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sodium periodate method
相关语句
  高碘酸钠法
     Studies on Immobilization of Lipase on Cellulose Membrane Using Sodium Periodate Method
     棉纤维膜上高碘酸钠法固定化脂肪酶
短句来源
     The anti-chlorpyrifos polyclonal antibodies were obtained by using the artificial immune antigen toimmune the New Zealand white rabbits. The enzyme tagged antibodies were prepared by coupling horseradish peroxidase(HRP) to the purified antibody with the modified sodium periodate method.
     将免疫抗原AR-BSA免疫兔子,制得了高效价的抗毒死蜱多克隆抗体,采用改良高碘酸钠法将纯化的抗体制备了酶标抗体。
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  “sodium periodate method”译为未确定词的双语例句
     Prepared anti-IBDV rabbit IgG and anti-chicken rabbit IgG were conjugated with horseradish peroxidase (HRP) by the reformative sodium periodate method and achieved preferable purpose. Their work concentrations were 1:1000 and 1:12000 respectively.
     采用改良过碘酸钠法将提纯的兔抗IBDV IgG和兔抗鸡IgG进行辣根过氧化物酶(HRP)标记,效果比较理想,二者工作浓度分别为1:1000,1:1200。
短句来源
     HRP-labelled estradiol antigen and antibody (E2-BSA-HRP and 1E3-HRP) were prepared by coupling the E2-BSA and 1E3 with HRP by the improved sodium periodate method. The optimised working concentrations of coated antigen and antibody were performed with phalanx titration.
     利用杂交瘤技术获得了两株稳定分泌E2单克隆抗体的杂交瘤细胞株1E3和1B4,经鉴定1E3细胞株分泌的单克隆抗体为IgG2b 亚型,1B4 细胞株分泌的单克隆抗体为IgG1 亚型;
短句来源
  相似匹配句对
     OXIDATIVE DETERMINATION OF INOSITOL WITH SODIUM PERIODATE
     高碘酸钠氧化法测定肌醇含量
短句来源
     Study on oxidation of cellulose to oxycellulose by sodium periodate
     高碘酸钠氧化纤维素的研究
短句来源
     Sodium Nitroprusside
     硝普钠
短句来源
     Sodium Nitroprusside
     注射用硝普钠
短句来源
     Insulin was labeled with horseradish peroxidase by sodium periodate oxidation method.
     用高碘酸钠氧化法将辣根过氧化物酶标记于胰岛素。
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The rabbits were immunized with calcium binding protein CaBP72 which had been shown to be homogeneous by SDS-polyacrylamide gel electro-phoresis ( SDS-PAGE). The anti-CaBP72 antibody was salted out by ammonium sulfate, and further purified by DEAE-cellulose column chromatogra-phy. The purified IgG was coupled to horseradish peroxidase by using the improved sodium periodate method. The conjugate obtained, after being purified by sephadex G-200 column chromatography, contained one molecule of enzyme and...

The rabbits were immunized with calcium binding protein CaBP72 which had been shown to be homogeneous by SDS-polyacrylamide gel electro-phoresis ( SDS-PAGE). The anti-CaBP72 antibody was salted out by ammonium sulfate, and further purified by DEAE-cellulose column chromatogra-phy. The purified IgG was coupled to horseradish peroxidase by using the improved sodium periodate method. The conjugate obtained, after being purified by sephadex G-200 column chromatography, contained one molecule of enzyme and one molecule of IgG. The linear range of ELISA for CaBP72 was 2-40 ng. The binging of CaBP72 with its antibody could be stimulated by trifluroperazine, with a maximal effective concentration of 0.8mM, where the binding ability was increased by 240%. CaBp11 showed slight ability to cross-react with anti-CaBP72 antibody; the cross-reactivity eould be made 10 times higer by mixing CaBP12 with CaBP11, although the resulting cross-reaction rate was still within 0.1%.

将SDS—PAGE纯的CaBP_(72)(钙结合蛋白,分子量72KDa)免疫家兔,获抗血清。抗血清经硫酸铵盐析粗提,再经DEAE纤维素柱层析等处理得纯化抗体。用改良的过碘酸氧化法将纯化的抗体与辣根过氧化物酶(HRP)经两步法进行偶联,经Sephadex G-200柱层析纯化得酶标结合物。其中抗体与辣根过氧化物酶的克分子之比为1:1。测定CaBP_(72)的ELISA线性检测范围为2~40ng。三氟啦嗪(TFP)可增强抗原抗体的反应性:最大作用浓度为0.8mM,此时可使抗原抗体的结合率提高240%。CaBP_(11)与抗CaBP_(72)抗体有弱交叉反应,反应率小于0.1%,但CaBP_(11)、CaBP_(12)的混合品对抗CaBP_(72)抗体的交叉反应性可提高10倍以上,但仍不超过0.1%。

Two kinds of the sulfamethyldiazine antigen were synthesised with the carrier protein of BSA or OA and their immunogenicity compared; the sheep anti rabbit γ globulin were labelled with the horseradish peroxidase (HRP) according to sodium periodate method; the ELISA on sulfamethyldiazine content in milk was developed. Affinity constant of the antibody was 1.28×10 7 (the molecular mole ratio in antigen for 1∶42) and 3.21×10 7(1∶13), respectively. The detectable concentration of sulfamethyldiazine with...

Two kinds of the sulfamethyldiazine antigen were synthesised with the carrier protein of BSA or OA and their immunogenicity compared; the sheep anti rabbit γ globulin were labelled with the horseradish peroxidase (HRP) according to sodium periodate method; the ELISA on sulfamethyldiazine content in milk was developed. Affinity constant of the antibody was 1.28×10 7 (the molecular mole ratio in antigen for 1∶42) and 3.21×10 7(1∶13), respectively. The detectable concentration of sulfamethyldiazine with ELISA revealed 5 220 μg/L (1.87×10 8 8.2×10 7 mol/L) with the detection limit being 2.4 μg/L,the recovery rate of 89% 118%,the cross reactive rate with sulfadimidine and sulfathiazole of 6.0% and 0.84%, respectively, no cross reaction with sulfadimethoxine and sulfaguaniline.

以牛血清白蛋白(BSA)为载体,合成2种不同的物质的量的比值(约1∶13和1∶42)的磺胺甲基嘧啶抗原,并比较了两者的免疫原性;以卵白蛋白(OA)为载体,合成1种包被抗原;用辣根过氧化物酶标记羊抗兔IgG,工作浓度1∶1000;建立的乳中磺胺甲基嘧啶残留ELISA测定法,抗原亲和常数分别为1.28×107(1∶42)和3.21×107(1∶13),磺胺甲基嘧啶检测质量浓度为5~220μg/L(1.87×10-8~8.2×10-7mol/L),检测限为2.4μg/L,回收率为88%~118%,与磺胺二甲基嘧啶和磺胺噻唑交叉率分别为6.0%和0.84%,与磺胺二甲氧嘧啶和磺胺脒无交叉反应

Objective: By using I-ELISA methed modified by this article authors to evaluate the clinical practical application value of the monoclonal antibody(McAb) against cysticerus cellulosae antigen in diagnosing cerebrocysticercosis. Methods: The McAb (immunoglobulin G titre 1:3200 in ascites) was separated and purified by using anhydrous ethanol at low temperature; HRP conjngated McAb was prepared by modified sodium periodate method; the antibody to cysticerus cellulosae antigen in patient's cerebrospinal...

Objective: By using I-ELISA methed modified by this article authors to evaluate the clinical practical application value of the monoclonal antibody(McAb) against cysticerus cellulosae antigen in diagnosing cerebrocysticercosis. Methods: The McAb (immunoglobulin G titre 1:3200 in ascites) was separated and purified by using anhydrous ethanol at low temperature; HRP conjngated McAb was prepared by modified sodium periodate method; the antibody to cysticerus cellulosae antigen in patient's cerebrospinal fluid and serum was determined by inhibitory ELISA(I-ELISA) method. Results: Out of 63 cerebrospinal fluid specimens from patients with clinically diaginosed cerebrocysticerosis, 61 specimens showed the anti cystscerus cellulosae antibody to be positive;positive rate 97%;53 of 60 serum specimens showed the antibody to be positive, positive rate 88%; 240 cerbrospinal fluid specimens and 240 serum specimens from patients with non cerebrocysticercous intracranial infections,10 serum specimens from patients with hepatic and pulmonary echinococcosis,25 cerebrospinal fluid specimens from patients with appendicitis and 32 serum specimens from healthy volunteers totally showed the anti cysticerus antibody to be negative. Conclusion:The MCAb against cysticerus cellulosae antigen has been developed by using routinely B cell hybridoma technique,and is of high specificity and detectable rate,and therefore, is of great clinical practise value in diagnosing cerebrocysticercosis.

目的 :评价用自制的抗猪囊虫单克隆抗体建立I-ELISA检测方法应用于临床脑囊虫病的诊断价值。方法 :用低温无水乙醇沉淀法分离纯化单抗IgG(腹水抗体效价 1∶32 0 0 ) ;用改良的过碘酸钠法制备辣根过氧化物酶标单抗 ;用抑制性酶联免疫吸附法 (I-ELISA)检测患者脑脊液和血清标本中囊虫特异性抗体。结果 :检测 6 3例确诊为脑囊虫病的患者脑脊液 ,抗体阳性 6 1例 ,阳性率为 97% ;血清 6 0例 ,抗体阳性 5 3例 ,阳性率为 88% ;其他颅内感染疾病患者脑脊液标本 2 4 0例 ,血清标本 2 4 0例 ,肝肺包虫病患者血清 10例 ,正常脑脊液 2 5例 (阑尾炎手术腰麻患者 ) ,健康志愿者血清 32例 ,检测结果均为阴性。结论 :抗囊虫抗原的单克隆抗体用常规鼠源B细胞杂交瘤技术获得 ,该单克隆抗体具有相当高的特殊性和检出率 ,对诊断脑囊虫病有很高的临床应用价值

 
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