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  vip受体
    Experimental research for tumor VIP receptor imaging
    肿瘤VIP受体显像的实验研究
短句来源
    Conclusion VIP receptor mediated endocytosis pathway could highly increase the access of 99 Tc m ASON into HT29 cell and the 99 Tc m ASON entering into cells could effectively inhibit cell proliferation and C myc cancer protein expression.
    结论 VIP受体介导途径能显著增加99Tcm ASON的转染效率 ,明显抑制HT2 9细胞的生长和C myc癌蛋白质的表达 ,增强反义抑制作用效果。
短句来源
    To study the possibility of radioactive labelled vasoactive intestinal peptiede (VIP) for tumor VIP receptor imaging.
    探讨了放射性碘标记血管活性肠肽(VIP)实现肿瘤VIP受体显像的可能性。
短句来源
    The present study demonstrated that the radioactive labelled VIP is a promosing agent for tumor VIP receptor scintigraphy.
    本研究表明放射性核素标记VIP可望成为一有效的肿瘤VIP受体显像剂。
短句来源
    The present study demonstrated that the binding sites of VIP on human gastrointestinal adenocarcinoma were more abundant than their adjacent tissue membrane, which provide valuable experimental evidence for VIP receptor imaging.
    研究结果表明人胃肠腺癌细胞膜与其癌旁组织细胞膜相比, VIP受体亲和力无明显变化,最大结合容量明显增加,为VIP受体显像奠定了直接的实验依据。
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  “vip receptor”译为未确定词的双语例句
    Study of binding of ~(125)I-VIP with VIP receptor in H22 hepatocarcinoma cells in mice
    ~(125)I-VIP与小鼠肝癌H22细胞受体结合特性研究
短句来源
    RESULTS: VIP of 10-9,10-8, 10-7,10-6 and 10-5 mol/L significantly promoted the growth of AsPC-1 cells and maximum activated effect was exerted by 10-8. The proliferation of AsPC-1 cells could be inhibited by (D-P-cl-Phe6-Len17)VIP, which was the antagonist of VIP receptor.
    结果:VIP(10~(-9)~10~(-5)moL/L)对胰腺癌细胞株AsPC-1有明显促生长作用,细胞增生最强的浓度是10~(-8)moL/L,且这种作用能被受体的拮抗剂(D-P-cl-Phe6-ken17)VIP所阻断; 胰腺癌细胞株AsPC-1上有VPAC1 mRNA的表达;
短句来源
    Results The uptake rate of HT29 cell exposed to 99 Tc m VIP ASON complex was highly increased by VIP receptor mediated endocytosis, the highest uptake rate was 27.39% at 2 h and significantly higher than that exposed to 99 Tc m ASON ( P <0.05).
    结果 摄取研究显示 ,各时间点HT2 9细胞对99Tcm VIP ASON的摄取均高于99Tcm ASON(P <0 .0 5 ) ,摄取率在 12 0min达最高 ,为 2 7.39%。
短句来源
    Antitumor effects of radioiodinatedantisense oligonuclide mediated VIP receptor
    ~(131)I-VIP-ASON在荷结肠腺癌裸鼠体内的抑瘤作用
短句来源
    99 Tc m ASON was complexed with VIP under certain condition. The HT29 cells were incubated with the complex and the uptake rate of 99 Tc m VIP ASON was assayed. The effect of 99 Tc m VIP ASON on cell growth and C myc cancer protein expression was studied by VIP receptor mediated endocytosis.
    方法 用99Tcm 标记长度为 15个碱基互补于C mycmRNA的反义寡核苷酸 (ASON) ,在一定条件下与VIP形成99Tcm VIP ASON复合物 ,测定HT2 9细胞对99Tcm VIP ASON的摄取率以及99Tcm VIP ASON对HT2 9细胞生长和C myc癌蛋白质表达的影响。
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  vip receptor
Concentration-response curves for nitroglycerin, vasoactive intestinal polypeptide (VIP), and sodium nitroprusside (SNP) were shifted to the right in the presence of (p-chloro-d-Phe6, Leu17)-VIP (VIPa), a VIP receptor antagonist.
      
The results indicate that VIP receptor antagonists increase the ability of chemotherapeutic drugs to kill breast cancer cells.
      
VIP receptor antagonists and chemotherapeutic drugs inhibit the growth of breast cancer cells
      
Displacement of Fluo-VIPTM by secretin indicated the probable presence of VIP receptors of type VPAC1 (VIP receptor subtype1) in the rat breast.
      
These results do not support the hypothesis that HIV through peptide T may interact with the VIP receptor-effector system present in immunocompetent cells.
      
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To study the possibility of radioactive labelled vasoactive intestinal peptiede (VIP) for tumor VIP receptor imaging. 125I-VIP was prepared by chloramine-T method, and purified by sephadex G-50 column chromatography. The bioactivity and stability of 125I-VIP were measured by silica 60 F254 TLC and competition test to SGC7901 cell in vitro. The biodistribution of 125I-VIP was studied in the nude mice bearing tumor. The results showed that labelled rate of 125I was 73.8%, the specific...

To study the possibility of radioactive labelled vasoactive intestinal peptiede (VIP) for tumor VIP receptor imaging. 125I-VIP was prepared by chloramine-T method, and purified by sephadex G-50 column chromatography. The bioactivity and stability of 125I-VIP were measured by silica 60 F254 TLC and competition test to SGC7901 cell in vitro. The biodistribution of 125I-VIP was studied in the nude mice bearing tumor. The results showed that labelled rate of 125I was 73.8%, the specific activity was 18.2PBq/mol, the radiochemical purity(RCP) was over 98% and remained 96.3% after 48 days stored at m80oC. The specific binding of 125I-VIP to the SGC7901 cell was inhibited by VIP in dose dependence in the competition experiment. The radioactivity of tumor was higher than that of muscles in all phases (P<0.05-0.01),the peak activity of tumor occured at 30min (3.58 ± 0.48ID%/g) and the peak ratio of T/N occured at 60min after the injection. The activity of lungs was obviously higher than that of blood, the intestine was always in low level. Most of the activity in the body was mainly eliminated from kidney. The present study demonstrated that the radioactive labelled VIP is a promosing agent for tumor VIP receptor scintigraphy.

探讨了放射性碘标记血管活性肠肽(VIP)实现肿瘤VIP受体显像的可能性。应用氯胺-T法标记、SephadexG-50柱层析分离纯化制备125I-VIP,硅胶TLC与体外竞争结合实验检测标记VIP的稳定性与生物活性;研究125I-VIP在荷SGC7901裸鼠体内分布。结果显示:125I标记率为73.8%;标记VIP的比活度为18.2PBq/mol;放化纯为98%,-80℃贮存48天后为96.3%;VIP以剂量依赖方式抑制125I-VIP与SGC7901细胞结合;各时相肿瘤放射性均高于肌肉(P<0.05—0.01),高峰摄取时间为30min(3.58±0.48%ID/g),T/N峰比值(3.05)出现于60min;肺放射性高于血液(P<0.05),肠道放射性始终处于低水平;体内放射性主要经肾脏排泄。本研究表明放射性核素标记VIP可望成为一有效的肿瘤VIP受体显像剂。

Purpose To investigate the preparation of 125 I vasoactive intestinal peptide (VIP) and its characters of binding to SGC7901 cell receptors Methods VIP was labeled with Na 125 I using chloramine T method, then isolated by sephadex G 50 column chromatography and examined by silica 60F 254 thin layer chromatography (TLC) The tests of time temperature binding, reversible binding, saturable binding and competition binding between SGC7901 cells and 125 I VIP...

Purpose To investigate the preparation of 125 I vasoactive intestinal peptide (VIP) and its characters of binding to SGC7901 cell receptors Methods VIP was labeled with Na 125 I using chloramine T method, then isolated by sephadex G 50 column chromatography and examined by silica 60F 254 thin layer chromatography (TLC) The tests of time temperature binding, reversible binding, saturable binding and competition binding between SGC7901 cells and 125 I VIP were carried out Results The labelling rate of 125 I was 73 8%, the specific activity of 125 I VIP was 18 2TBq/mmol, the radiochemical purity was over 98 2% and remained 96 3% after a 48 days' storage at -80℃ The results demonstrated that the binding of 125 I VIP to SGC7901 cells possessed the reversibility, specificity, saturability, and was time temperature dependent It was confirmed that VIP promoted the production of cAMP and the synthesis of DNA in SGC7901 cells dose dependently Scatchard plotting suggested that SGC7901 cells contained two types of VIP receptors, high affinity receptor and low affinity receptor The concentrations of Kd and B max were 0 230nmol/L and 0 120pmol/10 6 cells for high affinity receptor, 2 788nmol/L and 5 607pmol/10 6 cells for low affinity receptor Conclusions The present study indicates that the labeled 125 I VIP can be used for receptor analysis and the iodination of VIP does not change its binding to VIP receptor We are the first who have confirmed that the SGC7901 cell line expresses VIP receptors of high density and affinity

目的探讨高比活度125I血管活性肠肽(VIP)的制备及其与人SGC7901胃腺癌细胞受体体外结合特性。方法125IVIP采用氯胺T法标记,SephadexG50柱层析分离纯化,硅胶60F254薄板层析检测。125IVIP与SGC7901细胞进行体外时间温度结合实验、可逆结合实验、饱和结合实验和竞争结合实验,通过125IUdR参入实验与cAMP生成实验评价VIP对SGC7901细胞的生物效应。结果125I标记率为738%,125IVIP比活度为182TBq/mmol,放射化学纯度(RCP)为982%,-80℃贮存48天后RCP仍有963%;125IVIP与SGC7901细胞结合具有温度与时间依赖性、可饱和性、可逆性及特异性,Scatchard作图提示SGC7901细胞含有高低亲和力两种VIP受体,Kd分别为0230与2788nmol/L,Bmax为0120与5607pmol/106细胞;VIP以剂量依赖方式促进SGC7901细胞cAMP产生和DNA的合成。结论制备的125IVIP符合作为VIP受体特异性配体的标准,碘标记VIP并不改变与其受体的结合活性;首次证实SGC790?

To investigate the characteristics of ~(125)I-VIP binding to human gastrointestinal adenocarcinoma and their adjacent tissue. VIP was labeled with Na~(125)I using chloramine -T method, separated by Sephadex G-50 column chromatography and examined by silica 60F_(254) thin layer chromatography. The effects of time and temperature on the specific binding were examined, saturable binding and competitive binding between ~(125)I-VIP and VIP receptors in membranes from gastrointestinal adenocarcinoma...

To investigate the characteristics of ~(125)I-VIP binding to human gastrointestinal adenocarcinoma and their adjacent tissue. VIP was labeled with Na~(125)I using chloramine -T method, separated by Sephadex G-50 column chromatography and examined by silica 60F_(254) thin layer chromatography. The effects of time and temperature on the specific binding were examined, saturable binding and competitive binding between ~(125)I-VIP and VIP receptors in membranes from gastrointestinal adenocarcinoma and their adjacent tissue were carried out. The results showed that labeling rate was 70%, the specific activity of ~(125)I-VIP was 18TBq/mmol, the radiochemical purity was better than 98%. A single specific binding sites of high affinity were present in gastric adenocarcinoma and its adjacent tissue as well as in colon adenocarcinoma and its adjacent tissue. The K_d and B_(max) values were 1.19nmol/L and 339fmol/mg protein, 1.26nmol/L and 156fmol/mg protein, 0.97nmol/L and 304fmol/mg protein, 1.53nmol/L and 151fmoll/mg protein respectively. The present study demonstrated that the binding sites of VIP on human gastrointestinal adenocarcinoma were more abundant than their adjacent tissue membrane, which provide valuable experimental evidence for VIP receptor imaging.

探讨~(125)I-血管活性肠肽(VIP)与人胃腺癌及癌旁组织细胞膜、结肠腺癌及癌旁组织细胞膜的体外受体结合特性。~(125)I-VIP采用CH-T法制备, Sephadex G-50柱层析纯化,硅胶60F_(254)薄板层析(TLC)检测。~(125)-I-VIP与人胃肠腺癌及癌旁组织细胞膜进行体外时间-温度结合实验、饱和结合实验和竞争结合实验。结果显示:碘标记率70%,~(125)I-VIP比活度18TBq/mmol,放射化学纯度98%;~(125)I-VIP与人胃腺癌及癌旁组织细胞膜、结肠腺癌及癌旁组织细胞膜的结合具有温度与时间依赖性、可饱和性及特异性;Scatchard作图均为一直线,提示上述组织细胞膜含有单一位点VIP受体;胃腺癌细胞膜及癌周组织细胞膜、结肠腺癌细胞膜及癌周组织细胞膜上VIP受体的K_d值分别为1.91、1.26、0.97、1.53nmol/L;B_(max)分别为339、156、304、 151fmol/mg。研究结果表明人胃肠腺癌细胞膜与其癌旁组织细胞膜相比, VIP受体亲和力无明显变化,最大结合容量明显增加,为VIP受体显像奠定了直接的实验依据。

 
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