助手标题  
全文文献 工具书 数字 学术定义 翻译助手 学术趋势 更多
查询帮助
意见反馈
   race technique 的翻译结果: 查询用时:0.009秒
图标索引 在分类学科中查询
所有学科
生物学
农作物
更多类别查询

图标索引 历史查询
 

race technique
相关语句
  race技术
     In order to study the molecular structure of reindeer β-defensin-1(reBD-1) gene,the authentic 3′end sequence of reindeer β-defensin-1(reBD-1) gene was successfully cloned by the 3′RACE technique with a specific upper primer designed according to the known reBD-1 partial cDNA fragment and the 3 sites Adaptor Primer as reverse primer.
     为进一步研究驯鹿β-防御素-1(reBD-1)基因的分子结构,利用已克隆出的reBD-1部分片段,设计了1条特异性上游引物,并以反转录引物中的部分序列即3 sites Adaptor Primer作为下游引物,采用3′RACE技术成功克隆了reBD-1 cDNA的3′末端序列。
短句来源
     The Application of RACE Technique to Clone the Full-Length cDNA of A Novel Leukemia Associated Gene LRP16
     RACE技术在钓取白血病相关基因LRP16全长cDNA中的应用
短句来源
     4. RACE technique was successfully used to amplify of a cDNA from leaves of wheat-rye 1BL/1RS alien translocation line 22/2439 induced with Bgt. A full-length cDNA clone obtained is high homologous to barley EDR1 gene, It was named as TaEDR1 (GenBank accession AY743662).
     4.用RACE技术对经白粉菌诱导24 h的小麦-黑麦1BL/1RS易位系99/2439叶片的cDNA进行扩增,获得了与大麦HvEDR1基因高度同源的小麦TaEDR1基因全长cDNA克隆(GenBank登录号:AY743662)。
短句来源
     Using homology gene clone method, with the technique of RT-PCR, two cDNA clones from Brassica pekinensis,KT680 and RT750, were obtained. The 5'-UTR and 3' -UTR of RT680 and the 3'-UTR of RT750 were further acquired using RACE technique.
     本研究采用同源基因克隆法,首先利用RT-PCR技术从耐低温植物大白菜中得到了两个cDNA克隆,即RT680和RT750,再通过RACE技术分别获得了RT680的3′端、5′端及RT750的3′端。
短句来源
     2. RACE technique was successfully used to amplify of a cDNA from leaves of wheat-rye 1BL/1RS alien translocation line 22/2439 induced with Bgt. A full-length cDNA (GenBank accessionAY584533) clone was obtained, it belongs to a receptor-like kinase gene family in wheat, and named as TaLRK.
     2.用RACE技术对经白粉菌诱导24 h的小麦-黑麦1BL/1RS易位系99/2439叶片的cDNA进行扩增,获得了小麦类受体蛋白激酶基因(RLK)全长cDNA克隆(TaLRK)(GenBank登录号:AY584533)。
短句来源
更多       
  “race technique”译为未确定词的双语例句
     Cloning STGA3 cDNA 5′ End by SMART RACE Technique
     利用SMART RACE克隆STGA3 cDNA的5′末端
短句来源
     Rapid amplification of cDNA ends (RACE) was used to amplify full length HA and NA genes of A/Chicken/Shanghai/F/98(H9N2). In the RACE technique, one anchor primer was redesigned according to the structure characteristics of 5 ′ and 3 ′ regions in all avian influenza virus genes.
     根据禽流感病毒 ( AIV)各基因片段两端保守区序列设计锚引物 ,应用 c DNA末端快速扩增法获得了 AIV分离株 A/Chicken/Shanghai/F/98( H9N2 )的血凝素 ( HA)和神经氨酸酶 ( NA)全长基因。
短句来源
     Using SMART RACE technique,we obtained the full length of novel gene of GYLZ RCC18.We also identified that GYLZ RCC18 family contains 3 subtype genes.
     应用SMARTRACE技术获得GYLZ RCC18基因的全长 ,并证明GYLZ RCC18是一个 5′端有D3种不同剪切方式亚型的基因家族。
短句来源
     Degenerate primers of actins' coding region were designed and 5'RACE technique was employed to obtain the actin cDNA of Setaria italica.
     根据植物肌动蛋白基因编码区的两端的保守序列设计了简并引物 ,用 5’RACE方法扩增出了谷子肌动蛋白基因编码区序列。
短句来源
     Results DNA sequencing confirmed that the sequence of ECRG 1 was identical to that of the previously obtained by 5′ RACE technique.
     结果 RT PCR所分离的ECRG 1基因蛋白编码序列 ,经测序证明与 5′ RACE方法钓取的该基因序列相符。
短句来源
更多       
  相似匹配句对
     technique.
     这一技术可以增加3dB带宽。
短句来源
     Diagnosis and Analysis of Nikolajewa’s Race Walking Technique
     尼科拉耶娃竞走技术诊断及分析
短句来源
     Vibratory Stress Relief Technique and Its Apply in the Race Orbit
     振动时效技术及在座圈轨道的应用
短句来源
     P2P Technique
     P2P技术
短句来源
     Research on Race and Hazard
     竞争冒险现象研究
短句来源
查询“race technique”译词为用户自定义的双语例句

    我想查看译文中含有:的双语例句
例句
为了更好的帮助您理解掌握查询词或其译词在地道英语中的实际用法,我们为您准备了出自英文原文的大量英语例句,供您参考。
  race technique
In order to determine the 5' end of the transcript, the rapid amplification of cDNA end (5'-RACE) technique was applied.
      
The full-length EPSPS cDNA sequence (CaEPSPS, GenBank accession number: AY639815) was cloned and characterized for the first time from woody plant, Camptotheca acuminata, using rapid amplification of cDNA ends (RACE) technique.
      
A new full-length β-1,3-glucanase cDNA, MpGlu, was isolated from a plantain (Musa paradisica) by the rapid amplification of cDNA ends (RACE) technique.
      
Transcripts containing long terminal repeats (LTRs) of this group were amplified from callus cDNA by the 3'RACE technique.
      
We provide evidence to show that one of the two CMS1-specific copies is actively transcribed, and two transcripts which terminate at the same position but differ in their 5'initiation sites were localized using the RACE technique.
      
更多          


Basing on the amino acid sequences of 7 kD antifungal protein from Phytolacca americana ( Hu Zhong et al,1991 ), We designed a pair of degenerated primers. By using 3'-RACE technique, we have amplified a cDNA fragment about 350 bp from the total RNA of the seeds of Phytolacca americana. PCR product was cloned into pGEM-T vectoy system directly, and the cDNA sequences was then determinated. The results indicated that the cDNA fragment contains an encoding region about 114 bp, and that the deduced...

Basing on the amino acid sequences of 7 kD antifungal protein from Phytolacca americana ( Hu Zhong et al,1991 ), We designed a pair of degenerated primers. By using 3'-RACE technique, we have amplified a cDNA fragment about 350 bp from the total RNA of the seeds of Phytolacca americana. PCR product was cloned into pGEM-T vectoy system directly, and the cDNA sequences was then determinated. The results indicated that the cDNA fragment contains an encoding region about 114 bp, and that the deduced amino acid sequences is the same as the 36 amino acids of the PAFP protein,except the two amino acids of the No.35 and No.36, at which Gln and Ile is replaced by Cys and Lys respectively. In addition, the encoding region was cloned. The cDNA may encodea a new antifungal protein of 38 amino acids.

根据美洲商陆(Phytolacaamericana)种子中一种抗真菌蛋白PAFP(Huetal,1991)的N端部分氨基酸顺序,设计、合成一条5’端寡核苷酸引物,通过3’-RACE技术从种子总RNA扩增出一约350bp的cDNA片段,成功地克隆到pGEM-T载体系统中。序列分析表明该cDNA含有114bp的编码区,由此推导的38个氨基酸序列有36个与PAFP的序列相同,仅在第35、36位氨基酸分别由Cys、Lys替代了后者的Gln和Ile。114bp的编码序列也被克隆。该cDNA可能编码一种新的38个氨基酸的抗真菌蛋白。

Objective:The purpose of this paper is to clone novel leukemia associated gene in acute myeloid leukemia (AML).Methods:The Restriction landmark genomic scanning (RLGS),Southern blot, Northern blot and rapid amplification of cDNA end (RACE) technique were performed on genomic DNA or RNA from AML and normal bone marrow (BM) samples.Results:An abnormal 2948bp fragment due to a distinct DNA methylation on NotI site in relapse sample from AML was cloned. The Sequence represents part of a new gene LRP16...

Objective:The purpose of this paper is to clone novel leukemia associated gene in acute myeloid leukemia (AML).Methods:The Restriction landmark genomic scanning (RLGS),Southern blot, Northern blot and rapid amplification of cDNA end (RACE) technique were performed on genomic DNA or RNA from AML and normal bone marrow (BM) samples.Results:An abnormal 2948bp fragment due to a distinct DNA methylation on NotI site in relapse sample from AML was cloned. The Sequence represents part of a new gene LRP16 which is located on chromosome 11q11. The full length cDNA sequence 980bp was cloned by RACE technique. Conclusion:The results indicate that RLGS and RACE technique are effective methods to find leukemia associated gene in AML. The finding of LRP16 gene is important to investigate mechanism of AML.

发现新的白血病相关基因及探讨白血病的发生机制。方法 :应用分子克隆技术RLGS及RACE技术自急性髓细胞白血病骨髓标本中克隆新的白血病相关基因。结果 :克隆到一个长度为 2 948bp的DNA片段 ,经国际基因库同源检索证明为一未知基因的一部分 ,并定位于第 11号染色体长臂 11区 ,进一步克隆到这一基因的全长cDNA ,其长度为 980bp ,并被国际基因库以新的人类基因LRP16收录入库。结论 :RLGS及RACE技术对于发现白血病相关基因是一种有效的方法 ,LRP16基因的发现对于探讨白血病发生机制具有一定意义。

Objective[WT5”BZ] To clone and identify RCC specially expressed genes different with normal kidney tissue. [WT5”HZ]Methods[WT5”BZ] A technique called suppression subtractive hybridization was used to construct the library which contains the differently expressing cDNAs between RCC and normal kidney. Then the RCC specially expressed genes were cloned from it. [WT5”HZ]Results[WT5”BZ] Human RCC subtractive library with high subtractive efficiency was set up successfully. The amplified library contains...

Objective[WT5”BZ] To clone and identify RCC specially expressed genes different with normal kidney tissue. [WT5”HZ]Methods[WT5”BZ] A technique called suppression subtractive hybridization was used to construct the library which contains the differently expressing cDNAs between RCC and normal kidney. Then the RCC specially expressed genes were cloned from it. [WT5”HZ]Results[WT5”BZ] Human RCC subtractive library with high subtractive efficiency was set up successfully. The amplified library contains 350 positive clones.Sequence analysis was performed in 5 clones. All of the sequences were unknown before and the cDNA inserting GYLZ RCC18 had three copies. Northern blot analysis showed that GYLZ RCC18 cDNA expressed highly in RCC, but there was no any signal could be detected in normal kidney.Using SMART RACE technique,we obtained the full length of novel gene of GYLZ RCC18.We also identified that GYLZ RCC18 family contains 3 subtype genes. [WT5”HZ]Conclusions[WT5”BZ] The highly efficient cDNA subtractive library lays a solid foundation for screening and cloning new and specific oncogenes or tumor suppressor genes of RCC.The novel differentially expressed genes provide an important clue to studying the mechanism of the occurrence and develpment of RCC. [WT5”HZ]

目的 克隆并鉴定肾癌与正常肾组织之间差异表达的基因 ,为研究肾癌发生发展机制提供新的突破口。 方法 应用抑制性消减杂交技术 ,构建人肾癌组织与正常肾组织差异表达的cDNA消减文库 ,并从中克隆鉴定出肾癌特异表达的基因。结果 :构建成功高消减效率的人肾癌组织cDNA消减文库 ,对其中 10个克隆的插入cDNA片段进行测序后经基因库检索表明 10个片段均为未知新序列 ,其中GYLZ RCC18基因为 5个拷贝 ,这提示以上 10个cDNA片段可能来自 6个新基因。差异分析显示GYLZ RCC18在肾癌组织中有明显表达 ,而在正常肾组织中无表达。应用SMARTRACE技术获得GYLZ RCC18基因的全长 ,并证明GYLZ RCC18是一个 5′端有D3种不同剪切方式亚型的基因家族。 结论 GYLZ RCC18基因是肾癌特异表达的新基因。人肾癌cDNA消减文库可作为筛选、克隆肾癌特异性表达的未知新基因的基础。

 
<< 更多相关文摘    
图标索引 相关查询

 


 
CNKI小工具
在英文学术搜索中查有关race technique的内容
在知识搜索中查有关race technique的内容
在数字搜索中查有关race technique的内容
在概念知识元中查有关race technique的内容
在学术趋势中查有关race technique的内容
 
 

CNKI主页设CNKI翻译助手为主页 | 收藏CNKI翻译助手 | 广告服务 | 英文学术搜索
版权图标  2008 CNKI-中国知网
京ICP证040431号 互联网出版许可证 新出网证(京)字008号
北京市公安局海淀分局 备案号:110 1081725
版权图标 2008中国知网(cnki) 中国学术期刊(光盘版)电子杂志社