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Hemorrhage of Grass Carp is very disadvantageous to fisheries products, causing a mortality of 50-70% for 1 year old juvenile fish. The pathogen of hemorrhage of Grass Carp was studied in the early fifties. During 1978-1984, the Reovirus was isolated, which was named Grass Carp Reovirus (GCR).Observation under electron microscope of the tissues of kideny, spleen, liver, blood vessel, intestine and muscle of disease fish shows that there were two kinds of virosome in the pathological cells. The diameter... Hemorrhage of Grass Carp is very disadvantageous to fisheries products, causing a mortality of 50-70% for 1 year old juvenile fish. The pathogen of hemorrhage of Grass Carp was studied in the early fifties. During 1978-1984, the Reovirus was isolated, which was named Grass Carp Reovirus (GCR).Observation under electron microscope of the tissues of kideny, spleen, liver, blood vessel, intestine and muscle of disease fish shows that there were two kinds of virosome in the pathological cells. The diameter of one particle was 80-100 nm and the other 20-30 nm. The main pathological (infested) cells contained endothelial cell of capillaries, reticuloendothelial cell, eosinophil leucocyte, pericyte and interstitial cell as well as other epithelioid cells. Since the endothelial cell of capillaries were damaged and lysed, hemorrhagic symptoms appeared in the tissue.According to the testing of viruses which were isolated and purified, the bigger one belonged to Reovirus while the other was temporarily defined as Picornaviridae. 用电子显微镜观察草鱼(Ctenopharyngodon idellus C.et v.) 出血病的病理切片,在病变细胞中发现有两种病毒颗粒,一种是呼肠孤病毒,以前已有报道;另一种为直径仅20—30nm的小病毒颗粒,系首次在鱼类细胞中发现。两种病毒主要侵染病鱼血管及微血管内皮细胞。内皮细胞受损而至解体,红细胞弥散细胞间隙,导致出血症状。此外还侵染肾上皮细胞,肾间质细胞、肠上皮细胞以及脾网状细胞、嗜酸性白细胞等。根据小病毒颗粒的形态及核酸分析,拟初步鉴别为小RNA病毒科病毒。 The synovial folds of knee joint of 6 normal and pathological cases were observed with a Hitachi S-450 SEM. The normal synovial fold had a smooth surface with clear villi, and the cell projections were filiform or round; while the pathological synovial fold showed a roung surface, on which the villi were more slender disorderly arranged and even compressed, and in some places the pathological cells adhered together. 用扫描电镜观察了6例正常和病变的成人膝关节滑膜襞。正常滑膜襞表面光洁,圆柱状绒毛清晰,细胞突起呈网丝状或圆柱状,病变滑膜襞则表面粗糙.绒毛变细,排列紊乱,并有受压及局部粘连现象. The gastrin concentrations in esophagus cancer tissuse and its surrounding non-neoplastic mucosae and in fasting serum from 40 patients with esophagus cancer were measured by radioimmunoassay. Meanwhile , G-IR(gastrin-immunoreactive) cells in the tissues were examined by immunohistochemical study. Control study was carried out in 20 normal people.The results showed that no difference was seen between the mean fasting serum gastrin concentrations, between the mean contents of gastrin in the cancer tissues... The gastrin concentrations in esophagus cancer tissuse and its surrounding non-neoplastic mucosae and in fasting serum from 40 patients with esophagus cancer were measured by radioimmunoassay. Meanwhile , G-IR(gastrin-immunoreactive) cells in the tissues were examined by immunohistochemical study. Control study was carried out in 20 normal people.The results showed that no difference was seen between the mean fasting serum gastrin concentrations, between the mean contents of gastrin in the cancer tissues for the patients with esophageal tumors derived from different regions of the esophagus or of varrant pathological cell types(P>0.05).And there was no difference in the mean gastrin levels compared to that of control, either(P>0.05). The contents of gastrin in the cancer tissues and its surrounding non-neoplastic tissues did not exceed each other(P>0.05); No relationship was found between the gastrin contents in paraneoplastic mucosae and dysplasia or local metastases of cancer cells. But close positive correlation existed between the gastrin contents in the normal esophagus mucosae(r=0.927) or the esophageal lesions(r=0.906) and the corresponding fasting serum gastrin concentrations(P<0.005). No G-IR cell was found in all the normal esophagus mucosae,the esophageal lesions and its surrounding non-neoplastic mucosae by immuno-histochemical method.In conclusion :①The esophagus cancer did not affect the secretion of gastrin and the fasting serum concentration of the patients.②No differentiation of G-IR cells was seen during the carcinogenesis and development of esophageal cancer. ③No evidence of gastrin-au-tocrine was found in esophageal lesions. 本文应用放射免疫分析方法和免疫组织化学方法对40例食管癌患者的癌组织及距癌边缘1cm、3cm和5cm处癌旁粘膜组织内及血清中胃泌素含量和胃泌素型(G-IR)细胞进行了观察,并用20例正常对照进行了比较研究。结果表明,不同起源部位、不同组织类型的食管癌患者,其空腹血清胃泌素浓度之间、癌组织胃泌素含量之间均无明显差异(P>0.05),而且与正常对照之间也无显著差异(P>0.05)。癌组织与癌旁粘膜组织内的胃泌素含量之间无显著差异(P>0.05)。癌旁粘膜组织内胃泌素含量与不典型增生、癌细胞浸润均无关。而正常人食管粘膜胃泌素含量与血清胃泌素浓度之间(r=0.927)、食管癌组织内胃泌素含量与血清胃泌素浓度之间(r=0.906)均为正相关关系(P<0.005)。免疫组化染色显示正常人食管粘膜、食管癌及癌旁粘膜组织内均未见G-IR细胞。作者认为:①食管癌组织本身对患者的胃泌素分泌及血清胃泌素浓度无明显影响;②食管癌在癌变的发生发展过程中未见有G-IR细胞的分化;③食管癌未见有自分泌现象。
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