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   race methods 的翻译结果: 查询用时:0.165秒
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race methods
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  race技术
     A full-length cDNA of cab gene was cloned from the first strand of bamboo(Bambusa oldhamii) cDNA through RT-PCR and RACE methods, named as cab-BO1 (cab gene from B. oldhamii).
     采用RT-PCR技术与RACE技术,从绿竹中克隆到捕光叶绿素a/b结合蛋白基因全长cDNA,命名为cab-BO1(GenBank登记号EF061137)。
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     In order to study its lipid metabolism as well as its evolutional relationship with other lower percoids, we used RT-PCR and RACE methods to get the cDNA sequences of NPY, UCP2, LPL and HL gene, and deduced their corresponding amino acid sequences.
     为了研究大口黑鲈的脂代谢以及大口黑鲈在进化中的地位,我们采用RT-PCR和RACE技术分离、测定了大口黑鲈NPY基因cDNA全序列,UCP2、LPL、HL基因cDNA核心片段,并推测得到其相应氨基酸序列。
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     A novel transcript of human HPO (HPO-205) encoding a 205 amino acid ORF corresponding to a translated product of 23 kD different from the previous HPO that lacks the N-terminal 80 amino acids, was isolated from human fetal liver cDNA library by 5′ RACE methods.
     利用 5′RACE技术从人胎肝组织中分离一种新形式的肝细胞生成素 (HPO 2 0 5 )cDNA ,其编码蛋白质氨基酸序列的N端较已报道的人肝细胞生成素HPO(hepatopoietin)多 80个氨基酸 ,推测其蛋白质分子量为 2 3kD。
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     Methods Clone the gene encoding variable region of immunoglobulin from murine hybridoma cells by RT-PCR and RACE methods. Construct human-mouse chimeric antibody by fusing the cloned gene with the gene encoding the constant region of human immunoglobulin.
     方法采用RT-PCR及RACE技术从鼠杂交瘤细胞克隆免疫球蛋白可变区基因,与人免疫球蛋白恒区基因拼接,构建人-鼠嵌合抗体基因,转染CHO细胞并测定其表达量。
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     Secondly,5'-and 3'-end of the cDNA was cloned through RACE methods. Finally,fragments were assembled and analyzed.
     通过5'-RACE和3'-RACE技术进行cDNA末端的快速扩增,成功地克隆了pds的全长cDNA。
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  “race methods”译为未确定词的双语例句
     According to the GZ-ACO cDNA fragment sequence,full length of GZ-ACO cDNA was generated by 5′RACE and 3′RACE methods.
     根据该cDNA片段序列,设计两对末端扩增的特异引物,利用RACE-PCR技术,获得GZ-ACO片段的5′端和3′端序列。
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     By bioinformatical analysis, RT-PCR and RACE methods, we have identified a novel hKCNE4 gene from human cDNA library.
     本研究应用生物信息学分析 ,通过RT PCR和RACE方法 ,从人类心脏cDNA文库中获取人类hKCNE4全长cDNA序列。
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     Cloning of a Pathogenesis-related Protein Gene cDNA of Potato Using RACE Methods Combined with cDNA Library
     cDNA文库与RACE方法结合克隆一个马铃薯病程相关蛋白基因cDNA(英文)
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     Methods Firstly, the complete genomic sequence of the FJ11 virus strain was tested by using RT-PCR and 5′, 3′ terminal RACE methods. Then the sequence of the virus was compared with that of other dengue-2 type viruses by using Clustal method of DNASTAR software, and the phylogenetic relation analysed.
     方法 采用RT PCR和 5′、3′端RACE方法测定病毒基因组的全序列 ,并运用DNASTAR软件的Clustal法将该毒株的序列与其它 6株登革 2型病毒进行比较 ,分析其进化关系。
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     1. A full cDNA sequence of ferritin gene was cloned from leaves of Malus domestica Borkh. cv 'Gala' by RT-PCR and RACE methods.
     1.根据已报道植物铁蛋白基因(ferritin)的保守区域设计引物,用RT-PCR和RACE的方法从‘嘎拉’苹果(Malus domestica Borkh.cv‘Gala’)叶片中获得苹果铁蛋白基因(MdFer)的全长序列。
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  相似匹配句对
     Methods
     方法和对象
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     Methods
     研究方法:
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     The race differentiation was determined.
     测定了黄萎病菌的生理小种分化;
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     Auto Race in China
     企业借汽车运动造势
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     Avoidance of critical race
     临界竞争的消除方法
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  race methods
The reverse transcriptase-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) methods were employed to synthesize the cDNA fragments.
      
Sucrose: sucrose 1-fructosyltransferase (1-SST) cDNA from Lactuca sativa, coding the enzyme responsible for lower degree polymers fructan biosynthesis, was cloned by RT-PCR and RACE methods.
      
A cDNA encoding spinach α-glucosidase was cloned and sequenced bythe reverse-transcription polymerase chain reaction (RT-PCR) and rapidamplification of cDNA ends (RACE) methods.
      
A new full-length cDNA (Ssd12) encoding a Δ12-fatty acid desaturase (Δ12-FAD) was cloned from Sapium sebiferum using RT-PCR and RACE methods.
      
In this study, a DREB homolog gene, DvDREB2A, was isolated from chrysanthemum (Dendranthema vestitum) by reverse transcriptase-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) methods.
      
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Objective: cDNA Cloning and sequencing of the 5′ end gene of hepatitis G virus (HGV) from Chinese. Method: Modified rapid amplification of cDNA ends (RACE) method. The 5′ end gene of HGV was amplified from a HGV positive blood donor from Gu′an, Hebei Province. The gene was cloned into M13mp18 vector for DNA sequencing. Four clones were sequenced. Results: No identical sequences to be found in the four clones and the sequences had 74-34% homology with that reported. Conclusion: The results suggested...

Objective: cDNA Cloning and sequencing of the 5′ end gene of hepatitis G virus (HGV) from Chinese. Method: Modified rapid amplification of cDNA ends (RACE) method. The 5′ end gene of HGV was amplified from a HGV positive blood donor from Gu′an, Hebei Province. The gene was cloned into M13mp18 vector for DNA sequencing. Four clones were sequenced. Results: No identical sequences to be found in the four clones and the sequences had 74-34% homology with that reported. Conclusion: The results suggested the existence of diversity in the 5′ end gene of HGV.

目的:克隆中国人庚型肝炎病毒5′端基因,并对其cDNA序列进行分析。方法:应用改进的cDNA末端快速扩增法,即利用特异引物合成第一链cDNA,纯化后在其3′端加poly(A)“多聚尾”,用Oligod(T)-anchor等引物进行“巢式”PCR扩增,从河北省固安县一献血员血清中获得HGV5′端基因片段,与相应载体连接后转化宿主菌,随机挑选4个阳性克隆进行序列分析。结果:4个克隆的基因序列均有差异,且与3株美国HGV的5′最末端都存在一定变异。结论:中国人庚型肝炎病毒5′端存在基因多样性,提示庚型肝炎病毒的5′非编码区并不是很保守。用此方法克隆单链RNA病毒基因组的5′端,国内尚未见报道

Objective:To determine 5′ and 3′ noncoding region sequences of a new dengue 2 virus FJ 10 strain and to analyze the relationship between the secondary structure and the functions. Methods:Total RNA was extracted from a suckling mouse brain infected by the dengue 2 virus FJ 10 strain. Following the reverse transcription, the cDNAs of both 5′ and 3′ termini were amplified using RACE method, respectively. The cDNAs were cloned into the pGEM T Easy vector and sequenced, and the secondary structures...

Objective:To determine 5′ and 3′ noncoding region sequences of a new dengue 2 virus FJ 10 strain and to analyze the relationship between the secondary structure and the functions. Methods:Total RNA was extracted from a suckling mouse brain infected by the dengue 2 virus FJ 10 strain. Following the reverse transcription, the cDNAs of both 5′ and 3′ termini were amplified using RACE method, respectively. The cDNAs were cloned into the pGEM T Easy vector and sequenced, and the secondary structures of the 5′ and 3′ termini were predicted with the RNAdraw software. Results and Conclusions: The 5′ and 3′ noncoding regions comprise 96 and 454 nucleotides, respectively , and are predicted to fold into complicated stem loop structures which are possibly correlated with viral replication and virulence.

目的 :测定新分离登革 2型病毒福建 (FJ 10 )株 5′和 3′端非编码区序列 ,分析二级结构与功能的关系。方法 :从FJ 10株感染的乳小鼠脑中提取总RNA ,利用RACE法分别扩增 5′和 3′端cDNA片段 ,并克隆至pGEM TEasy载体中 ,然后分别测定插入片段的核苷酸序列 ,并用RNA绘图软件预测 5′和 3′端非编码区的二级结构。结果和结论 :FJ 10株 5′和 3′端非编码区长度分别为 96和 4 54个核苷酸 ,并形成复杂的茎环结构 ,可能与病毒的复制和毒力有关。

Objective To analize the sequences of 5′ and 3′ terminal region of dengue type 2 virus 04 strain. Methods Total RNA was isolated from dengue type 2 virus 04 strain (D2 04) infected C6/36 cells. With this RNA as templete, the cDNA of both 5′ and 3′ termini of D2 04 were amplified using RACE method respectively. The cDNAs were separately inserted into pGEM T vector and the recombinant plasmids containing the 5′ terminus 535 bp and 3′ terminus 503 bp were obtained. The nucleotide sequence of the...

Objective To analize the sequences of 5′ and 3′ terminal region of dengue type 2 virus 04 strain. Methods Total RNA was isolated from dengue type 2 virus 04 strain (D2 04) infected C6/36 cells. With this RNA as templete, the cDNA of both 5′ and 3′ termini of D2 04 were amplified using RACE method respectively. The cDNAs were separately inserted into pGEM T vector and the recombinant plasmids containing the 5′ terminus 535 bp and 3′ terminus 503 bp were obtained. The nucleotide sequence of the inserted cDNA fragment were determined. The nucleotide sequences of the 5′ and 3′ noncoding regions of D2 04 were compared with other Dengue type 2 viruses such as JAM、NGC、S1、16681 and PDK 53. Results The results showed that they shared 98 96%,98 96%,93 75%,98 95%,97 92% and 97 72%,97 80%,90 65%,94 26%,94 22% homology with D2 04 strain respectively. Conclusion Except S1, the homology between D2 04 and other type 2 viruses was higher than 94%.

目的 测定登革 2型病毒 0 4株 (D2 0 4)基因组 5′和 3′末端序列。方法 从D2 0 4感染的C6 / 36细胞中提取总RNA ,以该RNA为模板 ,利用RACE法 ,分别扩增D2 0 4株的 5′和 3′末端cDNA片段。将其分别与 pGEM T载体连接得到含有 5′端 5 35bp和 3′端 5 0 3bpcDNA的重组质粒 ,并测定上述cDNA插入片段的序列。将D2 0 4的 5′和 3′端非编码区的核苷酸序列与其它登革 2型毒株进行同源性比较。结果 D2 0 4株与JAM、NGC、S1、16 6 81和PDK 5 3株的同源性分别为98 96 % ,98 96 % ,93 75 % ,98 95 % ,97 92 %和 97 72 % ,97 80 % ,90 6 5 % ,94 2 6 % ,94 2 2 %。结论 D2 0 4株除与S1株的同源性略低外 ,其余株的同源性均在 94%以上。

 
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