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maintenance hemodialysis patients mhd
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     56 on maintenance hemodialysis.
     其中维持性血液透析(HD)患者56例,接受肾替代治疗前(ND)患者27例。
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     The characteristics of maintenance hemodialysis in the elderly.
     老年维持性血液透析的特点和分析
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     Psychological status in maintenance hemodialysis patients.
     维持性血透患者的心理状态研究
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     Nutritional status of the maintenance hemodialysis patients
     维持性血液透析病人的营养状况
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     Nursing of the exercise of patients with maintenance hemodialysis
     维持性血液透析患者运动的护理
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Plasma interleukin 1β(IL-1β) bioactivity was measured by the augmentation of proliferation of murine thymocytes in 9 non-dialyzed uremic patients. 12 maintenance hemodialysis patients (MHD) and 9 normal individuals. Results showed, plasma IL-1β bioactivity in urema and MHD patients was without significance compared with normals'. Suggested, it was no sensitive marker to show the production and secretion of cytokine in urema and MHD patients by measuring plasma...

Plasma interleukin 1β(IL-1β) bioactivity was measured by the augmentation of proliferation of murine thymocytes in 9 non-dialyzed uremic patients. 12 maintenance hemodialysis patients (MHD) and 9 normal individuals. Results showed, plasma IL-1β bioactivity in urema and MHD patients was without significance compared with normals'. Suggested, it was no sensitive marker to show the production and secretion of cytokine in urema and MHD patients by measuring plasma cytokine level.

采用胸腺细胞增殖法检测了9名正常人、9名尿毒症未透析者及12名维持性血透(MHD)患者血浆白细胞介素1β(IL-1β)的生物活性。结果表明,尿毒症及MHD患者的血浆IL-1β活性与正常对照组相比无明显差异(P>0.05)。提示血浆细胞因子水平不能完全反映尿毒症及MHD患者细胞因子产生和分泌的情况。

Objective:To observe the effect of Jinshuibao capsule on apoptosis and expression of FasL on peripheral blood T cells in maintenance hemodialysis patients(MHD).Methods:Annexin V and FasL of T cells cultured with on stimulus for 24 hours were measured by flow cytometry in 30 hemodialysis patients(hemodialysis group)and 30 hemodialysis patients both Jinshuibao capsule for three months(Chinese medicine group).Results:Annexin Ⅴ and FasL of T cells significantly...

Objective:To observe the effect of Jinshuibao capsule on apoptosis and expression of FasL on peripheral blood T cells in maintenance hemodialysis patients(MHD).Methods:Annexin V and FasL of T cells cultured with on stimulus for 24 hours were measured by flow cytometry in 30 hemodialysis patients(hemodialysis group)and 30 hemodialysis patients both Jinshuibao capsule for three months(Chinese medicine group).Results:Annexin Ⅴ and FasL of T cells significantly increased in hemodialysis group in comparison with Chinese medicine group,( P <0.05).There was significant positive correlation between Annexin Ⅴ and FasL in both groups ( P <0.05). There was significant negative correlation between Annexin Ⅴ and Ccr ( P <0.05).Conclusion:Jinshuibao can siguificantly inhibit T cell apoptosis in MHD patients.

目的 :观察虫草制剂金水宝对维持性血液透析 (MHD)患者外周血T细胞凋亡和FasL表达的影响。方法 :应用流式细胞仪 (FCM)定量检测了 30名MHD患者 (血透组 )、30名MHD加金水宝治疗 3个月患者 (中药组 )的外周血T细胞在体外无刺激培养 2 4h后AnnexinⅤ和FasL的表达。结果 :血透组T细胞AnnexinⅤ和FasL表达显著高于中药组 (P <0 .0 5 ) ,两组AnnexinⅤ和FasL之间呈显著正相关 (P <0 .0 5 ) ,而AnnexinⅤ与Ccr间呈显著负相关 (P <0 .0 5 )。结论 :金水宝可显著减轻MHD患者的T细胞凋亡

Objective To investigate the effect of different dialysis membranes on peripheral blood transforming growth factor-β1(TGF-β1) and the expression of complement regulatory protein CD59 in maintenance hemodialysis patients(MHD).Methods The levels of peripheral blood TGF-β1 and the expression of CD59 were measured by ELISA and flow cytometry in patients with Cuprophone (CU) and Polysulfone (PSU).Results As compared with normal control group TGF-β1(49.3±6.1) ng/mL, the levels of TGF-β1...

Objective To investigate the effect of different dialysis membranes on peripheral blood transforming growth factor-β1(TGF-β1) and the expression of complement regulatory protein CD59 in maintenance hemodialysis patients(MHD).Methods The levels of peripheral blood TGF-β1 and the expression of CD59 were measured by ELISA and flow cytometry in patients with Cuprophone (CU) and Polysulfone (PSU).Results As compared with normal control group TGF-β1(49.3±6.1) ng/mL, the levels of TGF-β1 in MHD patients were significantly increased, but the levels of TGF-β1in PSU group(64.3±8.3)ng/mL were significantly decreased than that in CU group(81.7±8.7) ng/mL (P<0.01). The activity of CD59 was not expressed in the peripheral blood mononuclear cell of normal person, but it did so in the hemodialysis and non-dialysis group of uremia. CD59 activity was lower in the non- dialysis group, while it was higher in the dialysis group. In CU group it was significantly increased than that in PSU group(P<0.05). There is a positive correlation between TGF-β1 levels and CD59 expression in CU group and PSU group.Conclusion Hemodialysis may activate complement through reaction between blood and dialysis membrane, in- ducing peripheral blood mononuclear cells to synthesize more TGF-β1, while abnormally increased TGF-β1 could increase the activity of CD59, inhibit cells damage caused by abnormal complement activation to protect human body. But different dialysis membrane such as CU and PSU has different effect on TGF-β1 and CD59 in MHD patients. All of these changes are associated with the biocompatibility of the dialysis membrane.

目的 研究不同透析膜对维持性血液透析 (MHD)患者外周血转化生长因子 β1及补体调节蛋白CD5 9活性表达的影响。方法 通过流式细胞术和酶联免疫吸附法测定长期采用铜仿膜 (CU)与聚砜膜 (PSU)透析患者外周血TGF β1水平及CD5 9活性表达。结果 MHD患者血浆TGF β1水平明显增加 (P <0 .0 5 ) ,其中CU组为 (81.7± 8.7)ng mL ;PSU组为 (6 4 .3±8.3)ng mL ,与正常对照组 (49.3± 6 .1)ng mL相比均有显著差异 (P <0 .0 1,P <0 .0 5 ) ;而PSU组与CU组相比 ,外周血TGF β1水平明显下降 (P <0 .0 5 )。CD5 9在正常人外周血单个核细胞中无表达 ,而在尿毒症未透析组及血透组均有表达 ,其中未透析组表达较低 ,而血透组表达较高 ,CU膜血透组较PSU血透组表达要高 (P <0 .0 5 )。经相关分析结果显示 :CU组与PSU组内TGF β1水平与CD5 9表达均呈现良好的相关性 (r=0 .6 4 0 9与 0 .5 83,P <0 .0 5 )。 结论 血液透析通过血液 透析膜间相互反应可活化...

目的 研究不同透析膜对维持性血液透析 (MHD)患者外周血转化生长因子 β1及补体调节蛋白CD5 9活性表达的影响。方法 通过流式细胞术和酶联免疫吸附法测定长期采用铜仿膜 (CU)与聚砜膜 (PSU)透析患者外周血TGF β1水平及CD5 9活性表达。结果 MHD患者血浆TGF β1水平明显增加 (P <0 .0 5 ) ,其中CU组为 (81.7± 8.7)ng mL ;PSU组为 (6 4 .3±8.3)ng mL ,与正常对照组 (49.3± 6 .1)ng mL相比均有显著差异 (P <0 .0 1,P <0 .0 5 ) ;而PSU组与CU组相比 ,外周血TGF β1水平明显下降 (P <0 .0 5 )。CD5 9在正常人外周血单个核细胞中无表达 ,而在尿毒症未透析组及血透组均有表达 ,其中未透析组表达较低 ,而血透组表达较高 ,CU膜血透组较PSU血透组表达要高 (P <0 .0 5 )。经相关分析结果显示 :CU组与PSU组内TGF β1水平与CD5 9表达均呈现良好的相关性 (r=0 .6 4 0 9与 0 .5 83,P <0 .0 5 )。 结论 血液透析通过血液 透析膜间相互反应可活化补体 ,诱导外周血单个核细胞 (PBMC)合成TGF β1增多 ,而增高的TGF β1水平可上调CD5 9基因表达 ,抑制补体异常活化造成的细胞破坏 ,对机体具有代偿性保护意义。然而不同透析膜如CU和PSU对MHD患者TGF β1与CD5 9的影响有所不同 ,这些变化与透析膜的生物相容性有关

 
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