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   traditional gene 的翻译结果: 查询用时:0.046秒
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traditional gene
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     Function mining algorithm based on traditional Gene Expression Programming(GEP) and other improved algorithm may still lead to local optimum trap. To solve this problem,a new algorithm based on Genome Diversity-Guided(DG-GEP) in grading evolution was proposed.
     为了解决基于传统基因表达式编程(GEP)的函数挖掘及其改进算法仍然存在局部优化的缺陷这一问题,提出了以基因组多样性制导的分阶段进化挖掘算法DG-GEP。
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     When carrying the gene of hA,it made the expression of this gene in tumor cells far more effectively than the adenovirus vector employed in the traditional gene therapy.
     同时CNHK 2 0 0 hA携带人抗肿瘤新生血管生成基因hA ,能在肿瘤细胞内高效表达hA蛋白 ,其表达量高于传统基因治疗的腺病毒载体系统。
短句来源
     Mining functions from experimental data based on traditional gene expression programming (GEP) fitness mechanism falls short in handling noises, which may lead to anamorphic results The contributions of this paper include: (1)Proposing a new concept called weak adaptive model (WAM) based on GEP to break the limitation, which is enlightened by the biologic nature known as “seek advantage, avoid disadvantage”;
     用传统基因表达式编程 (GEP)适应度机制挖掘函数关系容易受到噪声干扰 ,导致结果失真 为此做了如下探索 :①借鉴生物具有的“趋利避害”天性 ,提出了GEP的“弱适应模型” ,以实现在含噪声的数据集上挖掘函数关系 ;
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     With the great development of molecular biology, gene therapy for cancer is from bench to bed. However, little advancement has been achieved in clinical research. The main reason is that the transmissions of traditional gene therapy utilize non-replicative virus or physicochemical methods.
     随着分子生物学迅速发展,肿瘤基因治疗正逐步从实验室进入临床,但是进展十分缓慢,其主要障碍是传统基因治疗采用非增殖病毒或物理化学方法等基因传输方案,基因转染率、表达量低,载体对肿瘤细胞缺乏靶向性,从而达不到有效治愈肿瘤的目的。
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     On the one hand, there is a lash between the thousands of year's traditional gene in rural society, and on the other hand, the effect of promotion profit is inadequate.
     究其原因,一方面是几千年积淀在农村社会的传统基因与不断变化的现实农村体制的冲突,另一方面是利益的拉动效应不足。
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  “traditional gene”译为未确定词的双语例句
     Conclusions: 1. HBV DNA S gene directly sequencing analysis for genotyping simplified the traditional gene sequence genotyping method ,at same
     结论:1.应用HBV DNA S基因序列直接进行基因分型,不仅简化了传统的全基因序列分型法,而且具有良好的敏感性、特异性和稳定性,且操作简便,为应用于临床和流行病学调查研究奠定了实验基础。
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     But the effect of suicide gene therapy is not satisfactory because of low efficiency of traditional gene vector such as retrovirus, liposome and the tolerance of single gene.
     但传统的载体如逆转录病毒和脂质体等的转染效率和表达效率不高,再加上单一的自杀基因疗法易产生耐药性等原因,因而自杀基因疗法的效果欠佳。
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     The traditional gene project method was used and successfully constructed eukaryotic expression plasmid pcDNA3.1-fGH and prokaryotic expression plasmids pPROEXTMHTa-fGH. The result showed that both of them had expressed in the cell.
     我们采用传统的基因工程手段克隆到fGH基因并构建了其真核表达质粒pcDNA3.1-fGH和原核表达质粒pPROEX~(TM)HTa-fGH,并且都在宿主中得到了表达。
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     Contrast to the traditional gene recombination method, this technique hasn't any influence on the construction of genome itself and doesn’t produce immunoreaction and random integration caused by virus or plasmid vectors.
     较之传统的基因重组技术,该技术对基因组本身的结构几乎没有任何影响,且不会产生因病毒载体和质粒载体引起的免疫反应以及随机整合。
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     Compared with traditional gene assay technology, it has the features of simple structure, label-free property, and fast detection speed.
     与传统的基因检测技术相比,压电基因传感器具有结构简单、不需要标记、检测时间短等特点。
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  相似匹配句对
     Gene Technology and Modernization of Traditional Chinese Medicine
     基因技术与中药现代化
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     On Traditional Cultural Gene of Kang Youwei's Opinion
     康有为思想中的传统文化基因
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     -casein gene.
     -酪蛋白基因5'侧序列。
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     The gene of xylanase B from T.
     但编码该酶的基因xynB在大肠杆菌中的表达较困难。
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     On Traditional Geography
     中国传统地理学刍议
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  traditional gene
Until now, the various proteins highly expressed in adipose tissues have been identified and characterized by traditional gene cloning techniques.
      
(1999) Am J Hum Genet 65: 1178-1193] which outperforms the traditional gene frequency method.
      
Recovery of different integron-linked genes from one of the isolates indicated the usefulness of this approach for gene mining in place of traditional gene recovery methodologies.
      
Replication-competent viruses have the potential to overcome the delivery barrier in tumors that has plagued traditional gene-therapy approaches to cancer treatment.
      
Replication-competent viruses have shown considerable promise in overcoming the inefficient gene transduction experienced by traditional gene therapy approaches to cancer treatment.
      
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Construction and screening of cDNA libraries for genes of interest represent the standard but tedious cloning procedures. The advent of PCR technology has greatly enhanced the efficiency of traditional gene isolation and cloning techniques.RACE (Rapid Amplification of cDNA Ends) is a simple, efficient, and sensitive technique for amplification of cDNAs encoding molecules with 5′ or 3′ regions of unknown sequence. Various modifications and improvements of the RACE procedure have been successfully employed.Here...

Construction and screening of cDNA libraries for genes of interest represent the standard but tedious cloning procedures. The advent of PCR technology has greatly enhanced the efficiency of traditional gene isolation and cloning techniques.RACE (Rapid Amplification of cDNA Ends) is a simple, efficient, and sensitive technique for amplification of cDNAs encoding molecules with 5′ or 3′ regions of unknown sequence. Various modifications and improvements of the RACE procedure have been successfully employed.Here we reviewed the historical perspective, modified protocal and application of RACE.

RACE:cDNA末端快速扩增技术进展明洪黄秉仁中国协和医科大学基础医学院中国医学科学院基础医学研究所国家分子生物学重点实验室北京100005基因表达水平和模式的变化驱动着生物体内主要的生物学过程。分离和克隆基因是研究基因结构、功能以及表达的基础。以...

Objective To develop a new kind of vector system, named as gene viral vector, which combines the advantages of the gene therapy and virus therapy. Method An anti tumor gene was inserted into the genome of the replicative virus specific for the tumor cells by virus recombination technology. The killing effect, report gene expression of the green fluorescence protein?expression of the anti tumor gene of mouse IL12, and the replication of the virus were observed respectively by cell pathology, fluorescence...

Objective To develop a new kind of vector system, named as gene viral vector, which combines the advantages of the gene therapy and virus therapy. Method An anti tumor gene was inserted into the genome of the replicative virus specific for the tumor cells by virus recombination technology. The killing effect, report gene expression of the green fluorescence protein?expression of the anti tumor gene of mouse IL12, and the replication of the virus were observed respectively by cell pathology, fluorescence microscopy, ELISA and electron microscopy. Results A new kind of gene viral vector system, in which the E1b 55 000 gene is deleted but the E1a gene of adenovirus is preserved, was constructed. The vector system possessed the same characteristics as the replicative virus ONYX 015, replication and proliferation in the tumor cells but not in the normal cells, thus specifically killing the tumor cells. Besides, it carried many kinds of anti tumor genes. When carrying the report gene of the green fluorescence protein it made the expression of this gene in tumor cells far more effectively than the adenovirus vector employed in the traditional gene therapy did. However in the normal cells the expression of green fluorescence protein caused by this vector system was as little as or even less than that by the traditional adenovirus system. The similar result was also observed in the experiments of this vector system carrying the anti tumor gene, gene of mouse IL12. The replication and proliferation of the virus carrying the gene of mouse IL12 in the tumor cells were confirmed by electron microscopy. Conclusion Gene viral vector is a new kind of vector in which the anti tumor gene is inserted into the genome of the replicative virus specific for the tumor cells. It increases the expression of the anti tumoe gene by hundreds even tens of thousand times. It posseses all the advantages of gene therapy and virus therapy, thus further enhancing the curative effect and it overcomes such disadvantages as low transfer rate, low expression, lack of target tropism and low anti tumor activity. It will become one of the most promising means in tumor treatment.

目的 研究一种结合肿瘤基因治疗与病毒治疗优势的新型肿瘤治疗载体系统 ,即基因 病毒载体系统。方法 利用病毒重组技术将抗癌基因插入肿瘤细胞特异性的增殖病毒的病毒基因组中 ,通过细胞病理作用、荧光显微镜、免疫酶链技术及电镜等技术分别观察病毒的杀伤效应、报告基因绿色荧光蛋白、抗癌基因小鼠白细胞介素 12表达量及病毒复制情况。结果 构建了一种新型基因 病毒载体系统 ,该载体系统腺病毒E1b 5 5 0 0 0蛋白缺失 ,保留了腺病毒E1a蛋白。该载体系统具有肿瘤增殖病毒ONYX 0 15的相似功能 ,即它可在肿瘤细胞内复制及增殖 ,而在正常细胞内不能复制及增殖 ,从而特异性杀灭肿瘤细胞。该载体系统还可携带各种抗癌基因以进一步提高抗肿瘤的疗效。应用该载体系统携带该报告基因绿色荧光蛋白可使绿色荧光蛋白在肿瘤细胞内高效表达 ,其表达量明显高于传统基因治疗的腺病毒载体系 ,而在正常细胞内低表达 ,表达量与传统腺病毒载体系统相似或更低。应用该载体系统携带抗癌基因小鼠白细胞介素 12 ,也产生类似结果。电镜也证实该载体系统携带抗癌基因小鼠白细胞介素 12可在肿瘤细胞株中复制及增殖。结论 基因 病毒载体将抗癌基因插入肿瘤增殖病...

目的 研究一种结合肿瘤基因治疗与病毒治疗优势的新型肿瘤治疗载体系统 ,即基因 病毒载体系统。方法 利用病毒重组技术将抗癌基因插入肿瘤细胞特异性的增殖病毒的病毒基因组中 ,通过细胞病理作用、荧光显微镜、免疫酶链技术及电镜等技术分别观察病毒的杀伤效应、报告基因绿色荧光蛋白、抗癌基因小鼠白细胞介素 12表达量及病毒复制情况。结果 构建了一种新型基因 病毒载体系统 ,该载体系统腺病毒E1b 5 5 0 0 0蛋白缺失 ,保留了腺病毒E1a蛋白。该载体系统具有肿瘤增殖病毒ONYX 0 15的相似功能 ,即它可在肿瘤细胞内复制及增殖 ,而在正常细胞内不能复制及增殖 ,从而特异性杀灭肿瘤细胞。该载体系统还可携带各种抗癌基因以进一步提高抗肿瘤的疗效。应用该载体系统携带该报告基因绿色荧光蛋白可使绿色荧光蛋白在肿瘤细胞内高效表达 ,其表达量明显高于传统基因治疗的腺病毒载体系 ,而在正常细胞内低表达 ,表达量与传统腺病毒载体系统相似或更低。应用该载体系统携带抗癌基因小鼠白细胞介素 12 ,也产生类似结果。电镜也证实该载体系统携带抗癌基因小鼠白细胞介素 12可在肿瘤细胞株中复制及增殖。结论 基因 病毒载体将抗癌基因插入肿瘤增殖病毒基因组 ,可数百倍乃至上万倍提高抗癌基因的表达量 ,进一步提高

Objective To develop a novel gene-viral therapeutic system which combines the advantages of the gene therapy,antiangiogenic therapy and virotherapy.Methods The antiangiogenic gene,designated human angiostatin (k1-5,hA),was inserted into the genome of the replicative virus specific for the tumor cells by virus recombination technology.The replication of the virus,the expression of the antiangiogenic gene of hA and the suppression of angiogenesis were observed respectively by virus replication assay in vitro,electron...

Objective To develop a novel gene-viral therapeutic system which combines the advantages of the gene therapy,antiangiogenic therapy and virotherapy.Methods The antiangiogenic gene,designated human angiostatin (k1-5,hA),was inserted into the genome of the replicative virus specific for the tumor cells by virus recombination technology.The replication of the virus,the expression of the antiangiogenic gene of hA and the suppression of angiogenesis were observed respectively by virus replication assay in vitro,electron microscopy,Western blot,and chicken embryo chorioallantoic membrane (CAM) assay.Results A new kind of gene-viral vector system,designated CNHK200-hA,in which the E1b55000 gene was deleted but the E1a gene of adenovirus was preserved,was constructed.The novel vector system possessed the same property as the replicative virus ONYX-015,replication and proliferation in the p53 - tumor cells and in some p53 + tumor cells but not in the normal cells,thus specifically killing the tumor cells.Besides,it carried the antiangiogenic gene,human angiostatin (k1-5),further enhancing the effect of anti-tumor.When carrying the gene of hA,it made the expression of this gene in tumor cells far more effectively than the adenovirus vector employed in the traditional gene therapy.Electron microscopy confirmed that this vector system replicated and proliferated in the lung tumor cells.CAM assay demonstrated that the hA produced by CNHK200-hA infected tumor cells possessed antiangiogenic bioactivity.Conclusion CNHK200-hA,a novel vector in which the antiangiogenic gene is inserted into the genome of the replicative virus specific for the tumor cells,can increase the expression level of antiangiogenic gene.It possesses all the advantages of gene therapy,virotherapy,and antiangiogenic therapy,thus further enhancing the curative effect and overcoming such disadvantages as low transfer rate,low expression,lack of target tropism and low anti-tumor activity.

目的 构建一种结合抗肿瘤新生血管生成的基因治疗与病毒治疗优势的新型肿瘤基因 病毒治疗系统CNHK2 0 0 hA。方法 克隆人抗血管生成基因Angiostatin(k1 5 ) ,命名为hA。利用病毒重组技术将hA插入肿瘤特异性增殖病毒的病毒基因组中 ,通过病毒增殖试验、电镜技术、Westernblot分析、鸡胚尿囊膜试验 (CAM ) ,观察CNHK2 0 0 hA的复制情况、hA基因的表达及其对新生血管生成的抑制作用。结果 构建了一种新型的基因 病毒治疗系统CNHK2 0 0 hA。该治疗系统为E1b 5 5× 10 3 蛋白缺失而保留了腺病毒E1a蛋白的 5型腺病毒 ,能在肿瘤细胞内大量增殖及复制 ,而在正常细胞内增殖能力较弱 ,从而特异性杀灭肿瘤细胞。同时CNHK 2 0 0 hA携带人抗肿瘤新生血管生成基因hA ,能在肿瘤细胞内高效表达hA蛋白 ,其表达量高于传统基因治疗的腺病毒载体系统。电镜证实该治疗系统可在肺癌A5 49细胞株中复制及增殖。CAM试验证实该治疗系统感染肺癌细胞后的培养液上清具有抗新生血管生成的生物活性。结论 基因 病毒治疗系统CNHK2 0 0 hA能在肿瘤细胞中...

目的 构建一种结合抗肿瘤新生血管生成的基因治疗与病毒治疗优势的新型肿瘤基因 病毒治疗系统CNHK2 0 0 hA。方法 克隆人抗血管生成基因Angiostatin(k1 5 ) ,命名为hA。利用病毒重组技术将hA插入肿瘤特异性增殖病毒的病毒基因组中 ,通过病毒增殖试验、电镜技术、Westernblot分析、鸡胚尿囊膜试验 (CAM ) ,观察CNHK2 0 0 hA的复制情况、hA基因的表达及其对新生血管生成的抑制作用。结果 构建了一种新型的基因 病毒治疗系统CNHK2 0 0 hA。该治疗系统为E1b 5 5× 10 3 蛋白缺失而保留了腺病毒E1a蛋白的 5型腺病毒 ,能在肿瘤细胞内大量增殖及复制 ,而在正常细胞内增殖能力较弱 ,从而特异性杀灭肿瘤细胞。同时CNHK 2 0 0 hA携带人抗肿瘤新生血管生成基因hA ,能在肿瘤细胞内高效表达hA蛋白 ,其表达量高于传统基因治疗的腺病毒载体系统。电镜证实该治疗系统可在肺癌A5 49细胞株中复制及增殖。CAM试验证实该治疗系统感染肺癌细胞后的培养液上清具有抗新生血管生成的生物活性。结论 基因 病毒治疗系统CNHK2 0 0 hA能在肿瘤细胞中增殖复制 ,并明显提高hA基因的表达量 ,表达产物具有抑制新生血管形成的作用。

 
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