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   osteocalcin 在 生物医学工程 分类中 的翻译结果: 查询用时:0.141秒
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osteocalcin
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  骨钙素
    Influence of the surface roughness of titanium on the expression of osteocalcin in human periodontal ligament cells
    钛表面粗糙度对牙周韧带细胞骨钙素表达的影响
短句来源
    The expression of osteocalcin mRNA of bone-interface when the diamond film implant materials used
    金刚石涂膜种植材料骨界面区骨钙素mRNA表达
短句来源
    On the contrary, stretching at 1000μεand 1500μεinhabited cells proliferation, collagen type I secretion , extracellular calcium deposition ,ALP activity and osteocalcin production.
    而1000με和1500με的应变水平抑制了成骨细胞的增殖、Ⅰ型胶原合成、胞外钙基质分泌、碱性磷酸酶活力和骨钙素合成。
短句来源
    3. With Dexamethasone, β-Glycerphosphoric sodium, Vitamin C or rh-BMP2 or BMPS under optimal concentration, hMSCS may be expressed alkaline phosphatase,collagen I, collagen III and osteocalcin, and localized regions of mineralization is deteced.
    3. 适宜浓度的地塞米松、β-甘油磷酸钠、维生素C等化学药物或rh-BMP2生长因子、牛BMPS提取物,可以促进hMSCs表达碱性磷酸酶、Ι型胶原、ΙΙΙ型胶原和骨钙素
短句来源
    RT-POCR was used to examine the expressions of mRNA of the osteoblast markers: osteocalcin (OCN), alkaline phosphatase (ALP), and oesteopontin (OPN).
    应用RT PCR法检测细胞骨钙素 (OCN)、碱性磷酸酶 (ALP)、骨桥蛋白 (OPN)mRNA的表达 ,观察骨髓基质细胞的成骨细胞分化情况。
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  “osteocalcin”译为未确定词的双语例句
    They are alkaline phosphatase, type I collagen, osteocalcin, bone sialoprotein, osteopontin and osteonectin, which are collectively called bone-related genes.
    它们是:碱性磷酸酶,Ⅰ型胶原,骨钙蛋白,骨涎蛋白,骨桥蛋白和骨连蛋白。 因为它们与成骨过程密切相关,故被称为骨相关基因。
短句来源
    RT-PCR showed the β-TCP ceramics could increase osteocalcin (OCN) gene expression, this made the osteoblast into a new mature stage, improved the activity and promoted the differentiation of osteoblast.
    RT-PCR显示β-TCP陶瓷增强了成骨细胞OCN的表达,使成骨细胞进入一个新的成熟阶段,提高成骨细胞的活性,促进其向骨细胞分化。
短句来源
    PURPOSE:To evaluate the ability of ectopic osteoinduction of PDDLA/rhBMP-2/hTGF-β 1 compound in mice and investigate the effects of rhBMP-2/hTGF-β 1 on gene expression of collagen-Ⅱ,osteopontin and osteocalcin during ectopic osteogenesis and biomineralization.
    目的:评价消旋聚乳酸(PDLLA)载体材料复合重组rhBMP-2和hTGF-β1诱导小鼠体内异位成骨的能力,初步探讨其作用机制。
短句来源
    In vitro Studies about the Effects of Implanted Materials on Osteocalcin ;and Alkaline Phosphatase of Rat Osteoblasts
    硬组织替代材料对成骨细胞骨钙蛋白、碱性磷酸酶水平的影响
短句来源
    Alkaline phosphatase and collagen I usually expressed in the early stage of bone development while osteocalcin and bone sialoprotein in the late stage.
    碱性磷酸酶和I型胶原一般在成骨分化的早期高度表达,而骨钙蛋白和骨涎蛋白则在成骨分化的晚期高度表达。
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  osteocalcin
The content of osteocalcin was increased after space flights.
      
The cell morphology and viability, alkaline phosphatase and osteocalcin, as markers of osteoblast proliferation were evaluated at 3, 7 and 25 days of culture.
      
Cells cultured into the COL/β-TCP scaffold presented osteoblast phenotype, intense staining of alkaline phosphatase and increased production of osteocalcin.
      
Furthermore, the mRNA expression of type I collagen, osteocalcin and osteonectin was semi-quantitively evaluated by real-time reverse transcriptase-polymerase chain reaction (RT-PCR).
      
The osteogenic differentiation of PCs was further demonstrated by the detection of type I collagen, osteocalcin and osteonectin gene expression.
      
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To observe changes of biochemical markers of bone turnover in osteoporotic bone matrix after renal transplantation, osteocalcin, bone alkaline phosphatase(BAP), PICP (carboxyterminal propeptide of type I procollagen), ICTP (carboxyterminal cross-linked telopeptide of type I collagen), parathyroid hormone (PTH) and 25-hydroxy-vitamin D (25 OHD) were measured in 7 patients with osteoporosis followingrenal transplantation (median 6 months, range 1 to 72 months). Using restrictive criteria for defining the...

To observe changes of biochemical markers of bone turnover in osteoporotic bone matrix after renal transplantation, osteocalcin, bone alkaline phosphatase(BAP), PICP (carboxyterminal propeptide of type I procollagen), ICTP (carboxyterminal cross-linked telopeptide of type I collagen), parathyroid hormone (PTH) and 25-hydroxy-vitamin D (25 OHD) were measured in 7 patients with osteoporosis followingrenal transplantation (median 6 months, range 1 to 72 months). Using restrictive criteria for defining the presence of osteoporosis when bone mineral density (BMD) measured using dual energy X-ray absorptiometry lay more than 2 SD below the mean of normal subjects of the same age and sex. Mean serum values of bone formation markers such as osteocalcin, BAP, PICP, and bone resorption marker such as ICTP were significantly higher in patients group than those of 38 noormal subjects. Serum PTH and 25OH D levels were normal. These data suggested that this osteoporosis after renal transplantation is associated with a high bone turnover (elevated osteoblastic and osteoclastic activity) without secondary hyperparathyroidism and abnormal vitamin D metabolism.

应用放射免疫分析法和免疫放射分析法对7例已确诊有骨质疏松症的肾移植患者进行血清骨代谢指标检测。结果表明患者成骨细胞活性指标(血清特异性骨碱性磷酸酶、骨钙素和Ⅰ型前胶原蛋白羧基末端前肽)和破骨细胞功能指标(血清Ⅰ型胶原交联羧基末端肽)明显高于正常对照组。患者血清甲状旁腺素和25羟维生素D水平正常。提示肾移植后伴骨质疏松症患者的成骨细胞活性和破骨细胞活性明显增高,但无继发性甲状旁腺功能亢进和维生素D代谢异常

Isolated bone cells from calvaria of neonatal white rats were grown in DMEM and 10% fetal bovine serum culture medium in vitro and the osteoblasts were identified by cell morphologic characteristics, alkaline phophatase (AKP) activity of the cells and osteocalcin in the culture medium. Then micropipette aspiration technique and an elastic half space model were adopted to investigate the passive deformation properties and effect of colchicine on the elasticity of osteoblasts. It’s found that colchicine...

Isolated bone cells from calvaria of neonatal white rats were grown in DMEM and 10% fetal bovine serum culture medium in vitro and the osteoblasts were identified by cell morphologic characteristics, alkaline phophatase (AKP) activity of the cells and osteocalcin in the culture medium. Then micropipette aspiration technique and an elastic half space model were adopted to investigate the passive deformation properties and effect of colchicine on the elasticity of osteoblasts. It’s found that colchicine had no significant effect on Young’s modulus. This study will provide valuable experimental data for further study on the active deformation of osteoblasts in vitro or in vivo, and will be favorable to reveal mechanism of biological activities of osteoblasts.

本文以乳大鼠颅盖骨为材料,体外成功分离及培养了大鼠颅盖骨成骨细胞[1 ,2] ,并对细胞形态、细胞内碱性磷酸酶(AKP) 染色、营养液中骨钙素检测等方面进行了鉴定。在此基础上利用微管吸吮技术和线弹性半无限体模型研究了大鼠成骨细胞的被动变形特性,测定了大鼠成骨细胞的杨氏模量,并研究了秋水仙素对成骨细胞弹性性质的影响,经秋水仙素处理后的成骨细胞与正常组成骨细胞相比,其杨氏模量没有显著性改变。这一结果将为进一步研究成骨细胞的离体及在体的主动变形机制,提示成骨细胞的生命活动机理提供了有价值的实验数据

Objective: To evaluate the effects of the four implant materials, hydroxyl poly calcium sodium phosphate (HPA), hydroxyapatite (HA), bioglass ceramics (BGC) and titanium (Ti) on osteocalcin secreting amount and alkaline phosphatase activity of rat osteoblasts cultured in vitro, and expose the biological compatibility conditions of the implant materials as well as the index to estimate cytocompatibility. Methods: The powders of HPA, HA, BGC and Ti were sterilized and made into extracted solutions with 199...

Objective: To evaluate the effects of the four implant materials, hydroxyl poly calcium sodium phosphate (HPA), hydroxyapatite (HA), bioglass ceramics (BGC) and titanium (Ti) on osteocalcin secreting amount and alkaline phosphatase activity of rat osteoblasts cultured in vitro, and expose the biological compatibility conditions of the implant materials as well as the index to estimate cytocompatibility. Methods: The powders of HPA, HA, BGC and Ti were sterilized and made into extracted solutions with 199 culture medium,and the isolated osteoblasts which were taken from three day old sparague Dawley rats' calvaria, were incubated in 199 culture medium too. Then the osteoblasts were mixed and incubated into the four kinds of extracted solutions of the implant materials. After eight days of co incubation, the cells were taken out to measure alkaline phosphatase activity with velocity analysis, and the extracellular liquid was used to estimate osteocalcin secreting amount with radioisotope analysis. Results: ①Osteocalcin secreting amount of the osteoblasts co incubated with HPA, HA, BGC were as normal as the control, while that of other cells co incubated with titanium was reduced (P<0.05). ②Alkaline phosphatase activities of all the co incubated osteoblasts were as normal as the control. Conclusion: The osteocalcin secreting amount and alkaline phosphatase activity can reflect the biological compatibility of implant materials, and they may be used as indexes to evaluate biological compatibility condition of implant materials. ;

目的 :研究羟基聚磷酸钙钠 (HPA)、羟基磷灰石 (HA)、生物玻璃陶瓷 (BGC)和纯钛 (Ti) 4种硬组织替代材料对成骨细胞的骨钙蛋白 (OCN)分泌量、碱性磷酸酶 (ALP)活性的影响 ,探讨这 4种材料的生物相容性。方法 :采用SD乳鼠颅顶骨成骨细胞进行体外培养 ,把HPA、HA、BGC和Ti粉分别制成材料浸提液 ,将培养成活的成骨细胞接种于材料浸提液中 ,培养 8d后测定其OCN分泌量、ALP活性。结果 :钛对骨钙蛋白分泌量有抑制作用 (P <0 0 5 ) ,4种材料对碱性磷酸酶活性均无不良影响。结论 :OCN分泌量、ALP活性能够反映不同硬组织替代材料的生物相容性 ,有可能成为评价硬组织替代材料生物相容性的指标

 
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