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plate counting method
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  平板计数法
     On 7,14,21,28,35,42 and 49-old-day,10 AA broilers per group were killed and 0.5g of cecal contents were collected to isolate,incubate and enumerate bifidobacteria by the plate counting method.
     分别于7、14、21、28、35、42和49日龄随机抽取各组鸡10只剖杀后,采用平板计数法测定鸡盲肠内的双歧杆菌数量。
短句来源
     Accuracy of this method is corresponded with standard plate counting method(r≥0.99),it more sensitive,rapider,and more convenient.
     结果表明:该方法准确度与国标的平板计数法相当(r≥0.99),但更为灵敏、快速、简便。
短句来源
     Results The culture effect of SC enrichment broth was the best at 42±1 ℃ in MPN method,its lowest detection limit was 10cfu/g and the coincidence rate of the examination value and theory value (500cfu/g) was 92.0% Conclusion The MPN method is better than the plate counting method both in sensitivity and applicability.
     MPN法采用SC增菌液(42±1)℃培养效果最好,检出限10cfu/g,检测值和理论值(在500cfu/g)的符合率为92.0%。 结论MPN法无论从可操作性或是检出的灵敏度都要优于平板计数法
短句来源
     At present, determining methods of the cell concentration in fermentation liquid contain gravimetric method and plate counting method.
     目前 ,测定发酵液菌体浓度的方法有重量法、平板计数法
短句来源
     Results There are no significant differences between the microcolony technique and the plate counting method.
     结果 与常规平板计数法比较 ,差别无显著性。
短句来源
  平皿计数法
     In this paper,the effect of lignosulfonates on bacteria reproduction rate in recirculated cooling (water) has been studied by plate counting method.
     通过平皿计数法研究了不同类型木质素磺酸盐及其组分对循环冷却水中细菌繁殖速度的影响。
短句来源
  “plate counting method”译为未确定词的双语例句
     No JMC1402G could be recovered through the plate counting method while the concentration of Cu 2+ addition was 500 mg/kg soil.
     当Cu2 + 浓度达到 10 μg/mL时 ,JMC14 0 2G已不能生长。
短句来源
     No JMC1402P could be recovered through the plate counting method while the concentration of Cu2+ addition was 500 mg/Kg soil.
     对不同Ctlz十、Cd2+添加量的灭菌土壤中营腐生生活37d分离的JMC1402P进行质粒快检及砂培回接试验。
短句来源
     The determinable error in plate counting method is larger.
     平板计数法费时且误差较大。
短句来源
     Sterilization of nutritive solution for soil less cultivation by ozone was determined with dilution flat plate counting method and most probable number(MPN).
     采用稀释平板法和MPN法,测定了臭氧对无土栽培营养液的消毒杀菌作用。
短句来源
     The effect of pulsed electric field (PEF) treatment, applied in a continuous system, on Saccharomyces cerevisiae cells was investigated. Ultraviolet spectrophotometer and fluorescence polarization methods were used to investigate the cell membrane permeability and membrane fluidity respectively. The plate counting method was used to determine the viability of the cells.
     应用脉冲电场(pulsed electric field,简称PEF)连续式处理系统对酿酒酵母进行了灭菌实验,并推测了PEF对酵母细胞致死作用与细胞膜结构改变之间的关系。
短句来源
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  plate counting method
A plate counting method applicable to Dunaliella is described.
      


A membrane microcolony count method was developed to measure the amount of yeast in beverages. A specific volume of the beverage sample was filtered by suction through a cellulose mixed membrane filter (pore diameter 5 μm). The filter was placed on the agar media and incubated for a few hours, followed by staining and counting with the light microscope. The amount of yeast in 14 Cola samples were counted by using this method and the plate count method. The results correlated...

A membrane microcolony count method was developed to measure the amount of yeast in beverages. A specific volume of the beverage sample was filtered by suction through a cellulose mixed membrane filter (pore diameter 5 μm). The filter was placed on the agar media and incubated for a few hours, followed by staining and counting with the light microscope. The amount of yeast in 14 Cola samples were counted by using this method and the plate count method. The results correlated well(γ=0.96, P<0.01). The microcolony count method is rapid, taking less than 12h, simple and suitable for beverages containing above 10~3 CFU yeast per ml.

本文介绍利用混合纤维素滤膜过滤饮料样品,培养后对其上生长的微菌落进行染色和计数,定量检测饮料中酵母。14份可乐样品在滤膜上的微菌落计数与相应平板培养法计数之间相关关系为(r=0.96,P<0.01),本法迅速,不到12小时报告结果,简便,适于检测污染酵母浓度高于10~3CFU/ml的饮料。

Six different weathered lignite samples were examined by scanning electron microscopy. Few microorganisms were obsevred on lignit just excavated and only spores and short hyphae were observed on lignite samples excavated 5 months, 1 year and 4 years before sampling. When lignite samples were moistened with distilled water and incubated for 10 days, actinomycetes proliferated significantly on lignite samples that were just excavated or excavated 5 months before sampling. The growth of bacteria was observed on...

Six different weathered lignite samples were examined by scanning electron microscopy. Few microorganisms were obsevred on lignit just excavated and only spores and short hyphae were observed on lignite samples excavated 5 months, 1 year and 4 years before sampling. When lignite samples were moistened with distilled water and incubated for 10 days, actinomycetes proliferated significantly on lignite samples that were just excavated or excavated 5 months before sampling. The growth of bacteria was observed on lignite samples excavated 1 year before sampling. Fungi increased in length and in number on lignite samples excavated 4 years before sampling. These findings of microbial succession on lignite samples along with weathering were consistent with results of the plate count method; actinomycetes are the first colonizers, then bacteria and fungi are the last degrader. The dominant microorganisms were Actinosynnema sp., Streptomyces sp. and Nocardia sp. among actinomycetes, two Arthrobacter sp. among bacteria and two Aspergillus sp. among fungi.

对采自辽宁省前屯煤矿的6种不同风化程度的褐煤样品进行扫描电子显微镜观察发现:刚采掘出来的褐煤表面几乎没有微生物存在.经5个月、1年及4年堆积风化的褐煤中也只见到休眠孢子和少量菌丝.将褐煤样品在潮湿状态下培养10天后,扫描电镜观察发现:刚采出来的褐煤及经5个月风化的褐煤表面有大量放线菌生长,而且菌落周围有褐煤被降解迹象.经1年风化的褐煤中除有大量放线菌及细菌生长外,真菌也有所增加.而在经4年风化褐煤中主要是真菌明显增加.平板计数结果同样说明褐煤风化过程中微生物存在演替现象:放线菌为褐煤初期降解的主要微生物,随后是细菌,在风化程度较高的褐煤中,真菌则为优势降解菌.三株优势放线菌为诺卡氏菌(Nocardia Sp.),束丝放线菌(Actinosynnema Sp.)和链霉菌(Streptomyces sp.).两株优势细菌均为节杆菌(Arthrobacter sp.).两株曲霉为栖土曲霉(A.terricola)及褚曲霉(A.ochraceous),为褐煤风化过程的优势真菌.

The Yuehu (Moon Lake) is a lagoon-inlet system located in the Rongcheng Bay, Shandong Province, China, with an area of - 5 km~2. Development has been undergoing for aquaculture and tourism. In summer of 1999, a bacterial ecological study was carried out. By using HPC (Heterotrophic Bacterial 2216E Plate Count) and TCBS (Vibrio TCBS Plate Count ) methods, the distribution of bacteria in sediments and seawaters were also studied. We also measured conform group in seawater. The results...

The Yuehu (Moon Lake) is a lagoon-inlet system located in the Rongcheng Bay, Shandong Province, China, with an area of - 5 km~2. Development has been undergoing for aquaculture and tourism. In summer of 1999, a bacterial ecological study was carried out. By using HPC (Heterotrophic Bacterial 2216E Plate Count) and TCBS (Vibrio TCBS Plate Count ) methods, the distribution of bacteria in sediments and seawaters were also studied. We also measured conform group in seawater. The results show that the maximum of conform group MPN value is 90/L, lower than the Class I specified by the Chinese National Standard of Seawater Quality (<=10,000/L). The heterotrophic bacteria in seawater is 4.95 × 10~4-3.95 × 10~5 cfu/ml, and vibrio in seawater is 3.0 × 10~2 ---9.6 × 10~3 cuf/ml. In the sediments heterotrophic bacteria is 1 .5×10~4---3.2×10~5 cfu/ml, while vibro is in the range of 1 .0×10~3- 2.2×10~4 cfu/ml. In the Rongcheng Bay, outside the lagoon, HPC and VPC are 6.65 × 10~4 cfu/ml and 2.37× 10~3 cfu/ml, respectively Though the difference between the inside and outside the lagoon areas is not big, these values are all much higher than those in the outer seas reported in the literautre.

采用总活异养菌 2216E平板计数法(HPC, Heterotrophic Bacterial 2216EPlate Count),弧菌TCBS平板计数法(VPC,Vibrio TCBS  Plate Count),对山东荣成湾月湖水体中的细菌生物量分布进行了研究。并用多管发酵法对水体中的大肠菌群进行了检测。结果显示,大肠菌群MPN值最高为9个/1 00ml,低于我国海水水质标准(≤10000个/L)。水样中的异养菌数为4.95 × 10~(4-)3.95×10~5cfu/ml,弧菌数为3.0×10(2-)9.6x 10~3cfu/ml。泥样中的异养菌数为 1. 5 x 10(4-)3. 2 x 10~5cfu/ml,弧菌数为 10 x 10(3-)2.2x 10~4cfu/ml,与之相邻的荣成湾的菌量分别为 HPC 6.65 x 10~4cfu/ml, VPC 2. 37 x10 ~3 cfu/ml,两个海区的细菌生物量差别不大,但同现有外海水的细菌生物量资料比较,结果明显偏高,总异养菌数高出二个数量级,弧菌数高出一个数量级。

 
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