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replicate and express
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  复制和表达
     ConclusionPrimary human fetal hepatocyte is competent for infection with HDV/HBV,HDV can stably replicate and express in human fetal hepatocytes,and at least this replication and expression can reach for 12 days S ASODN can availably inhibit replication and expression of HDV in HDV/HBV infected human fetal hepatocytes.
     结论 HDV在原代培养人胎肝细胞中能稳定复制和表达至少达 12 d; S- ASODN能有效抑制 HDV/ HBV感染人胎肝细胞中 HDV复制与表达。
短句来源
     Therefore, HGV RNA genome can replicate and express in HepG2 cells, this HGV RNA genome transfected cells model could be used as a cell model in the studies of replication and infection of HGV.
     故 HGV RNA基因组能够在HepG2细胞中复制和表达,此细胞模型有可能用于HGV的复制与感染防治的研究。
短句来源
     HCV 1a/1b chimera can replicate and express in HepG2 cells, suggesting that the RNA-dependent RNA polymerase (RdRp)of HCV 1b can initiate the replication of HCV containing genotype 1a untranslated region(UTR) The alterations of 5' UTR of HCV 1b at nucleotides 11, 12, 13, 34 and 35 do not influence its binding to ribosome.
     表明该HCV嵌合体可以在细胞中复制和表达,HCV 1b型的RNA依赖的RNA聚合酶(RdRp)可以起始含1a型非编码区的病毒复制。 HCV 5′端非翻译区第11、12、13、34和35位核苷酸改变可不影响其与核糖体结合。
短句来源
     CONCLUSION Primary human fetal hepatocytes are competent for infection with HBV. HBV can stably replicate and express in HBV-infected human fetal hepatocytes, and at least this replication and expression can last 16 days.
     结论 HBV在原代培养人胎肝细胞中能稳定复制和表达至少达16d。
短句来源
     The results showed that the replicon of E. coli in pMD18T had a high hereditable stability in the passing of Pasteurella multocida at least for 9 generations,which provided a basis for the construction a shuttle expressing plasmid which could replicate and express foreign gene in E. coli and Pasteurella multocida.
     结果表明,pMD18-T中的大肠杆菌复制子能在巴氏杆菌中稳定传递至少9代,这为构建能在禽巴氏杆菌和大肠杆菌中复制和表达外源基因的穿梭表达质粒打下了基础。
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  “replicate and express”译为未确定词的双语例句
     EBV was used to infect these CR2-293 cells, in which EBV could replicate and express EBV antigens.
     用EB病毒感染CR2-293细胞后,23%的细胞可表达EB病毒抗原。 用TPA作用于这些细胞后,表达病毒抗原的细胞数增加。
短句来源
     Conclusion HCV may replicate and express in MT--2 cell for a short period.
     结论HCV可在MT—2中短期复制。
短句来源
     The shuttle plasmid pSE-3 could replicate and express its neomycin resistance in both Streptomyces and E.
     ),所得重组质粒能强启动pIJ486质粒上的氨基糖苷磷酸转移酶基因(aph),并使新霉素抗性基因在大肠杆菌中得到强表达。 此重组质粒被命名为pSE-3,当其转化到变青链霉菌TK54(Tsr(?)
短句来源
     Mitochondria contain their own DNA. They can replicate and express themselves.
     线粒体自身携带DNA,可自我复制、表达。
短句来源
     CONCLUSION: HCV can infect, replicate and express in PBMC.
     结论:PBMC是丙型肝炎病毒肝外复制并表达的场所.
短句来源
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  相似匹配句对
     DESIGN:Design of replicate-measurements.
     设计:重复测量设计。
短句来源
     Global Partial Replicate Computation Partitioning
     全局部分重复计算划分
短句来源
     The replicate method has been proved to be feasible and effective in our practice.
     实践证明,这种复制方法是切实可行、卓有成效的。
短句来源
     CONCLUSION: HCV can infect, replicate and express in PBMC.
     结论:PBMC是丙型肝炎病毒肝外复制并表达的场所.
短句来源
     Conclusions: TTV can infect PBMC and replicate in PBMC.
     结论 :TTV可感染PBMC并在PBMC中复制
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  replicate and express
We engineered a recombinant SIV that lacked the nef gene and had the capacity to replicate and express CD154 on the surface of the infected cells.
      
The potential advantage of such a bacterial vaccine vector is its ability to replicate and express heterologous antigen in vivo.
      
But most RNA viruses, as well as one group of DNA viruses, replicate and express their genome in the cytoplasm.
      


The plasmids pBR325 carrying ampicillin, tetracyline, ehloramphenieol resistance and pUB110 carrying neomycin, kanamycin resistance have been extracted from Es-cherichia coli and Bacillus subtilis respectively, treated with EcoRI endonuclease and ligated with T4 DNA ligase. E. coli and B. subtilis were then transformed with the hybrid plasmid. We have obtained recombinant plasmid pMM1. The hybrid plasmid carries the same antibiotic markers as its parent plasmids except the ehloramphenieol resistance. Its molecular...

The plasmids pBR325 carrying ampicillin, tetracyline, ehloramphenieol resistance and pUB110 carrying neomycin, kanamycin resistance have been extracted from Es-cherichia coli and Bacillus subtilis respectively, treated with EcoRI endonuclease and ligated with T4 DNA ligase. E. coli and B. subtilis were then transformed with the hybrid plasmid. We have obtained recombinant plasmid pMM1. The hybrid plasmid carries the same antibiotic markers as its parent plasmids except the ehloramphenieol resistance. Its molecular weight as measured by gel eleetrophoresis is the total molecular weight of pBE325 and pUB110. Transformation frequency in E. coli is 0.79 × 103 per microgram DNA and 0.15 × 102 in B. subtilis. This recombinant plasmid can be replicated and expressed not only in E. coli but also in. B. subtilis. We have also constructed recombinant plasmid with pBR322 and pUB110, pMM2, and obtained similar results.

将大肠杆菌抗四环素、氨基苄青霉素、氯霉素质粒pBR325和金黄色葡萄球菌/枯草杆菌抗新霉素、卡那霉素质粒pUB110,在体外经限制性内切酶EcoRI和T4 DNA连接酶作用,进行重组,获得了重组质粒pMM 1。pMM 1兼有抗四环素、氨基苄青霉素、卡那霉素和新霉素以及对氯霉素敏感的特性;用凝胶电泳法测定分子量,证明pMM1确为pBR325和pUB110两者的重组质粒。pMM 1在大肠杆菌中的转化频率为每微克DNA 0.79×10~3个转化子,在枯草杆菌受体中为每微克DNA 0.15×10~2个转化子。pMM1不仅能分别在大肠杆菌和枯草杆菌两种受体中复制,而且能同样表达,其抗药性水平为:对氨基苄青霉素不小于每毫升160微克,对四环素不小于每毫升120微克,对新霉素、卡那霉素不小于每毫升80微克。此外,用同样的方法构建了pBR 322和pUB 110的另一个重组质粒pMM 2,也获得了类似的结果。

A multifunction shuttle vector pBE2 has been constructed by using vector pUB110 of Bacillus subtilis and vector pGEG3 of E. coli. It can be replicated and expressed in both Bacillus subtilis and E, coli. There is a strong promoter and a multiple cloning site in vector pBE2, therefore it facilitates gene manipulations in these two host strains.

本文简要地报道了由枯草杆菌运载体pUB_(110)和大肠杆菌运载体pGEM_3构建的一个多功能穿梭载体pBE_2.该载体能在枯草杆菌和大肠杆菌中复制和表达,并具有一个强启动子和一个多酶切位点区可供广泛利用,是一个比较理想的运载体.

A shuttle plasmid vector, pSE-3 was constructed using the Streptomyces plasmid pIJ486 and E. colt plasmid pGEM-3 digested with Bg1 and BamHI respectively. The shuttle plasmid pSE-3 could replicate and express its neomycin resistance in both Streptomyces and E. colt well.The pSE-3 has opposed promoters containing bacteriophage ST6 and T7, the restriction enzyme digestion showed that the pSE-3 has a single site with HindⅢ and EcoRI respectively, it is very useful for the cloning and expression of the valuable...

A shuttle plasmid vector, pSE-3 was constructed using the Streptomyces plasmid pIJ486 and E. colt plasmid pGEM-3 digested with Bg1 and BamHI respectively. The shuttle plasmid pSE-3 could replicate and express its neomycin resistance in both Streptomyces and E. colt well.The pSE-3 has opposed promoters containing bacteriophage ST6 and T7, the restriction enzyme digestion showed that the pSE-3 has a single site with HindⅢ and EcoRI respectively, it is very useful for the cloning and expression of the valuable gene inserts. The pSE-3 is stable in both of Streptomyces and E. coll after re-transformed pSE-3 is re-transformed from Streptomy-ces into E. colt and some 50 generations are propagated.

本文报道了链霉菌和大肠杆菌穿梭质粒载体pSE-3的构建;把具有双启动子的大肠杆菌的质粒pGEM-3与新霉素抗性基因启动子缺失的链霉菌的探针质粒pIJ486分别用BamHI和BglⅡ酶切,T4 DNA连接酶连接后转化到E.coli HB101(Amp(?),Neo(?)),所得重组质粒能强启动pIJ486质粒上的氨基糖苷磷酸转移酶基因(aph),并使新霉素抗性基因在大肠杆菌中得到强表达。此重组质粒被命名为pSE-3,当其转化到变青链霉菌TK54(Tsr(?),Neo(?))的原生质体前,新霉素抗性基因亦能得到强表达。酶切结果表明,构建的具有两个启动子的穿梭质粒载体pSE-3上有HindⅢ和EcoRI的单酶位点,拷贝数约为39。经再转化和传代50代等研究表明,穿梭质粒载体pSE-3在链霉菌和大肠杆菌中均是稳定的。为某些有应用价值的目的基因在大肠杆菌和链霉菌中的克隆与表达提供了一个有价值的穿梭质粒载体。

 
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