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plasmid genes
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  “plasmid genes”译为未确定词的双语例句
     Overexpression and purification of two oligopeptide binding proteins OppA4 and OppA5 encoded by plasmid genes of Borrelia burgdorfei
     两种质粒编码的Borrelia burgdorfei寡肽结合蛋白(OppA)的表达和纯化
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     Protein expression was characterized by the method of labeling and electrophoresis, since the addition of rifampin and 35S-Met together with IPTG blocks the expression of the Escherichia coli genome and only synthesis of the plasmid genes was detected. The result showed that special bands did exist in the cell lysate transformed with interest clone.
     将上述3种聚合体基因分别克隆大肠杆菌(Escherichia coli)表达载体pET-30a(+),经IPTG诱导、利福平阻断菌体基因表达及在培养基中添加35S-M et,通过放射自显影可见由T7启动子引发的特异目的基因表达带。
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  相似匹配句对
     Researches of Agrobacterium rhizogenes Ri Plasmid rol Genes
     发根农杆菌Ri质粒rol基因的研究现状(英文)
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     A PLASMID WITH GENES CODING FOR BENZENE HEXACHLORIDE DEGRADATION
     BHC降解性质粒的研究
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     The recombinant plasmid of E.
     应用细菌质粒转化技术,将大肠杆菌K_(?)
短句来源
     cytoskeleton genes ;
     细胞骨架相关基因;
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     RADIOSENSITIVITY AND GENES
     辐射敏感性和基因(英文)
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  plasmid genes
The plasmid genes of these merodiploids are donated together to recipient cells and eliminated together from host cells both spontaneously and after treatment with acridine orange.
      
coli, of rickettsial chromosomal and plasmid genes encoding outer membrane proteins.
      
Plasmid genes involved in the interactions between bacteria and plant cells
      
(4) There was an excess of amino-acid replacement polymorphisms, relative to synonymous polymorphisms, in the plasmid genes, which is consistent with the hypothesis of diversifying selection among colicin-producing plasmid families.
      
(3) No characteristic differences were found in either the plasmid genes or the chromosomal gene between the 1974 isolates and the Murray strains, indicating that these plasmids have been stable for at least several decades.
      
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Plate (or slope) cultures of various derivatives of S. qingfengmyceticus showed spontaneously developing pocks which appeared frequently during crossing intra- or inter specifically. Strains which is able to produce pock (Ltz+) or which may be used as pock producing detector (Ltz-) can be isolated from the center or round spores of the pock, respectively.The results reported here indicate that the occurrence of pock is a lethal event of Ltz+ to Ltz- strain, and the lethal rate is up to above 99%. The genetic...

Plate (or slope) cultures of various derivatives of S. qingfengmyceticus showed spontaneously developing pocks which appeared frequently during crossing intra- or inter specifically. Strains which is able to produce pock (Ltz+) or which may be used as pock producing detector (Ltz-) can be isolated from the center or round spores of the pock, respectively.The results reported here indicate that the occurrence of pock is a lethal event of Ltz+ to Ltz- strain, and the lethal rate is up to above 99%. The genetic factor deter-ming this phenomenon possesses an infectious transfer property, and can be eliminated by treatment with acridine yellow, high temperature preincubation or through the course of protoplast regeneration. Therefore we consider that the pock formation ability in S. qingfengmyceticus may be determined by plasmid gene (or genes). The possible origin of such plasmid is discussed.

庆丰链霉菌各种衍生菌株的平板或斜面能自发产生麻点现象(pock),而当种内或种间杂交时,这种现象出现得更频繁。从麻点中心和周围孢子中可分别分离到具有产生麻点能力的菌株(Ltz~+)和本身虽然不能产生麻点但却可以作为检测麻点产生的指示菌株(Ltz~-)。 本文的研究结果表明,庆丰链霉菌的麻点现象是Ltz~+菌株和作为指示菌的Ltz~-菌株细胞之间在接合过程中的一种致死接合作用(lethal zygosis),致死率可达99%以上。决定这种致死能力的遗传因子能感染转移,可用已知质粒消除因子(如吖啶黄、高温预培养)处理及原生质体再生过程诱发消除。据此,我们认为庆丰链霉菌中产生麻点的能力很可能由质粒基因所决定。对于这种质粒的可能来源进行了讨论。

The gram-negative soil bacterium Agro-bacterium tumefaciens induces neoplasticgrowths called crown gall tumours on manydicotyledonous plants.Tumour formation isaccompanied by transfer and integration of aspecific part of the tumour inducing(Ti) plas-mid into the nuclei of the host cells.Thispart of the Ti plasmid is termed T region inthe bacteria and T-DNA in the plant cells.Crown gall tissues synthesize unusual T-DNAspecific compounds known as opines,the Tiplasmids are classified according to the typeof...

The gram-negative soil bacterium Agro-bacterium tumefaciens induces neoplasticgrowths called crown gall tumours on manydicotyledonous plants.Tumour formation isaccompanied by transfer and integration of aspecific part of the tumour inducing(Ti) plas-mid into the nuclei of the host cells.Thispart of the Ti plasmid is termed T region inthe bacteria and T-DNA in the plant cells.Crown gall tissues synthesize unusual T-DNAspecific compounds known as opines,the Tiplasmids are classified according to the typeof opine,the so called octopine and nopalineTi plasmid have been studied most extensively.Despite the fact that Ti plasmids are onlydistantly related,their T region share a highlyhomologous 9 Kb sequence involved in onco-genicity. At least three genes of the common DNAare directly involved in tumour induction.Thegene 4 codes for cytokinin synthese,it inhi-bits root formation and stimulates shoot for-mation in transformed plant tissues.The gene1 and gene 2 code for two enzymes involvedin auxin—IAA biosynthesis.From pGV 354;a clone carrying Hind Ⅲ 15—Hind Ⅲ 22 largefragment of pTiC 58 T-DNA,we subclonedthe gene 1 and gene 2 and constructed a inter-mediate vector pBZ 692 carrying a kanamycinresistance gene for screening in Agrdbacteriumtumefaciens. Using Ti plasmid gene vector pGV 3850which is a modified Ti plasmid,all onc genesof which are deleted and replaced by pBR 322sequence,we introduced gene 1 and gene 2into higher plants.The strain carrying gene 1and gene 2 can induce root formation on thestem of tobacco and potato and on the sun-flower hypocotyl.The transformed roots rgewon MS_0 medium and dedifferentiated to formcallus.The marker—nopaline was detected intransformed roots and callus.The effects ofgene 1 and gene 2 are just like the action ofauxin—IAA.Both gene 1 and gene 2 are ne-cessary for auxin independent growth,to sup-press shoot formation and to stimulate rootformation. In tissue and protoplast culture of mono-cotyledonous plant,it usually needs high levelof auxin to stimulate cell division.If gene 1and gene 2 transformed tissues or protoplastsare taken to culture,they may be able toregenerate into whole plants. Plant hormones control plant growth. development; cell division,differentiation andregulate transcription of genes.The oncgenes of Ti plasmid encode enzymes for pho-tohormone biosynthesis.Using onc genes,wecan study not only the regulation of expres-sion of foreign genes in transformed plants,but also the mechanism of cell differentiation.

根癌农杆菌Ti质粒的T区DNA带有致瘤基因,其基因1和基因2编码生长素吲哚乙酸生物合成途径中的两个酶。以pGV 354(pBR322质粒中插有Ti质粒C 58 T区DNA的HindⅢ15—HindⅢ22大片段)重组质粒出发,我们分离了基因1和基因2,并构建了带有卡那霉素抗性基因的重组质粒pBZ 692,通过基因载体pGV 3850,我们将基因1和基因2引入了高等植物。结果证明基因1和基因2能促使烟草、向日葵、土豆等转化组织分化长根,转化的根在MS_0培养基上能脱分化形成愈伤组织并自主生长,在转化的组织中有转化标记胭脂碱的存在。

The introduction and expression of TiT-DNA(transferred DNA on Ti plasmid)genes, harbored by strains B6S3 or T37 of Agrobacterium tumefaciens, into sunflower cells not only resulted in transformed cells capable of synthesizing special gene products but also enabled them to utilize D-lactose as a sole carbon source and be resisrant to some metabolic analogtles(e.g. BUdR). Moreover, a number of physiological and biochemical activites in host plan cells could also be affected by integrated TiT-DNA...

The introduction and expression of TiT-DNA(transferred DNA on Ti plasmid)genes, harbored by strains B6S3 or T37 of Agrobacterium tumefaciens, into sunflower cells not only resulted in transformed cells capable of synthesizing special gene products but also enabled them to utilize D-lactose as a sole carbon source and be resisrant to some metabolic analogtles(e.g. BUdR). Moreover, a number of physiological and biochemical activites in host plan cells could also be affected by integrated TiT-DNA genes. The peroxidase activity, isoperoxidase gene expression, protein composition and proportion, and free amino-acid pool were significantly different between transformed lines(both B6S3 lines and T37 lines)and normal lines of sunflower which growed on either hormone-containing media or hormorte-free media. The precursor aminoacids related to opine biosynthesis had a higher metabolic levels in these transformed lines.

根癌农杆菌B6S3和T37菌株Ti质粒T-DNA基因(TiT-DNA)在向日葵基因组中的导入与表达,不仅使转化细胞产生特殊的基因产物,并且使之能将D-乳糖作为唯一碳源利用和对某些代谢类似物(BUdR)具有抗性,此外还影响宿主细胞一系列生理生化活动。在无激素和含激素两种培养条件下,向日葵TiT-DNA转化系与相应正常系在过氧化物酶的活性及其同工酶基因的表达、蛋白质的种类及比例、游离氨基酸库等均存在着较大的差异。在转化系内,有关冠瘿碱生物合成的前休氨基酸具有较高的代谢水平。

 
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