助手标题  
全文文献 工具书 数字 学术定义 翻译助手 学术趋势 更多
查询帮助
意见反馈
   double-gradient 的翻译结果: 查询用时:0.189秒
图标索引 在分类学科中查询
所有学科
更多类别查询

图标索引 历史查询
 

double-gradient
相关语句
  双梯度
     DNA Fragments Separating by Double-gradient Super-thin PAGE and Recovering from Silver-stained Gels
     双梯度超薄胶PAGE分离DNA及其银染与回收
短句来源
     Research of Double-gradient Chloride Dioxide Reactor
     双梯度二氧化氯反应器的研究
短句来源
     Fractionation of PAM was prepared by means of a double-gradient elution and the of each fraction were determined by the viscosity method.
     采用了双梯度淋洗法对高分子量的聚丙烯酰胺(PAM)进行柱上制备分级,利用粘度法测定各级分的分子量,得到分子量的积分和微分分布曲线,由曲线可知其和未分级样品的相比较接近,表明分级方法可靠。
短句来源
     Methods This study included 36 CHD patients, 28 non-CHD patients and 8 normal control subjects, from whom serum βLps (prestained with Sudan Black B) were isolated by means of the first double-gradient polyacrylamide gel electrophoresis (f-DGPAGE). The secondary DGPAGE was performed for isolating βLps into 4 subfractions (βLp1-4) in order to complete the quantitative analysis of βLp electrophoretogram.
     方法选择CHD患者36例、非CHD患者28例、正常对照者8例,将一阶双梯度聚丙烯酰胺凝胶电泳法分离出的苏丹黑B预染的血清βLp,经二阶双梯度聚丙烯酰胺凝胶电泳法再分离出亚组分βLp1~4,完成βLp谱的量化和分析。
短句来源
  相似匹配句对
     Research of Double-gradient Chloride Dioxide Reactor
     双梯度二氧化氯反应器的研究
短句来源
     Double-Target Identification on Improved Natural Gradient Algorithm
     基于改进自然梯度算法的双目标辨识研究
短句来源
     The Double Blanket
     双人毛毯
短句来源
     Double Faces
     双重表情
短句来源
     gradient elution;
     梯度洗脱;
短句来源
查询“double-gradient”译词为用户自定义的双语例句

    我想查看译文中含有:的双语例句
例句
为了更好的帮助您理解掌握查询词或其译词在地道英语中的实际用法,我们为您准备了出自英文原文的大量英语例句,供您参考。
  double-gradient
Double-gradient denaturing gradient gel electrophoresis (DG-DGGE) was used to generate banding patterns from the amplification products of 16S rRNA genes in 1-cm sediment depth fractions.
      
Analysis of Bacterial Communities in Seagrass Bed Sediments by Double-Gradient Denaturing Gradient Gel Electrophoresis of PCR-Am
      
Conditions were developed to screen the ABCR gene by double-gradient denaturing-gradient gel electrophoresis.
      
Mutational scanning of the ABCR gene with double-gradient denaturing-gradient gel electrophoresis (DG-DGGE) in Italian Stargardt
      
The basis of the double-gradient RP HPLC, employing simultaneous gradients of organic modifier content and mobile phase pH, was also elaborated.
      


Fractionation of PAM was prepared by means of a double-gradient elution and the of each fraction were determined by the viscosity method. The cumulative and differential distributions were established, from which some data was obtained as follows: d=1.75, and the dilute solution properties and rheological properties were also studied.

采用了双梯度淋洗法对高分子量的聚丙烯酰胺(PAM)进行柱上制备分级,利用粘度法测定各级分的分子量,得到分子量的积分和微分分布曲线,由曲线可知其和未分级样品的相比较接近,表明分级方法可靠。同时发现PAM稀溶液的[η]与放置时间、超声波震荡时间均有关系。采用Brabender流变仪初步研究了PAM稀溶液的流变特性。

Purified endothelial cell growth factor(ECGF)was prepared from emulsified fresh bovine brain by fractionation (ammonium sultate precipitation method),chromatography on Amberlite IRC-50 and elution with double gradients containing 0~1 mol/L and 1~2mol/L NaCl. The eluates containing ECGF(10 μl each)were added to HUVEC culture to test the stimulating effect on cell proliferation by using colorimetric MTT assay.It was found that ECGF stimulated HUVEC proliferation in the presence of 10% NBS or FCS,HUVEC proliteration...

Purified endothelial cell growth factor(ECGF)was prepared from emulsified fresh bovine brain by fractionation (ammonium sultate precipitation method),chromatography on Amberlite IRC-50 and elution with double gradients containing 0~1 mol/L and 1~2mol/L NaCl. The eluates containing ECGF(10 μl each)were added to HUVEC culture to test the stimulating effect on cell proliferation by using colorimetric MTT assay.It was found that ECGF stimulated HUVEC proliferation in the presence of 10% NBS or FCS,HUVEC proliteration was a function of the ECGF concentration in culture medium, the estimated AC50 was 10 mg/L and the maximal stimulating effect of ECGF was found at the concentration of 25mg/L,where the proliferation was increased to 550%(5.5 times higher than the control).

报道从牛脑制备内皮细胞生长因子(ECGF)的方法。鲜牛脑经匀浆、硫酸铵盐析、透析后,上AmberliteIRC-50离子交换柱,并用氯化钠梯度洗脱。利用ECGF对人脐静脉内皮细胞(HUVEC)的增殖刺激效应测定其活性。当所得ECGF制品在含10%新生牛血清或胎牛血清的培养液中含量为25mg/L时,其最大刺激活性比对照组增加550%。

DDRT PCR amplification products were separated in double gradient super thin PAGE. The results showed that single base pair(bp)difference between two DNAs was clearly detected in 30 cm long gels, and over 100 bands were completely separated by the double gradient PAGE. On the other hand the objective bands could be directly recovered from silver stained PAGE gels. The method issimply, economical,and makes it possible availably to analyes gene xpression.

双浓度梯度超薄胶 PAGE分离 DDRT-PCR扩增产物表明 ,在 3 0 cm长的凝胶板上能清晰分辨长度仅差 1 bp的 DNA,清楚的显示出 1 0 0多条分离谱带 ,并可直接从银染 PAGE胶中回收 DNA谱带。简便节省 ,是基因表达分析的有效方法

 
<< 更多相关文摘    
图标索引 相关查询

 


 
CNKI小工具
在英文学术搜索中查有关double-gradient的内容
在知识搜索中查有关double-gradient的内容
在数字搜索中查有关double-gradient的内容
在概念知识元中查有关double-gradient的内容
在学术趋势中查有关double-gradient的内容
 
 

CNKI主页设CNKI翻译助手为主页 | 收藏CNKI翻译助手 | 广告服务 | 英文学术搜索
版权图标  2008 CNKI-中国知网
京ICP证040431号 互联网出版许可证 新出网证(京)字008号
北京市公安局海淀分局 备案号:110 1081725
版权图标 2008中国知网(cnki) 中国学术期刊(光盘版)电子杂志社