigen. 18.1% of the133 chronic persistent hepatitis cases, 44.4% of 18 chronic active hepatitis cases, 25.0% of 12 active hepatitis cases and 1 in 32 healthy controls showed abnormal responses, in cellular immunity.

应用改进的白细胞移动抑制试验测试不同类型肝炎患者的细胞免疫状态。以植物血凝素(PHA)为刺激因子,133名迁肝患者中有53.4%,18例慢活肝患者中有72.2%,12例急性肝炎患者中有50%呈现白细胞移动抑制,而27名健康人均出现抑制现象。以粗制乙型肝炎表面抗原(HBsAg)为抗原,在79例肝炎患者中,有77.2%出现异常反应,而在25名健康人中只有4%为阳性。以肝细胞膜脂蛋白(LPI)为抗原,133例迁肝中有18.1%,18例慢活肝中有44.4%,12例急性肝炎中有25.0%呈异常反应,而在32名健康人中只有3.1%异常。

 
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