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anti hd
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  抗-hd
     synthetic HDAg 27 peptide which was selected and designed by the authors and synthesized by Shanghai Institute of Biochemistry, Chinese Academy of Science was used with ELISA method to de-tect serum anti HD in HBV infected subjects in Chongqing.
     采用中科院上海生化所合成丁型肝炎病毒(HOV)抗原27肽酶联法,检测重庆地区乙型肝炎病毒(HBV)感染者中血清丁型肝炎病毒抗体(抗-HD)结果:300例献血员中1例阳性(此人ALT较正常升高2倍)113例甲型肝炎、58例非乙型肝炎均为阴性;
短句来源
     When the natural HDAg was used to compete anti HD in four anti HD positive and two anti HD negative serum specimens,anti HD was negative in all specimens. It is shown that the HDAg 27peptide has natural HDAg activity capable of being recognized by natural anti HD and is a new diagnostic agent being more simple,save,stable and reliable.
     采用天然丁型肝炎病毒抗原(HOAg)与之竞争抗-HD,4份阳性转为阴性,2份阴性结果不变,提示本HDV抗原合成肽具有天然HDAg活性,可特异识别抗-HD,是一新的、稳定、安全、可靠的诊断用试剂。
短句来源
  抗hdv
     A synthetic HDAg 27 oligopeptide , which was selected and designed by the authors andsynthesized by Shanghai Institute of Biochemistry , Chinese Academy of Science was used with EIAmethod to detect serum anti HD in different population at eight hospitals in China.
     采用自行设计、选择含天然HDAg部分片段的人工合成27肽建立EIA检测抗HDV方法,在国内8家医院检测各类人员中血清抗HDV状况。 HDV抗原合成肽由中国科学院上海生物化学研究所合成。
短句来源
     These results were in coincidence with the previ-ous repotrs from the same unit. The detection rates of anti HD were different in different areas. The coin-cidence rate was 94.9%(74/78 ) when compared with Abbott EIA kit.
     同一单位抗HDV的检出与已往用市售试剂结果基本一致,与Abbott试剂符合率为94.9%(74/78)。
短句来源
     The results suggested that theHDAg 27 peptide possessed the activity of the natural HDAg,and it could be recognized by natural ant- HD. Therefore, it may be used as a diagnostic agent for detecting anti HD.
     提示本HDV抗原27肽具有HDV抗原活性,能特异地为天然抗HDV识别,可作为检测抗HDV诊断试剂。
短句来源
  “anti hd”译为未确定词的双语例句
     The results revealed that HDV RNA was detected in 20/20 of the positive serum samples for HDAg and 6/10 of the positive samples for anti HD(negative HDAg),while HDV RNA was negative in 10 positive samples for HBsAg,HBeAg and Anti HBc.
     结果:20例丁型肝炎病毒抗原阳性的HDVRNA均为阳性,10例丁型肝炎病毒抗体阳性中HDVRNA阳性6例,10例单纯HBsAg、HBeAg和抗HBc阳性的均未检出HDVRNA。
短句来源
  相似匹配句对
     Anti-H.
     抗H .
短句来源
     Anti-H.
     方法:将H.
短句来源
     DETECTION OF Anti-HD IN THE POPULATION OF GUANGDONG PROVINCE
     抗-HD在广东人群中检出
短句来源
     Of the five samples,three were positive forIgG-anti HD.
     其中 3例为抗HD-IgG阳性患者。
短句来源
     Results HD was efficient.
     结果  HD疗效满意。
短句来源
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  anti hd
Various tests for anti-HD have been developed, and some are available commercially.
      
The binding was inhibited by an anti-hD1D2 in a dose-dependent manner.
      
Only 3 of the 20 early serum specimens scored anti-HD positive by the commercial assay.
      


Aflatoxin B_1(AFB_1)is a very powerful carcinogen and mutagen.After the cornand the food are naturally contaminated by AFB_1,an effective substance which candetoxify AFB_1 but keep the corn and the food eatable,hasn't been found so far.Weshould identify whether antimycotoxin—HD(AntiHD)may be spread as an effectivetoxinicide.The mutagenicity of AFB_1 treated with antimycotoxin—HD was assayedwith the Salmonella/microsome test.S.typhimurium strains TA98 and TA100 were used as the standard...

Aflatoxin B_1(AFB_1)is a very powerful carcinogen and mutagen.After the cornand the food are naturally contaminated by AFB_1,an effective substance which candetoxify AFB_1 but keep the corn and the food eatable,hasn't been found so far.Weshould identify whether antimycotoxin—HD(AntiHD)may be spread as an effectivetoxinicide.The mutagenicity of AFB_1 treated with antimycotoxin—HD was assayedwith the Salmonella/microsome test.S.typhimurium strains TA98 and TA100 were used as the standard test strains.The liver homogenate fraction(S-9)from rat was induced with a polychlorinatedbiphenyl mixture(Aroclor 1254).Results from spot test and incorporation assay wereas follows:The revertant colonies for TA98 per plate induced by antimycotoxin—HDtreated AFB_1 and untreated AFB_1 were 34±3.1((?)±S_(?))VS.714±30.8 respectively.The difference was highly significant(p<0.001).The revertant colonies for TA100per plate induced by antimycotoxin—HD treated AFB_1 and untreated AFB_1 were138±9.8 VS.885±94.3 respectively.The difference was highly significant(p<0.001).The spontaneous revertant colonies per plate were 23.7±3.2 for TA98 and101.9±6.99 for TA100.Although AFB_1 treated with antimycotoxin—HD yieldeda negative result in the Salmonella/microsome test,(the number of induced revertantscompared to that of the spontaneous was less than 2fold),there existed a statisticaldifference between the number of spontaneous revertants per plate and that of inducedrevertants per plate in presence of Antimycotoxin-HD treated AFB_1,the latterwas a little more than the former(p<0.01).According to the dose-response curveof AFB_1,the degradation rate of AFB_1 treated with antimycotoxin—HD was esti-mated at 99.19% for TA98 or 98.20% for TA100.The data obtained suggestthat the mutagenicity of AFB_1 is essentially nullified by the effect of antimycoto-xin—HD.

本文报道用沙门氏菌/微粒体实验检测法证明:抗霉毒剂HD可使黄曲霉霉素B_1的致突变性丧失。

The procedures of efficiently preparingbiotin-labeled HDV cDNA probe, whichhad been used for detecting HDV RNA (he-patitis del?a virus RNA) from serum, weredescribed. Using the probe, the minimalamount (50 pg) of HDV RNA could bedetected by spot hybridization. Twenty serum samples were collectedfrom the hepatitis patients, who were po-sitive for IgG type antibody to HDV (IgG-anti HD) in the sera or positive for HDVantigen in the liver tissue for the test. Al-though the sera had been stored for 1-2years,...

The procedures of efficiently preparingbiotin-labeled HDV cDNA probe, whichhad been used for detecting HDV RNA (he-patitis del?a virus RNA) from serum, weredescribed. Using the probe, the minimalamount (50 pg) of HDV RNA could bedetected by spot hybridization. Twenty serum samples were collectedfrom the hepatitis patients, who were po-sitive for IgG type antibody to HDV (IgG-anti HD) in the sera or positive for HDVantigen in the liver tissue for the test. Al-though the sera had been stored for 1-2years, frozen and thawed repeatedly, HDVRNA were still detected from the sera offive patients with chronic active hepatitis.Of the five samples,three were positive forIgG-anti HD.The fact that HDV RNA couldstill actively replicate even though IgG-antiHD had appeared in the sera suggestedthat it might be not true.The IgG-anti HDwas protective antibody. This result is of significance for clinicalas well as epidemiological studies.

运用分子生物学技术,在国内首次成功地制备了生物索标记丁型肝炎病毒cDNA探针,建立了HDVRNA 的血清斑点杂交技术。HDV cDNA 重组质粒对大肠杆菌 HB101的转化率达10~5转化子/μgDNA;生物素化11-dUTP掺入率达33%;斑点杂交检测低限为50pg。在20例虽曾被反复冻融过、HDV感染标志物阳性患者血清中,仍检出5例慢性活动性肝炎患者HDV RNA阳性;其中 3例为抗HD-IgG阳性患者。提示血清中的抗HD-IgG也许并不一定是保护性抗体,出现后仍可能存在HDV病毒的活跃复制。

Serum anti-HD antibody was detected by using ELISA method in Wuhan children.The subjects includrd 40 healthy children,8 patients with acute hepatitis A,14 non-hepatitis cases and 120 cases of chronic HBV infection.All cases of first three groups were sero-negative.Anti-HD IgG prevalence was 17.5% in the last group.Of those,15.4% was in chronic HBsAg carriers,18.5% in chronic persistent hepatitis B cases.There was no significant difference between them.The above findings suggest that some...

Serum anti-HD antibody was detected by using ELISA method in Wuhan children.The subjects includrd 40 healthy children,8 patients with acute hepatitis A,14 non-hepatitis cases and 120 cases of chronic HBV infection.All cases of first three groups were sero-negative.Anti-HD IgG prevalence was 17.5% in the last group.Of those,15.4% was in chronic HBsAg carriers,18.5% in chronic persistent hepatitis B cases.There was no significant difference between them.The above findings suggest that some chronic HBV infected children in Wuhan had HDV infection at the same time.

采用酶联免疫竞争法,对武汉地区儿童血清中丁型肝炎病毒抗体(抗-HD)进行检测,32例健康儿童、8例急性黄疸型甲型肝炎、14例非肝炎病儿,抗-HD检测结果全部阴性,120例慢性HBV感染儿童,抗-HD阳性率17.5%,其中慢性HBsAg携带者15.4%(6/39),慢性迁延性肝炎18.5%(15/81),两组无明显差异。提示本地区一部分慢性HBV感染儿童,合并HDV感染,值得引起注意。

 
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