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sterile liquid
相关语句
  无菌液
     According to the features of spore fungus,we studied on its detection technique,screened sterile liquid and found key technique adapted to detect the fungus population.
     根据孢子菌类特征 ,对其检测技术进行探讨。 筛选出检测无菌液 ,找出适于此类菌系检测方法关键技术。
短句来源
  “sterile liquid”译为未确定词的双语例句
     The rate up to standards of the object surfaces, the staff hands, the sterile liquid in use, the air in operation room, the ultraviolet lamp were 73.2 %, 69.3 %, 97.3 %, 68.9 %, 76.4 % respectively.
     其中物体表面、医护人员手、使用中消毒液、手术室空气、无菌物品、紫外线灯的合格率分别为 73.2%、6 9.3%、97.3%、6 8.9%、71.2 %、76 .4 %。
短句来源
  相似匹配句对
     Sterile packaging
     塑料瓶无菌灌装包装
短句来源
     Liquid Crystal
     液晶化学
短句来源
     Job liquid .
     工作液。
短句来源
     Skeletal muscle of hind legs was collected in sterile tube and stored in liquid nitrogen.
     然后麻醉状态下无菌留取下肢骨骼肌组织标本,置消毒的冻存管中,液氮保存待测。
短句来源
     Sterile silicon oil or liquid paraffin was sprayed on the fiber bronchoscope and in catheter.
     纤支镜及导管内均涂以无菌硅油或液体石腊,将无菌气管导管套于纤支镜外。
短句来源
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  sterile liquid
Calli were cut off from the explants and were transferred into a sterile liquid PESI medium in flasks.
      
Heat-treatment (10 min, 80°C) of the non-sterile liquid samples increased transformation only in the dried soil extract, probably by inactivation of DNases.
      
The protein was available as a clear to slightly opalescent, sterile liquid at a concentration of 10 mg/ml.
      
The protein was purified by multiple chromatography steps and available as a clear, sterile liquid.
      
Phenology of 9-d-old N-limited and N-replete Arabidopsis seedlings grown in sterile liquid culture.
      


Cotton seeds collected from plants infected by Verticillium ablo-atrum were used for the purpose of studying seed transmission of the pathogen. Although verticilliate conidiophores had been observed occasionally under low-power microscope on the surface of the seeds plated on PDA in 1955, yet isolations of the organism failed due to the interference of several contaminants. Seeds were delinted in order to diminish the possibility of interference from lint-born contaminants. The materials were plated on different...

Cotton seeds collected from plants infected by Verticillium ablo-atrum were used for the purpose of studying seed transmission of the pathogen. Although verticilliate conidiophores had been observed occasionally under low-power microscope on the surface of the seeds plated on PDA in 1955, yet isolations of the organism failed due to the interference of several contaminants. Seeds were delinted in order to diminish the possibility of interference from lint-born contaminants. The materials were plated on different culture media such as PDA, acidified PDA, cotton meal agar and rice agar. All these plates failed to show verticillium colonies. Among the fungi obtained in these plates, Fusarium, Alternaria and Colletotrichum were the most frequent. Since both Verticillium albo-atrum and other fungi were harbored within the same seed, the difference of growth rates might be considered as an important factor involved in the difficulty of the isolations: Cultures of V. albo-atrum and Fusarium sp. were transferred simutaneously to PDA plates to test their growth rates. The diameter of their colonies were measured after incubation for 12 days under 22±1℃. Those of V. albo-atrum measured 2.8 cm., while the average of the latter was 9,3 cm. Interference of the fast-growers might render a slow-grower such as V. albo-atrum to be masked in the observations and to be depressed in the isolations. A method was developed to avoid the interference. The fundamental procedures of the method are outlined as follows. 1. Plating and observation. (1) Cotton seeds being delinted with commercial surphuric acid in order to avoid the interference from lint-born contaminants. (2) Delinted seeds being washed in a flask covered with wire gauze for 24 hours under running tap water in order to get rid of bacteria. Absorption of sufficient water during washing activates the hibernating mycelium. (3) Seeds being plated on 1.7% water agar instead of nutrient agar in order to diminish the growth of the fast-growers such as Fusaria and Alternaria. The plates being incubated under 22±1℃. for 15 days. (4) The plates being uncovered and observed directly under low-power microscope in order to observe the fungi undisturbed. 2. Isolation and preservation of the Verticillium cultures. (1) Verticilliate conidiophores being located under low-power microscope. (2) The tip of a flamed needle moistened with sterile agar being introduced within the microscopic field in order to fish the spores under focus. (3) Spore suspension being made by washing the needle in a 5 cc sterile water blank. (4) Dilution plates being made by placing 1 cc of the spore suspension in a petri dish and diluting with 10 cc of cotton stem agar. (5) The plates being observed under low-power microscope after incubation for 5—7 days at 22±1℃. (6) Verticillium colonies being locatad and a bit of the margin of a colony being transferred to PDA slant. (7) The slant cultures being flooded with sterile liquid paraffin after incubation for 7—15 days at 22±1℃. The cultures preserved with liquid paraffin were able to keep alived for 2 years. Seeds collected from the infected plants in 1955, 1956 and 1957 were studied. The percentages of seeds carrying Verticillium observed in these years were 39.8, 5.92 and 9.5% respectively. One of the representative single spore colonies obtained was identified as V. albo—atrum. Inoculation and reisolation tests showed positive results. Inspection of cotton seed samples collected from eight co-op farms in Shensi Province in 1958 showing that the percentages of seeds infected by verticillium ranged 3 to 23% with an average of 12.3%.

(1) 在陝西关中所采的黄萎病病株棉籽內部,常带有一些真菌和細菌,虽經用浓硫酸脫絨,这些菌类仍能从棉籽本身的組織內向培养基伸展惺庇玫捅剁R直接检查虽亦能見到輪枝菌从棉籽內露出,但由于其他菌类的生长速度較快,以致輪枝菌常被干扰,无从在洋菜上发展,故用一般方法很难分离和检查到棉籽所带的輪枝菌。(2) 用不含养分的水洋菜作为培养基时,杂菌的生长減弱,轮枝菌才能在基物上发展为菌落。又用流水冲洗法代替表面消毒,使棉籽吸收水分,同时也促使潛伏菌絲继續发展,并可冲去細菌。經試驗用上述两个步驟可以使棉籽所带的輪枝菌在水洋菜上形成菌落。結合低倍鏡直接检查法,可以較为方便地检查种籽带菌率。(3) 經初步拟定了一套检查方法和单孢子菌种分离法。惟应用时尚觉不够簡便,尚有待于进一步提高,特提出以供交流經驗。(4) 在1955—1957年的多次检查中,查得黄萎病病株棉籽带輪枝菌率为39.8%、5.9%及23.7%。在1958年初对大田种子进行检查,查得华阴等四县8个样品中带菌率为3%至23%,平均为12.3%。(5) 根据棉籽解剖培养結果,轮枝菌不仅存在于棉籽外部的短絨內,并且也存在于籽壳及籽仁上。(6) 从棉籽上分离到的轮枝菌特8号...

(1) 在陝西关中所采的黄萎病病株棉籽內部,常带有一些真菌和細菌,虽經用浓硫酸脫絨,这些菌类仍能从棉籽本身的組織內向培养基伸展惺庇玫捅剁R直接检查虽亦能見到輪枝菌从棉籽內露出,但由于其他菌类的生长速度較快,以致輪枝菌常被干扰,无从在洋菜上发展,故用一般方法很难分离和检查到棉籽所带的輪枝菌。(2) 用不含养分的水洋菜作为培养基时,杂菌的生长減弱,轮枝菌才能在基物上发展为菌落。又用流水冲洗法代替表面消毒,使棉籽吸收水分,同时也促使潛伏菌絲继續发展,并可冲去細菌。經試驗用上述两个步驟可以使棉籽所带的輪枝菌在水洋菜上形成菌落。結合低倍鏡直接检查法,可以較为方便地检查种籽带菌率。(3) 經初步拟定了一套检查方法和单孢子菌种分离法。惟应用时尚觉不够簡便,尚有待于进一步提高,特提出以供交流經驗。(4) 在1955—1957年的多次检查中,查得黄萎病病株棉籽带輪枝菌率为39.8%、5.9%及23.7%。在1958年初对大田种子进行检查,查得华阴等四县8个样品中带菌率为3%至23%,平均为12.3%。(5) 根据棉籽解剖培养結果,轮枝菌不仅存在于棉籽外部的短絨內,并且也存在于籽壳及籽仁上。(6) 从棉籽上分离到的轮枝菌特8号經初步鉴定系Verticillium albo-atrum Reinkeet Berth。(7) 特8号菌种經过接种試驗,确定其具有一定的致病力,并从再分离中获得該菌。(8) 棉籽是否能够传播黄萎病,似系一个检查方法問題。还希各方面进行测定,通过多次的检查和接种試驗始能充分确定。(9) 在分离的菌种中,除特8号菌种具有較深的菌落色泽外,还有一些菌种的顏色較淡,未須鑑定。棉籽所带的轮枝菌类型不一,还有进一步研究的必要。此外,棉籽內的輪枝菌和其他一些菌类究竟如何进入籽內,也有研究的价值。

Fast-growing R. japonicum QB113 was isolated from nodules of wild soybean(Glycine soja) growing in northeast china. In yeast extract mannitol liquid medium its mean generation time was 3.22 hours and it produced acid. Its physiological-biochemical characteristics were similar to other fast-growing R. japonicum reported. For the first time it was reported that R. japonicum QB113 showed both good infectiveness and effectiveness with common commercial cultivars of soybean in the greenhouse and field experiments....

Fast-growing R. japonicum QB113 was isolated from nodules of wild soybean(Glycine soja) growing in northeast china. In yeast extract mannitol liquid medium its mean generation time was 3.22 hours and it produced acid. Its physiological-biochemical characteristics were similar to other fast-growing R. japonicum reported. For the first time it was reported that R. japonicum QB113 showed both good infectiveness and effectiveness with common commercial cultivars of soybean in the greenhouse and field experiments. In sterile liquid culture experiment, seed yield of Tiefeng 18 inoculated with QB113 increased 121.7% than that of control, and 55.5% than that of plants inoculated with fast-growing R. japonicum USDA193, and 9.1% than that of plant inoculated with slow-growing R. japonicum 61A76. The same treatments were conducted on Heinong 26, Hefeng 25, 82-7799-2 soybean varieties in field experimental plot, and 20 hectare in the South(planosol area) and North(black soil area)of Wan Da mountain, Heilongjiang Province. The seed yield of plant inoculated with QB113 increased 8.6%, 10.0% and 15.7% respectively than that of uninocu-laed. The results of our experiments showed that the fast-growing R. japonicum QB113 can form effective symbiosis with the soybean culti-var, and proposed to a definite valuable prospects in increasing the yield of soybean production.

从东北地区分离的快生型大豆根瘤菌QB113(fast-growing R.japonicum QB113)在酵母——甘露醇液体培养基上生长时,世代时间为3.22小时,代谢过程产酸,生理生化特性与国内外报导的快生型大豆根瘤菌相类似。我们首次报导了快生型大豆根瘤菌对栽培大豆表现的良好共生固氮能力和增产效果。在实验室的无菌水培实验中,铁丰18大豆接种QB113,种子产量比不接菌的对照提高121.7%,比接种快生型大豆根瘤菌USDA193的提高55.5%,比接种慢生型大豆根瘤菌61A76的提高9.1%,在黑龙江省莞达山南麓(白浆土地区)、北麓(黑土地区)的田间小区试验和大面积(20公顷)试验中,对黑农26、合丰25、82—7799—2大豆进行同样的接菌处理,接种QB113的种子产量比对照分别提高8.6%、10.0%和15.7%。结果说明快生型大豆根瘤菌QB113是可以与栽培大豆品种形成有效共生的。并且,在生产应用中具有一定的价值和可观的前景。

The alumina tubular membrane prepared by the meltable moulding centrifugal technique has gradient pore size distribution in cross section with the narrow pore size distribution of top layers(controlled layers). With the most pore size of 005μm,the largest pore size of 0.1μm alumina tubular membrane, very bright and entire sterile water is obtained. Using the tubular membrane of the most pore size of 0.1μm and the largest pore size of 0.2μm, the bacterium in the feed can be removed with stable microfiltration...

The alumina tubular membrane prepared by the meltable moulding centrifugal technique has gradient pore size distribution in cross section with the narrow pore size distribution of top layers(controlled layers). With the most pore size of 005μm,the largest pore size of 0.1μm alumina tubular membrane, very bright and entire sterile water is obtained. Using the tubular membrane of the most pore size of 0.1μm and the largest pore size of 0.2μm, the bacterium in the feed can be removed with stable microfiltration flux of 0.45m3/(m2·h). Nearsterilizing filtration can be achieved using membranes of the most pore size of 0.2μm and the largest pore size of 0.3μm, escherichia coli, mold and yeast in water can be removed entirely, more than 95% of total bacteria contained in the water can be removed. The crossflow microfiltration flux starts at a high level and drops rapidly, approaching a steadystate value of 1.59m3/(m2·h) for water. This kind of asymmetric tubular membrane minimizes deeppore fouling and plugging efficiently, easy cleaning, with 100% recovery of trapping efficiency and more than 90% recovery of flux efficiency. This kind of alumina tubular membrane can be used in the food and beverage industries and in water filtration to produced a clear sterile liquid and water by removing the contaminants such as microparticles of 0.1—10μm is diameter or bacterium.

采用熔模离心法制备的不对称氧化铝微滤膜管的孔径沿径向呈梯度分布,控制层孔径均匀.最可几孔径为005μm、最大孔径为01μm的管能滤除原液中全部的细菌,获得完全无菌的水,但过滤速率太慢.最可几孔径为01μm、最大孔径为02μm的管能基本滤除原液中的全部细菌,获得无菌的水,过滤速率为045m3/(m2·h).最可几孔径为02μm、最大孔径03μm的氧化铝膜管能完全滤除水中的大肠杆菌、霉菌和酵母,细菌总滤除率大于95%,水的错流微滤通量可稳定在159m3/(m2·h).这种孔径梯度分布的不对称氧化铝膜管能有效防止膜深层孔的堵塞,延长微滤膜管的寿命,细菌滤除性能、渗透性能恢复率良好,分别为100%和大于90%.可用作食品和饮料工业(水果汁)的液体过滤、水的微滤,除去01—10μm的微生物和微粒子,生产无菌液体、澄清液体和无菌水.

 
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