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transfer blot
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  “transfer blot”译为未确定词的双语例句
     3 antigens the 100 000g supernatant of cyst fluid (short in cyst fluid thereafter),the whole cyst and urea soluble scolex antigans were subjected to enzyme linked immunoelectro transfer blot (EITB) assay against a batch of 105 serum samples.
     选择囊液100000g(以下简称囊液)、全囊和头节尿素等3种抗原对105份各类血清进行酶联免疫电转移印斑(EITB)检测。
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     The expression of foreign MBP, BDNF or NGF gene in these neural and correlative cells driven by COLIA1 was indivadually detected using ELISA, immuno-dot blot and Western transfer blot.
     电子显微镜观察显示,相同浓度和时间处理的21 .skDa MBP及NGF、BDNF微基因转染细胞的超微结构未见明显凋亡改变。
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  相似匹配句对
     Transfer of the Prematures
     早产儿的转运
短句来源
     Study on Transfer
     给予句研究
短句来源
     Western blot;
     Western blot;
短句来源
     Applications of electrophoretic transfer, rehybridization and Biotin-probe in Southern-blot were reported.
     应用DNA电泳转移、生物素探针Southern杂交及不同探针重复杂交等3项新技术研究肝炎肝癌的肝内HBV DNA分子状态。
短句来源
     It was identified by Southern blot.
     Southern杂交结果表明重组病毒基因组中含有hPF4基因。
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  transfer blot
Transfer blot analysis of human placental RNA identified transcripts of 2.2 kb and 1.7 kb that appear transiently with increasing levels of expression during gestation.
      
The PCR products were analyzed on 1% agarose gels and confirmed by DNA transfer blot hybridization.
      
ELISA is a better initial step, but positive results must be confirmed with enzyme-linked immunoelectro transfer blot.
      


Schistosoma japonicum eggs were homogenized in phosphate buffered saline (PBS). The precipitate was extracted with 8 M urea. The chemical and immunological properties of the urea soluble antigens (JEU) were compared with those of the aqueous soluble antigens (JSEA) .The total antigenic activity units of JEU were 2.83 times those of JSEA in kinetic dependent enzyme linked immunosorbent assay (K-ELISA). By Bio-Gel A 50 m chromatography, a fraction JEG3 was obtained from JEU. JEG3 was stable and of high antigenic...

Schistosoma japonicum eggs were homogenized in phosphate buffered saline (PBS). The precipitate was extracted with 8 M urea. The chemical and immunological properties of the urea soluble antigens (JEU) were compared with those of the aqueous soluble antigens (JSEA) .The total antigenic activity units of JEU were 2.83 times those of JSEA in kinetic dependent enzyme linked immunosorbent assay (K-ELISA). By Bio-Gel A 50 m chromatography, a fraction JEG3 was obtained from JEU. JEG3 was stable and of high antigenic activity. As a diagnostic antigen in detection of antibody in patient sera by K-ELISA, JEG3 was more sensitive than JSEA. Analysis of JSEA and JEG3 by sodium dodecylsulfate/polyacrylamide gel electrophoresis (SDS-PAGE), enzyme linked immuno-electro-transfer blot (ELIB) of SDS-PAGE, electrofocusing electrophoresis and high performance liquid chromatography (HPLC) showed that JSEA and JEG3 contained different antigenic components. The molecular weight of JSEA components was less than 200,000 daltons. The molecular weight of the main components of JEGS was greater than 400,000 daltons.This study is one of the ptojects of the US-PRC cooperation in Science and Technolgy of Medicine and Public Health. This joint work is carried out by The Institute of Parasitic Diseases, Chinese Academy of Medical Sciences and The Centers for Disease Control, USA

日本血吸虫卵在磷酸盐缓冲盐水(PBS)中制成匀浆。离心后上清液为水溶性抗原(JSEA)。沉淀用8M尿素提取,得尿素溶解性抗原(JEU)。用动力学酶标固相免疫方法(K-ELISA)进行测定,JEU的抗原总活力得量为JSEA的2.83倍。将JEU通过Bio-Gel A 50 m柱分得的JEG_3为稳定的不含尿素的高抗原活力成分。作为诊断用抗原,应用K-ELISA对血吸虫病人血清进行测定,JEG_3较JSEA为敏感。SDS-聚丙烯酰胺凝胶电泳,凝胶区带电泳转移酶标免疫印斑技术,薄膜聚焦电泳和高压液相色谱分析的结果均表明,JSEA和JEG_3含有不同的蛋白质抗原成分,前者各组分的分子量在20万道尔顿以下,后者则主要为大分子蛋白质,分子量在40万道尔顿以上。

Microsome antigens were extracted from homogenate of adult Schistosoma japonicum by differential centrifugation and fractionated by Sepharose CL-2B chromatography. The crude microsome antigen (JAMA-C), the urea soluble fraction (JMC) and the purified microsome antigen (JAMA) were tested with ELISA for their sensitivity and specificity in detecting schistosomal antibody. In kinetic dependent assay (K-ELISA), JAMA showed activity Of 8.8 units with homologous serum. With sera from ascariasis (3), clonorchiasis...

Microsome antigens were extracted from homogenate of adult Schistosoma japonicum by differential centrifugation and fractionated by Sepharose CL-2B chromatography. The crude microsome antigen (JAMA-C), the urea soluble fraction (JMC) and the purified microsome antigen (JAMA) were tested with ELISA for their sensitivity and specificity in detecting schistosomal antibody. In kinetic dependent assay (K-ELISA), JAMA showed activity Of 8.8 units with homologous serum. With sera from ascariasis (3), clonorchiasis (3), paragonimiasis (3) and trichinosis (1), all the results were below 1 unit. In microtitre plate ELISA, all the three microsome antigens showed high sensitivity and specificity. The results indicated that the specific activity increased with the process of purification. In comparison with microsome antigen, the cytosol and urea soluble nucleus antigen showed lower activity. When analysed by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and enzyme linked immunoelectro-transfer blot of SDS-PAGE, JAMA was shown to be a heterogenous material composed of proteins of molecular weight from 14,400 to more than 200,000 daltons and the main antigenic components were beyond 43,000 daltons.

用差速离心和琼脂糖CL-2B柱层析方法自日本血吸虫成虫匀浆中提取和分离微粒体抗原,用ELISA法对微粒体粗抗原(JAMA-C)、尿素溶解部分(JMC)和提纯的微粒体抗原(JAMA)的敏感性和特异性进行了比较。在动力学一ELISA测定中JAMA抗原对同种抗体呈现出高的活力,达到8.8单位。试验蛔虫、肝吸虫、肺吸虫病患者血清各3份,旋毛虫病猴血清1份,结果均低于1单位,未出现交叉反应。在微量滴定板ELISA中亦呈现高敏感性和特异性,证明经过纯化,特异性活力增加。与微粒体抗原相比,胞液及尿素溶解性核抗原的活力很低。SDS-聚丙烯酰胺电泳及SDS-PAGE的电泳转移酶标免疫印斑的结果证明JAMA是由分子量14,400至200,000道尔顿以上的蛋白分子组成的不均一体,主要抗原成分的分子量在43,000道尔顿以上。

The excretory-secretory antigens (ES) of Fasciolopsis buski were prcparcd from In vitro cultivation of the adults. The composition of the ES preperations is quite complex. When analy- sed by gel concentration gradient PAGE and SDS-PAGE, it revealed the presence of about 7 protein components and 9 prolypeptide bands. When analysed by immunoelectrophoresis (IE), im- munodiffusion (ID) and enzyme linked immunoelectro-transfer blot (EL/T) of SDS-PAGE, it showed that there were many common antigen components...

The excretory-secretory antigens (ES) of Fasciolopsis buski were prcparcd from In vitro cultivation of the adults. The composition of the ES preperations is quite complex. When analy- sed by gel concentration gradient PAGE and SDS-PAGE, it revealed the presence of about 7 protein components and 9 prolypeptide bands. When analysed by immunoelectrophoresis (IE), im- munodiffusion (ID) and enzyme linked immunoelectro-transfer blot (EL/T) of SDS-PAGE, it showed that there were many common antigen components between ES and soluble adult antigens, but only a few between ES and soluble egg antigens.

利用体外无菌培养技术,制备了布氏姜片虫的排泄分泌抗原(ES)。经梯度PAGE和SDS-PAGE分别分离出7条蛋白质区带和9条多肽区带,其中包括1条糖蛋白和1条糖脂蛋白,说明布氏姜片虫ES含复杂的蛋白组分。免疫电泳、免疫扩散印迹试验表明,布氏姜片虫ES含有较多与虫体抗原相同的抗原成分,也含有少数与虫卵抗原相同的抗原成分。

 
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