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dna polymerase genes
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  dna聚合酶基因
     It was proposed that the mitochondrial DNA polymerase genes in primitive eukaryotes might be obtained from eubacteria, in plants, might derive from plasmid, and in fungi/animals, might be of T 3/T 7 related phage origin.
     原始真核生物线粒体DNA聚合酶基因可能来源于细菌 ,植物线粒体DNA聚合酶基因可能从质粒获得 ,而真菌和动物线粒体DNA聚合酶基因可能起源于T3 /T7相关噬菌体。
短句来源
     In this study, the DNA polymerase genes of 9 different pathotypes strains were compared, to analyze the relationship between the pathotype and MDV’s DNA polymerase gene. These strains included four international reference strains—vaccine strain CVI988, virulent strain GA, very virulent strain Md5 and very virulent plus strain 648A;
     为了分析马立克氏病病毒(MDV)致病型与其DNA聚合酶基因的关系,本研究比较了9个不同致病型的该基因的同源性关系,这包括四种不同致病型的国际参考株即弱毒疫苗株CVI988/Ripens株、强毒株GA、超强毒株Md5和特超强毒株648A;
短句来源
     Analysis reveals that,at different evolutional stages of eukaryotes, mitochondrial DNA polymerase genes might originated from diverse organisms through lateral gene transfer.
     分析显示 :在真核生物演化的不同阶段 ,线粒体DNA聚合酶基因可能通过水平基因转移方式起源于不同类群的生物。
短句来源
  “dna polymerase genes”译为未确定词的双语例句
     Using a common upstream primer and two type-specific downstream primers from the HSV DNA polymerase genes in one PCR mixtures, both types of HSV DNA could be amplified and produced products with different sizes(HSV-Ⅰ 543 bp and HSV-Ⅱ 372 bp).
     在HSV-Ⅰ、Ⅱ两型的DNA多聚酶基因上设计了一条两型共同的上游引物(HDP-B)和两条型特异的下游引物(HDP-1、HDP-2)。
短句来源
     This set of primers allows amplification of a selected fragment of the four kinds of HV family (CMV,HSV Ⅰ,HSV Ⅱ,and EBV)DNA polymerase genes, while the viruses were accurately identified by the molecular weight and the enzymolysis analysis of their amplified products. The assay was applied to the peripheral blood lymphocyte from 33 patients with neonatul jaundice. The results showed that the HV DNA were defectable in 13 of the 39 neonatal jaunolice samples.
     结果13例外周血淋巴细胞检出疱疹病毒DNA,总检出率333%,其中巨细胞病毒(CMV)11例,Ⅱ型单纯疱疹病毒(HSVⅡ)2例,检出率分别为282%(11/39)和51%(2/39),未检出Ⅰ型单纯疱疹病毒(HSVⅠ)和EB病毒(EBV)DNA。
短句来源
     More and more drug-resistant HSV strains have been reported recently. The mutations in thymidine kinase and DNA polymerase genes are proved to be the main mechanism of drug-resistance.
     大量研究证明,胸苷激酶和DNA聚合酶缺陷是导致单纯疱疹病毒耐药的主要机制。
短句来源
  相似匹配句对
     coli DNA polymerase I, E.
     用E。
短句来源
     Base Selection by E. colt DNA Polymerase I
     大肠杆菌DNA聚合酶Ⅰ的“碱基选择”作用
短句来源
     Differentiation Effects on DNA Polymerase Beta and Its Associated Genes' Expression in Eca-109 Cells
     分化诱导剂对Eca-109细胞DNA聚合酶β及其相关基因表达的影响
短句来源
     DNA was extracted from the cells and the drug-resistant genes were detected by polymerase chain reaction (PCR).
     从转基因小鼠骨髓细胞提取DNA,用PCR检测转基因小鼠骨髓细胞耐药基因的表达;
短句来源
     Suppression subtractive hybridization for cloning of genes transactivated by RNase H protein of HBV DNA polymerase
     应用抑制性消减杂交技术筛选HBV DNA聚合酶中RNase H的反式调节基因
短句来源
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  dna polymerase genes
Cladograms of iridoviruses were inferred from bootstrap analysis of molecular data sets comprising all published protein and DNA sequences of the major capsid protein, ATPase and DNA polymerase genes of members of the Iridoviridae family Iridovirus.
      
Phylogenetic analysis of the DNA polymerase genes of these viruses suggests that EhV belongs to a new genus within the family of algal viruses, Phycodnaviridae.
      
Assuming a common cellular origin of viral DNA polymerase genes the corresponding amino acid sequences were chosen to construct a phylogenetic tree showing the relatedness among large DNA viruses of eucaryotes.
      
Detection of New DNA Polymerase Genes of Known and Potentially Novel Herpesviruses by PCR with Degenerate and Deoxyinosine-Subst
      
Enhanced expression of α-type DNA polymerase genes reduces AZT cytotoxicity in hamster tr5 cells
      
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A simple and rapid polymerase chain reaction (PCR) procedure was developed for simultaneous detecting and genotyping of herpes simplex virus (HSV) in our laboratory. Using a common upstream primer and two type-specific downstream primers from the HSV DNA polymerase genes in one PCR mixtures, both types of HSV DNA could be amplified and produced products with different sizes(HSV-Ⅰ 543 bp and HSV-Ⅱ 372 bp).And the difference in molecular mass enabled us to discriminate the type of HSV by electrophoresis.A...

A simple and rapid polymerase chain reaction (PCR) procedure was developed for simultaneous detecting and genotyping of herpes simplex virus (HSV) in our laboratory. Using a common upstream primer and two type-specific downstream primers from the HSV DNA polymerase genes in one PCR mixtures, both types of HSV DNA could be amplified and produced products with different sizes(HSV-Ⅰ 543 bp and HSV-Ⅱ 372 bp).And the difference in molecular mass enabled us to discriminate the type of HSV by electrophoresis.A total of 5 HSV strains were examined by this method, and the results were consistent with those determined by virus isolation and serological methods.No specific amplification was observed when using other kinds of DNAs as ternplate.The method is sensitive to detect 1fg of HSV DNA.

本文利用PCR技术建立一种对HSV直接基因分型的方法。在HSV-Ⅰ、Ⅱ两型的DNA多聚酶基因上设计了一条两型共同的上游引物(HDP-B)和两条型特异的下游引物(HDP-1、HDP-2)。三条引物共同组成一个扩增反应体系,在HSV-Ⅰ产生543bp条带,HSV-Ⅱ产生372bp条带,据此在基因水平上对HSV进行分型。5株不同来源的HSV(2株Ⅰ型,3株Ⅱ型)分型结果与病毒分离及血清学方法完全一致。该反应体系与其它来源的DNA不产生特异反应,敏感性可达1fg。应用该法对151份临床可疑HSV感染的标本进行检测并分型,结果与免疫学方法完全一致。

method for the detection and direct typing of HSV by antigen capture PCR(AC-PCR) technique has been developed. One type-common upstream primer and twotype-specific downstream primerswere prepared to amplify DNA from the HSV type 1 ortype 2 DNA polymerase gene. HSV-1 or HSV-2was capturec by anti-HSV gC and gDMcAbs coating to 0.5ml polypropylene microcentrifuge tubes. Using these three primerssimultaneously in the AC-PCR reaction mixtures, both types of HSV DNA were amplified toproduce products...

method for the detection and direct typing of HSV by antigen capture PCR(AC-PCR) technique has been developed. One type-common upstream primer and twotype-specific downstream primerswere prepared to amplify DNA from the HSV type 1 ortype 2 DNA polymerase gene. HSV-1 or HSV-2was capturec by anti-HSV gC and gDMcAbs coating to 0.5ml polypropylene microcentrifuge tubes. Using these three primerssimultaneously in the AC-PCR reaction mixtures, both types of HSV DNA were amplified toproduce products of different sizes. By direct gel analysis, the products of standard HSV-1and HSV-2 strains had the predictive sizes of 477 and 399bp, respectively, and difference inmolecular weight enabled us to type the HSVstrain. The products amplified from samples ofHSV-1 strain 17syn ̄+ and HSV-2 Sav werecloned into pUC18 plasmids and sequenced.The result of DNA sepuences indicates that theAC-PCR assay is specific. The sensitivity ofAC-PCR is very high, 10pfu HSV-1 or 1pfu HSV-2 can be detected and typed.

用抗单纯疱疹病毒(HSV)型共同性gC和gD羊克隆抗体(McAb),包被即Eppendorf管,捕捉HSV,同时加入3个引物:一个是HSV─1/HSV─2型共同性上游引物,另两个分别是HSV─1和HSV─2型特异性下游引物。借此建立了能直接分型检测HSV的抗原捕获聚合酶链式反应(AC─PCR)。HSV─1的扩增产物为477bp,HSV─2的为399bp两型病毒经AC─PCR扩增后产生分子量不同的DNA片段,致使AC─PCR能直接分型检测HSV。HSV─1和HSV─2扩增产物的克隆和序列分析表明,本方法特异性好。用本法检测Balb/c幼鼠中枢神经系统HSV感染的脑标本,进一步证实本方法不仅敏感、特异,而且分型准确。

Thermostable DNA polymerase gene had been amplified from Thermus aquaticus YT-1 using PCR technique. The amplified 2. 5kb DNA fragement was inserted into pUCl8 and confirmed to be thermostable DNA polymerase gene by restriction mapping and DNA sequencing. The insert fragement was then combined into an expression vector, pBV221. This recombinant plasmid overexpressed a 94kDa of recombinant protein in E. colt. 100ml of E. colt culture could yield 1. 5X 105 units of Taq DNA polymerase,...

Thermostable DNA polymerase gene had been amplified from Thermus aquaticus YT-1 using PCR technique. The amplified 2. 5kb DNA fragement was inserted into pUCl8 and confirmed to be thermostable DNA polymerase gene by restriction mapping and DNA sequencing. The insert fragement was then combined into an expression vector, pBV221. This recombinant plasmid overexpressed a 94kDa of recombinant protein in E. colt. 100ml of E. colt culture could yield 1. 5X 105 units of Taq DNA polymerase, which could be applied in the PCR.

用PCR法从水生栖热菌菌株YT-1中扩增耐热DNA聚合酶基因,得到2.5kb的DNA片段,扩增片段重组到pUC18中测序证实为Taq DNA聚合酶基因,将该片段重组到pBV221温控表达质粒中,在大肠杆菌中表达出94kDa的重组蛋白,100ml培养物的细胞产酶为1.5×10~5u,表达的蛋白能催化PCR反应的进行。

 
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