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free pollens
相关语句
  游离花粉
     The developmental process of microsporogenesis,the number of pollens in anther and the number of free pollens of the photoperiod sensitive male sterile Millet under long day cycles (LD,>15 h) and short day cycles (SD,=10 h) were investigated.
     对光敏雄性不育谷子 (Setaria italica) 6 83在长日 (>1 5 h)和短日 (=1 0 h)下小孢子的发育途径、花药中花粉总数及游离花粉总数作了观察和统计。
短句来源
     The average number of pollens per anther and free pollens per field of version under long day cycles and short day cycles were 66.84,13.73 (under LD) and 111.86,20.1 (under SD) respectively.
     每花药中花粉平均数 ,长日条件下为 6 6 .84,短日照下为 1 1 1 .86 ,差异显著。 每视野中的游离花粉平均数 ,长日条件下为 1 3 .73 ,而在短日条件下为 2 0 .1。
短句来源
  相似匹配句对
     Analysis of Free Amino Acid in Pollens Collected by Bees
     油菜、蚕豆、柿子、梅花、紫云英和玉米等六种蜂花粉的游离氨基酸分析
短句来源
     On f-free Subsets
     关于f-free子集
短句来源
     Free design
     自由的设计
短句来源
     TEM Observation of the Pollens
     花粉的透射电镜观察
短句来源
     30% for polyvalent pollens.
     夏秋花粉占30%,
短句来源
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The panicles of rice with microspore at the late uninucleated stage were collected and pretreated at 8-10℃ for 10 days. The isolated pollen grains were prepared as follows: (1) After preculturing the anthers on agar or liquid medium for 2-7 days, the pollen grains were separated by the crush methods (Nitsch, 1974) or by magnetic stirring. The debris was removed, and prepared at a concentration of 2.5 × 105 microspores per ml. (2) By inoculating anthers on liquid medium, they would float on the surface...

The panicles of rice with microspore at the late uninucleated stage were collected and pretreated at 8-10℃ for 10 days. The isolated pollen grains were prepared as follows: (1) After preculturing the anthers on agar or liquid medium for 2-7 days, the pollen grains were separated by the crush methods (Nitsch, 1974) or by magnetic stirring. The debris was removed, and prepared at a concentration of 2.5 × 105 microspores per ml. (2) By inoculating anthers on liquid medium, they would float on the surface of the medium and the free pollen grains would continuously liberate into the medium from the anthers periodically. On transferring the anthers to a fresh mediem, the free pollen grains shedded from the anthers at times were collected. These isolated microspores were incubated in a shallow layer of liquid medium. The media used for culture were Miller or N6 basal medium supplemented with ser-ine, 100 mg; glutamine, 800 mg and m-inositol 5g per litre.The androgenesis of the cultured microspores in vitro was observed. The isolated pollen grains precultered for more than three days could divide continuously and develop into multicellular masses and calli, while those non-precultured did not undergo the first mitosis. More calli were produced from the cultures prepared by the second mitosis. When these cutures were transferred to differentiation medium, some of them differentiated intact plants.When the culture medium was free from the three organic additives or one of them as mentioned above, the further growth of the cultures was suppressed. It is interesting to note that when the medium contained metabolites of anther tissues, the growth of cultures could be significantly enhanced.

对花粉处于单核晚期的粳稻稻穗先进行10天10℃的低温处理,然后用以下方法制备花粉进行游离花粉粒培养:(1)将花药接种在固体或液体培养基上预培养2—7天,分别用挤压法和磁力搅拌法分离提取花粉;(2)利用液体漂浮培养的花药自然连续释放花粉的特点,于接种后第3、7、10天分离出不同时间释放的花粉。培养基为Miller或N_6培养基补加丝氨酸100mg/1,谷氨酰胺800mg/1与肌醇5g/1两种方法制备经3天以上预培养的花粉都能持续分裂形成多细胞团与愈伤组织。尤其是第二种方法制备的花粉形成了大量愈伤组织。一部分愈伤组织转入分化培养基后分化出完整的小植株。 对游离花粉粒培养所需求的培养基成分与小孢子发育动态进行了研究。

Tobacco flower buds at mid-binucleate pollen stage were cold-treated and their anthers were then float-cultured to release the pollen, which were subsequently macerated in enzyme solution for a short time. And de-exined pollen grains were eventually isolated. The main factors affecting artificial germination of the de-exined pollen were investigated, including osmotic pressure of the enzyme solution during the isolation process, polyethylene glycol (PEG) or sucrose in the culture medium...

Tobacco flower buds at mid-binucleate pollen stage were cold-treated and their anthers were then float-cultured to release the pollen, which were subsequently macerated in enzyme solution for a short time. And de-exined pollen grains were eventually isolated. The main factors affecting artificial germination of the de-exined pollen were investigated, including osmotic pressure of the enzyme solution during the isolation process, polyethylene glycol (PEG) or sucrose in the culture medium as well as supplementation of lactoalbumin hydrolysate (LH) . Finally, a medium containing D2 macroelements, 30% PEG-6000 and 0.1% LH was established, which supported the de-exined pollen to germinate well with a frequency up to 57 .8 % . After 24 h of culture, the generative cell in more than half of the pollen tubes, already divided into two sperms. Using a method of micro-suspension droplets with the aid of a small piece of filter paper, 30 to 40 de-exined pollen grains were pollinated onto the stigma, resulting in nearly half of the pollen tubes growing in the style and approximately a yield of one seed out of four de-exined pollen grains after subsequent ovary culture. The seeds were germinated into seedlings. The artificial germination of de-exined pollen can be fur- ther used as a tool for understanding the role of exine in pollen germination. The in vitro pollination with such exine-free pollen might become a new means for introducing foreign genes into the seeds and offsprings.

通过花蕾低温处理、花药漂浮培养与花粉短时酶解程序可脱去花粉外壁,分离出烟草(Nicotianatabacum L.)的脱外壁花粉。研究了分离过程中的酶液渗透压、培养基中聚乙二醇(PEG)与蔗糖以及添加水解乳蛋白等因素对脱外壁花粉人工萌发的影响。在含30%PEG-6000与0.1%水解乳蛋白的D_2培养基中,萌发率最高达57.8%;花粉管生长正常,培养24h后一半以上的花粉管中生殖细胞分裂成精子。用微滴和贴滤纸小片的方法将30~40粒脱外壁花粉授予柱头上,近一半能萌发花粉管并在花柱中生长。采取授粉后子房培养方法,获得了种子与幼苗。从而建立了脱外壁花粉离体授粉实验系统。讨论了脱外壁花粉人工萌发与离体授粉实验系统的建立对于研究外壁在花粉萌发中的生物学功能以及开拓新的转基因技术等方面的意义。

The developmental process of microsporogenesis,the number of pollens in anther and the number of free pollens of the photoperiod sensitive male sterile Millet under long day cycles (LD,>15 h) and short day cycles (SD,=10 h) were investigated.Some special abortive types were detected in the sporogenous cells or microspore mother cells,60%~80% of sporogenous cells or microspore mother cells shrank,and their structure was destroyed,they developed into abnormal black clumps at last.The average number of pollens...

The developmental process of microsporogenesis,the number of pollens in anther and the number of free pollens of the photoperiod sensitive male sterile Millet under long day cycles (LD,>15 h) and short day cycles (SD,=10 h) were investigated.Some special abortive types were detected in the sporogenous cells or microspore mother cells,60%~80% of sporogenous cells or microspore mother cells shrank,and their structure was destroyed,they developed into abnormal black clumps at last.The average number of pollens per anther and free pollens per field of version under long day cycles and short day cycles were 66.84,13.73 (under LD) and 111.86,20.1 (under SD) respectively.The relationship between the appearance of abnormal black clumps and decline of pollen number was analyzed.The results suggested that the appearance of abnormal black clump might cause microspore abortion.

对光敏雄性不育谷子 (Setaria italica) 6 83在长日 (>1 5 h)和短日 (=1 0 h)下小孢子的发育途径、花药中花粉总数及游离花粉总数作了观察和统计。发现长日条件下 ,小孢子败育主要发生在造孢细胞和花粉母细胞时期 ,此时 ,有 6 0 %~ 80 %的药室的造胞细胞和花粉母细胞严重收缩 ,细胞结构破坏 ,演变成着色极深的“异常黑块”(埃氏苏木精染色 )。每花药中花粉平均数 ,长日条件下为 6 6 .84,短日照下为 1 1 1 .86 ,差异显著。每视野中的游离花粉平均数 ,长日条件下为 1 3 .73 ,而在短日条件下为 2 0 .1。研究对“异常黑块”的出现与花粉总数大幅度降低的关系作了分析 ,并讨论了“异常黑块”发生的可能原因及其与小孢子败育的关系

 
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