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lectin binding proteins
相关语句
  凝集素结合蛋白
     Research on Artocarpus Lingnanensis Lectin Binding Proteins of Hu-man Serum or Colostrum
     人血清及初乳中红桂木凝集素结合蛋白的研究
短句来源
     Research on artocarpus lingnanensis lectin binding proteins in human urine and its clinical significance.
     尿液中红桂木凝集素结合蛋白的检测及临床意义
短句来源
  相似匹配句对
     THE CARBOHYDRATE BINDING SPECIFICITY OF PINELLIA LECTIN
     半夏凝集素的糖类结合专一性
短句来源
     Sugar Binding Activity of Lectin Fragments
     凝集素碎片的糖结合活性
短句来源
     protein binding;
     蛋白结合;
短句来源
     Research on artocarpus lingnanensis lectin binding proteins in human urine and its clinical significance.
     尿液中红桂木凝集素结合蛋白的检测及临床意义
短句来源
     Research on Artocarpus Lingnanensis Lectin Binding Proteins of Hu-man Serum or Colostrum
     人血清及初乳中红桂木凝集素结合蛋白的研究
短句来源
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Artocarpus lingnanensis lectin (ALL) was purified and studied on its physicochemical and binding immunoglobulin property.ALL had been extracted with pH 7 2,0 01 mol/L PBS,then isolated and purified from the seeds of Artocarpus lingnanensis by 30%~60% saturated ammonium sulfate fractionation and affinity chromatography on Gal Sepharose 6B eluted with 0 2 mol/L Gal.ALL is homogeneous basically on uncontinuous PAGE.The lectin,which was analysed by Sephadex G 100 chromatography and SDS...

Artocarpus lingnanensis lectin (ALL) was purified and studied on its physicochemical and binding immunoglobulin property.ALL had been extracted with pH 7 2,0 01 mol/L PBS,then isolated and purified from the seeds of Artocarpus lingnanensis by 30%~60% saturated ammonium sulfate fractionation and affinity chromatography on Gal Sepharose 6B eluted with 0 2 mol/L Gal.ALL is homogeneous basically on uncontinuous PAGE.The lectin,which was analysed by Sephadex G 100 chromatography and SDS PAGE,has an apparent M r of about 59 000 and is composed of two kinds of subunits which have M r 14 000 and 16 000 respectively.Isoelectric focusing on PAGE showed that lectin gave a spread of components,whose p I are 9 30,9 10,8 90,8 75,8 20 and 6 50 respectively.ALL is a glycoprotein with assay of schiff's stain.The two N terminals are arginine and glycine using DNS Cl method.It contains about 7.0% neutral sugar.ALL agglutinates erythrocytes of the animals and those of human's A,B,O and AB bloodgroups.The hemagglutination of ALL is strongly inhibited by Gal NAc and Gal in test of hemagglutinating inhibition.The lectin is sensitive to heat,but within the range of 4 5~9 5,stable to pH changes.By double immunodiffusion,it has been found that serum lectin binding protein could combine with goat anti human IgA and anti human IgG,colostrum lectin binding protein could combine with goat anti human IgA,These suggest that ALL can bind human serum IgA,IgG(mainly IgA)or colostrum IgA.The subspecies of Ig which ALL can bind will being studied.

红桂木(Artocarpuslingnanensis)、俗名胭脂,属桑科桂木属,为亚热带、热带植物.红桂木种子含丰富的红桂木凝集素(Artocarpuslingnanensislectin,ALL),但迄今国内外均未见关于它的报道.我们采用Gal-S...

Objective\ ALL HRP Sandwich assay was developed for the measurement of Artocarpus lingnanensis lectin binding proteins in human urine(U LBP) Methods \ ALL HRP was prepared and purified by Sephadex G 200 chromatography The optimum concentration of coating ALL and the working dilution degree of ALL HRP were determined for the measurement of U LBP With Sandwich enzyme linked assay intra and inter run precision studies were performed The standard curve was prepared for U LBP Results\ The working...

Objective\ ALL HRP Sandwich assay was developed for the measurement of Artocarpus lingnanensis lectin binding proteins in human urine(U LBP) Methods \ ALL HRP was prepared and purified by Sephadex G 200 chromatography The optimum concentration of coating ALL and the working dilution degree of ALL HRP were determined for the measurement of U LBP With Sandwich enzyme linked assay intra and inter run precision studies were performed The standard curve was prepared for U LBP Results\ The working concentrafion range of the standard curve was 9 4~150 μg/ml for U LBP The value of U LBP/Cr(mg/mmol)in normal human urine or patient′s urine with SLE nephropathy,acute or chronic glomerulonephritis(AGN or CGN),nephrotic syndrome,hematuria,pyelonephritis,diabetes mellitus,were determined with ALL HRP Sandwich enzyme linked assay respectively The value of U LBP/Cr(mg/mmol)in normal human urine was 3 44±1 56 mg/mmol,the value of U LBP/Cr(mg/mmol)in SLE nephropathy patient urine,44 06±32 47 mg/mmol was highest;secondly,AGN,38 07±26 13 mg/mmol;nephrotic syndrome 32 57±20 33 mg/mmol;hematuria 25 30±14 62 mg/mmol;pyelonephritis 17 37±11 77 mg/mmol;CGN 18 03±13 70 mg/mmol;diabetes mellitus 15 69±12 08 mg/mmol;respectively The comparison of the value of U LBP/Cr between patients and normal human was distinctly differential Intra assay CV 8 96%,inter assay CV 9 89% Conclusion\ The measurement on U LBP in urine by Sandwich enzyme linked assay with ALL HRP is simple and precise,can be used in wide range The assay on U LBP in urine is important for the diagnosis on some kidney diseases

目的 建立酶标记红桂木凝集素 (ALL HRP)夹心法检测尿液中ALL结合蛋白。方法 制备ALL HRP并经SephadexG 2 0 0纯化作为酶标凝集素 ,ALL为包被物 ,确定最适包被浓度及ALL HRP工作稀释度 ,夹心酶联检测尿液中ALL结合蛋白 (U LBP)。经精密度、重现性等实验并制备标准曲线。结果 以U LBP作标准制备的标准曲线线性检测范围为 9 4~ 15 0 μg/ml。本法测定几种疾病的尿U LBP/Cr( μg/ μmol) ,以SLE肾病为最高 4 4 0 6± 3 2 4 7,其次是急性肾炎 3 8 0 7± 2 6 13 ,肾病综合征 3 2 5 7± 2 0 3 3 ,血尿 2 5 3 0± 14 62 ,慢性肾炎 18 0 3± 13 70 ,糖尿病 15 69±12 0 8,与正常对照组相比 ,均有显著性差异。本法批内CV为 8 86% ,批间CV为 9 89%。结论 ALL HRP夹心酶联法检测U LBP方法简便 ,结果稳定 ,可望在临床上推广使用。

 
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