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race techniques
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  race技术
     (2) Three OBP genes of H.armigera (the accession numbers ofPBP-Harm, GOBPl-Harm and GOBP2-Harm are AJ278992, AY049739 and AJ278991 in GenBank, respectively)and a GOBP2 gene of Spodoptera exigua (the accession number of GOBP2-Sexi is AJ294809 in GenBank) were cloned by PCR and RACE techniques.
     (2)利用PCR结合RACE技术克隆了3个棉铃虫OBP基因(PBP-Harm、GOBP1-Harm、GOBP2-Harm,GenBank中序列号分别为AJ278992、AY049739和AJ278991)和一个甜菜夜蛾GOBP2基因(GOBP2-Sexi,GenBank中序列号为AJ294809)。
短句来源
     Development and Application of Differential Display and RACE Techniques
     差异显示和RACE技术的发展与应用
短句来源
     According to the known SSH fragments designed primer, a novel S-Adenosy-L-Methionine synthase gene (Mu-sams2) has been cloned from banana by RACE techniques.
     根据已知SSH片段设计引物,采用RACE技术从香蕉中获得了一个新的S-腺苷-L-蛋氨酸合成酶基因,长度为1585bp,将其命名为Mu-sams2。
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     A new full_length cDNA was obtained by 5′_RACE and 3′_RACE techniques.
     基因同源性为 82 %。 结合 5′RACE和 3′RACE技术 ,获得了全长cDNA ,其为 6 72bp。
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     In this paper,using RT-PCR and RACE techniques,a gene that encodes a product belonging to the Lon protease family was isolated from wheat.This gene,designated as TaLon1 with similarity of 94% to Lon1 in indica rice and 92% to Lon1 in maize,was predicted to encode an 886 amino acid protein.
     本研究应用RT-PCR和RACE技术,克隆了小麦Lon蛋白酶家族的一个基因,命名为TaLon1。 TaLon1和籼稻Lon1、玉米Lon1的相似性分别为94%和92%。
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  “race techniques”译为未确定词的双语例句
     Based on the EST sequence of glutathione S-transferase gene from Tamarix androssowii (TaGST) published on GenBank, a TaGST cDNA was cloned with 3' and 5' RACE techniques. The full length of TaGST was 1 175 bp with ORF of 672 bp that encoded 224 amino acid residues(GenBank accission No. DQ683363).
     根据GenBank已公布的植物谷胱甘肽硫转移酶基因EST序列设计引物,利用3'和5'RACE方法,获得紫杆柽柳GST基因(TaGST)全长cDNA序列,全长为1175bp,开放读码区672bp,编码224个氨基酸(GenBank accission No.DQ683363)。
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     The cDNA of GbKTNl gene was isolated from 10 DPA fiber cells of G. barbadense using 5'RACE/3'RACE techniques.
     用5’RACE/3’RACE从海岛棉7124开花后10d的纤维细胞中分离获得了GbKTN1的cDNA序列。
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     A new full-length cDNA clone encoding phytoene desaturase gene was isolated from stigma of saffron using RT-PCR and RACE techniques( (GenBank accession No. AY 183118) ).
     本文对番红花资源的研究概况进行了概述,并依据GenBank中八氢番茄红素脱氢酶基因的保守序列设计简并引物,通过反转录聚合酶链式反应和快速分离cDNA末端技术从番红花柱头中扩增得到了八氢番茄红素脱氢酶基因的全长cDNA(GenBank AY183118)。
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     In an effort to understand the regulation of ethylene responses in flower senescence, we isolated and characterized three genes, LERS1、PLETR1 and LCTR1, which respectively exhibited high similarity to ERS1 、 ETR1 and CTR1 in Arabidopsis, using a combination of reverse transcription PCR and RACE techniques from lily petals (Z. ×L formolongi Hort. )
     本文以新铁炮百合(L.×L.formolongi Hort.)为材料,采用RT-PCR、RACE等分子生物学技术,从百合花瓣中分离得到编码乙烯受体LERS1基因cDNA全长序列和PLETR1基因cDNA保守区序列,得到了LCTR1基因3’端cDNA序列。
短句来源
     Methods The primers were designed according to the CYP4E2r6 gene fragment we reported (GenBank Accession Number: CB074945). CYP4E2r6 gene was isolated from deltamethrin-resistant the 4 th instar larvae of the mosquito using RT-PCR and RACE techniques.
     方法根据已扩增的CYP4E2r6基因片段(长470bp,GenBank登录号为CB074945)的序列设计2条特异引物,从淡色库蚊抗溴氰菊酯品系Ⅳ龄幼虫提取RNA,反转录成cDNA,以cDNA为模板,用5′-RACE和3′-RACE方法,各获得722bp、617bp片段。
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  相似匹配句对
     Development and Application of Differential Display and RACE Techniques
     差异显示和RACE技术的发展与应用
短句来源
     Biomechnic Analysis of Techniques forFootwork in Hurdle Race
     跨栏步技术的生物力学分析
短句来源
     NET techniques.
     NET的需求按重要性进行了分级,并在需求重要性的基础上给出了整个CS.
短句来源
     D3S Techniques
     D3S技术
短句来源
     On Another Definition of Race
     别种定义浅析
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  race techniques
In the present study, we determined the complete nucleotide sequence of the M-1 strain using RT-PCR and RACE techniques.
      
grandis larvae were isolated using RT-PCR followed by 5' and 3' RACE techniques.
      
A cold-regulated gene (cor tmc-ap3) coding for a putative chloroplastic amino acid selective channel protein was isolated from cold-treated barley leaves combining the differential display and the 5'-RACE techniques.
      
A cDNA corresponding to the nitrate reductase (NR) gene from Dunaliella salina was isolated by RT-PCR and (5'/3')-RACE techniques.
      
The cDNA of a marine fish microsomal epoxide hydrolase (mEH) gene from Mugil cephalus was cloned by rapid amplification of cDNA ends (RACE) techniques.
      
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Basing on the amino acid sequences of 7 kD antifungal protein from Phytolacca americana ( Hu Zhong et al,1991 ), We designed a pair of degenerated primers. By using 3'-RACE technique, we have amplified a cDNA fragment about 350 bp from the total RNA of the seeds of Phytolacca americana. PCR product was cloned into pGEM-T vectoy system directly, and the cDNA sequences was then determinated. The results indicated that the cDNA fragment contains an encoding region about 114 bp, and that the deduced...

Basing on the amino acid sequences of 7 kD antifungal protein from Phytolacca americana ( Hu Zhong et al,1991 ), We designed a pair of degenerated primers. By using 3'-RACE technique, we have amplified a cDNA fragment about 350 bp from the total RNA of the seeds of Phytolacca americana. PCR product was cloned into pGEM-T vectoy system directly, and the cDNA sequences was then determinated. The results indicated that the cDNA fragment contains an encoding region about 114 bp, and that the deduced amino acid sequences is the same as the 36 amino acids of the PAFP protein,except the two amino acids of the No.35 and No.36, at which Gln and Ile is replaced by Cys and Lys respectively. In addition, the encoding region was cloned. The cDNA may encodea a new antifungal protein of 38 amino acids.

根据美洲商陆(Phytolacaamericana)种子中一种抗真菌蛋白PAFP(Huetal,1991)的N端部分氨基酸顺序,设计、合成一条5’端寡核苷酸引物,通过3’-RACE技术从种子总RNA扩增出一约350bp的cDNA片段,成功地克隆到pGEM-T载体系统中。序列分析表明该cDNA含有114bp的编码区,由此推导的38个氨基酸序列有36个与PAFP的序列相同,仅在第35、36位氨基酸分别由Cys、Lys替代了后者的Gln和Ile。114bp的编码序列也被克隆。该cDNA可能编码一种新的38个氨基酸的抗真菌蛋白。

Objective:The purpose of this paper is to clone novel leukemia associated gene in acute myeloid leukemia (AML).Methods:The Restriction landmark genomic scanning (RLGS),Southern blot, Northern blot and rapid amplification of cDNA end (RACE) technique were performed on genomic DNA or RNA from AML and normal bone marrow (BM) samples.Results:An abnormal 2948bp fragment due to a distinct DNA methylation on NotI site in relapse sample from AML was cloned. The Sequence represents part of a new gene LRP16...

Objective:The purpose of this paper is to clone novel leukemia associated gene in acute myeloid leukemia (AML).Methods:The Restriction landmark genomic scanning (RLGS),Southern blot, Northern blot and rapid amplification of cDNA end (RACE) technique were performed on genomic DNA or RNA from AML and normal bone marrow (BM) samples.Results:An abnormal 2948bp fragment due to a distinct DNA methylation on NotI site in relapse sample from AML was cloned. The Sequence represents part of a new gene LRP16 which is located on chromosome 11q11. The full length cDNA sequence 980bp was cloned by RACE technique. Conclusion:The results indicate that RLGS and RACE technique are effective methods to find leukemia associated gene in AML. The finding of LRP16 gene is important to investigate mechanism of AML.

发现新的白血病相关基因及探讨白血病的发生机制。方法 :应用分子克隆技术RLGS及RACE技术自急性髓细胞白血病骨髓标本中克隆新的白血病相关基因。结果 :克隆到一个长度为 2 948bp的DNA片段 ,经国际基因库同源检索证明为一未知基因的一部分 ,并定位于第 11号染色体长臂 11区 ,进一步克隆到这一基因的全长cDNA ,其长度为 980bp ,并被国际基因库以新的人类基因LRP16收录入库。结论 :RLGS及RACE技术对于发现白血病相关基因是一种有效的方法 ,LRP16基因的发现对于探讨白血病发生机制具有一定意义。

Objective[WT5”BZ] To clone and identify RCC specially expressed genes different with normal kidney tissue. [WT5”HZ]Methods[WT5”BZ] A technique called suppression subtractive hybridization was used to construct the library which contains the differently expressing cDNAs between RCC and normal kidney. Then the RCC specially expressed genes were cloned from it. [WT5”HZ]Results[WT5”BZ] Human RCC subtractive library with high subtractive efficiency was set up successfully. The amplified library contains...

Objective[WT5”BZ] To clone and identify RCC specially expressed genes different with normal kidney tissue. [WT5”HZ]Methods[WT5”BZ] A technique called suppression subtractive hybridization was used to construct the library which contains the differently expressing cDNAs between RCC and normal kidney. Then the RCC specially expressed genes were cloned from it. [WT5”HZ]Results[WT5”BZ] Human RCC subtractive library with high subtractive efficiency was set up successfully. The amplified library contains 350 positive clones.Sequence analysis was performed in 5 clones. All of the sequences were unknown before and the cDNA inserting GYLZ RCC18 had three copies. Northern blot analysis showed that GYLZ RCC18 cDNA expressed highly in RCC, but there was no any signal could be detected in normal kidney.Using SMART RACE technique,we obtained the full length of novel gene of GYLZ RCC18.We also identified that GYLZ RCC18 family contains 3 subtype genes. [WT5”HZ]Conclusions[WT5”BZ] The highly efficient cDNA subtractive library lays a solid foundation for screening and cloning new and specific oncogenes or tumor suppressor genes of RCC.The novel differentially expressed genes provide an important clue to studying the mechanism of the occurrence and develpment of RCC. [WT5”HZ]

目的 克隆并鉴定肾癌与正常肾组织之间差异表达的基因 ,为研究肾癌发生发展机制提供新的突破口。 方法 应用抑制性消减杂交技术 ,构建人肾癌组织与正常肾组织差异表达的cDNA消减文库 ,并从中克隆鉴定出肾癌特异表达的基因。结果 :构建成功高消减效率的人肾癌组织cDNA消减文库 ,对其中 10个克隆的插入cDNA片段进行测序后经基因库检索表明 10个片段均为未知新序列 ,其中GYLZ RCC18基因为 5个拷贝 ,这提示以上 10个cDNA片段可能来自 6个新基因。差异分析显示GYLZ RCC18在肾癌组织中有明显表达 ,而在正常肾组织中无表达。应用SMARTRACE技术获得GYLZ RCC18基因的全长 ,并证明GYLZ RCC18是一个 5′端有D3种不同剪切方式亚型的基因家族。 结论 GYLZ RCC18基因是肾癌特异表达的新基因。人肾癌cDNA消减文库可作为筛选、克隆肾癌特异性表达的未知新基因的基础。

 
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