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rat ovary
相关语句
  大鼠卵巢
    Comparative Study of HCG Receptors in Rat Ovary and Testis
    大鼠卵巢、睾丸人绒毛膜促性腺激素受体的比较研究
短句来源
    The roles of matrix metalloproteinases 2, 9 at the corpus luteum in the rat ovary
    基质金属蛋白酶-2、-9在大鼠卵巢黄体中的作用
短句来源
    GABA inhibits progesterone production induced by hCG in luteal cells of rat ovary
    GABA抑制hCG诱发大鼠卵巢黄体细胞孕酮生成
短句来源
    Objective To study the roles of matrix metalloproteinases 2, 9(MMP 2, 9) in the tissue remodeling that occurs during luteal development and regression in the rat ovary.
    目的 探讨基质金属蛋白酶 2、 9(MMP 2、 9)在大鼠卵巢黄体发育、维持和退化的组织重建中的作用。
短句来源
    Methods MMP 2, 9mRNAs expressions at the corpus luteum in the pseudopregnant rat ovary were assessed by in situ hybridization, and the results were determined by semi-quantitative analysis through densitive scanning method.
    方法 采用原位杂交 (insituhybridization)方法检测假孕大鼠卵巢黄体中MMP 2、 9mRNAs的基因表达变化 ,并通过密度扫描方法对原位杂交结果进行半定量分析。
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  “rat ovary”译为未确定词的双语例句
    Simultaneous Detection of 17α-hydroxylaseand Luteinizing Hormone Receptor( LHR)mRNA from Rat Ovary by Ribonuclease Pro-tection Assay(RPA).
    RNase保护法同时检测黄体生成素受体及17α-羟化酶的mRNA
短句来源
    VARIATION OF GELATINASES ACTIVITY IN RAT OVARY DURING ESTROUS CYCLE AND EARLY PREGNANCY
    大鼠动情周期及妊娠早期卵巢中明胶酶A活性变化
短句来源
    Expression and regulation of PACAP mRNA during periovulation in the rat ovary
    PACAP mRNA在大鼠排卵过程中的表达与调节
短句来源
    Objective To study the roles of matrix metalloproteinases 2, 9(MMP 2, 9)in the tissue remodeling that occurs during follicular development in the rat ovary.
    目的 探讨基质金属蛋白酶 2 , 9(MMP 2 , 9)在大鼠卵泡发育的组织重建中的作用。
短句来源
    Methods MMP 2, 9mRNAs expressions during estrous cycle in the rat ovary were assessed by in situ hybridization,and the results were determined by semi quantitative analysis through densitive scanning method.
    方法 采用原位杂交方法检测动情周期大鼠卵泡中MMP 2 , 9mRNAs的基因表达变化 ,并通过密度扫描方法对原位杂交结果进行半定量分析。
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  rat ovary
Response of the infantile rat ovary to intrasplenic transplantation into adult castrated diabetic animals
      
Regeneration of the rat ovary after repeated resection
      
Denervation of the rat ovary by removal of the semilunar ganglia of the solar plexus disturbs the estrus cycle; it increases to 10-12 days, and there are abnormalities of the smears caused by disturbed growth of the vaginal epithelium.
      
Regenerative capacity of rat ovary after compensatory hypertrophy
      
Since Par-4 expression appears to be ubiquitous we investigated the possibility of Par-4 having a role in the rat ovary granulosa cells apoptotic death.
      
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It has been documented that the ovariancell membrane has specific HCG receptors.The isolation and characterization of theovarian cell membrane have been studied. Rat ovaries are homogenized and sonicedunder ice-cold conditions. The homogenatesare centrifuged at 2000×g for 15 min. Aftersucrose density gradient ultracentrifugation(78000×g, 4h), two fractions are collectedfrom layer of 33% and interface between 33%and 37%. The membrane layer is taken outand washed free of sucrose by centrifugationat 39000×g...

It has been documented that the ovariancell membrane has specific HCG receptors.The isolation and characterization of theovarian cell membrane have been studied. Rat ovaries are homogenized and sonicedunder ice-cold conditions. The homogenatesare centrifuged at 2000×g for 15 min. Aftersucrose density gradient ultracentrifugation(78000×g, 4h), two fractions are collectedfrom layer of 33% and interface between 33%and 37%. The membrane layer is taken outand washed free of sucrose by centrifugationat 39000×g for 1h. The pellets are suspendedin an appropriate: amount of 40 mM Trisbuffer. In comparison with homogenates andsonicates, the activity of 5-nucleotidase ofplasma membrane increases ten times. HCGreceptor binding of the plasma membrane is1750 times as high as the homogenates andsonicates. Preparations are checked by elec-tron microscopy. Nuclei, mitochondria,lysomes, rough microsomes and Golgimembranes are absent from most of thepreparations. The separation at the four sucrose con-centrations (33%, 37%, 41%, 45%) is more effec-tive than at the five sucrose concentrations(33%, 37%, 41%, 45%, 48%). The centrifugespeed (78000×g) is the optimum speed.

作者用匀浆、超声波及蔗糖密度梯度离心法制备了卵巢细胞膜,较单纯匀浆之HCG结合率提高1750倍,四种蔗糖浓度及五种蔗糖浓度之分离效果对比,以前者为宜,离心速度为78000×g时结合率最高,且集中于33%表面层及33%~37%界面层。

It has been documented that there arespecific human chorionic gonadotropin(HCG)receptors in cell membrane of rat ovary and testicle. In or-der to make a further study on the Characteristics ofthe HCG receptors on rat ovary and testicle,we fo-cused on comparing those two kinds of receptors. Fur-thermore,we contrasted the testicular cell membranewith its homogenates,Rat ovarian and testicles werehomogenized and soniced under ice cold conditions. The homogenates were centrifuged at 12 100 ×g for...

It has been documented that there arespecific human chorionic gonadotropin(HCG)receptors in cell membrane of rat ovary and testicle. In or-der to make a further study on the Characteristics ofthe HCG receptors on rat ovary and testicle,we fo-cused on comparing those two kinds of receptors. Fur-thermore,we contrasted the testicular cell membranewith its homogenates,Rat ovarian and testicles werehomogenized and soniced under ice cold conditions. The homogenates were centrifuged at 12 100 ×g for 20min following filtration. After sucrose density gradi-ent ultracentrifugation(78 000×g,2.5h) for the pel-lets,the interface layers between 37%and 4 1%werecollected. The pellets were subsequently sustJended incold1mmol/L NaHCO_3 solution and recentrifuged at 27 000×g for 25 min.Theresults show that the cell membrane of boththe rat ovary and testicle can bind to HCG specifically.Asmall amount of HCG has more evident inhibitoryeffect on the binding in testis than ovary.Scatchard plot analysis for the binding assayyielded a bound capacity of testicle cell membrane of4.5 × 10 ̄(-5)mol/μg protein,Kd of 1.6 × 10 ̄(-7)mol/L,whereas it was 0.51 × 10 ̄(-9) mol/μg protein,a Kd of1.69× 10 ̄(-6)mol/L in ovarian cell membrane.In comparison with homogenates and cell mem-brane, the HCG binding activity of later is more sensi-

以分离的细胞膜为受体制剂,对大鼠卵巢及睾丸人绒毛膜促性腺激素(HCG)受体进行比较研究,发现二者的受体结合特性有一定差异;同时将大鼠寒丸膜及睾丸匀浆作对比,友现睾丸膜制剂较睾丸匀浆更能准确地体现睾丸HCG受体的真正特性。

Objective :To determine the effect of endothelin-l on the progesterone( P)ecretion by rat luteal cells and its relation with cAMP. Design :In vitro experimental study. Setting:Laboratory in Shanghai lnstitute of Planned Parenthood Research. Methods:Preparation of luteal cells suspension from Sprague-Dawley rat ovaries and incubation in KR-BGA1 solution,Samples of incubation medium were collected and stored at-20℃for measurement of Pand cAMP with radioimmunoassay. Results:Endothelin-l at a concentration...

Objective :To determine the effect of endothelin-l on the progesterone( P)ecretion by rat luteal cells and its relation with cAMP. Design :In vitro experimental study. Setting:Laboratory in Shanghai lnstitute of Planned Parenthood Research. Methods:Preparation of luteal cells suspension from Sprague-Dawley rat ovaries and incubation in KR-BGA1 solution,Samples of incubation medium were collected and stored at-20℃for measurement of Pand cAMP with radioimmunoassay. Results:Endothelin-l at a concentration of l0-6mol/L significantly reduced the basal and hCG inducedP secretion about 20%and29%respectively (P<0. 0l ).xogenous cAMP significantly increased the p se-cretion to about 22%of the control group(P<0.05).When added to the endothelin-1 treated medium theP secretion returned to the control level. Forskolin stimulated the P secretion to 2.l times higher than thecontrol (P<0. 0l ),and when added to the endothelin-l culture system,the P secretion increased to a levelhigher than the control (P<0.01).The cAMP concentration in the luteal cell mediuM was significantly re-duced(P<0.05)when culltured with endothelin-l. Conelusion :The study suggested that endothelin-1 may be an intra- ovarian regulatory factor which in-hibits progesterone secretion from rat luteal cells by suppressing the adenyl cyclase activity and reducingcAMP accumulation .

本研究采用大鼠黄体细胞体外孵育技术,观察了缩血管肽内皮素-1(ET-1)对黄体细胞孕酮分泌的影响及其与细胞内信使cAMP的相互关系。结果表明,10-6mol/L的ET-l可显著抑制黄体细胞的基础孕酮分泌和hCG诱导的孕酮分泌;外源性cAMP和腺苷酸环化酶激动剂Forskolin则可促进黄体细胞分泌孕酮;当cAMP或Fonskolin与ET-1共同作用时,前者可完全反转ET-1对黄体细胞的抑制作用,孕酮分泌恢复到对照组水平。提示El-1可能是一种卵巢局部调节因子,通过抑制黄体细胞内的腺苷酸环化酶,减少cAMP的生成和释放,进而抑制黄体细胞的孕酮分泌。

 
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