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recombinant anti
相关语句
  重组抗
     Expression of recombinant anti HBx single chain phageantibody
     重组抗HBx单链噬菌体的表达
短句来源
     The successful generation of the phage displayed anti Id ScFv to MC5 might lay a foundation for screening and identification of new candidate molecules for developing recombinant anti Id vaccine of colorectal carcinoma.
     针对单抗MC5的噬菌体呈现型抗 IdScFv的制备 ,为筛选新的结肠癌重组抗 Id瘤苗候选分子奠定了基础
短句来源
     Conclusion The successful generation of antiId ScFv to monoclonal antibody MGd1 by recombinant phage antibody library technology might lay a foundation for screening of novel candidate molecules for developing recombinant antiId vaccine of gastric carcinoma.
     结论应用噬菌体抗体库技术成功制备了针对单抗MGd1的抗-IdScFv,从而为筛选新的胃癌重组抗-Id瘤苗奠定了基础。
短句来源
  “recombinant anti”译为未确定词的双语例句
     Construction and Expression of Recombinant Anti NI-35 Single Chain Antibody
     抗NI-35重组单链抗体表达载体的构建与融合表达
短句来源
     Conclusion:The phage displayed anti Id ScFv to MC3 were successfully selected by phage antibody library technology,which might provide new putative candidate molecules for developing recombinant anti Id vaccine of colorectal carcinoma.
     结论 :用噬菌体抗体库技术成功地获得了单抗MC3的anti IdScFv,从而为进行结肠癌重组anti Id疫苗作用的研究提供了候选分子
短句来源
     Objective To pave the way for developing recombinant antiidiotypic antibody(antiId)vaccine of gastric carcinoma by generating phagedisplayed antiId to monoclonal antibody MGd1 directed against the cancer.
     目的获得胃癌单抗MGd1的噬菌体呈现型抗独特型抗体(抗-Id),为研制针对MGd1的抗-Id重组胃癌瘤苗创造条件。
短句来源
  相似匹配句对
     Anti-H.
     抗H .
短句来源
     Anti-H.
     结果表明,抗H.
短句来源
     Recombinant E.
     分别以卡拉胶、明胶、海藻酸钠包埋 E.
短句来源
     Anti-thrombotic efficacy of recombinant hirudin
     重组水蛭素对血栓形成的抑制作用
短句来源
     Studies of Anti-CEA Recombinant Immunotoxin
     抗癌胚抗原重组免疫毒素的研究
短句来源
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  recombinant anti
This suggests that the technology presented here provides a simple method for using plants as an inexpensive alternative source for production of recombinant anti-rotavirus antigens.
      
In this study, recombinant anti-F5 scFv antibody fragment produced in E.
      
A recombinant anti-erbB2, scFv-Fc-IL-2 fusion protein retains antigen specificity and cytokine function
      
Using both anti-P-positive patient sera and the selected recombinant anti-P antibodies, the immunodominant epitope was determined and shown to be located at the C-terminal end of the P proteins (amino acids 111-115).
      
The selected recombinant anti-P antibodies specifically recognize the P proteins in immunofluorescence assays on HEp-2 cells and in immunoblotting assays, and they immunoprecipitate the P proteins under native conditions.
      
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Schistosoma japonicum recombinant antigens were used to immunize mice in order to ob-serve their protective immunity against challenge. It was assumed that the recombinant anti-gens could reduce worm burden (29. 2%- 51. 0%) and inibit the fecundity of female schisto-some (52. 1% - 74. 3%). The antibody titers increased significant 3 weeks after the first im-munization and remained high lever. The results indicated that the recombinant antigens ex-pressed in the E. coli could induce protective immunity...

Schistosoma japonicum recombinant antigens were used to immunize mice in order to ob-serve their protective immunity against challenge. It was assumed that the recombinant anti-gens could reduce worm burden (29. 2%- 51. 0%) and inibit the fecundity of female schisto-some (52. 1% - 74. 3%). The antibody titers increased significant 3 weeks after the first im-munization and remained high lever. The results indicated that the recombinant antigens ex-pressed in the E. coli could induce protective immunity against challenge. It might be pro-duced in large scale as an important candidate component of a multivalent vaccine against schistosomiasis japonicum.

采用日本血吸虫重组抗原加福氏佐剂免疫小鼠,攻击感染后,不仅可减少虫负荷(减虫率为29.2%—51.0%),还可降低血吸虫雌虫的产卵量(减卵率为52.1%—74.3%),抗体滴度在初次免疫后3wk即显著升高,并持续维持较高水平。结果表明,经大肠杆菌表达的日本血吸虫重组抗原能够诱导抗攻击感染的保护性免疫力,可望作为混合多价疫苗的候选组分。

Objective:To study the expression of recombinant anti HBx single chain phage antibody.Methods:The anti HBx single chain phage antibody(pScFv) was constructed for the study on active expression of genetic antibody in E.coli.The anit HBx ScFv gene was recombined with phagemid vector (pCANTAB) and transferred into host bacteria.Results:The anti HBx pScFv was induced with helper phage M13KO7.The soluble expression products fused with phage minor coat protein was secreted which was detected...

Objective:To study the expression of recombinant anti HBx single chain phage antibody.Methods:The anti HBx single chain phage antibody(pScFv) was constructed for the study on active expression of genetic antibody in E.coli.The anit HBx ScFv gene was recombined with phagemid vector (pCANTAB) and transferred into host bacteria.Results:The anti HBx pScFv was induced with helper phage M13KO7.The soluble expression products fused with phage minor coat protein was secreted which was detected specific binding affinity by Western blot and ELISA assay.Conclusion:The successful construction and expression of anti HBx pScFv might establish the method for the active selecting and preparing the specific antibodies in the E.coli expression system.

目的:进一步探索重组基因工程抗体在原核系统中有效的活性表达途径。方法:在已构建的抗HBx单链抗体(ScFv)的基础上,利用粘粒载体构建了抗HBx单链噬菌体抗体(phageantibody),并转化大肠杆菌。结果:该噬菌体抗体经辅助噬菌体M13KO7诱导和噬菌体次要衣壳蛋白(PⅢ)融合表达,并组装成噬菌体衣壳蛋白可溶性表达于培养液上清,经ELISA和Westernblot的结果证实可溶性表达产物具有HBx抗原的特异性亲和力。结论:单链噬菌体抗体的构建表达成功,可望成为原核系统中表达筛选特异性抗体可变区及有效制备活性基因工程抗体的简捷途径

Objective:To prepare the expressed DNA fragment for the construction of anti-caries DNA vaccine. Methods:To confirm that pPC41,a recombinant plasmid,contains pac gene by way of restriction enzyme digestion and electrophoresis;To choose important regions in pac gene and to design primers with computer, and then to amplify DNA sample. Restriction enzyme digestion and electrophoresis were used to identify PCR products. Results:pPC41 was verified to contain pac gene.The DNA fragment amplified from pac gene was...

Objective:To prepare the expressed DNA fragment for the construction of anti-caries DNA vaccine. Methods:To confirm that pPC41,a recombinant plasmid,contains pac gene by way of restriction enzyme digestion and electrophoresis;To choose important regions in pac gene and to design primers with computer, and then to amplify DNA sample. Restriction enzyme digestion and electrophoresis were used to identify PCR products. Results:pPC41 was verified to contain pac gene.The DNA fragment amplified from pac gene was expected one. Conclusion:The successful PCR amplification to important regions in pac gene is a significant preparation for the construction of recombinant anti-caries vaccine. [

目的 :为克隆构建DNA防龋疫苗制备出表达片段。方法 :酶切电泳鉴定含pac基因的重组质粒 ;用计算机辅助选定pac基因中重要区域并设计引物 ,对其进行PCR扩增 ,酶切电泳鉴定扩增产物。结果 :pPC4 1重组质粒包含pac基因 ;自pac基因中选定的核苷酸序列为模板所扩增的DNA片段为预期目的片段。结论 :对含pac基因重要抗原决定簇区域的成功扩增为构建基因重组防龋疫苗完成了重要的准备工作。

 
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