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genes dna
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  基因dna
     Construction and Expression of IBDV VP2/VP243 Genes DNA Vaccine Expressing Plasmids
     IBDV VP2/VP243基因DNA疫苗表达质粒的构建与表达
短句来源
     Differential expression genes and ESTs were 20 with signal log ratio ≥1 and 70 with signal log ratio ≤-1, mainly included ion channel and transport protein relative genes, cell apoptosis and stress reaction protein relative genes, metabolism relative genes, cell signal transduction and transferrin relative genes, DNA’s binding, transcription and transcriptional factor relative genes, immunological and inflammatory reaction relative genes, and others, in the Heart meridian group vs the model group.
     心经组中有70个下调和20个上调,主要是离子通道和运输蛋白相关基因,细胞凋亡和应激反应蛋白相关基因,代谢相关基因,细胞信号和传递蛋白相关基因,DNA结合、转录和转录因子类基因,免疫和炎性反应相关基因等。
短句来源
     Conclusion The regulation of genes including stress response genes,immune related genes,DNA synthesis and repairing genes,metabolic genes may be involved in antitransforming activity of Chlorophylin.
     结论 叶绿酸的抗转化活性与应激反应基因 ,免疫相关基因 ,DNA合成和修复基因 ,代谢相关基因等多类基因的调节有关
短句来源
     They mainly included some Ion meridianand transport protein relative genes,metabolism relative genes,cell signal transduction and transferin relative genes,DNA's binding,transcription and transcriptional factor relative genes,immumological and inflammatory reaction relative genes,et al.
     小肠经组差异表达大于2倍的基因主要是离子通道和运输蛋白相关基因,代谢相关基因,细胞信号和传递蛋白相关基因,DNA结合、转录和转录因子类基因,免疫和炎性反应相关基因等。
短句来源
     Conclusion The regulation of genes including tumor suppressor gene,apoptosis and stress response genes, immune related genes, DNA synthesis and repair genes, metabolism genes may be involved in transforming activity of NiS and BPDE. cDNA microarray technique is effective in screening the differentially expressed genes among different kinds of xenobiotics.
     结论 NiS与BPDE对 16HBE细胞株的转化作用在抑癌基因 ,细胞周期蛋白相关基因 ,细胞凋亡和应激反应基因相关基因 ,DNA合成、修复和重组蛋白相关基因 ,DNA结合、转录和转录因子类基因 ,细胞受体相关基因 ,代谢相关基因 ,蛋白翻译和合成相关基因等多类基因上呈现差异表达。
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  “genes dna”译为未确定词的双语例句
     Construction and Expression of NDV HN/F genes DNA Vaccine Expresssion Plasmids
     NDV长春株和四平株HN/F核酸疫苗的构建及表达
短句来源
     Based on the progress reported in this field, we selected three genes(LGI1, CHRNA4, CHRNB2) as candidate genes, DNA sequencing was performed on all their exons and the exon-intron splicing sites.
     检索国外的研究进展,选取候选基因(LGI1、CHRNA4、CHRNB2),进行突变筛查研究。
短句来源
     The differentially expressed genes include the genes related to metabolism enzymes, immune response-related genes, DNA damage repair genes, stress response genes and membrane proteins. Phase I enzyme p450IIC13 and phase II enzymes GSTA2, SULTLE2 were up-regulated. IL1B, HSPB1 were down-regulated.
     诱导I相代谢酶P450IIC13和II相代谢酶GSTA2、SULTLE2和促进DNA修复GADD45A基因表达上调,部分代谢酶、免疫、应激有关基因表达下调。
短句来源
     The differentially expressed genes include metabolism enzymes and immune response-related genes, DNA damage repair genes, stress response genes and genes of membrane proteins. Phase Ⅰ enzyme p450 2C13 and phase Ⅱ enzymes GSTA2,SULTLE2 were up-regulated. IL1B,HSPB1 were down-regulated.
     诱导Ⅰ相代谢酶P45011C13和Ⅱ相代谢酶GSTA2、SULTLE2和促进DNA修复GADD45A基因表达上调,部分代谢酶、免疫、应激有关基因表达下调。
     The CpG island methylation in promoter region of tumor suppressor genes (TSG) , cell cycle controlling genes, DNA repair genes and tumor invasion related genes is closely correlated with the development of glioma.
     大量肿瘤抑制基因、细胞周期调控基因、DNA损伤修复基因、肿瘤侵袭相关基因等启动子区域CpG岛甲基化,与胶质瘤的发生发展密切相关。
短句来源
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  相似匹配句对
     The DNA Sequencing of CMV Replicase Genes
     CMV复制酶基因的克隆及序列分析
短句来源
     Genes are fragments of DNA sequences with hereditary information.
     基因是具有遗传效应的DNA片段,它控制着生物体的遗传性状。
短句来源
     DNA Vaccine
     DNA疫苗
短句来源
     DNA BIOSENSOR
     DNA生物传感器
短句来源
     ? genes were overexpressed in E.
     亚基及其突变体能在E.
短句来源
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  genes dna
Ferritin genes (DNA and RNA) and protein function at the intersection of iron and oxygen chemistry in biology.
      
Screening for mutations in the MODY genes DNA was isolated from peripheral leucocytes.
      
In several regulatory sequences of developmental genes DNA binding sites for regulatory transcription factors are overlapping.
      


A broad host range osm plasmid pJG137 was coustructed by molecular cloning of DNA. Genetic expression indicated that this plasmid is a subcloued product of proBA osm gene DNA of plasmid pLA101 and DNA of the broad host range plasmid pRK290, by four different approaches: 1) minisereen of DNA, 2) restriction mapping of DNA, 3) Southern transfer of DNA, and 4) antibiotic and toxin tolerance.

宿主范围广的调渗质粒pJG137用DNA分子克隆方法建成。基因表达表明:这一质粒是由pLA101质粒的proBA调渗基因DNA,与宿主范围广的pRK290质粒DNA克隆而成的产物。这从以下四方面加以表明:1)DNA的微型筛选;2)DNA的限制性内切酶谱;3)Southern氏DNA分子杂交,4)对抗生素和毒素的抗性。

Plasmid pRBα1 and vectors M13 mp7 ,M I3mp8 were cut by PstI.The 1.5kb-PstI fragment including intact human α1-globin gene and M]3mp7 or M13mp8 was recombinated.By means of dot hybridization technique, two clones carrying human α-globin gene DNA were selected and named M13mp7α1, M13mp8 α1 respectively.Their SS-DNA, RF-DNA and PstI-cleaved RF-DNA samples were separated by electrophoresis in agarose gels.It was confirmed that these two recombinants contained the 1.5kb-PstI fragment.Plasmid...

Plasmid pRBα1 and vectors M13 mp7 ,M I3mp8 were cut by PstI.The 1.5kb-PstI fragment including intact human α1-globin gene and M]3mp7 or M13mp8 was recombinated.By means of dot hybridization technique, two clones carrying human α-globin gene DNA were selected and named M13mp7α1, M13mp8 α1 respectively.Their SS-DNA, RF-DNA and PstI-cleaved RF-DNA samples were separated by electrophoresis in agarose gels.It was confirmed that these two recombinants contained the 1.5kb-PstI fragment.Plasmid pUR222DNA digested with EcoRI and PstI simultaneously was used as primer. M 1 3mp3α1 SS-DNA were used as template. The (-) strand of the template DNA can be synthesized and labeled with α32P-dNTP, but the synthesis did not proceed to completion under our experimental conditions. Therefore, the inserted sequence was kept single stranded.Thus, a partially double stranded probe, having specificity of globin gene and high specific activity, usually of 5-7×108dpm/μg gene fragment, was obtained.

利用人完整α_1-珠蛋白基因的1.5kbDNA片段,与载体M13mp7,M13mp8DNA重组,得到两株含人α_1-珠蛋白基因的克隆7~#及2~(’#),并为其RF-DNA的限制性内切酶图谱分析所证实。以2_(’#)SS-DNA为模板,以EcoRI和PstI双酶切质粒pUR222直接变性作引物,以α-32P-dATP作放射性标记,合成载体M13正链的放射性互补链;而插入的α_1-珠蛋白基因部分仍呈单链。α_1-珠蛋白基因单链特异性的探针其比活度通常可达5~7×10~8dpm/μg基因片段。

Experiments of cloning and expressing sequences coding for a nonstructural protein gene NSI from the long leftward open reading frame of the autonomous parvovirus FPV by using bacteriophage λgtll were conducted.These experiments composed of preparing the target gene DNA, extracting the λgtll DNA, Iigating the target gene DNA with λgtll DNA, in vitro packaging, screening the phage plagues and lysogens, detecting the recombinant gene products.Fusion proteins expressel by the...

Experiments of cloning and expressing sequences coding for a nonstructural protein gene NSI from the long leftward open reading frame of the autonomous parvovirus FPV by using bacteriophage λgtll were conducted.These experiments composed of preparing the target gene DNA, extracting the λgtll DNA, Iigating the target gene DNA with λgtll DNA, in vitro packaging, screening the phage plagues and lysogens, detecting the recombinant gene products.Fusion proteins expressel by the recombinant gene were thus obtaincd.These fusion proteins were specifically bound by both antisera raised against one of the fusion proteins and antisera from a canine parvovirus(CPV) in fected dog.The results obtained showed that the bacteriophage λgtll is a good vector for gene manipulation and its extending use will open vast vistas in serodiagnosis and vaccine prevention.

大肠杆菌噬菌体λgt11是在原有基础上经不断改进而组建成的一个新的基因载体,通过一系列检测方法的建立,已成为一个完整的基因操作系统。本文应用此系统对细小病毒的非结构蛋白基因NSI进行了克隆和表达试验,包括目的基因的制备、载体DNA的提取、基因拼接、噬菌体的体外包装、重组噬菌体蚀斑和溶原菌的抗体探子筛选以及重组体基因产物的检测,最后获得了由重组体基因表达产生的融合蛋白质。此蛋白质能被兔源抗NSI抗体和自然感染细小病毒的犬血清特()性地结合。试验结果表明,λgt11是基因操作的一个有效的载体系统,它在疫病的血清学诊断和疫病预防的研究上具有广阔的应用前景。

 
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