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express and purify
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  表达和纯化
     Objective To construct prokaryotic expression vector pET15b-PEP-1-SOD1 and express and purify PEP-1-SOD1 fusion protein.
     目的构建原核表达载体pET15bPEP1SOD1,并进行PEP1SOD1融合蛋白表达和纯化
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     [Objective] Clone, express and purify wild type and 3 deletion mutants of human PDIP38 glutathion S transferase (GST)fusion protein.
     [目的]克隆表达和纯化野生型及3种缺失突变型人PDIP38谷胱甘肽S转移酶(glutathion S trans-ferase,GST)融合蛋白。
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     Objective: To express and purify human interleukin-10(IL-10) in Escherichia coli(E.coli).
     目的:在大肠杆菌(E.coli)中表达和纯化人白细胞介素-10(IL-10)。
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     Objective To construct the pGEX-4T-2-CblN/Grb2, express and purify the corresponding chimeric protein; To investigate whether the chimeric CblN/Grb2 possesses ubiquitin ligase activity.
     目的构建CblN/Grb2嵌合分子,表达和纯化GST-CblN/Grb2融合蛋白,并考察嵌合分子CblN/Grb2在体外是否具有泛素连接酶活性。
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     Objective: To express and purify recombinant protein nm23-M2 by gene engineering method,then produce anti-NM23-M2 serum with high titer and specificity.
     目的:通过基因工程技术表达和纯化重组nm23-M2蛋白,进而制备高效价、高特异性的抗血清。
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  表达及纯化
     Objective To construct the prokaryotic expression vector of HSP65-T cell epitopes of MOMP of C. trachomatis (Ct) fusion protein (called H-ctml), express and purify H-ctml.
     目的:构建热休克蛋白65(HSP65)与沙眼衣原体(C.trachomatis,Ct)主要外膜蛋白(MOMP) T细胞表位(简称ctml)融合蛋白(简称H-ctml)的原核表达载体,并将其表达及纯化
短句来源
     Objective:To express and purify human interferon ω(IFN_ω) in yeast Pichia pastoris to evaluate its functions.
     目的 :在巴斯德毕赤酵母中表达及纯化人ω干扰素 (interferonω ,IFN_ω)以对其功能进行深入研究。
短句来源
     Objective To construct prokaryotic expression plasmid of His-tagged mouse spermatogenesis related gene SRG-S, further to express and purify the recombinant protein.
     目的构建His标记的小鼠精子发生相关基因SRG-S的原核表达载体并实现其表达及纯化
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     Objective :To construct,express and purify the mutant and the truncated hHBRK1 proteins.
     目的:构建微丝相关蛋白hHBRK1截短体和突变体原核表达载体,并表达及纯化融合蛋白。
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     Objective:To Express and purify the EgA31 C terminal peptide for Echinococcusis diagnosis and vaccine.
     目的 :构建细粒棘球绦虫疫苗候选分子EgA31重组质粒 ,表达及纯化EgA31 GST融合蛋白 ,为疫苗的研究奠定基础。
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  表达与纯化
     Objective To express and purify the catalase peroxidase katG gene from mycobacterium tuberculosis.
     目的 表达与纯化结核分支杆菌katG 蛋白,为深入研究异烟肼耐药机制奠定基础。
短句来源
     Objective: The aim of this research is to clone,express and purify the AP-2β gene and prepare the antibody.
     目的 :克隆、表达与纯化出AP - 2 β蛋白质 ,并用所表达的蛋白质制备出抗AP - 2 β的抗体。
短句来源
     Objective: To express and purify human ScF v specific against botulinum neurotoxin type A(BoNT/A), and analyze its binding a c tivity.
     目的 :实现特异性抗A型肉毒毒素人源单链抗体 (ScFv)的表达与纯化 ,并进行结合活性分析。
短句来源
     Objective To express and purify the fusion protein dsRed tagged OVA epitope antigen via construction an expression vector pET16bOR.
     目的构建红色荧光蛋白(dsRed)与卵白蛋白(Ovalbumin,OVA)表位融合蛋白的高效表达质粒,并表达与纯化此红荧光蛋白标记的OVA模式抗原。
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  “express and purify”译为未确定词的双语例句
     Aim To express and purify the fusion protein of the extracellular region of 9.1C3/LAIR 1 molecule with Fc fragment of human IgG1 and to prepare the monoclonal antibody (mAb) against the cxcellular region of 9.1C3/LAIR 1 expressed in the eukaryotic cells.
     目的 表达并纯化 9.1C3/LAIR 1胞膜外区与人的IgG1Fc段的融合蛋白 ,制备针对 9.1C3/LAIR 1胞膜外区的单克隆抗体 (mAb)。
短句来源
     Objective: To clone the mecA fragment which encodes the transpeptidase domain of penicillin binding protein 2a(PBP2a) of methicillin resistant Staphylococcus aureus(MRSA),to express and purify the transpeptidase domain of PBP2a.
     目的:对编码耐甲氧西林金黄色葡萄球菌(MRSA)青霉素结合蛋白2a(PBP2a)转肽酶区的mecA基因片段进行克隆、表达、纯化及鉴定。
短句来源
     Objective:To express and purify outer membrane protein 2(Omp2) 167~434 amino acid residues of Chlamydia trachomatis serovar D in E.
     目的:用构建含沙眼衣原体(Chlamydia trachomatis, Ct)血清型D型外膜蛋白2(outer membrane protein 2, Omp2)167~434位氨基酸编码基因(omp2’)的重组表达载体,在大肠杆菌E.
短句来源
     Objective To clone human TRP 1 code gene,express and purify TRP 1 protein.
     目的 克隆TRP 1编码基因 ,表达并纯化TRP 1蛋白。
短句来源
     Objective To construct the prokaryotic expression vector of the fusion protein composed of protein transduction domain-eight arginine (8R) and MUC1 core peptides, and to express and purify the 8R-MUC1 core peptide fusion protein which should possess biological activity.
     目的 :构建蛋白质转导结构域 8个精氨酸聚合体 (8R)与 MUC1核心肽融合蛋白的原核表达载体 ,并表达、纯化出具有生物学活性的 8R- MUC1核心肽融合蛋白。
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  express and purify
There was a clear correlation between the number of predicted transmembrane domains in a given target and its propensity to express and purify.
      
We are exploring procedures to express and purify large numbers of prokaryotic membrane proteins.
      
Work continues in order to express and purify the recombinant PmTLP for use in antifungal assays.
      
We have also, for the first time, been able to express and purify a His-tagged a2-AR.
      
With this approach, we were able to express and purify 67 recombinant proteins representing 66% of the annotated genes.
      
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Recombinant human interferon gamma (rHu IFN-γ) produced in E. co-li carrying plasmid pBVIFN-γ or pIFN-γ was purified to homogeneity with recoveries of 30% and 25% respectively, The purified rHu IFN-y appeared on SBS-PAGE electrophoresis as a single band of MW 17.5. kD The specific activity of the purified r huIFN-γ reached 7.56 × 107IU/mg protein. N terminal amino acid sequencing showed that the protein expressed and purified was rHuIFN-γ.

本文对两个重组人γ干扰素高效表达株pIFN-γ及PBVIFN-γ的表达产物进行了纯化并对纯化的γ干扰素进行了活性鉴定及N端氨基酸序列分析。采用连续沉淀的方法对高表达菌株pBVIFN-γ及低表达菌株pIFN-γ,裂解液进行初步纯化,然后应用单克隆抗体亲和层析方法进行纯化,分别可纯化14倍与933倍,均达电泳纯,回收率分别为25%和30%,比活性达7.56×10~7U/mg蛋白。SDS-PAGE电泳上γ干扰素分子量约17.5kD。测定了纯化的γ干扰素N末端19个氨基酸序列,与由其DNA序列推导的氨基酸序列完全一致,确认了本研究所表达、纯化的γ干扰素达到了较高纯度。本方法为γ干扰素的批量生产奠定了基础。

Human ciliary neurotrophic factor(hCNTF)was expressed and purified from E.coli.Thecoding sequence of hCNTF gene was cloned in the expression vector pBV220.The E.coli strainDH 5α transformed with pBV220/hCNTF expressed high level(about 50 % by gel scanning)ofhCNTF,which was insoluble in E.coli and was contained within inclusion bodies.It was showedthat the best condition for washing inclusion bodies was 2 mol/L urea.hCNTF waschromatographed on Sephacryl S-200 column at high concentration of detergent,then...

Human ciliary neurotrophic factor(hCNTF)was expressed and purified from E.coli.Thecoding sequence of hCNTF gene was cloned in the expression vector pBV220.The E.coli strainDH 5α transformed with pBV220/hCNTF expressed high level(about 50 % by gel scanning)ofhCNTF,which was insoluble in E.coli and was contained within inclusion bodies.It was showedthat the best condition for washing inclusion bodies was 2 mol/L urea.hCNTF waschromatographed on Sephacryl S-200 column at high concentration of detergent,then on DEAE-Sepharose column at low concentration of detergent.After these two chromatographicsteps,the purity of prepared hCNTF is about 98%.hCNTF was biologically active after refold-ing in air.The promoting effects of hCNTF on the survival and development of cultured E10chick DRG neurons and spinal motor neurons were also significantly demonstrated.

人睫状神经营养因子(hCNTF)克隆入pBV220中,在DH5α菌株中表达,重组蛋白以包含体的形式存在,表达量为菌体总蛋白的50%左右。经比较发现用2mol/L脲洗涤包含体可溶解大量可溶性细菌蛋白,且包含体损失较小。在高浓度变性剂条件下进行sepharcylS-200凝胶过滤,解决了纯化中hCNTF易聚合的问题,在低浓度变性剂条件下进行DEAE离子交换,有利于蛋白活性的保持。经两步纯化后得到均一性hCNTF,纯度达95%以上。在自然状态下使hCNTF复性。纯化复性后的hCNTF对无血清培养的鸡胚背根节神经元和脊髓腹角运动神经元有明显的维持存活和促进生长发育的生物效应。

human neutralizing monoclonal antibody Fab fragments to the glycoprotein G1 and a human Fab antibody to the nucleocapsid protein (NP) of hantaan virus have been developed by using phage display technique. The human IgG Fab genes of heavy and light chains were amplified from a HFRS patient in convalescent phase. The combinatorial phage antibody library was prepared by inserting both heavy and light chain Fab genes into phagemid vector pComb3 and followed by the help of phage infection. The library was selected...

human neutralizing monoclonal antibody Fab fragments to the glycoprotein G1 and a human Fab antibody to the nucleocapsid protein (NP) of hantaan virus have been developed by using phage display technique. The human IgG Fab genes of heavy and light chains were amplified from a HFRS patient in convalescent phase. The combinatorial phage antibody library was prepared by inserting both heavy and light chain Fab genes into phagemid vector pComb3 and followed by the help of phage infection. The library was selected by panning phages which expressed the specific human antibody Fabs on their surface. Total 5 rounds of panning, with alternately using the purified hantaan virus virions and the non-neutralizing Mabs captured glycoproteins, were performed and the specific human Fab fragments to hantaan virus were selected and expressed in bacteria. The protein specificity of the expressed human derived monoclonal antibody Fab fragments were characterized by immune precipitation of radiolabeled hantaan viral proteins, three of the expressed human Fabs showed the G1 specificity and another one was specific to NP of hantaan virus. The specific bindings of the Fab antibodies to hantaan virus were demonstrated by their specific reactions with hantaan virus in ELISA and IFAT. The expressed and purified human Fabs to hantaan virus G1 protein clearly showed the neutralizing activity to HTN virus in plaque reduction neutralization test (PRNT). The results provide poten-tial promise for future use in the prophylaxis or therapy of serious HFRS caused by hantaan virus.

运用噬菌体表面表达(Phagedisplay)技术,获得人源中和性抗汉滩病毒汉滩型G1基因工程单克隆抗体(单抗)Fab段基因及其表达,并同时获得抗汉滩病毒核蛋白(NP)的Fab抗体。从肾综合征出血热疫区恢复期病人抗凝血中分离到的外周淋巴细胞中,提取了总细胞RNA。通过RT-PCR方法,用一组人IgGFab基因特异性引物,从合成的cDNA中经PCR扩增了一组轻链和重链Fab段基因,将轻链和重链先后插入噬菌体载体pComb3,成功地建立了抗汉滩病毒抗体基因库,并用纯化的汉滩病毒颗粒及抗汉滩病毒糖蛋白鼠单抗捕捉糖蛋白抗体原的方法,对此抗体库进行了富集筛选,在短期内成功地获得了抗汉滩病毒核蛋白和糖蛋白G1的人源单抗Fab段基因,并在大肠杆菌中获得有效表达。核苷酸序列分析证实,所获得的基因为人源IgGFab基因。用特异性放射免疫沉淀(IP)、IFAT和ELISA以及空斑减少中和试验鉴定表明,表达的人Fab抗体能识别汉滩病毒结构蛋白,其中抗G1人Fab抗体具有体外中和活性。人源抗汉滩病毒基因工程抗体的获得,为今后可能的临床应用提供了良好前景。

 
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