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baculovirus
相关语句
  杆状病毒
    Functional Analysis of the Baculovirus Chitinase Gene
    杆状病毒几丁质酶基因功能分析
短句来源
    Studies on the Improvement of Baculovirus Expression System, the Function of p74 Gene and Field Trial of Genetically Modified Baculovirus Insecticide
    杆状病毒新型表达系统及P74基因功能和杀虫剂800亩田间释放研究
短句来源
    The Expression of CAT Gene in E.coli under the Late Promotor of Insect Baculovirus
    昆虫杆状病毒晚期启动子在大肠杆菌中启动CAT基因表达
短句来源
    FUNCTIONS AND STRUCTURES OF NONESSENTIAL GENES OF BACULOVIRUS
    杆状病毒非必需基因的结构与功能
短句来源
    REPLICATION AND INFECTION PATHWAY OF BACULOVIRUS IN INSECT HOST
    杆状病毒在昆虫宿主内的感染途径及其复制
短句来源
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  “baculovirus”译为未确定词的双语例句
    The Study on Insecticides of Recombinant Baculovirus of Helicoverpa Armigera Functional Genes and the Establishment of Cell Lines from H. Armigera
    棉铃虫功能基因重组病毒杀虫剂研究及棉铃虫细胞系的建立
短句来源
    Previous results have shown that an elevation of cytchrome c in the cytosol and a corresponding decrease in the mitochondrial in the apoptotic process induced by Syngrapha falcifera multiple nuclear polyhedrosis virus(SfaMNPV)in Spodoptera litura cell line(SL-1). In this study we further investigate the influence of cytchrome c on apoptosis induced by baculovirus in insect cells.
    我们以前的研究结果已证明,在芹菜夜蛾核型多角体病毒(Syngrapha falcifera multiple nuclear polyhedrosis virus,SfaMNPV)诱导斜纹夜蛾(Spodoptera litura Cells,SL-1)细胞凋亡过程中,细胞色素c(Cytochrome c,cytc)随着诱导时间的延长,不断从线粒体释放到细胞质中。
短句来源
    Transformation of Tobacco (Nicotiana Tabacum) with Baculovirus Chitinase and Evaluation of Its Expression and Effectiveness in Improving Fungal Resistance
    几丁质酶基因在烟草中表达及对真菌病害的抗性研究
短句来源
    REPLICATION AND SERIAL PASSAGE OF A SINGLY ENVELOPED BACULOVIRUS OF TRICHOPLUSIA NI(LEPIDOPTERA:NOCTUIDAE)IN A HOMOLOGOUS HOST CELL LINE
    粉纹夜蛾核型多角体病毒在同源寄主细胞系中复制和连续传代的研究
短句来源
    Sequence Analysis of the Putative ν-cathepsin Gene of Heliothis Armigera Single-nucleocapsid Baculovirus
    棉铃虫单核衣壳核多角体病毒组织蛋白酶基因的序列分析和进化研究
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  baculovirus
The construction and preliminary analysis of a Tn5 transposon based random mutant library of baculovirus
      
A transposon-based random mutation library of AcMNPV, the type species of baculovirus, was constructed using a Tn5 transposon.
      
This technique will be very useful in the functional study of baculovirus genes.
      
Analysis of published data demonstrated that the process of baculovirus epizootic depends on a set of various factors.
      
Based on the data obtained by highly sensitive methods of molecular biology, this review summarizes the discussion of the source of viral infection in baculovirus epizootics.
      
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In 1977 this nuclear polyhedrosis virus was isolated from the dead larvae of Buzura suppressaria Guenee in the tea garden of "Wang Ling, Hunan. Under ordinary and phase contrast microscopes the polyhedra were seen as nearly rounded, rhombic, trigonal and hexangular particles and 1.2 to 3.6 ft in size. Under the electron microscope, the viron rods liberated from the polyhedra in 0.01 M Na.CO3 and 0.05 NaCl measured 49-63 nm by 251-282 nm. This virus belongs to subgroup A of Baculovirus.Oral administration...

In 1977 this nuclear polyhedrosis virus was isolated from the dead larvae of Buzura suppressaria Guenee in the tea garden of "Wang Ling, Hunan. Under ordinary and phase contrast microscopes the polyhedra were seen as nearly rounded, rhombic, trigonal and hexangular particles and 1.2 to 3.6 ft in size. Under the electron microscope, the viron rods liberated from the polyhedra in 0.01 M Na.CO3 and 0.05 NaCl measured 49-63 nm by 251-282 nm. This virus belongs to subgroup A of Baculovirus.Oral administration of the virus on leaves by the larvae of the fourth and fifth instars showed the medium lethal concentration (LC50) to be 10-4.497 (787 PIB) on the fourth day, 10-5.0 (243 PIB) on the nineth day, and 10-6.251 (7 PIB) on the eleventh day. Young larvae were more susceptible to the virus and different routes of virus infection caused different mortality rate.Field test showed the mortality rate of the larvae may reach 98% after spraying with the polyhedral suspension and some infective effect on the later generations may appear.

1977年从湖南省网岭茶园内罹病大尺蠼幼虫中分离获得大尺蠖核型多角体病毒。多角体近圆形、菱形、三角形或六方形,大小约1.2—3.6微米。电镜下观察病毒粒子为杆状(49—63毫微米×251—282毫微米)。属杆状病毒科(Baculoviridae)杆状病毒属(Baculovirus)A亚组(Subgroup A)。 对4—5龄大尺蠖室内口服的致死中浓度(LC_(40)):4天为10~(-4.497)(787个多角体),9天为10~(-5.0)(243个多角体),11天为10~(-6.321)(7个多角体)。不同感染方法感染3—4龄幼虫7其致病力不同。大田防治杀虫效果达98%,并有后效作用。

A new cell line,designated BCIRL-PX2-HNU3,derived from 20 mincedtrypsinized pupae of diamond-back moth,Plutella xylostella,has been estab-lished.Primary culture was set up in TC-199-MK medium on July 1,1982.It Wasrefed once a week.August 17,48 days after explantation,the first subculturewas made just by flushing an equal volume of fresh medium upon the confluentsheets,then the cells were dispensed into two new culture flasks.The cellsgrew very rapidly.Since then they were subcultured three times a week at asplit...

A new cell line,designated BCIRL-PX2-HNU3,derived from 20 mincedtrypsinized pupae of diamond-back moth,Plutella xylostella,has been estab-lished.Primary culture was set up in TC-199-MK medium on July 1,1982.It Wasrefed once a week.August 17,48 days after explantation,the first subculturewas made just by flushing an equal volume of fresh medium upon the confluentsheets,then the cells were dispensed into two new culture flasks.The cellsgrew very rapidly.Since then they were subcultured three times a week at asplit ratio 1∶2 and were subcultured at a ratio 1∶10 when they reached theirpassage 100.Now,this line is in their 115 passage.BCIRL-PX2-HNU3 cells were not firmly attached and floating cells werealways observed in the medium.Most cells of this line were round and oval inshape,some were elongated or spindle shaped,although there were some othertypes of cells in the mixed cell population.The population doubling time at 28℃ in 96 hours was 30±2.06 and thecell density reached,from 1×10~5 cells/ml inoculated,a level of approximately1.3×10~6 cells/ml after 8 days.Chromosomes were typical of other lepidopteran cells.BCIRL-PX2-HNU3 was distinguished from cell lines derived from otherinsect species by isoelectric focusing using the enzyme phosphoglucoisomerase.Preliminary studies indicated that BCIRL-PX2-HNU3 cells could supportthe replication of some baculoviruses.The inclusion bodies appeared in thenucleus of the cells within 48hrs after challenging the culture with Autogra-pha californica's NPV.Inclusion bodies of VHA-273,another heterologousbaculovirus isolated from Heliothis armigera in China could be replicated inPlutella cells as well,either challenged the cells with infectious hemolymphor infectious supernatant from infected cultures.One more interestingthing was that 6 days after challenging the cells with infectious hemolymphof its homologous baculovirus,Plutella granulosis virus,GV capsules werefound in the cytoplasm of cultured Plutella cells,and it was confirmed byEM pictures.But we failed to get it reconfirmed in subsequent experiments.

第一株小菜蛾(Plutella xylostella)蛹发展起来的细胞系已经建成并命名为BCIRL-PX2-HNU3.BCIRL-PX2-HNU3是由20个剪碎的全蛹经过消化处理,培养在TC-199-MK 培养基中建立起来的.原代培养只历时48天就开始了第一次转代,由于细胞增殖快而稳定、此后即按1∶2的分裂比值,每周转代三次,待转代100次后,改按1∶10的比值转代,每周一次,至目前为止,该细胞已传至115代.BC1RL-PX2-HNU3细胞仅疏松贴附瓶壁,容易从瓶壁脱落.转代时,不需用消化酶.细胞的形状多种多样,但以园形和卵园形为主.28℃时,96小时细胞群体倍增时间为30小时±2.06.1×10~5/毫升细胞培养8天后,最大密度可达1.3×10~6细胞/毫升.该细胞系细胞的染色体粗短,多为多倍体,具有鳞翅目昆虫染色体的典型性状.用磷酸葡萄糖异构酶作等电聚焦,该细胞系与一些其他细胞系有明显差异.试验表明:BC1RL-PX2-HNU3细胞系对苜蓿尺蠖(Autographa californica)的核型多角体病毒以及中国棉铃虫(Heliothis armigera)病毒VHA—273敏感,并能复制出多角体.另...

第一株小菜蛾(Plutella xylostella)蛹发展起来的细胞系已经建成并命名为BCIRL-PX2-HNU3.BCIRL-PX2-HNU3是由20个剪碎的全蛹经过消化处理,培养在TC-199-MK 培养基中建立起来的.原代培养只历时48天就开始了第一次转代,由于细胞增殖快而稳定、此后即按1∶2的分裂比值,每周转代三次,待转代100次后,改按1∶10的比值转代,每周一次,至目前为止,该细胞已传至115代.BC1RL-PX2-HNU3细胞仅疏松贴附瓶壁,容易从瓶壁脱落.转代时,不需用消化酶.细胞的形状多种多样,但以园形和卵园形为主.28℃时,96小时细胞群体倍增时间为30小时±2.06.1×10~5/毫升细胞培养8天后,最大密度可达1.3×10~6细胞/毫升.该细胞系细胞的染色体粗短,多为多倍体,具有鳞翅目昆虫染色体的典型性状.用磷酸葡萄糖异构酶作等电聚焦,该细胞系与一些其他细胞系有明显差异.试验表明:BC1RL-PX2-HNU3细胞系对苜蓿尺蠖(Autographa californica)的核型多角体病毒以及中国棉铃虫(Heliothis armigera)病毒VHA—273敏感,并能复制出多角体.另外,用小菜蛾颗粒体病毒感染该细胞系,6天后,电镜切片证实培养的细胞胞质内出现了颗粒体病毒的蒴伏体,但在随后的多次试验中,未能重复.

The occurrence and epizootiology of the baculovirus disease of the bag-worm, Crypiothelea variegata Snellen, were studied by extensive census in the suburbs of Shanghai and several provinces. This disease showed a low, but persistent incidence throughout the bagworm population. Dead or moribund larvae infected by the virus were collected and used singlely or in conjunction with a Bacillus thuringiensis preparation to control the bagworm. These preparations had an efficacy comparable with the conventional...

The occurrence and epizootiology of the baculovirus disease of the bag-worm, Crypiothelea variegata Snellen, were studied by extensive census in the suburbs of Shanghai and several provinces. This disease showed a low, but persistent incidence throughout the bagworm population. Dead or moribund larvae infected by the virus were collected and used singlely or in conjunction with a Bacillus thuringiensis preparation to control the bagworm. These preparations had an efficacy comparable with the conventional chemical pesticides and were quite promising.For the present the mass production of the virus remains to be solved because the host insect is not compatible with rearing in the laboratory.

本试验用大袋蛾杆状病毒病制剂单独或与苏云金杆菌制剂混用,防治大袋蛾幼虫,兼治刺蛾幼虫。其效果相当于化学杀虫剂,有应用前途。

 
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