助手标题  
全文文献 工具书 数字 学术定义 翻译助手 学术趋势 更多
查询帮助
意见反馈
   therapeutic genes 的翻译结果: 查询用时:0.01秒
图标索引 在分类学科中查询
所有学科
肿瘤学
更多类别查询

图标索引 历史查询
 

therapeutic genes
相关语句
  治疗基因
     Gene therapy provides new hopes to cure diseases by delivering therapeutic genes to certain location or cells or by replacing and repairing dysfunctional genes.
     基因治疗( gene therapy )通过输送治疗基因到特定的位置或细胞,从而表达有治疗功能的蛋白或者直接取代、修复有问题的基因。 这种新的治疗方式为严重疾病的治愈带来了希望。
短句来源
     CONCLUSION: Cotransduction of triple therapeutic genes with separate AAV vectors may be a feasible approach to the clinical application of gene therapy for Parkinson's disease.
     结论:不同种类的AAV载体介导的3种治疗基因共转导对帕金森病基因治疗的临床应用将可能是一种切实可行的有效途径。
短句来源
     A series of problems were met in the procedure of delivering tumor therapeutic genes into cells using cationic polymers as vectors,current studies have provided sorts of solutions and greatly developed the practical characters of cationic polymers,and confer good understanding on these vectors.
     采用阳离子载体将肿瘤治疗基因送入靶细胞中表达 ,须经历体内传递、穿过细胞膜、细胞内传递、细胞内表达等多个步骤。 当前研究表明 ,阳离子载体在此过程中面临一系列独特的问题 ,对这些问题的研究与解决大大推动了对阳离子载体的认识与应用。
短句来源
     Conclusion The toxin genes, working as therapeutic genes, are suitable for the tissuesspecific gene therapy for colorectal carcinoma.
     结论 ADP核糖化毒性基因作为治疗基因适合于肿瘤组织特异性基因治疗
短句来源
     The main difficulty in its practice is how to make the therapeutic genes transfect into the human body efficiently and safely and express endurably, stably and efficiently.
     如何将肿瘤治疗基因安全高效的转移到人体内,使治疗基因在人体内长期、稳定、高效的表达是当前基因治疗实践中所遇到的重大困难。
短句来源
更多       
  “therapeutic genes”译为未确定词的双语例句
     Adenoviral-mediated intracavernosal transfer of therapeutic genes and stem cells thearapy,such as eNOS and RhoA/Rhokinase and mesenchymal stem cell-based cell,were performed.
     基因和干细胞(eNOS、RhoA/Rho-kinase与Mesenchymal stem cell-based cell)治疗糖尿病性ED取得一些进展。
短句来源
     In summary, the MDR1 gene can be efficiently expressed under control of IRES in a cap-independent manner, and it may be useful for gene therapy in two ways: to protect stem/progenitor cells from myelosuppression after chemotherapy in patients with cancer and to act as a dominant selectable marker in bicistronic vectors in vivo for the coexpression with therapeutic genes.
     因而,MDR1基因可在IRES控制下以帽非依赖性方式进行有效表达,其在基因治疗研究中可有两种用途:在癌症患者化疗后用于保护干/祖细胞,或在双顺反子载体中作为体内显性选择性标志用于共表达治疗性基因。
短句来源
     Objectives: Adeno-associated virus was applied to mediate hTGF-β1 and hTGF-β3 encoding genes to the degenerative nucleus pulposus cells of rabbits, the expression and biological effects on matrix repair of therapeutic genes were assessed.
     目的:制造兔椎间盘退变模型; 应用腺相关病毒(adeno-associated virus,AAV)介导人转化生长因子β1(human transforming growth factor-β1,hTGF-β1)、hTGF-β3基因体内转染兔退变椎间盘,观察目的基因的表达以及单基因转染或双基因联合转染对退变髓核细胞的生物学作用。
短句来源
     Great deals of researches have indicated that interleukin 2 and interleukin 12 genes would be potentially therapeutic genes, which bring into full play by activating natural killer cell (NK) and cytotoxic T lymphocyte (CTL).
     大量研究表明,细胞因子IL-2和IL-12具有较好的抗瘤效应,两者通过激活自然杀伤细胞(NK)和细胞毒T淋巴细胞(CTL)发挥细胞免疫功能。
短句来源
     p27 could be act as a kind of therapeutic genes for lung gene therapy.
     用基因传递载体携带p27基因治疗小鼠肺部肿瘤,国内外尚未见报道。
短句来源
更多       
  相似匹配句对
     cytoskeleton genes ;
     细胞骨架相关基因;
短句来源
     Therapeutic evaluation:
     一、疗效评价:
短句来源
     RADIOSENSITIVITY AND GENES
     辐射敏感性和基因(英文)
短句来源
     Progress of Treatment of Therapeutic Angiogenesis Genes in Ischemic Heart Disease
     治疗性血管新生基因治疗缺血性心脏病的研究进展
短句来源
     Therapeutic Cloning
     治疗性克隆
短句来源
查询“therapeutic genes”译词为用户自定义的双语例句

    我想查看译文中含有:的双语例句
例句
为了更好的帮助您理解掌握查询词或其译词在地道英语中的实际用法,我们为您准备了出自英文原文的大量英语例句,供您参考。
  therapeutic genes
Vectors derived from the adeno-associated viruses (AAV) have been successfully used for the long-term expression of therapeutic genes in animal models and in patients.
      
Gene delivery systems are designed to control the location of administered therapeutic genes within a patient's body.
      
They have been engineered to function as vectors for delivering therapeutic genes in gene therapy strategies, and as direct cytotoxic agents in oncolytic viral therapies.
      
Since most acquired diseases are not caused by single gene mutations, the choice of therapeutic genes is crucial for the success of the gene therapy.
      
Understanding the mechanisms of the inactivation and reactivation of CMV promoter-controlled transgenes should help develop an effective strategy to fully activate the CMV promoter-controlled therapeutic genes silenced by the host cells.
      
更多          


Retroviral vectors are the most used gene delivery vehicle in human gene therapy.When the therapeutic gene is packagedfrom producer cell line, one has to determined whether thereplication-competent retrovirus(RCR) is being generated. In this study, two methods, namely the marker rescue assay and RT/PCR, were employed to detect RCR in the cells. While the marker rescue assay can detect RCR with a limit at 6×102CFU/ml, RT/PCR can be used to detect RCR at as low as lCFU/103ml. Combining the specificity...

Retroviral vectors are the most used gene delivery vehicle in human gene therapy.When the therapeutic gene is packagedfrom producer cell line, one has to determined whether thereplication-competent retrovirus(RCR) is being generated. In this study, two methods, namely the marker rescue assay and RT/PCR, were employed to detect RCR in the cells. While the marker rescue assay can detect RCR with a limit at 6×102CFU/ml, RT/PCR can be used to detect RCR at as low as lCFU/103ml. Combining the specificity of the marker rescue assay and the sensitivity of RT/PCR, both assays together should serve as an adequate test for detecting the generation of RCR in retroviral producer cell lines.

运用逆转录病毒作为外源基因导入的载体,在实际临床运用时需要检测包装细胞在表达目的基因的同时,是否会产生有复制能力的逆转录病毒。本实验运用了Marker Rescue Assay(补救分析)和RT/PCR(逆转录PCR)两种方法。补救分析可检测到6×10~2CFU/ml(Colony Forming Units/ml)的有复制能力的逆转录病毒,RT/PCR的灵敏度为1CFU/10~3ml。这两种检测方法的建立,为逆转录病毒载体用于临床基因治疗的安全性提供一定的保证。

Based on a vector, pSSV9-int,which bearing the genome of wild type adeno-associ-ated virus type-2, a universal recombinant AAV vector pSSV9/MulV-neosv was constructed. It was used for cloning and expressing enkaryotic genes.The neo gene in the vector was expressed. Further-MulV-neosv-IL-2, which bears a therapeutic gene,human interleukin-2, was constructed. After trans-fecting A549 cells with the plasmid, the cells pro-duced IL-2. This work provided a foundation for the further research in...

Based on a vector, pSSV9-int,which bearing the genome of wild type adeno-associ-ated virus type-2, a universal recombinant AAV vector pSSV9/MulV-neosv was constructed. It was used for cloning and expressing enkaryotic genes.The neo gene in the vector was expressed. Further-MulV-neosv-IL-2, which bears a therapeutic gene,human interleukin-2, was constructed. After trans-fecting A549 cells with the plasmid, the cells pro-duced IL-2. This work provided a foundation for the further research in human gene therapy using the rAAV vectors-

以野生型2型人腺伴随病毒(AAV-2)基因组载体pSSV9-int-为基础,构建了一种携带和表达真核基因的基础AAV载体pSSV9/MulV-neosv,表达了neo基因,并采用该载体组建了携带人白细胞介素-2cDNA的重组AAV载体pSSV9/MulV-neosv-IL-2,转染A549细胞后表达了人白细胞介素-2基因。为应用腺伴随病毒作基因治疗研究提供了实验基础。

Objectives\ To evaluate the quality and quantity of human umbilial cord blood hematopoietic stem cells for transplantation,the percentage, abilities of proliferation and differentiation,expansion responses and grafting efficiency of CD34 + cells and their subpopulations from umbilical cord blood were studied.Methods CD34 + cells and their subsets (CD34 +CD38 + and CD34 +CD38 -) were isolated from human umbilical cord blood by using MACS or FACS system,cultured and expanded with hematopoietic growth factors (HGFs)...

Objectives\ To evaluate the quality and quantity of human umbilial cord blood hematopoietic stem cells for transplantation,the percentage, abilities of proliferation and differentiation,expansion responses and grafting efficiency of CD34 + cells and their subpopulations from umbilical cord blood were studied.Methods CD34 + cells and their subsets (CD34 +CD38 + and CD34 +CD38 -) were isolated from human umbilical cord blood by using MACS or FACS system,cultured and expanded with hematopoietic growth factors (HGFs) in semi solid and liquid culture systems, and the grafting efficiency was evaluated by Panning assay. Results\ The percentage of CD34 +CD38 - subset in cord blood was much higher (9 5%) than that in bone marrow or peripheral blood (4%) ( P<0 01 ). More hematopoietic colony formation from cord blood than that from bone marrow or peripheral blood was observed. With addition of HGFs, the total uncleated cells increased by 500.0±82.5-fold, the hematopoietic progenitor cells by 94.1±28.6-fold and the CD34 + cells by 24.0±3.8-fold. Only 36% of CD34 + cells were taken in a pre formed adult bone marrow stroma in a short time (2hr), but 68%-89.6% of those pretreated with GM CSF and / or IL 3 for 6-8hr were taken in the stroma ( P<0 01 ). Conclusions\ It is possible to expand cord blood CD34 + cells ex vivo to improve their grafting efficiency for utilization of cord blood stem cells in treatment of acute radiation sickness, transplantation and therapeutic gene transfer.

目的对脐带血造血干细胞移植的“质”“量”作出评估,使其更广泛有效地应用于临床。方法利用免疫磁珠分离法、FACS分析与分选、体外液体培养、铺展贴壁(Panning)等方法对脐带血CD34+造血干、祖细胞及其的数量、体外增殖分化性能、生长因子扩增效应、植入成人骨髓基质效率等进行研究,数据经t检验。结果脐带血有核细胞、CFCs、CD34+细胞及其亚群(CD34+CD38+和CD34+CD38-)等的绝对数量明显低于常规骨髓移植所需的细胞数量,但脐带血CD34+CD38-亚群占CD34+细胞的比例(9.5%)明显高于骨髓或外周血(4%)(P<0.01)。脐带血CD34+细胞及其亚群的增殖分化能力明显高于骨髓和外周血,可形成更多的各系造血集落和维持更长久的造血活动。且对造血生长因子具有良好的扩增效应,细胞总数、CFCs及CD34+细胞可分别被扩增500.0±82.5倍、94.1±28.6倍和24.0±3.8倍,但扩增也加速了细胞的分化。此外,仅有36%的脐带血CD34+细胞可在短时间内(2小时)植入成人骨髓基质,但经GM-CSF和/或IL-3预处理6~8小时后,可明显提高其移植效率,植入成人骨髓基质的CD34+细胞达?

 
<< 更多相关文摘    
图标索引 相关查询

 


 
CNKI小工具
在英文学术搜索中查有关therapeutic genes的内容
在知识搜索中查有关therapeutic genes的内容
在数字搜索中查有关therapeutic genes的内容
在概念知识元中查有关therapeutic genes的内容
在学术趋势中查有关therapeutic genes的内容
 
 

CNKI主页设CNKI翻译助手为主页 | 收藏CNKI翻译助手 | 广告服务 | 英文学术搜索
版权图标  2008 CNKI-中国知网
京ICP证040431号 互联网出版许可证 新出网证(京)字008号
北京市公安局海淀分局 备案号:110 1081725
版权图标 2008中国知网(cnki) 中国学术期刊(光盘版)电子杂志社