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  renaturation
     Renaturation and purification of IL-6 D24-PE40KDEL, a novel recombinant IL-6 Pseudomonas exotoxin fusion protein
     新型重组IL-6D24-PE40KDEL外毒素融合蛋白复性及纯化
短句来源
     The expression, renaturation, purification and preliminary identification of human IgE Cε3-Cε4
     人IgE Cε3-Cε4蛋白的表达、复性、纯化及初步鉴定
短句来源
     Study on Purification and Renaturation of His6-γTNFβFusion Protein (IB)
     人His6-IFNγ/TNFβ(His6γ-TNFNβ)融合蛋白(包涵体)的分离、纯化与复性研究
短句来源
     Further investigation demonstrated that 3F1 is ideal for affinity purification of rHuIFN α2a, resulting from the recovery rate of 0.8 mg ̄1.0 mg of rHuIFN α2a per ml affinity gel with per ml affinity gel the purity of more than 95% and activity of 2×10 11 u/g, and beneficial to the renaturation of rHuIFN α2a.
     进一步研究发现,mAb3F1具有良好的用于亲和层析纯化rHuIFNα2a的性能,每毫升湿胶可回收0.8mg1.0mgrHuIFNα2a,纯度达95%以上,生物学活性达2×1011u/g,且mAb3F1有助于rHuIFNα2a的复性
短句来源
     Results SDS PAGE and Western blot showed that HPV16 L1(m202) protein had been expressed in sf9 cells, the efficiency of purification and renaturation was approximately 17%, the anti serum has the ability of competing inhibition HPV16 McAb reacting with HPV16 L1(m202) protein.
     结果 SDS PAGE、western blot结果证明HPV16L1(m2 0 2 )蛋白的表达 ,纯化复性后产率约为 17% ,免疫血清具有竞争抑制HPV中和单抗与HPV16L1(m2 0 2 )相结合的能力。
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  refolding
     Refolding and Purification of the huGM-CSF(9-127)-IL-6(29-184) Fusion Protein
     huGM-CSF(9-127)-IL-6(29-184)融合蛋白的复性及纯化研究
短句来源
     Purification and Refolding of HIV-1 p24 Protein
     HIV-1Gagp24蛋白的纯化及复性
短句来源
     Expression, Purification and Refolding Study of Single Chain Antibody 2F3
     单链抗体ScFv2F3的表达纯化及复性研究
短句来源
     Expression and refolding of a HLA-A*0203-BSP fusion protein and identification of its tetramers
     HLA-A*0203-BSP的表达和复性及其四聚体的鉴定
短句来源
     Refolding research of recombinant human osteogenic protein-1(rhOP-1)
     重组人成骨蛋白-1(rhOP-1)的复性
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  renature
     the recovery yield of pQE30-B is 30% and the renature of pQE40-B was failure.
     ()采用谷耽甘肽再氧化法对 pQE30BZ和 pQE40-B3表达的包涵体进行柱后复性
短句来源
     3,The inclusion bodies were dissolved by 6 mol/L guanidine hydrochloride and 50 mmol/L P -mercaptoethanol,then was diluted in the buffer contained 0.5 mol/L Arg to renature for 72 h. The activity experiments verified that the denaturating protein recovered its bioactivity.
     3、包涵体蛋白以6 mol/L盐酸胍及50 mmol/L β-巯基乙醇(β-mercaptoethanol,β-ME)溶解后,于含有0.5mol/L精氨酸的缓冲液中稀释复性72h,活性实验证明复性后的蛋白质恢复了其生物活性。
短句来源
     Secondly, the monoeric rhTGF-β3 were added to a special solution containing of 1mol/L NaCl, 05mol/L l-Arg, 0.5mmol/L GSSG, 30mmol/L CHAPS and 20%(v/v)DMSO to renature. Finally,the renatured rhTGF-β3 was purified by a DEAE Sepharosel Fast Flow column and a Sephacryl S-100 column.
     将单体蛋白加到复性缓冲液(1mol/LNaCl,0.5mol/LLArg,0.5mmol/LGSSG,30mmol/LCHAPS,20%(v/v)DMSO)进行复性后,再经DEAESepharoselFastFlow柱和SphacrylS100分子筛可分离得到纯度达94%的二聚体rhTGFβ3,纯化后的产量为720mg/L,纯化总回收率为47%。
短句来源
     The GST-BoPrP(23—242) fusion proteins were collected by two ways: the first is to purify and renature from the inclusion bodies;
     将鉴定之后的重组表达菌诱导表达后,通过两种方法回收GST-BoPrP(23~242)融合蛋白:其一,是从包涵体中提取和复性GST-BoPrP(23~242)融合蛋白;
短句来源
     After denature and renature procedure in Ni~(2+) _NTA affinity chromatography column, soluble chitinase was obtained.
     在Ni2+-NTA亲和柱上经变、复性和纯化,得到可溶的几丁质酶。
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  renatured
     The optimum conditions were 5 mmol·L -1 and 0.5 mmol·L -1 for the concentrations of GSSG and GSH, respectively, and renatured time more than 48 hours at 10 ℃.
     当氧化型谷胱甘肽(GSSG)浓度为5mmol·L-1,还原型谷胱甘肽(GSH)浓度为0.5mmol·L-1,10℃复性48h以上时,复性率较高。
短句来源
     Results: The expression vector pET28aHsp65E6/E7 was constructed successfully and E6/E7 gene was mutated correctly. Hsp65E6/E7 fusion protein was renatured and purified on the affinity chromatography column simultaneously. The protein purity achieved 95% after the anionic exchange chromatograph purification.
     结果:成功构建pET28a-Hsp65-E6/E7重组表达载体,E6/E7突变位点正确,融合蛋白在亲和层析柱上正确复性和初步纯化,经阴离子交换色谱纯化后蛋白纯度达到95%。
短句来源
     RESULTS Dialysis, anion exchange chromatography and gel filtration renatured ETI with relative renaturation ratios of 20.1%, 32.1% and 50.8% and relative recoveries of protein 16.6%, 15.1% and 55.2%,respectively.
     结果  3种复性方法的复性率分别为 2 0 . 1% ,32 . 1%和 5 0 . 8% ,蛋白质回收率分别为 16 . 6 % ,15 . 1%和 5 5 . 2 %。
短句来源
     The biological activities of renatured IL13 fusion protein could reach 80~120 U/ml and the fusion proteins could be cut into 26 kD GST and 10 kD IL13 with the treatment of thrombin for several hours. 
     复性后IL13融合蛋白的生物学活性可达80~120U/ml。 复性后的融合蛋白经凝血酶作用后,可被切割成26kD的GST与10kD的hIL13。
短句来源
     RESULTS The final concentration of fibrin in separated gel was 0.4×10~(-3) mg·L~(-1). After electrophoresis, zymogram was obtained when the gel was renatured with 2.5% TritonX-100 for 30 min, incubated with PBS buffer(pH 7.2) for 30 min, stained with Comassie Brilliant Blue R-250, restained with 7% acetic acid.
     结果分离胶中纤维蛋白终质量浓度为0.4×10~(-3)mg·L~(-1),电泳后胶板用2.5%TritonX-100复性30min,PBS缓冲液(pH7.2)保温30min,考马斯亮蓝R-250染色,7%醋酸脱色,可以得到清晰的酶谱。
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  renaturation
fischeri luciferase reaches 80% of the initial level over several minutes, whereas renaturation of thermostable "slow" Ph.
      
luminescens luciferase proceeds substantially slower (the degree of renaturation reaches only ~7-8% of the initial level over tens of minutes).
      
Antibody-assisted folding may enhance renaturation of recombinant proteins from inclusion bodies.
      
Antibody-assisted folding may enhance renaturation of recombinant proteins from inclusion bodies.
      
Renaturation, activation, and practical use of recombinant duplex-specific nuclease from Kamchatka crab
      
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  refolding
Studies of Peroxidase Refolding in the Presence of Specific Antibodies
      
The yield of the active enzyme in the course of the refolding was increased by 1.5-1.7 times in the presence of antibody H1.
      
The refolding experiments after denaturation of proteins by guanidine hydrochloride or urea have been also analyzed.
      
It was shown that aggregation accompanying refolding follows first order kinetics at the final phase of the process.
      
The model of protein refolding explaining such a kinetic regularity has been proposed.
      
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  renature
Thermally denatured DNA of 11 species of Cichorieae (Compositae) was allowed to renature at 69° C in 0.8 M Na+.
      
Interstand cross-links have now been found in molecules of all sizes by examining the ability of high molecular weight DNA to snap back, i.e., to rapidly renature after denaturation.
      
It was possible to renature the enzyme by passing it through an anion exchange column.
      
To confirm whether the APase is active in the monomeric form, we attempted to elute the enzyme from SDS-polyacrylamide gels with Disk electrophoresis apparatus and renature the enzyme.
      
Water-in-oil (w/o) microemulsion of sucrose fatty acid ester was used to renature denatured hen egg white lysozyme without aggregation.
      
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  renatured
Immunochemical properties of recombinant A-subunit expressed in Escherichia coli and renatured were investigated using a panel of monoclonal antibodies raised against three mistletoe toxins (MLI, MLII, and MLIII).
      
The recombinant protein PKPI-B10 obtained as inclusion bodies was denatured, separated from admixtures by ion-exchange fast protein liquid chromatography (FPLC) on MonoQ under denaturing conditions, and renatured.
      
Although the oxidized and reduced glutathione (GSSG and GSH) were employed in the oxidized mobile phase, the reduced-denatured insulin still could not be renatured.
      
The three disulfide bridges of insulin were formed correctly and the reduced-unfolded insulin can be renatured completely.
      
The renatured protein purified by Ni-NTA Superflow resins showed ability to bind to antigen on SPC-A-1 lung adenocarcinoma.
      
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