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mouse gamma interferon gene
相关语句
  小鼠γ-干扰素
     Construction and Identification of Mouse Gamma Interferon Gene Eukarotic Expressing Plasmid
     小鼠γ-干扰素真核表达质粒的构建和鉴定
短句来源
     Methods Total RNA was extracted from BALB/c mouse spleen by TRIzol reagent, the mouse gamma interferon gene was amplified by reverse transcription-polymerase chain reaction . Both γ-IFN gene and eukarotic expressing plasmid pcDNA3.1(-) were cut by endonuclease EcoRⅠand BamHⅠ,then direct cloned to construct the recombinant vector pcDNA3.1(-)γ-IFN.
     方法 从BALB/c小鼠脾脏提取总RNA ,用RT -PCR方法扩增出小鼠γ -干扰素基因 ,分别用EcoRⅠ、BamHⅠ双酶切扩增片段和pcD NA3.1(- ) ,定向克隆到真核表达质粒pcDNA3.1(- ) ,构建成真核表达质粒pcDNA3.1(- )γ -IFN .
短句来源
     Objective Clone mouse gamma interferon gene,then construct and identify the mouse gamma interferon gene eukarotic expressing plasmid.
     目的 克隆小鼠γ -干扰素 (γ -IFN)基因 ,构建并鉴定小鼠γ -干扰素真核表达质粒 .
短句来源
     Conclusions Mouse gamma interferon gene eukarotic expressing plasmid was successfully constructed ,which provides material foundation to research the expression of mouse gamma interferon gene in vivo.
     结论 成功构建了小鼠γ -干扰素真核表达质粒 ,为进一步气道内实施哮喘小鼠基因治疗打下了坚实基础 .
短句来源
  相似匹配句对
     Mouse
     鼠标
短句来源
     Study on Antitumor Activity of Mouse Gamma Delta T Lymphocyte
     γδ表型T淋巴细胞的抗肿瘤效应研究
短句来源
     THE LION AND THE MOUSE
     狮子和老鼠
短句来源
     The Effect of the Blastisation of Mouse Splenic Lymphocyte by Preparation of Gamma Germ
     芽孢杆菌制剂对小鼠脾淋巴细胞转化功能的影响
     Construction and Identification of Mouse Gamma Interferon Gene Eukarotic Expressing Plasmid
     小鼠γ-干扰素真核表达质粒的构建和鉴定
短句来源
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Objective Clone mouse gamma interferon gene,then construct and identify the mouse gamma interferon gene eukarotic expressing plasmid. Methods Total RNA was extracted from BALB/c mouse spleen by TRIzol reagent, the mouse gamma interferon gene was amplified by reverse transcription-polymerase chain reaction .Both γ-IFN gene and eukarotic expressing plasmid pcDNA3.1(-) were cut by endonuclease EcoRⅠand BamHⅠ,then direct cloned to construct the recombinant vector pcDNA3.1(-)γ-IFN....

Objective Clone mouse gamma interferon gene,then construct and identify the mouse gamma interferon gene eukarotic expressing plasmid. Methods Total RNA was extracted from BALB/c mouse spleen by TRIzol reagent, the mouse gamma interferon gene was amplified by reverse transcription-polymerase chain reaction .Both γ-IFN gene and eukarotic expressing plasmid pcDNA3.1(-) were cut by endonuclease EcoRⅠand BamHⅠ,then direct cloned to construct the recombinant vector pcDNA3.1(-)γ-IFN. The recombinants were screened by PCR ,indentified by endonuclease digestion ;then positive clone was identified by gene sequencing. Results A 500bp fragment of RT-PCR product was comfirmed by electrophoresis in agarose gels.The 500bp inserting fragment was comfirmed by both PCR method and endonuclease digestion. The nucleotide sequence of positive recombinant vector was completely correct. Conclusions Mouse gamma interferon gene eukarotic expressing plasmid was successfully constructed ,which provides material foundation to research the expression of mouse gamma interferon gene in vivo.

目的 克隆小鼠γ -干扰素 (γ -IFN)基因 ,构建并鉴定小鼠γ -干扰素真核表达质粒 .方法 从BALB/c小鼠脾脏提取总RNA ,用RT -PCR方法扩增出小鼠γ -干扰素基因 ,分别用EcoRⅠ、BamHⅠ双酶切扩增片段和pcD NA3.1(- ) ,定向克隆到真核表达质粒pcDNA3.1(- ) ,构建成真核表达质粒pcDNA3.1(- )γ -IFN .转化产物通过PCR扩增筛选 ,双酶切鉴定 ;阳性克隆进行序列分析 .结果 RT -PCR产物电泳可见一约 5 0 0bp大小目的片段 .PCR和双酶切电泳结果均证实已插入约 5 0 0bp的γ -IFN基因片段 ;阳性克隆测序结果表明克隆的小鼠γ -干扰素基因完全正确 .结论 成功构建了小鼠γ -干扰素真核表达质粒 ,为进一步气道内实施哮喘小鼠基因治疗打下了坚实基础 .

 
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