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insecticidal protein expressed
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  杀虫蛋白
     Studies on the Enzyme-linked Immunosorbent Assay for Detection of Bacillus thuringiensis Insecticidal Protein Expressed in Transgenic Plants Ⅱ.Preparation of Polyclonal Antibody to Bt Insecticidal Protein
     转Bt基因植物中杀虫蛋白的酶联免疫检测技术研究Ⅱ.Bt杀虫晶体蛋白抗体的制备
短句来源
     Studies on the Enzyme-linked Immunosorbent Assay for Detection of Bacillus thuringiensis Insecticidal Protein Expressed in Transgenic Plants Ⅰ.Preparation of Bt Strain HD-1 Insecticidal Protein (Antigen)
     转Bt基因植物中杀虫蛋白的酶联免疫检测技术研究Ⅰ.Bt菌HD-1的杀虫晶体蛋白抗原制备
短句来源
     Recombinant Phage-aided Detection of Bt Insecticidal Protein Expressed in Transgenic Plants
     转基因植物中Bt杀虫蛋白的重组噬菌体辅助检测
短句来源
     The Study of Bacillus Thuringiensis Insecticidal Protein Expressed in the Tissue of Xinjiang Transgenic Insect Resistant Cotton
     新疆转基因抗虫棉组织中Bt杀虫蛋白表达的研究
短句来源
     Studies on the Enzyme-linked Immunosorbent Assay for Detection of Bacillus thuringiensis Insecticidal Protein Expressed in Transgenic Plants Ⅲ.Detection of Bt Insecticidal Protein by Indirect Enzyme-linked Immunosorbent Assay
     转Bt抗虫基因植物中杀虫蛋白的酶联免疫检测技术研究 Ⅲ.酶联免疫(ELISA)间接法检测Bt杀虫蛋白研究
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  “insecticidal protein expressed”译为未确定词的双语例句
     Purification of antigen, preparation of specific antibody and enzyme-labelled antibody and ELISA method were studied for detection of Bt insecticidal protein expressed in Bt transgenic plants.
     本研究以目前抗虫转基因育种中研究最多的Bt CryIA(C)基因的表达产物为检测目标,在提取出高纯度抗原并制备出高特异性抗体的基础上,系统研究了两种载体上三种酶联免疫吸附测定方法的检测灵敏度,初步建立起快速、准确的酶联免疫检测技术。
短句来源
     The concentrations of Cry1Ab insecticidal protein expressed in pollens of transgenic Bt rice KMD1 and KMD2 from a commercial Chinese japonica rice variety Xiushui 11(XS11),contain a syn- thetic cry1Ab gene from the bacterium Bacillus thuringiensis (Bt) Berliner,were detected by enzyme im- munosorbent assay (ELISA) with the Quantiplate~(TM) kit for Bt Cry1Ab protein.
     用 ELISA 法测定了转 Bt 基因水稻克螟稻1号(KMD1)和克螟稻2号(KMD2)花粉中Cry1Ab 含量。 将转 Bt 基因水稻和其亲本对照秀水11(xs11)花粉加入到草蛉人工干粉饲料中,评价了对中华草蛉 Chrysopela sinica Tjeder 成虫产卵和寿命等参数的影响。
短句来源
     Quantification Using ELISA of Bacillus thuringiensis Insecticidal Protein Expressed in the Tissue of Transgenic Insect Resistant Cotton
     转基因抗虫棉组织中Bt毒蛋白表达量的ELISA测定
短句来源
     On the Enzyme linked Immunosorbent Assay of Bacillus thuringiensis Insecticidal Protein Expressed in Transgenic Cotton
     转Bt基因棉Bt毒蛋白的ELISA检测方法的研究(英文)
短句来源
     Studies on the Enzyme-linked Immunosorbent Assay for Detection of Bacillus Thuringiensis Insecticidal Protein Expressed in Transgenic Plants
     转Bt抗虫基因植物的酶联免疫检测技术研究
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  相似匹配句对
     protein.
     protein。
短句来源
     Studies on Structure of Pseudomonas pseudoalcaligenes Insecticidal Protein.
     类产碱假单胞菌杀虫蛋白的激光拉曼光谱研究
短句来源
     UV-Protection of Bt Melanin to Insecticidal Protein
     Bt黑色素对杀虫晶体蛋白的紫外保护
     mg protein.
     mg。
短句来源
     Study on ELISA for Detection of Bacillus Thuringiensis Insecticidal Protein Expressed in Transgenic Plants
     转Bt抗虫基因植物的酶联免疫检测技术研究
短句来源
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Based on the reaction among antibody, antigen and enzyme antibody, an indirect enzyme linked immunosorbent assay (ELISA) method was developed to detect and quantitate the Bacillus thuringiensis (Bt) insecticidal protein expressed in the tissue of Bt transgenic insect resistant cotton. Young leaves from transgenic insect resistant cotton of strain 310 at initial squaring stage were analyzed by ELISA, and showed that the content...

Based on the reaction among antibody, antigen and enzyme antibody, an indirect enzyme linked immunosorbent assay (ELISA) method was developed to detect and quantitate the Bacillus thuringiensis (Bt) insecticidal protein expressed in the tissue of Bt transgenic insect resistant cotton. Young leaves from transgenic insect resistant cotton of strain 310 at initial squaring stage were analyzed by ELISA, and showed that the content of Bt insecticidal protein in the leaves averaged up to 0 126% of the total soluble protein. This explains the insect resistance of the cotton strain 310 which has been developed by introduction of Bt insecticidal protein gene through the pollen tube pathway into a common cotton cultivar. The minimal detectable value of purified Bt insecticidal protein was 20 ng/ml by this method.

利用抗体-抗原-酶标抗体反应,建立了间接酶联免疫吸附测定法(ELISA),以检测转基因抗虫棉品系310幼叶Bt毒蛋白含量。初步测定结果:转基因抗虫棉幼叶中Bt毒蛋白含量为总可溶性蛋白质的(0.126±0.008)%。提纯的伴胞晶体样品经ELISA法检测,其Bt毒蛋白最低可检值为20ng/ml。这一检测技术既可对转基因抗虫棉组织中Bt毒蛋白表达量进行定性、定量测定,也为阐明转基因抗虫棉的抗虫特性提供了理论依据。

An antibody sandwich enzyme linked immunosorbent assay (ELISA) was developed to detect and quantitate the Bacillus thuringiensis (Bt) insecticidal protein expressed in transgenic insect resistant cotton using a purified Bt toxin (67kDa) as standard protein and antigen. The minimal detectable concentration of purified Bt toxin was 1μg·L -1 and the linear assay range of the method was 1μg·L -1 to 15μg·L -1 .Both the errors of intra and interplate(s) were <5%,and the mean recovery...

An antibody sandwich enzyme linked immunosorbent assay (ELISA) was developed to detect and quantitate the Bacillus thuringiensis (Bt) insecticidal protein expressed in transgenic insect resistant cotton using a purified Bt toxin (67kDa) as standard protein and antigen. The minimal detectable concentration of purified Bt toxin was 1μg·L -1 and the linear assay range of the method was 1μg·L -1 to 15μg·L -1 .Both the errors of intra and interplate(s) were <5%,and the mean recovery rate was 94.94%.It was indicated by quality controal experiments that this ELISA method was stable and reliable ,and it can be used in the detectiomn of Bt toxin content expressed in transgenic cotto.

利用纯化的Bt毒素蛋白(67kDa)作为标准蛋白和免疫抗原,建立了检测转基因抗虫棉植株内Bt毒蛋白含量的抗体夹心ELISA方法。检测极限1μg·L- 1,线性范围1- 15μg·L- 1,板内误差C.V.< 5% ,板间误差C.V.< 5% ,平均样品回收率94.94% 。经稀释度试验等质量控制试验,表明所建立的ELISA方法是可靠的,可用于转基因棉花植株中Bt毒素蛋白含量的测定。

In the paper, the current progress in the resistance of transgenic cotton to cotton boll worm(Helicoverpa armigera Hubner) and its variation with the differences of cotton structure and the development of cotton growth stages was reviewed. The toxicology to cotton boll worm of the insecticidal proteins expressing in Bt trangsgenic cotton and its physiological effects , the determination methods of the insecticidal proteins expressing in transgenic cottons, the dynamic expression of the pest resistance...

In the paper, the current progress in the resistance of transgenic cotton to cotton boll worm(Helicoverpa armigera Hubner) and its variation with the differences of cotton structure and the development of cotton growth stages was reviewed. The toxicology to cotton boll worm of the insecticidal proteins expressing in Bt trangsgenic cotton and its physiological effects , the determination methods of the insecticidal proteins expressing in transgenic cottons, the dynamic expression of the pest resistance and the influence of fertilizer, temperature and soil moisture were discussed. Some new points to be probed were proposed.

综述了转Bt基因棉对棉铃虫存活、生长、繁殖及为害行为的影响 ,及其抗虫性的时空动态特征 ;探讨了转Bt基因棉抗棉铃虫的毒理及其中毒生理 ,以及转Bt基因棉的抗性表达及其影响因素 ,提出了一些尚待进一步深入研究的问题

 
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