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two substrates
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  双底物
     From the research of the reaction between two substrates(F6P and PP i) and the relationship between product(P i)and substrates(F6Pand PP i),a sequential enzyme mechanism with two substrates and two products are proposed preliminarily for the PFP from Brassica napus.
     从两底物 F6 P和 PPi 的相互作用以及产物磷酸 (Pi)与底物 (F6 P和 PPi)的相互关系分析 ,初步推断油菜籽 PFP的催化反应为双底物双产物的有序机制。
短句来源
     This can be effectively solved by blocking the pathways leading to the byproducts that are of no benefit to 1,3-propanedil production. The 1,3-propanediol titers of 36.09 g 1-1 and 45.22 g 1-1 have been obtained by using two-stage fermentation with two substrates and microaerobic fermentation in the fed-batch culture of Klebsiella pneumoniae M5al, respectively, which greatly exceeded the level that can be achieved in China.
     运用双底物两步法和微氧法对Klebsiella pneumoniae M5al菌株进行补料批式培养,发酵液中1,3-丙二醇浓度分别可达36.09gl~(-1)和45.22gl~(-1),较国内所能达到的水平有很大提高。
短句来源
     The optimal pH was between 7.5~8.3,and the relationship between the anaerobic ammonia oxidation rate and pH can be described by two substrates and two inhibitors model during pH 7.0~9.0.There was substrata competition between the heterotrophic denitrification bacteria and ANAMMOX bacteria when organics in the influent.
     最佳pH值为7.5~8.3,在pH值为7.0~9.0之间,厌氧氨氧化速率与pH值之间的关系可以用双底物双抑制剂模型描述; 厌氧氨氧化污泥中存在着异养反硝化菌,有机物的存在会导致其与厌氧氨氧化菌之间的基质竞争.
短句来源
  “two substrates”译为未确定词的双语例句
     the Km,Vmax of the two substrates, 4-coumaroyl-CoA and malonyl-CoA were respectively 1.93μM,4.84μM/min and 9.8μM, 4.95μM/min, so 4-coumaroyl-CoA was the optimal substrate of RS.
     两种底物香豆酰辅酶A、丙二酰辅酶A的K_m、V_(max)值分别为1.93μM,4.84μM/min和9.8μM,4.95μM/min,由此可知香豆酰辅酶A为RS的最适底物;
短句来源
     When the reaction was carried out for 2 .5h at 120℃, ΔA/A0 for the two substrates increased to 1.69 and 1.2 times of the original values, respectively.
     在120℃温度下反应2.5h,月见草油的ΔA/A0值增加1.69倍,包络物的ΔA/A0值增加1.2倍。
短句来源
     La 2-2xSr 1+2xMn 2O 7 single phase thin films are grown on two substrates with different latlice parameters.
     双层钙钛矿结构的La2 - 2XSr1 +2XMn2 O7(X =0 .32 )单相薄膜生长在具有不同晶格参数的两种衬底上 .
短句来源
     the molar ratio of two substrates is 1: 1 ,reaction temperature is 136℃-138℃ ,reaction time is 1h,the yield is 75.6%.
     实验优化结果为:反应物摩尔比1:1,反应温度136-138℃,反应时间1 h,收率75.6%。
     After 18 days of bioleaching, the removal rates of Zn and Cu could reach 89.2% and 56.5% respectively in the treatment of mixing and inoculation of these two substrates;
     经过18d的生物淋滤,在2种底物配合并接种的处理中,Zn和Cu的去除率分别可达89.2%,56.5%;
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  相似匹配句对
     Two.
     二、末日意识
短句来源
     Two.
     二.
短句来源
     Study on Enzymolysis of Cellulase in Two Different Substrates
     一株纤维素酶酶学特性的研究
短句来源
     The optimal concentrations of these two substrates were 5g/L.
     以淀粉为底物时,最佳初始pH值7.5,最大产氢能力为166mL/g,最佳底物浓度均为5g/L.
短句来源
     concentration of substrates;
     底物浓度;
短句来源
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  two substrates
Functional analysis in vitro showed that the mutant enzyme almost completely lacked the catalytic activity towards two substrates, progesterone and 17-hydroxyprogesterone.
      
grown on a mixture of two substrates nonequivalent in terms of bioenergetics (ethanol + glucose) was shown.
      
When two substrates were oxidized by the mitochondria with the alternative CN-resistant oxidase (AO) inhibited with salicylhydroxamic acid, the rate of NADH oxidation in state 4 dropped by over 40% as compared to the initial rate.
      
The dispersion equation for quasi-Rayleigh acoustic modes in transversely isotropic sandwich-type structures (an elastic layer between two substrates) has been obtained in the analytical form.
      
The temperature dependency of the retention index and the retention index increments between the two substrates are characteristic for a certain type of isomers.
      
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(1) Sodium salt of reduced codehydrogenase I has been obtained in good yield as a dry powder from codehydrogenase I by reduction with alcohol and alcohol dehydrogenase. This preparation was stable for at least 5 months when kept dry at -15℃. (2) The properties of the particle-bound codehydrogenase I cytochrome reductase system in heart muscle preparation were found to differ considerably from those of the soluble enzyme as obtained by Mahler et al. Among other things, the affinity for cytochrome c of the particle-bound...

(1) Sodium salt of reduced codehydrogenase I has been obtained in good yield as a dry powder from codehydrogenase I by reduction with alcohol and alcohol dehydrogenase. This preparation was stable for at least 5 months when kept dry at -15℃. (2) The properties of the particle-bound codehydrogenase I cytochrome reductase system in heart muscle preparation were found to differ considerably from those of the soluble enzyme as obtained by Mahler et al. Among other things, the affinity for cytochrome c of the particle-bound enzyme is much greater than the soluble enzyme. The Michaelis constant for cytochrome c of the former is only one twelfth of that of the latter.(Fig. 2A). (3) With either oxygen or excess cytochrome c as electron acceptor, it was found that the overall activity, in terms of rate of oxygen consumption or cytochrome c reduction, when both succinate and reduced codehydrogenase I were oxidized simultanously, did not represent the sum of the rates of oxidation when these two substrates were separately oxidized but equalled only the faster of the two separate oxidation rates(Fig. 5, Tables 1, 2). If 2,6-dichlorophenol indophenol was used as the electron acceptor, the overall rate of simultaneous oxidation of these two substrates was found to equal exactly the sum of the rates of separate oxidation(Table 3). (4) When either oxygen or excess cytochrome c was used as the electron acceptor, reduced codehydrogenase I and succinate each inhibited the rate of oxidation of the other(Figs 4, 6 & 7). Evidence has been presented to show that the inhibition of succinate oxidation by reduced codehydrogenase I is not due to the accumulation of oxaloacetate. (5) When malonate was also added to the reaction mixture, succinate no longer produced any inhibition of the oxidation of reduced codehydrogenase I(Fig. 8). (6) It is therefore concluded that in heart muscle preparation both succinate and reduced codehydrogenase I are oxidized by cytochrome c through a common, velocity limiting factor. This is in accordance with the view previously reached by some workers from studies on the action of certain inhibitors. However, it should be noted that in our experiments no agents which might produce any conceivable change in the colloidal structure of the enzyme system has been employed. (7) It should be emphasized that our results clearly show that great caution must be exercised in drawing conslusion on the role an enzyme might play in a complex enzyme system from studies of the properties of a solubilized enzyme. (8) It is believed that the competition of two enzyme systems for a common linking factor as demonstrated in this report has provided a new method for studies on the mutual relations of two or more enzyme systems.

(一)本報告提供了一個從輔酶Ⅰ,用酶還原法製備還原輔酶Ⅰ的方法。我們所製得的還原輔酶Ⅰ鈉鹽乾粉,可以在低温保存數月而不被氧化。 (二)與心肌製劑中顆粒相結合的輔酶Ⅰ細胞色素還原酶系,和用乙醇抽出的水溶性的輔酶Ⅰ細胞色素還原酶的性質頗不相同。其中比較重要的不同點是對於細胞色素c的親力,前者遠大於後者,其米氏常數僅約為後者的十二分之一。 (三)用一心肌顆粒製劑作為材料,無論用氧或過量之細胞色素c作為氫受體,還原輔酶Ⅰ與琥珀酸同時氧化時的總速度,不等於二者分別氧化時速度之和,而僅等於其中氧化較快者單獨氧化時之速度。但如用[2,6]二氯靛酚作為氫受體時,二者共同氧化時之總速度完全等於二者分別氧化時速度的和。 (四)當用氧或過量之細胞色素c作為氫受體時,琥珀酸與還原輔酶Ⅰ能彼此互相抑制對方氧化的速度。有足夠的實驗材料說明,還原輔酶Ⅰ對於琥珀酸氧化的抑制,不是由於草醯乙酸聚集的緣故。 (五)如果在反應混合物中同時含有琥珀酸脫氫酶的專一抑制劑,丙二酸,則琥珀酸對於還原輔酶Ⅰ氧化作用的抑制即被解除。 (六)根據以上的實驗結果,可以認為,還原輔酶Ⅰ及琥珀酸先通過一個共同的因子與細胞色素c作用。這個共同的因子在一般情形之下,也是...

(一)本報告提供了一個從輔酶Ⅰ,用酶還原法製備還原輔酶Ⅰ的方法。我們所製得的還原輔酶Ⅰ鈉鹽乾粉,可以在低温保存數月而不被氧化。 (二)與心肌製劑中顆粒相結合的輔酶Ⅰ細胞色素還原酶系,和用乙醇抽出的水溶性的輔酶Ⅰ細胞色素還原酶的性質頗不相同。其中比較重要的不同點是對於細胞色素c的親力,前者遠大於後者,其米氏常數僅約為後者的十二分之一。 (三)用一心肌顆粒製劑作為材料,無論用氧或過量之細胞色素c作為氫受體,還原輔酶Ⅰ與琥珀酸同時氧化時的總速度,不等於二者分別氧化時速度之和,而僅等於其中氧化較快者單獨氧化時之速度。但如用[2,6]二氯靛酚作為氫受體時,二者共同氧化時之總速度完全等於二者分別氧化時速度的和。 (四)當用氧或過量之細胞色素c作為氫受體時,琥珀酸與還原輔酶Ⅰ能彼此互相抑制對方氧化的速度。有足夠的實驗材料說明,還原輔酶Ⅰ對於琥珀酸氧化的抑制,不是由於草醯乙酸聚集的緣故。 (五)如果在反應混合物中同時含有琥珀酸脫氫酶的專一抑制劑,丙二酸,則琥珀酸對於還原輔酶Ⅰ氧化作用的抑制即被解除。 (六)根據以上的實驗結果,可以認為,還原輔酶Ⅰ及琥珀酸先通過一個共同的因子與細胞色素c作用。這個共同的因子在一般情形之下,也是這兩個酶系統的速度限制因子。應該指出在我們的實驗中,並未使用任何可能影響酶系統結構的條件,因此我們的結果是在一個比較接近於生理狀態的情形之下獲得的。 (七)應該着重指出,從本報告的結果可以看到,一個用人為的方法從複雜酶系上溶解下來的酶的性質,有時並不能代表這個酶在有組織的酶系統中的真實情况。 (八)我們相信,本報告所說明的兩酶系競爭一個共同因子的一些現象,將为研究複雜酶系之間的相互關係,提供一個新的方法。

This paper concerns the application of an immunoenzymatic techniqueperformed on formalin-fixed and paraffin-embedded liver-egg sections of mice infected with Schistosoma japonicum for the diagnosis of schistosomlasis.Antibodies wete detected by the technique in 109 serum samples from patients with schistosomiasis,The positive rate was as high as 95.41% and the false positive rate of 141 uninfected control sera was 1.42%.It was shown that the method described is sensitive and specific enough to meet the requirement...

This paper concerns the application of an immunoenzymatic techniqueperformed on formalin-fixed and paraffin-embedded liver-egg sections of mice infected with Schistosoma japonicum for the diagnosis of schistosomlasis.Antibodies wete detected by the technique in 109 serum samples from patients with schistosomiasis,The positive rate was as high as 95.41% and the false positive rate of 141 uninfected control sera was 1.42%.It was shown that the method described is sensitive and specific enough to meet the requirement of the diagnosis of the disease.In the experiment,it has been made to improve the stain procedure of samples which is described by Mesulam(1976)and compare the resultant color developed by these two substrates.

本研究应用福尔马林固定的感染鼠肝石蜡切片免疫酶染色法。检测109例血吸虫病病人血清,阳性率达95.41%;141例正常人血清的假阳性率为1.42%,表明该法有较高的敏感性及特异性,具有一定的诊断价值。实验中并对所应用的底物及有关步骤作了比较及改进。

Zn is one of the accepor dopants for getting the hightest efficient radiate recombination centers in GaN. It is reported that the different Zn concentration in Zn-doped GaN can provide emission colors of blue, green, yellow and red.So the problem non-uniformity of Zn concentration in the layer if GaN by VPE must be solved. Monemar et at. have showed that Zn concentration was related to the position of substrate in a reactor chamber. High ohmic resistance Zn-doped GaN layer was not at the front of reaction zone...

Zn is one of the accepor dopants for getting the hightest efficient radiate recombination centers in GaN. It is reported that the different Zn concentration in Zn-doped GaN can provide emission colors of blue, green, yellow and red.So the problem non-uniformity of Zn concentration in the layer if GaN by VPE must be solved. Monemar et at. have showed that Zn concentration was related to the position of substrate in a reactor chamber. High ohmic resistance Zn-doped GaN layer was not at the front of reaction zone but at some distance of the reaction zone. A typical variation in resistivity was a factor of 40 when the substrats were arranged in a region of 15 mm along the reaction zone. This behavior can partly be explained by the vari-arion of N-vacancy concentration in this growth direction. We have investigated a lot of samples of Zn-doped GaN. It was observed that the concentration of Zn was non-uniform in the VPE layers of GaN.Variant position for obtaining ohmic Zn doped GaN was different even on the same sample surface, resulting in different emission spectrum.This phenomena may connect with defects of grown GaN such as helical dislocation different morphology and growing pits on the GaN epitaxy layer surface.Zn concentration was found to be non-uniform in direction along the reaction zone. This is in accord with the result given by Monemar et al. basically.This behavior can be explained as follows. Acoording to the "diffusion controlled-boundary layer theory" the flux of a component Zn is described as ,where D is the diffusion constant;P, the pressure;R,the gas constant; T,the absolute temperature; δ, the effective thickness of the boundary layer and PZn0, PZn, the partial pressure of a component Zn at the boundary layer and the gas-solid interface respectively. If both the substrates a and b are placed in the growth region 3 to 5 cm downstream from the GaCl outlet (as shown in Table 1) then component Zn got to two substrates a and b can be des- cribed aswhen the reaction starts, PZn(a) = PZn(b). According to the formula δ(x) = (ηx/ρv)1/2·C the result calculated is δ(a) = 0.98δ(b) Checking (2) aganist (3), numerical values of JZn)(a) and JZn(b) may depend on PZn0(a) and PZn0(b). If PmZn is the Zn partial pressure of Zn outlet, la, and lb are distances of PZnm from substrates a and b respectively, then JZn(a) and JZn(b) are proportional to PZnm/la and PZnm/lb respectively. Because la>lb (Fig. 8 ) then JZn(b)>JZn(a). This is the reason why Zn concentration increases with the increase of the distance from the Gad outlet.Reactor cell structure was designed on the basis of analysis as aforementioned. Vapor flow was regulated as PZn/(a) = PZn0(b)= PZnm. Many experiments naive shown that Zn concentrations are similar on substrates a and b. The spectra of PL, CL, EL of the same kind of samples of Zn-doped GaN are similar too. On a φ25 GaN slice φ2mm Al dots were evapored every other 2mm. Each of them was measured. The difference in the wavelength of EL spectral peaks is less than 10 A.In conclusion, our experiments indicate that the electric parameters of n-GaN grown on two types of bracket and in different distances are similar; and their difference is no more than one order of magnitude. This result contradicts with that of Monemar .It is shown that the non-uniformity of Zn concentration on the doped GaN layer in our experiments is not caused by the difference of N-vacancy but rather the unreasonable distribution of Zn vapor in the reaction zone.

在Ar-HCl-Ga-NH_3气相外延生长GaN的过程中,分析了Zn在GaN中分布不均匀的原因,认为除生长出晶体完整性好的n-GaN提供均匀掺杂的必要条件外,Zn气流的合理分布是获得Zn均匀掺入的关键。我们通过设计新的Zn喷口,能在φ25mm的GaN:Zn样品上,得到了Zn的均匀分布,PL、CL、EL均为一种颜色,室温下测定了EL光谱,不同点的峰值偏差小于10A。

 
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