METHODS: The experiments were carried out in the Beijing Institute of Neuroscience and Beijing Key Laboratory of Nerve Regeneration and Repair, Capital University of Medical Sciences between October 2003 and March 2005. ① Seventy-eight male SD rats were randomly divided into model group (n=72) and normal group (n=6).
METHODS: The experiment was carried out in the Institute of Neuroscience, the Second Military Medical University of Chinese PLA between January and June 2004. H2O2 induced damaged PC12 cells were used as the models of neuron damage induced by oxidative stress, and the cultured PC12 cells were randomized into four groups: ① normal control group: the PC12 cells were cultured in DMEM medium without serum;
METHODS: The experiment was carried out in the Institute of Neuroscience, Kunming Medical College between November 2004 and July 2005. ①The marrow cells from transgenetic green fluorescent protein mice were sieved roughly by centrifugation and adherence method with Percoll separating fluid to collect the marrow suspended cells for in vitro culture, and the growing state of cells was observed.
Department of Anatomy, Kunming Medical College. MATERIALS: The experiment was conducted in the Institute of Neuroscience of Kunming Medical College from January to March 2003. Totally 24 adult SD rats were divided randomly into 4 groups: control group, 24-hour cSCI group, 7-day cSCI group, and 21-day cSCI group with 6 rats in each group.
METHODS:The experiment was completed in the Morphological Laboratory,Institute of Neuroscience of the Fourth Military Medical University of Chinese PLA from September 2003 to January 2004. Eighteen adult female SD rats,weighing 200 to 250 g[clean degree, certification number was SCXK(Military)2002-005]were provided by the Experimental Animal Center of the Fourth Military Medical University of Chinese PLA.
MATERIALS:The experiment was conducted at the Institute of Neuroscience,Fourth Military Medical University of Chinese PLA,from April to June 2004.Primary cultured hippocampus neurons of neonatal SD rats were used in the experiment.
METHODS:The experiment was carried out in the Institute of Neuroscience,Kunming Medical College from June 2004 to December 2005.The adult SD rats were adopted in this study as models.
BACKGROUND:In vitro cultured nerve cells induced by neurotoxic amyliod βprotein(Aβ) fragments exhibit pathological changes similar to those seen in Alzheimer disease(AD).This study examines the effect of kidney tonifying traditional Chinese medicine in alleviating the pathological lesions in AD. OBJECTIVE:To assess the effect of bushen yizhi fang,a traditional Chinese medicinal formula,on the growth behavior and morphology of neurotoxic Aβ treated NG108 15 cells as an AD model. DESIGN:Completely randomized...
BACKGROUND:In vitro cultured nerve cells induced by neurotoxic amyliod βprotein(Aβ) fragments exhibit pathological changes similar to those seen in Alzheimer disease(AD).This study examines the effect of kidney tonifying traditional Chinese medicine in alleviating the pathological lesions in AD. OBJECTIVE:To assess the effect of bushen yizhi fang,a traditional Chinese medicinal formula,on the growth behavior and morphology of neurotoxic Aβ treated NG108 15 cells as an AD model. DESIGN:Completely randomized and controlled experimental study. SETTING and MATERILAS:The experiment was conducted in the Institute of Neuroscience,Second Affiliated Hospital of Guangxi College of Traditional Chinese Medicine.Sixty Wistar rats,3 months old and weighing 200 g to 250 g,were purchased from the Experimental Animal Center of First Military Medical University of Chinese PLA. INTERVENTIONS and MAIN OUTCOME MEASURES:The rats were randomized into control and treatment group,with 30 rats in each and totally gender balanced.The rats in the latter group were given gastric infusion of the extract of bushen yizhi fang for a month before their blood was collected for preparing the drug containing serum.Changes of Aβ induced NG108 15 cells,such as the viability rate, morphology and cell cycle,were assessed by cell counting,MTT assay and flow cytometry, respectively,after treatment of the cells with the prepared rat serum. RESULTS:The viability rate and number of cell processes of Aβ induced NG108 15 cells were increased after treatment with drug containing serum.Cell cycle analysis indicated an increased in S phase cell number after the treatment. CONCLUSION:Bushen yizhi fang may alleviate the neurotoxicity of Aβsegments in the nerve cells,and therefore produces therapeutic effect on AD by inhibiting the pathological progression.
BACKGROUND:Stellate ganglion block(SGB) is commonly used in pain therapy.However,the mechanisms of SGB in pain treatment are not well understood. OBJECTIVE:To investigate the effect of SGB on the concentration of serum cortisol and β endorphin of inflammatory tissue in rabbits with formalin induced pain. DESIGN:A randomized controlled experiment was conducted. SETTING and PARTICIPANTS:The experiment was performed in the Research Institute of Neuroscience,Taihe Hospital of Yunyang Medical College.Sixteen...
BACKGROUND:Stellate ganglion block(SGB) is commonly used in pain therapy.However,the mechanisms of SGB in pain treatment are not well understood. OBJECTIVE:To investigate the effect of SGB on the concentration of serum cortisol and β endorphin of inflammatory tissue in rabbits with formalin induced pain. DESIGN:A randomized controlled experiment was conducted. SETTING and PARTICIPANTS:The experiment was performed in the Research Institute of Neuroscience,Taihe Hospital of Yunyang Medical College.Sixteen healthy rabbits were randomly assigned into two groups of 8 each:SGB group and control group. INTERVENTIONS:A catheter was inserted close to the right stellate ganglion in each rabbit operatively.All of the rabbits received 0.5 mL formalin(80 g/L) stimulation by intraplantar injection into the right front paws.Ten minutes before stimulation,0.5 mL of 2.5 g/L bupivacaine was administered via the catheter in SGB group,while 0.5 mL of normal saline was applied in the control group. MAIN OUTCOME MEASURES:Serum cortisol at 10 minutes before stimulation,10,60 and 120 minutes after stimulation,and β endorphin of inflammatory tissue at 120 minutes after stimulation were measured by radioimmunological method. RESULTS:The concentration of serum cortisol increased significantly after pain stimulation at all time points compared with that before pain stimulation,and reached its peak concentration after 60 minutes in control group[(462.3±88.5) nmol/L](P< 0.01),while the concentration did not change significantly at all time points in SGB group(P >0.05).There were significant differences between SGB group and control group at 10 minutes[(146.3±54.8),(186.5±45.8) nmol/L,P< 0.05],60 minutes[(138.4±32.5),(462.3±88.5)nmol/L,P< 0.01]and 120 minutes[(140.9±55.6),(321.4±65.3) nmol/L,P< 0.01] after stimulation,but no difference at 10 minutes before stimulation[(130.2±31.7),(128.6±35.4) nmol/L,P >0.05].The β endorphin concentration of inflammatory tissue was significantly higher in SGB group[(1 675±343) pg/g]than thatin control group[(418±56) pg/g](P< 0.01) at 120 minutes after stimulation. CONCLUSION:SGB can inhibit the upregulation of serum cortisol in rabbits with formalin stimulation,and increase β endorphin concentration of inflammatory tissue.
BACKGROUND:Increasing evidences have suggested that the immune system does not operate as a closed system,but interact with the central nervous system(CNS).However,the exact components of the CNS involved in immunomodulation have not been clearly identified. OBJECTIVE:To investigate the distribution of the neurons related to neuroimmunomodulation in rat brain. DESIGN:A randomized and controlled experiment SETTING:The experiment was completed in the Department of Neurosurgery,Zhujiang Hospital,First Military...
BACKGROUND:Increasing evidences have suggested that the immune system does not operate as a closed system,but interact with the central nervous system(CNS).However,the exact components of the CNS involved in immunomodulation have not been clearly identified. OBJECTIVE:To investigate the distribution of the neurons related to neuroimmunomodulation in rat brain. DESIGN:A randomized and controlled experiment SETTING:The experiment was completed in the Department of Neurosurgery,Zhujiang Hospital,First Military Medical University of Chinese PLA,and Institute of Neuroscience,Fourth Military Medical University of Chinese PLA and the Department of Respiratory,Tangdu Hospital, Fourth Military Medical University of Chinese PLA. MATERIALS:This experiment was carried out in the Department of Neurosurgery, Zhujiang Hospital ,First Military Medical University of Chinese PLA,and Institute of Neuroscience, Fourth Military Medical University of Chinese PLA.Ten normal male adult SD rats of clean grade were purchased from the Experimental Animal Center of the Fourth Military Medical University. INTERVENTION:Rat models of immune challenge were produced by intraperitoneal lipopolysaccharide(LPS) injection for observing the distribution of Fos protein with immunohistochemical ABC method. MAIN OUTCOME MEASURES:The distribution of Fos protein in rat brain. RESULTS:Fos positivity was found mainly in the frontal parietal cortex, forebrain limbic system(cingulate cortex, piriform cortex,lateral septal nucleus and central amygdaloid nucleus),paraventricular thalamic nucleus,hypothalamic paraventricular nucleus, arcuate nucleus, supraoptic nucleus,suprachiasmatic nucleus,lateral hypothalamic area,lateral ventral part of periaqueducal gray,lateral parabrachial nucleus and medullary visceral zone.No distinct gathering of Fos proteins was found in the cerebellum. CONCLUSION:Fos positive neurons induced by LPS are extensively distributed in rat brain.
institute of neuroscience
神经科学研究所
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