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tf
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     The method of tf.
     在义项矩阵的计算中,采用改进了的tf.
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     Before,the TF.
     以往只是简单地将面向文本集的TF.
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  tf
The existence of the singular integral ∫K(x, y)f(y)dy associated to a Calderón-Zygmund kernel where the integral is understood in the principal value sense TF(x)=limε→0+∫|x-y|>amp;gt;εK(x, y)f(y)dy has been well studied.
      
The note proves that if f(z)∈NH (Δ), (or AH (Δ)| f(z)|?1, ? z∈Δ then |f'(T)|?(1-|T|2)-1|I-f*(T)f(T)|1/2 |I-f(T)f*(T)|1/2 , where T?-(H) (or T*T=TT*, respectively), |T|>amp;lt;1, Tf=fT.
      
It Tf (xo ) exists for a single point xo then Tf(x) exists everywhere for x?Rn and TF?Lipα(Rn).
      
Meanwhile, the accumulation of As in the roots was greater than that in the shoots, the translocation factor (TF) was ?0.3 and the bioaccumulation factor (BF) was ?0.6, thus showing that Rorippa globosa had an excluding effect on As uptake.
      
It was identified to be 100% similar to Acidithiobacillus ferrooxidans strain Tf-49 based on 16S rDNA sequence analysis.
      
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Transfer factor (TP) is a dialysable (ultrafilterable) extract of sensitised leukocytes. It transfers cellular immunity from a skin test positive donor to a skin test negative recipient. Currently TF is recognized as one of the most potent immunological reagents. In this paper the detailed method for preparation of transfer factor from normal blood bank donors has been described and some physico-chemical and biological properties of the TF preparations have been examined. The production of TF...

Transfer factor (TP) is a dialysable (ultrafilterable) extract of sensitised leukocytes. It transfers cellular immunity from a skin test positive donor to a skin test negative recipient. Currently TF is recognized as one of the most potent immunological reagents. In this paper the detailed method for preparation of transfer factor from normal blood bank donors has been described and some physico-chemical and biological properties of the TF preparations have been examined. The production of TF is briefly as follows: After separation of the plasma (for plasma products), the buffy coats are harvested and pooled. The contaiminating red blood cells are disrupted by tris buffered or isotonic NH_4Cl solution. The pooled leukocytes having been washed iwice with cold normal saline are then alternately frozen and thawed ten times with dry ice in acetone and a 37℃ water bath. The cell lysate is placed in dialysis tubing and dialysed in the cold for 36~48 hours against running pyrogenfree deionized water. The dialysate is lyophilized. The yellowish white powder is saved and redissolved in the desired volume of pyrogen-free deionized water, and passed through a Seitz filter (EKS). 2ml of the sterile TF solution (equivalent to the dialysate of 4×10~8 leukocytes) are placed in each ampoule. The final product is stored at -20℃.The TF preparation is protein-free as determined by protein precipitating reagents. It contains ca. 148μg of peptides and 14μg of ribose per mg of dry powder. The ultraviolet absorption curve gives a peak at 250~251nm. Adenine, guanine and uracil are present in the paper chromatogram of the TF acid hydrolysate. Silica- gel thin layer chromatography reveals four spots stained with ninhydrin. Sephadex G-25 filtration gives reproducibly a characteristic elution pattern yielding 3 main peaks and several small peaks with many of the eluted peaks beyond the total volume of the column. The results of animal experiments show that the TF preparations are non-toxic, non-anaphylactic and non-antigenic.So far over 160 patients (variously afflicted with herpes zoster, chronic hepatitis B, systemic lupus erythematosus, primary carcinoma of the liver, carcinoma of the lung and leukemia) have been treated with the TF preparations. The results will be published elsewhere.

转移因子是致敏白细胞中的可透析物质,能将一个有免疫能力的人的某些细胞免疫力转移给无免疫力的人。所以供给转移因子可能是一种有效的免疫治疗措施。本文详细介绍综合利用血源制备正常人转移因子的方法,并分析其某些理化性质和生物学性质。收集分离血浆后的白细胞层,用三羟甲基氨基甲烷缓冲的或等渗的氯化铵溶液破坏其中污染的红细胞,白细胞经生理盐水洗涤后进行10次冻融,白细胞匀浆对流动去离子水透析,透析液冷冻干燥、除菌分装。每安瓿2毫升转移因子注射液相当4×10~8个白细胞的透析物,保存于-20℃备用。本制剂蛋白质定性检查阴性;每毫克干粉约含多肽148微克、核糖14微克;在250~251毫微米有吸收高峰;转移因子酸水解液硷基纸层析有三种硷基;硅胶薄板层析呈四个茚三酮显色点;葡聚糖G-25凝胶过滤呈可重复的特征性洗脱图谱,具有3个主要峰及若干个小峰,主要洗脱部分在V_t之后。动物实验表明此制剂无毒性、无过敏性和抗原性。本制剂已用于临床治疗带状疱疹、系统性红斑狼疮、慢性乙型肝炎、原发性肝癌、肺癌及白血病等一百六十余例,结果将另文发表。

An improved method for the preparation of transfer factor from human leukocytesis herein described.Pooled leukocytes were disintegrated in cold 40% ethanol with ahigh speed waring blender fitted with a perforated stainless steel cylinder.Thehomogenate was spun in a refrigerated centrifuge,and the supernatant fluid obtainedwas then lyophilised.The dry powder was dissolved in a desired volume of pyrogen-freedeionised water and the solution was then passed through an ultrafiltration membrane(impermeable to cytochromec)to...

An improved method for the preparation of transfer factor from human leukocytesis herein described.Pooled leukocytes were disintegrated in cold 40% ethanol with ahigh speed waring blender fitted with a perforated stainless steel cylinder.Thehomogenate was spun in a refrigerated centrifuge,and the supernatant fluid obtainedwas then lyophilised.The dry powder was dissolved in a desired volume of pyrogen-freedeionised water and the solution was then passed through an ultrafiltration membrane(impermeable to cytochromec)to obtain the ultrafiltrable transfer factor(TFu).Thismethod has several advantages over the dialysis procedure:(1)The procedure issimpler and strict experimental conditions can be maintained.(2)Tissue autolysis andcontamination with bacteria or pyrogen can be minimized.(3)After extraction ofTFu from the leukocytes,the residue may be used for the extraction of “immune” RNA.(4)It is suitable for large scale production of TF.Each unit of ultrafiltrable TFu so obtained was equivalent to 0.5 gm or 4.45±1.35×10~8 leukocytes,containing 6.86±0.72 mg of dry substance,183.24±26.0μgof ribose,797.22±197.07μg of polypeptides and 58.45±10.70 ng of cyclic adenosinemonophosphate.E_(250nm)=7.86±0.98,E_(260)/E_(280)=2.62±0.12.On Sephadex G-25 gelfiltration it reproducibly showed a characteristic elution pattern which was quitedifferent from that of TF_D prepared by the conventional method.The possible causesof this and other differences were briefly discussed.Our TF preparation were nontoxic,non-pyrogenic,non-anaphylaotic and non-antigenic.The delayed-type skin hypersensitivity reaction and cell-mediated immunityof patients treated with TFu were usually augmented.

本文介绍一种制备转移因子(TF)的改进方法。白细胞于冷40%乙醇溶液中被打碎,白细胞匀浆冷冻离心,上清液除去乙醇后再通过超滤薄膜,滤液即为TFu 制剂。此法操作简便,条件严格,可避免细胞内成分的自溶及细菌或热原的污染,又可综合抽提白细胞内的“免疫”核糖核酸,适合于大规模制备TF。每单位TFu 相当于0.5克或4.45±1.35×10~8白细胞的超滤物,含固体6.86±0.72毫克,核糖183.24±26.0微克,多肽797.22±197.07微克,环化腺嘌呤核苷酸58.45±10.70毫微克。E_(250毫微米)=7.86±0.98,E_(260)/E_(280)=2.62±0.12。具有特征性葡聚糖G-25凝胶过滤洗脱图谱。动物实验证明此制剂无毒性、热原、过敏性和抗原性。注射于病人能提高其迟发型皮肤变态反应及细胞免疫反应。讨论了由透析法及本法制备的TF 理化性质差别的原因。

In the paper,We show that if function f(x) satisfy:(1) f(x)∈L_M~*(G) andthe support of the function f(x) strictly belong to G,(2) for each variable x_i(i=1,…,n),f(x) has non-mixed generalized partial derivative D_i~tf(x)∈L_M~*(G) andthe Support of D_i~tf(x) strictly belong to G.then the function f(x) has.all mixedgeneralized partial derivatives of order l in L_M~*(G) and we also obtain the corre-sponding estimate.Where denote by L_M~*(G) the Orlicz space for N-function M(u)On the domain G and l is...

In the paper,We show that if function f(x) satisfy:(1) f(x)∈L_M~*(G) andthe support of the function f(x) strictly belong to G,(2) for each variable x_i(i=1,…,n),f(x) has non-mixed generalized partial derivative D_i~tf(x)∈L_M~*(G) andthe Support of D_i~tf(x) strictly belong to G.then the function f(x) has.all mixedgeneralized partial derivatives of order l in L_M~*(G) and we also obtain the corre-sponding estimate.Where denote by L_M~*(G) the Orlicz space for N-function M(u)On the domain G and l is positive integer.

本文证明了,如果函数f(x)满足:(1)f(x)∈L(?)_M(G)而且f(x)的支集严格属于G,(2)对每一个变量x_1(i=1,…,n)f(x)有非混合阶广义导致D_1(?)f(x)∈L(?)_M(G)而且D_1(?)f(x)的支集也严格属于G,则f(x)的所有l 阶混合广义导数D~af(x)在L(?)_M(G)中存在并得出了相应的估计。其中L(?)_H(G)表示由N(?)函数M(u)在区域G 上生成的Orlica 空间,而l 是正整数。

 
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