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horseradish peroxidase conjugate
相关语句
  辣根过氧化物酶结合物
     A specific sensitive and rapid ELISA (double antibody sandwich technique) with anti-human IgG and corresponding horseradish peroxidase conjugate was described for the quantitative determination of trace amounts of IgG.
     本文报道了一种特异、敏感,快速的酶联免疫吸附试验(ELISA)双抗体夹心法和抗人IgG及相应辣根过氧化物酶结合物用于测定微量IgG。
短句来源
  “horseradish peroxidase conjugate”译为未确定词的双语例句
     Motoneurons of Musculus tibialis anterior (TaMn) in 99 Wistar rats of age from postnatal day 1 (P1) to adult (AD) were retrogradely labelled with cholera toxin horseradish peroxidase conjugate (CT-HRP).
     以CT-HRP逆行标记出生后一天(P_1)至成体(AD)的99只大鼠胫前肌运动神经元(TaMn)。
短句来源
  相似匹配句对
     Horseradish
     辣根
短句来源
     Determination of Horseradish Peroxidase by Spectrophotometry
     3,3′,5,5′-四甲基联苯胺-H_2O_2-HRP分光光度法测定HRP的研究
短句来源
     Progress of the Mimetic Enzyme of Horseradish Peroxidase
     辣根过氧化物酶模拟酶研究进展
短句来源
     A QUANTITATIVE STUDY OF HORSERADISH PEROXIDASE CONJUGATES
     辣根过氧化物酶标记抗体的定量研究
短句来源
     EVALUATION OF THE QUALITY OF HORSERADISH PEROXIDASE(HRP) CONJUGATES
     辣根过氧化物酶结合物的质量鉴定
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  horseradish peroxidase conjugate
Acetone-fixed whole Babesia bigemina-infected erythrocytes on micro-slides were immunoreacted with bovine serum samples followed by antibovine horseradish peroxidase conjugate and developed using diaminobenzidine tetrahydrochloride as a substrate.
      
Chicken anti-harp-seal immunoglobulin horseradish peroxidase conjugate served as the immunoconjugate.
      
The test is performed as a solid phase direct competitive ELISA using a horseradish peroxidase conjugate as the competing, measurable entity.
      
The test is performed as a solid phase direct competitive ELISA using a horseradish peroxidase conjugate as the competing, measurable entity.
      
Biotin-labeled rye DNA was detected using streptavidin-horseradish peroxidase conjugate.
      
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An enzyme-linked immunosorbent assay (ELISA) for the quantitation of human alpha-fetoprotein is described. This new technique provides a relatively simple, sensitive and reproducible means of the sandwich wethod. Only inexpensive equipment is used as compared with that of RIA. However, sensitivity threshold and reproducibility are within a range comparable to that of RIA. As principal reagent, peroxidase-labeled anti-human alpha-fetoprotein antibodies were prepared by periodate oxidized horseradish...

An enzyme-linked immunosorbent assay (ELISA) for the quantitation of human alpha-fetoprotein is described. This new technique provides a relatively simple, sensitive and reproducible means of the sandwich wethod. Only inexpensive equipment is used as compared with that of RIA. However, sensitivity threshold and reproducibility are within a range comparable to that of RIA. As principal reagent, peroxidase-labeled anti-human alpha-fetoprotein antibodies were prepared by periodate oxidized horseradish peroxidase conjugating to an IgG fraction of horse anti alpha-fetoprotein. purification of the conjugate was carried out on Sephadex G-200 column chromatography. A straight curve in the range 12.5-100ng/ml has been obtained. The coefficient of variation of two different concentration samples were 6.3% and 6.8% respectively. The dose-response curve covers a 100-fold range of analytic concentrations. The recovery rate of added antigen was 100.6±5.8%. Results could be obtained within 6 h. Determination results of AFP in 129 clinical samples accord with clinical diagnoses. When alpha-fetoprotein was quantitated in 20 sera both by radioimmunoassay(RIA) and enzyme-linked immunosorbent assay (ELISA), a Correlation coefficient of 0.95 was obtained between the two methods.

本文介绍了一种新的临床甲胎蛋白定量的ELIsA方法。此法操作简单、省时间、成本低廉,其灵敏度可以达到RIA水平。适合医院做为常规检查项目。辣根过氧化物酶用过碘酸钠法与抗甲胎蛋白IgG结合。酶标抗体是用SephadexG—200纯化的。标准曲线重现性良好,精密度实验批内变异系数平均为6.5%;回收率为100.6±5.8%;本文在缩短ELISA测定时间上做了改进,提出采用40℃包被和孵育,可在6小时内得到结果。本法与放射火箭电泳自显影法比较20例两法结果的相关系数r=0.95。129例血清甲胎蛋白ELISA测定,结果符合临床诊断。 (本研究标记部分工作在生物系生殖免疫研究室完成,得到刘学高副教授热情帮助和指导;临床部分工作在空军广州医院检验科完成,得到陈汉军、邸红同志大力支持和帮助;本文完成后,请第一军医大学生化室徐铃教授和白求恩大学生化室麦荫乔教授审阅。并提出宝贵意见,特此一并致谢!)

A specific sensitive and rapid ELISA (double antibody sandwich technique) with anti-human IgG and corresponding horseradish peroxidase conjugate was described for the quantitative determination of trace amounts of IgG. The chequerboard titrations were applied tor selection of optimal concentration of coating antibody and conjugate, they are 10μUg/ml and 1 : 2000 respectively. The sensitivity can reach as low as 3.0 ng/ml. It is suitable for research work as well as clinical study.Several factors...

A specific sensitive and rapid ELISA (double antibody sandwich technique) with anti-human IgG and corresponding horseradish peroxidase conjugate was described for the quantitative determination of trace amounts of IgG. The chequerboard titrations were applied tor selection of optimal concentration of coating antibody and conjugate, they are 10μUg/ml and 1 : 2000 respectively. The sensitivity can reach as low as 3.0 ng/ml. It is suitable for research work as well as clinical study.Several factors affected the method have been investigated. Reproducibility and specificity of the method were identified.

本文报道了一种特异、敏感,快速的酶联免疫吸附试验(ELISA)双抗体夹心法和抗人IgG及相应辣根过氧化物酶结合物用于测定微量IgG。用棋盘滴定法选择包被抗体和结合物的最适浓度分别为10μg/ml和1:2000,敏感性可达3.0ng/ml,适用于科研和临床研究。并探讨了影响本法的因素,确定了本法的重复性和特异性。

β-endorphin-immunoreactive neurons in the rat brain were localized by using the horseradish peroxidase conjugated staphylococcal protein A(HRP -PA), Brown orange β-endorphin-immunoreactive product in perikarya in the hypothalamic arcuate nucleus and in the pituitary were found. There were dense β-endorphin-immunoreactive granules in the ventral periaqueductal gray. The results show that HRP-PA is a valuable method for the locali-zation of β-endorphin-immunoreactive neurons in the rat brain.

本实验使用辣根过氧化物酶标记葡萄球菌蛋白A(HRP-PA)对大鼠脑内β-内啡肽(β-EP)免疫反应细胞进行定位。下丘脑弓状核和垂体的核周质中观察到棕黄色的β-EP免疫反应产物,在导水管腹侧有较密集的β-EP免疫反应颗粒。这结果表明,采用HRP-PA定位脑内β-EP免疫反应细胞乃是一种良好的方法。

 
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