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   restriction endonucleases analysis 的翻译结果: 查询用时:0.212秒
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restriction endonucleases analysis
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  酶切分析
     Through re ligation of the selected sequences from the amplified fragments, the whole CAV genomic DNA was cloned into pUC18, and the recombinant plasmid was designated as pCAV2.4. Restriction endonucleases analysis of pCAV2.4 showed that there were sites of BamHI、PstI、HindⅢ,but no site of EcoRI.
     再将其中相应序列拼接克隆进pUC18载体,获得包含CAV全基因组序列DNA片段的克隆质粒pCAV2.4。 酶切分析表明,该质粒具有预期的BamHI位点、PstI位点、HindⅢ位点,而预期的EcoRI位点消失。
短句来源
     In this study, a simple and rapid method for extracting genomic DNA from Cryptococcus neoformans was established and used as template for PCR and restriction endonucleases analysis.
     本研究建立了简单快速抽提新生隐球菌基因组DNA的方法,适用于PCR和酶切分析
短句来源
     The 5′terminus of ST 1 gene was genetically fused to 3′terminus of CPB gene in the recombinant plasmid pECB 2.The recombinant plamsid pECT ST 1 was studied in detail by restriction endonucleases analysis. The results showed that CPB ST fusion gene had positive reading frame. 
     经特定酶切分析鉴定,得到了理想重组子质粒pECBST1,CPB-ST融合基因在重组质粒pECT-ST1中的连接向位是正确的
短句来源
     We used restriction endonucleases EcoR Ⅰ and Sal Ⅰ to cleave plasmid pXST 1 containing 325bp ST 1 gene fragmant that encodes for heat stable enterotoxin of Escherichia coli,recovered the ST 1 gene fragmant,the 5′ terminus of ST 1 gene was genetically fused to 3′ terminus of CPB gene in the recombinant plasmid pECB 2.The recombinant plasmid pECT ST 1 was studies in detail by restriction endonucleases analysis. The results showed that CPB ST fusion gene had positive reading frame.
     利用已构建的另一重组质粒pXST1(带有肠毒素性大肠杆菌的耐热性肠毒素ST基因 ) ,通过EcoRⅠ和SalⅠ双酶切处理 ,将切下的ST基因片段定向连接到 pECB2重组质粒中CPB基因的下游。 经特定酶切分析鉴定 ,得到了理想重组子质粒pECB -ST1,CPB -ST融合基因在重组质粒 pECB -ST1中的连接向位是正确的
短句来源
     The PCR product of fedA was cloned into pUC18 at the EcoR Ⅰ and BamH Ⅰ sites. Restriction endonucleases analysis was used to identify the recombinant plasmid, and the recombinant plasmid pf107G was obtained.
     将该 PCR扩增产物在 Bam H 和 Eco R 位点克隆进 p UC1 8质粒载体 ,并转化大肠杆菌 TG1,再根据限制性内切酶酶切分析筛选出含有 fed A的重组质粒 pf1 0 7G.
短句来源
  “restriction endonucleases analysis”译为未确定词的双语例句
     Methods IL 18 mRNA expression was detected using reverse transcriptase ploymerase chain reaction (RT PCR), and the specificity of IL 18 cDNA was verified by restriction endonucleases analysis.
     方法 应用逆转录浆合酶链反应检测IL 18mRNA的表达 ,酶切鉴定其特异性 ;
短句来源
     ⑴Restriction endonucleases analysis of the plasmids: the plasmids pVSV-G,pGAG-POL,pMSCV,pMSCV-CED4 were obtained from the former work,and they were cut by the restriction endonucleases BspH Ⅰ, Bgl Ⅱ, AflⅡrespctively. These plasmids cut by restriction endonucleases were examined by agar electrophoresis.
     本实验承接前期工作,主要实验方法:⑴酶切鉴定质粒:限制性内切酶BspHⅠ、BglⅡ、AflⅡ分别酶切质粒pVSV-G、pGAG-POL、pMSCV、pMSCV-CED4,电泳鉴定质粒。
短句来源
     Restriction endonucleases analysis was used to identify the restriction sitesof the gD gene and polylinker of recombinant plasmid,and it showed that there were no sites of EcoRI、KpnI、HindⅢ、PstⅠ、Sma Ⅰ and PvuⅡ.
     酶切位点分析表明,该gD克隆也和已发表的MDV的RBIB株gD一样,不含有EcoRⅠ、HindⅢ、PstⅠ、SmaⅠ、pvuⅡ等酶切位点。
短句来源
     Restriction endonucleases analysis was used to identify the recombinant plasmid and the recombinant plasmid pMNE, pMG 2E were obtained and the pMNE and pMG 2E gE gene sequences were analyzed by comparing with the published sequence of MDV\|GA strain gE gene, the sequences of N and G 2 strains gE gene were shown that they were almost in accord with the sequence of GA strain gE.
     对重组 pMNE、pMG2 E质粒进行部分序列测定 ,并用DNAStar软件分析 ,结果表明 :插入序列与发表的GA株gE基因序列具有高度同源性 ,gE基因为高度保守基因。
短句来源
     The recombinants were idenified by PCR,restriction endonucleases analysis and sequencing. Results PCR assay showed that a DNA fragment of 1 568 bp could be amplified from the recombinant plasmids pGEM-T-1A1.Restriction endonucleases(KpnI and XhoI)digestion confirmed the target fragment had been inserted into pGEM-T successfully. The sequencing results verified that the target fragment had 99.9% homology with the CYP1A1 mRNA sequence in Genebank.
     结果重组质粒pGEM-T-1A1 PCR后获得了1 568 bp产物,酶切鉴定证实目的片段成功插入至pGEM-T,测序分析也进一步证明了目的片段与GeneBank中CYP1A1mRNA的序列同源性为99.9%。
短句来源
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  相似匹配句对
     THE RESTRICTION ENDONUCLEASES FROM BACILLUS
     芽孢杆菌中的限制性核酸内切酶
短句来源
     Analysis of the Chromosome Banding with Restriction Endonucleases
     用限制性内切酶诱导染色体显带的分析
短句来源
     Sister Chromatid Exchanges Induced by Restriction Endonucleases
     限制性内切酶诱发的姊妹染色单体互换
短句来源
     Analysis of Leptospiral Deoxyribonucleic Acids by Restriction Endonucleases
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短句来源
     Analysis of Herpes Simplex Virus Genomes with Restriction Endonucleases
     单纯疱疹病毒基因组的限制性核酸内切酶分析
短句来源
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  restriction endonucleases analysis
Intraspecific DNA polymorphism was evaluated through the restriction endonucleases analysis combined with pulsed-field gel electrophoresis.
      
We have isolated plasmid pIP112 (IncI1) from Salmonella panama and characterized by restriction endonucleases analysis and by recombinant DNA techniques a transposable element designated Tn1525.
      


Plasmid pBE322::Tn233(CH) DNA was partially digested with restriction endonu-cleases EcoRI or BamHI. The DNA fragments generated by partial digestion were re-ligated by T4-DNA ligase. The resulting DNA molecules were used to transform Esche-richia coli C600. A number of deleted transposition-defective mutants and deleted mutants which were still able to translocate (transposition wild-type) were isolated. The results of complementation test show that the transposition function of deleted transposition-defective...

Plasmid pBE322::Tn233(CH) DNA was partially digested with restriction endonu-cleases EcoRI or BamHI. The DNA fragments generated by partial digestion were re-ligated by T4-DNA ligase. The resulting DNA molecules were used to transform Esche-richia coli C600. A number of deleted transposition-defective mutants and deleted mutants which were still able to translocate (transposition wild-type) were isolated. The results of complementation test show that the transposition function of deleted transposition-defective mutants of Tn233(CH) can be complemented by deleted transposition wild-type in trans. Then the deletions were mapped by restriction endonucleases analysis. Thus, the location of transposition genes of Tn233(CH) was verified.

Tn233(CH)是从国内临床分离的耐药痢疾杆菌中找到的一个转座子,已经绘制了它的物理图,它与转座子Tn21基本相同。用限制性内切酶EcoRⅠ和BamHⅠ对带有转座子Tn233(CH)的质粒pBR322::Tn233(CH)进行不完全消化,如此产生的DNA片段经T4 DNA连接酶连接后转化到E.coli C600细胞中,获得了一些保留有转座功能或失去了转座功能的转座子缺失变种。互补试验的结果表明,保留有转座功能的Tn233(CH)缺失变种在反式位置上对失去了转座功能的Tn233(CH)缺失变种有互补作用。对这些缺失变种在DNA上缺失的区域进行限制图分析,确定了转座子Tn233(CH)中转座基因的位置。

The virus TvNPV, which had been isolated from Chengdu of Sichuan province in 1989, was identified as one of nuclear polyhedrosis virus. This is the result of its infection test, pathological study, ultrastructural observation of the inclusions and viripns, and the analysis of nucleic acid type. In addition, the results of its restriction endonucleases analysis and heat denaturation test show that the viral genome is a double stranded DNA molecule of 110.19 Kb in length, 75.25×106d in weight and its Tm...

The virus TvNPV, which had been isolated from Chengdu of Sichuan province in 1989, was identified as one of nuclear polyhedrosis virus. This is the result of its infection test, pathological study, ultrastructural observation of the inclusions and viripns, and the analysis of nucleic acid type. In addition, the results of its restriction endonucleases analysis and heat denaturation test show that the viral genome is a double stranded DNA molecule of 110.19 Kb in length, 75.25×106d in weight and its Tm value is 67℃, namely the G+C content is 33%. The highest content of the amino acid compositions in the inclusion protein are Asp and Glu, where as the lowest are Met and Cys.Because of its great infective ability to the larva of Trabala Vishnou Lefebure, the virus has great potential applicability in biological control.

对栗黄枯叶蛾病原物进行了组织清理分析、感染试验、核酸类型鉴别以及包涵体、病毒粒子形态等研究.同时对该病毒作了限制性内切酶分析和热变性试验,测定其核酸为双链DNA分子,分子量为75.25×10~6d;110.19kb,Tm值为67℃,(G+C)含量为33%,包涵体蛋白的氨基酸组分中Asp和Glu含量最高,Met,Cys含量较少.结果表明:该病毒为一种核型多角体病毒,对栗黄枯叶蛾幼虫具有很强的感染力.

Eighteen strains of P. maltophilia were screened for the occurrence of plasmid using four different methods. Five of them were harbor plasmids. The results of plasmid detection in different growth phase of P2 strain showed that the highest amount of plasmids in the strain was observed in stationary growth phase.The cheracteristics of plasmid of P. maltophilia P2 was investigated by methods of agarose gel electrophoresis, restriction endonucleases analysis, determination of molecular weight. The results...

Eighteen strains of P. maltophilia were screened for the occurrence of plasmid using four different methods. Five of them were harbor plasmids. The results of plasmid detection in different growth phase of P2 strain showed that the highest amount of plasmids in the strain was observed in stationary growth phase.The cheracteristics of plasmid of P. maltophilia P2 was investigated by methods of agarose gel electrophoresis, restriction endonucleases analysis, determination of molecular weight. The results indicated that P. maltophilia P2 contained only one type of plasmid, its molecular weight was 4.4X10' dolton and that the plasmid had single BamHl. Psil. Xbal EcoRl. HindⅢ sites. Thus, the plasmid of P. maltophilia P2 may be developed into a fine cloning vector.

本文用4种不同的方法对18株嗜麦芽假单胞菌是否含有质粒进行了检测,实验结果表明其中5株含有质粒。对其中的P2株不同生长期质粒存在状况的研究表明,该菌所含质粒与宿主不同生长期具有明显的相关性,其最高含量出现在稳定生长期,当细菌进入衰亡期时,其质粒量也随之减少,直至完全消失。 通过双向琼脂糖凝胶电泳、限制酶切分析以及分子量测定等方法,确定了嗜麦芽假单胞菌P2只含有1种质粒,分子量约为4.4×10~6道尔顿。有BamHⅠ、PitⅠ、XbaⅠ、EcoRⅠ和HindⅢ单一酶切位点。该质粒可望进一步改建成十分有用的克隆载体。

 
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