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envelope glycoprotein gene
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  “envelope glycoprotein gene”译为未确定词的双语例句
     Isolation and Identification of Pseudorabies Virus La Strain and Nucleotide Acid Comparison of Envelope Glycoprotein Gene E
     伪狂犬病病毒La株的分离鉴定和囊膜糖蛋白E基因的克隆及序列分析
短句来源
     Molecular Cloning and Sequence Identification of the Envelope Glycoprotein Gene E_2 in NADL Strain of Bovine Viral Diarrhea Virus
     牛病毒性腹泻病毒NADL株囊膜糖蛋白E_2基因的克隆与序列鉴定
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     Methods Two envelope glycoprotein gene fragments were cloned from the recombinant plasmid pHXB2 containing the human immunodeficiency virus 1(HIV 1) proviral genome of HXB2 isolate.
     方法从人免疫缺陷病毒1(HIV-1)HXB2分离株原病毒基因组的重组质粒pHXB2中克隆了两段膜糖蛋白基因(ENV)片段。
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     Immunogenicity of envelope glycoprotein gene of reticuloendotheliosis virus expressed in insect cell
     昆虫细胞表达的网状内皮组织增殖症病毒囊膜糖蛋白及其免疫性
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     Expression of Hantavirus Z10 Envelope Glycoprotein Gene G2 Recombinant and Its Application in IFA
     汉坦病毒G2糖蛋白在昆虫细胞中的表达及其在免疫诊断中的应用
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  相似匹配句对
     Progress on Envelope Glycoprotein D of Pseudorabies virus
     伪狂犬病病毒囊膜蛋白gD研究进展
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     Secreted Expression of D2V Envelope Glycoprotein in Pichia pastoris
     登革2型病毒E蛋白在酵母菌中的分泌表达
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     Promoter Activities in the Baculovirus Envelope Glycoprotein gp64 Gene
     杆状病毒囊膜糖蛋白gp64基因启动子活性分析(英文)
短句来源
     Expression of the Envelope Glycoprotein D Gene of Pseudorabies Virus Ea Strain
     伪狂犬病病毒鄂A株囊膜蛋白gD基因在杆状病毒载体系统中的表达研究
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     The White Envelope
     白色的信封
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  envelope glycoprotein gene
The phylogenetic tree of 27 CSFV sequences based on the complete envelope glycoprotein gene (Erns-E2) displayed better resolution than that based on the complete open reading frame.
      
Porcine reproductive and respiratory syndrome virus (PRRSV) strains from 13 states in the United States, Guatemala and Canada were used to compare the envelope glycoprotein gene (ORF 5) nucleotide and deduced amino acid sequences.
      
Nucleotide and encoded amino acid sequences of their envelope glycoprotein gene revealed 98.8 and 99.8% identity, respectively.
      


A cDNA fragment of newcastle disease virus(NDV)strain F48E8 containing partial fusion(F)gene and partial haemagglutinin neuraminidase(HN)gene was obtained by RT PCR.The amplified fragment was 926 bps long as expected analyzed by agarose gel electrophoresis.It was directly cloned into plasmid vector pUC19 and the positive clone named as p926 was confirmed by restriction endonuclease analysis.Then the cDNA fragment was labeled as a DIG probe...

A cDNA fragment of newcastle disease virus(NDV)strain F48E8 containing partial fusion(F)gene and partial haemagglutinin neuraminidase(HN)gene was obtained by RT PCR.The amplified fragment was 926 bps long as expected analyzed by agarose gel electrophoresis.It was directly cloned into plasmid vector pUC19 and the positive clone named as p926 was confirmed by restriction endonuclease analysis.Then the cDNA fragment was labeled as a DIG probe for detection of the envelope glycoprotein genes of NDV.The specificity of the DIG probe was tested by RNA dot blot hybridization.The probe detected the genomic RNA of three NDV strains(F48E8,La Sota,Ulster),and no hybridization signal was observed with RNA extracted from other avian viruses including IBDV and IBV.The clones(pF and pHN)containing envelope glycoprotein genes of NDV strain F48E8 were tested in a Southern hybridization assay with the DIG probe.The result showed that the 1.7kb long cDNA fragment of pF and the 1.93kb long cDNA fragment of pHN were positive,and further confirmed that the F and HN glycoprotein genes of NDV strain F48E8 were successively cloned.

应用RT-PCR获取新城疫病毒(NDV)F48E8株的部分囊膜糖蛋白基因片段。扩增产物经琼脂糖凝胶电泳分析,长为926bp,与预期结果相符;将该基因片段定向克隆入质粒载体pUC19中,得到重组质粒p926,酶切分析结果与已发表的NDV毒株酶切位点一致。随后将该基因片段标记成为地高辛探针,该探针能检测出三株NDV(F48E8株、LaSota株、Ulster株)基因组RNA,不能检测出IBDV和IBV基因组RNA,表明其特异性良好。应用该探针进行Southern杂交试验鉴定NDVF48E8株的F和HN基因克隆,结果质粒pF内1.7kb片段和质粒pHN内1.93kb片段为阳性,从而进一步证实NDVF48E8株的囊膜糖蛋白基因均已成功克隆。

Objective To acquire enough envelope glycoproteins so as to facilitate a further study of the structure and function of envelope glycoproteins from various kinds of virus isolates. Methods Two envelope glycoprotein gene fragments were cloned from the recombinant plasmid pHXB2 containing the human immunodeficiency virus 1(HIV 1) proviral genome of HXB2 isolate. Two corresponding expression plasmids pYENV1 and pYENV2 were constructed using a yeast shuttle inducible expression vector pYES2 Furthermore...

Objective To acquire enough envelope glycoproteins so as to facilitate a further study of the structure and function of envelope glycoproteins from various kinds of virus isolates. Methods Two envelope glycoprotein gene fragments were cloned from the recombinant plasmid pHXB2 containing the human immunodeficiency virus 1(HIV 1) proviral genome of HXB2 isolate. Two corresponding expression plasmids pYENV1 and pYENV2 were constructed using a yeast shuttle inducible expression vector pYES2 Furthermore plasmid pYENVG12 with the fused gene cassette between external envelope gene fragment and β lacZ gene was constructed. These three plasmids were transformed into the unicellular eukaryotic S. cerevisiae BJ1991 and the galactose induced transformants were analyzed with the SDS PAGE. Results The result showed that a 50kD specific protein induced from the transformant containing the fused plasmid was shown to have antigenic reactivity with HIV 1 antibodies positive sera by immunodetection. Conclusions Therefore not only could the expression of the antigenic fragment be directly indicated by the β galactosidase activity, but also it laid some foundation for further purification of the fragment of the expressed envelope glycoproteins.

目的为获得足够量的膜糖蛋白,以便于对不同HIV分离株膜糖蛋白的结构与功能进行进一步的研究。方法从人免疫缺陷病毒1(HIV-1)HXB2分离株原病毒基因组的重组质粒pHXB2中克隆了两段膜糖蛋白基因(ENV)片段。以酵母穿梭诱导表达质粒pYES2为载体,构建了两个相应的重组表达质粒pYENV1和pYENV2;进一步利用大肠杆菌β-半乳糖苷酶基因(β-lacZ)构建了HIV-1膜外糖蛋白DNA片段与β-lacZ基因的融合表达质粒。将此3种质粒分别转化单细胞真核生物酿酒酵母BJ1991,得到的转化子经半乳糖诱导表达后进行菌体全蛋白的SDS-PAGE分析。结果克隆的基因片段在酿酒酵母中产生了分子质量为50×103的特异性诱导蛋白;对含此融合表达质粒的酵母转化子半乳糖诱导后表达产物的免疫检测表明,与对照菌株相比,融合表达产物具有和HIV-1阳性血清抗体反应的抗原性。结论可通过β-半乳糖苷酶活性的测定直接指示抗原片段的表达;为表达的膜糖蛋白片段的进一步分离纯化打下了一定基础

Objective To construct hantavirus Z37 envelope glycoprotein genes G1 and G2 recombinant and express in eukaryocyte. Methods Using three pairs of primers based on hantavirus Z37 M gene sequences. PCR products F1, F2 and G2 were obtained by PCR from the Plasmid pGEMZ37(containing partial cDNA of G1) and Plasmid pCUMZ37(containing partial cDNA of G1 and whole cDNA of G2). G1 PCR products were also obtained by fusing F1 and F2 PCR products. The G1 and G2 PCR products were then digested by BamHⅠ and...

Objective To construct hantavirus Z37 envelope glycoprotein genes G1 and G2 recombinant and express in eukaryocyte. Methods Using three pairs of primers based on hantavirus Z37 M gene sequences. PCR products F1, F2 and G2 were obtained by PCR from the Plasmid pGEMZ37(containing partial cDNA of G1) and Plasmid pCUMZ37(containing partial cDNA of G1 and whole cDNA of G2). G1 PCR products were also obtained by fusing F1 and F2 PCR products. The G1 and G2 PCR products were then digested by BamHⅠ and XhoⅠ and cloned into the corresponding sites of expression vector pcDNA3.1 respectively. The recombinants pcDNA3.1 G1 and pcDNA3.1 G2 were identified by digestion with endonuclease BamHⅠ and XhoⅠ and confirmed by sequencing. After transfecting COS 7 cells by Calcium phosphate/DNA precipitating method, indirect immunofluorescence assay (IFA) was used to verify the transient expression of HV Z37 G1 or G2 protein in COS 7 cells. Results The recombinant expression plasmids pcDNA3.1 G1 and pcDNA3.1 G2 were constructed. After transfection with the above recombinant expression plasmids, specific antigens were detected within cells by IFA. Conclusions The reombinant expression plasmids pcDNA3.1 G1、pcDNA3.1 G2 were constructed successfully and expressed transiently in eukaryocyte.

目的 构建汉坦病毒浙 37(Z37)株包膜糖蛋白基因G1、G2真核表达质粒 ,并在真核细胞中表达。方法 根据Z37M基因序列设计 6条引物 ,分别以质粒pGEMZ37、pCUMZ37为模板 ,通过聚合酶链反应 (PCR)获得G1及G2片段。将G1、G2片段经BamHⅠ、XhoⅠ双酶切后插入至真核表达载体 pcDNA3 .1(+) ,经酶切鉴定 ,并测序证实。以磷酸钙沉淀法分别将重组质粒转染COS 7细胞 ,用间接免疫荧光法 (IFA)检测瞬时表达的蛋白。结果 获得分别含有编码汉坦病毒 (HV)Z37株包膜糖蛋白G1、G2基因的重组质粒 pcDNA3 .1 G1、pcDNA3 .1 G2 ;在转染的COS 7细胞内 ,用IFA可检测到细胞内有特异性荧光。结论 成功地构建了HVZ37株包膜糖蛋白G1、G2基因真核表达载体 ,并可在COS 7细胞中瞬时表达。

 
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