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human fetal tissues
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  人胚胎组织
     The Expression of Gastric Cancer Associated Antigen in Human Fetal Tissues Detected by Monoclonal Antibodies
     胃癌单克隆抗体MG系列相应抗原在人胚胎组织中的表达
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     Alternative Splicing of the FMR1 Gene in Human Fetal Tissues
     FMR1基因在人胚胎组织中的选择剪接表达
短句来源
     Distribution characteristic of NDRG2 expression in human fetal tissues
     NDRG2在人胚胎组织中的表达分布特点
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     Objective:To prepare specific antibody against the pre mRNA splicing protein Tra2α in human fetal tissues.
     目的 :制备用于检测人胚胎组织Tra2α蛋白的特异抗体。
短句来源
     Conclusion:This prepard antibody has good specificity and can be used to detect the Tra2α protein in the human fetal tissues.
     结论 :所制备的抗血清具有很好的特异性 ,可用于人胚胎组织中Tra2α蛋白的检测。
短句来源
  人胚组织
     Expression of phospholipase-γ1in human fetal tissues
     磷脂酶C-γ1在人胚组织中的表达
短句来源
     The 79 Novel Genes Expression Pattern in Human Fetal Tissues was Monitored by Using cDNA Microarray
     应用cDNA芯片分析79个新基因的人胚组织表达谱
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     LOCALIZATION OF AFP IN HUMAN FETAL TISSUES BY IMMUNOENZYMATIC,IMMUNOFLUORESCENT AND IMMUNOELECTRON MICROSCOPIC TECHNIQUES
     使用免疫酶标法、免疫荧光法和免疫酶标电镜法观察甲胎蛋白在人胚组织中的分布
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  “human fetal tissues”译为未确定词的双语例句
     After sequence analysis,RT PCR method was used to analyze the expression pattern in human fetal tissues.
     序列分析后用RT PCR方法研究胎儿组织中的表达情况。
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     THE DISTRIBUTION OF T CELL BEARING γ δ AND α β RECEPTORS IN HUMAN FETAL TISSUES
     带γδ和αβ受体的T细胞(TCR)在人胎儿组织内分布的研究
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     Results:PIGB1,a gene highly homologous to phosphatidylinositol glycan of complementation class B (PIG B),up regulated by ATRA,was cloned. PIGB1 expresses in various human fetal tissues.
     结果 :分离到一为ATRA激活表达的基因—PIGB1,该基因与人PIG B (phosphatidylinositolglycanofcomplementationclassB)基因高度同源 ,在多种胎儿组织中均有表达。
短句来源
     Contents of Zinc, Copper, Manganese and Cadmium in Human Fetal Tissues
     胎儿组织中锌、铜、锰和镉含量分析
短句来源
     In the present study, we studied developmental regulation of the expression of NHE isoforms in human fetal tissues by comparing the expression of various isoforms between two developmental stages, i e , week 11 (11 W) and week 16 (16 W).
     本文利用RT PCR观察了 4种NHE亚型的mRNA在人胎儿的两个不同发育阶段 (11周、16周 )在不同组织中的表达 ,以研究它们的发育调控。
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  human fetal tissues
At present, mammalian and human fetal tissues remain the carriers of unique information of the middle and late embryogenesis.
      
The composition of the protein-peptide complex of cryopreserved human fetal tissues and juvenile tissues of newborn rabbits was studied by spectrophotometric and chromatographic methods (liquid, thin layer, and reaction paper chromatography).
      
Effect of Transplantation of Human Fetal Tissues on Prooxidant-Antioxidant Equilibrium in the Liver and Blood Rats after Partial
      
Changes in erythroid and myeloid hemopoietic stems, T and B lymphocyte counts, and cell mitotic activity were observed in red bone marrow of BALB/c mice injected with human fetal tissues (placental extract and fetal liver suspension).
      
Cytological characteristics of red bone marrow of BALB/c mice after implantation of human fetal tissues
      
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Monoclonal antibody(McAbJH Ⅲ)was prepared by fusing mice myelo-ma cells SP2/0 with splenocytes from BALB/c mice spleen directiy immunized with BEL-7402 cells. The number of chromosomes of hybridoma cell lines(JHⅢ)ranged from 90 to100, McAbJHⅢ was of IgG2b type. Its titer of the culture supernatant and ascites was 1 :10 ̄2-10 ̄3 and over 1: 10 ̄6,respectively. ABC immunoperoxidase stain assay demonstrated thatMcAbJH Ⅲ was better correlation with hepatoma tissues. By the indirect immunofluo-rescence assay, it was...

Monoclonal antibody(McAbJH Ⅲ)was prepared by fusing mice myelo-ma cells SP2/0 with splenocytes from BALB/c mice spleen directiy immunized with BEL-7402 cells. The number of chromosomes of hybridoma cell lines(JHⅢ)ranged from 90 to100, McAbJHⅢ was of IgG2b type. Its titer of the culture supernatant and ascites was 1 :10 ̄2-10 ̄3 and over 1: 10 ̄6,respectively. ABC immunoperoxidase stain assay demonstrated thatMcAbJH Ⅲ was better correlation with hepatoma tissues. By the indirect immunofluo-rescence assay, it was observed that the McAbJHⅢ strongly reacted with BEL-7402 cells,but not with other tumor cells, tumor tissues, and normal human fetal tissues. Our reultsshowed that specificity of McAbJHⅢ was well, and its positive reaction rate was high,so itcould be taken as a kind of useful material in studies of serology and tumor target.

用肝癌细胞株BEL-7402脾内直接免疫BALB/C小鼠,取其脾细胞与小鼠骨髓瘤细胞SP2/0融合,获得一株分泌肝癌单抗的杂交瘤细胞株JHⅢ,其染色体众数为90~110.单抗JHⅢ系IgG2b亚类.杂交瘤细胞培养上清和腹水的效价分别为1:10 ̄2~10 ̄3和1:10 ̄6以上.ABC法证实它对肝癌组织有较好的相关性.IFA法也证明它对肝癌细胞BEL-7402有强的阳性反应,而对其他肿瘤细胞株和肿瘤组织以及胚胎组织都无反应,显示了单抗JHⅢ特异性较好,阳性率较高,可为血清学和肿瘤定位研究提供一种有用的材料.

The distribution of T cell bearing 78 and as receptors in human fetal tissues and organshave been examined by immunocytochemical techniques on frozen sections. The resultsshowed that the distribution of TCR cells was different from general T cells. TCR cells weredistributed in relatively fixed regions, for example, the TCR cells in thymus Were predominantly located in corn.comedullary junction and medulla. In the spleen, distribution of Y & Tand a 0 T cells was different, the Y & T cells were located in...

The distribution of T cell bearing 78 and as receptors in human fetal tissues and organshave been examined by immunocytochemical techniques on frozen sections. The resultsshowed that the distribution of TCR cells was different from general T cells. TCR cells weredistributed in relatively fixed regions, for example, the TCR cells in thymus Were predominantly located in corn.comedullary junction and medulla. In the spleen, distribution of Y & Tand a 0 T cells was different, the Y & T cells were located in the marginal zone while the a 9T cells occupied the periarteriolar lymphatic sheaths. In red pulp and sinuses, both kinds ofT cells were present in mixture. Unlike in spleen, in lymph node, only a few of TCR cellsscattered in the interfollicular area and paracortex, but not found within lymph follicles. Indigestive tract, the majority if TCR cells preferentially located in lamina propria of small intestine, while in stomach, large intestine and appdefldix, the TCR cells were rarely observed. In the liver, the TCR cells were concentrated in the preivascularly and sinusoids. Asmall numbers of TCR cells were seen in the dermis not found in the hasal layer of the epidermis of skin sections. However, the immunological reaction and physiological functions ofTCR cells existed in fetal tissues were not clear.

本文用细胞免疫化学方法,在冰冻切片上,检测了胎儿不同组织和器官内带γδ和αβ受体的T细胞(TCR)的分布,结果发现TCR细胞的分布与,般T细胞不同,有相对固定的分布区,如胸腺内TCR细胞主要分布在皮筋质交界处和髓质部;脾脏内的γδT主要位于边缘区,而αβT主要位于动脉周围淋巴鞘,在红髓和血窦两种细胞共存;淋巴结内只有少数TCR细胞位于滤泡间或副皮质,滤泡内则未见。消化管内的TCR细胞主要分布在小肠的固有膜,而胃、大肠和阑尾的固有膜内很少见;肝内TCR细胞主要集中在血管和血窦周围;皮肤切片内的少数TCR细胞见于真皮内,表皮基底层细胞内未见。这些细胞在胎儿期的免疫皮应及其生理功能还不清楚。

Our previous work showed the existence of low molecular weight tumor suppressors in human fetal tissues. In this paper, two tumor cell suppressors were isolated andpurified from methanol extract of human fetal liver by C18 reversed-phase mediumpressure chromatography, gel filtration on Sephadex LH-20, and high-performance liquid chromatography, directed by suppression of growth of HL-60 cells. The structuresof the suppressors were identified to be 7-ketocholesterol and 7-β-hydroxycholesterol.Under...

Our previous work showed the existence of low molecular weight tumor suppressors in human fetal tissues. In this paper, two tumor cell suppressors were isolated andpurified from methanol extract of human fetal liver by C18 reversed-phase mediumpressure chromatography, gel filtration on Sephadex LH-20, and high-performance liquid chromatography, directed by suppression of growth of HL-60 cells. The structuresof the suppressors were identified to be 7-ketocholesterol and 7-β-hydroxycholesterol.Under the condition of in vitro agar plate culture, 7--ketocholesterol and 7-β-hydroxycholesterol showed preferentially inhibitory effects on the growth of both human andmurine leukemic cell lines including human HL-60 and murine S-180 cells, but less effective on the growth of normal human and murine bone marrow granulocytemacrophage progenitors (CFU-GM).

胎儿组织中存在低分子肿瘤抑制物。胎肝细胞的甲醇-丙酮提取物中保留有大部分抑瘤活性。用体外液体培养条件下对人急性粒系白血病细胞系(HL-60)的抑制作用为指标,跟踪分离过程。提取物经反相C18中压液相色谱、SephadexLH-20凝胶色谱及正相高效液相色谱分离得到两种活性物质,经NMR和HRMS鉴定为7-酮基胆固醇(7-ketocholesterol,7-KC)和7-β-羟基胆固醇(7-β-hydroxycholesterol,7-β-HC)。体外琼脂培养条件下7-KC对小鼠S-180细胞较对正常小鼠骨髓粒-巨噬系祖细胞有更强的抑制增殖及集落生成作用。7-KC在浓度15μg/ml之内对正常人骨髓CFU-GM基本无抑制作用,但对HL-60细胞却有明显的抑制增殖作用,7-β-HC对HL-60细胞增殖较对正常人骨髓CFU-GM有更强的抑制作用。

 
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