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cervical ganglia
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  颈神经节
     The numerical density of HRP-positive neurons in cervical ganglia,anterior horn of spinal cord and facial nucleus in acupuncture group were(2.65±2.39),(2.06±1.30) and(2.43±0.09)×103/mm3 respectively.
     在针灸组中,颈神经节,脊髓前角和面神经核中的HRP阳性神经元的数密度分别为:(2.65±2.39),(2.06±1.30)和(2.43±0.09)×103/mm3。
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  “cervical ganglia”译为未确定词的双语例句
     The postganglionic neurons were mainly located in the stellate ganglion(61.99%), anterior and middle cervical ganglia, and T_2-T_5 ganglia of the sympathetic trunk, bilaterally.
     3.交感节后神经元位于双侧星状神经节(61.99%)和颈前、颈中、T_(2~5)交感干神经节。
短句来源
     RESULTS An inward current was elicited by rapid application of choline in cultured rat superior cervical ganglia neurons with an EC 50 of 8 25 mmol·L -1 ; The choline evoked current consisted of a fast and a slow component and could be significantly inhibited by methyllycaconitine (MLA, 1 μmol·L -1 ) and α bungarotoxin (α Bgt, 1 μmol·L -1 ) which were selective antagonists of α 7 nAChR;
     结果 在培养的大鼠颈上交感节神经元上 ,喷射给予α7 nAChR选择性激动剂胆碱可诱发出一内向电流 ,其EC50 值为 8 2 5mmol·L-1,电流包含快、慢两种成分 ,并能被α7 nAChR选择性拮抗剂methyllycaconitine(MLA ,1μmol·L-1)以及α bungarotoxin(α Bgt,1μmol·L-1)明显抑制 ;
短句来源
     INTERVENTION:After the animals were sacrificed acutely, the superior cervical ganglia(SCG)together with their preganglionic nerve trunks were isolated rapidly. The ganglia were perfused continuously with a Krebs solution[saturated with 950 mL/L O2 and 50 mL/L CO2 ,pH 7.4 ±0.05,(35±0.5) ℃].
     干预:大鼠急性处死后,迅速将颈上神经节连同节前神经离体,用950mL/LO2和50mL/LCO2混合气饱和的,pH为7.4±0.05的克氏溶液,恒温(35±0.5)℃持续灌流。
短句来源
     Methods The isolated sympathetic ganglia,superior cervical ganglia(SCG), were made from control and morphine tolerant and dependent rats respectively.
     方法制备正常对照大鼠和Mor耐受与依赖大鼠的离体交感神经节———颈上神经节(SCG)标本,运用细胞内生物电记录技术研究Mor耐受与依赖对大鼠交感神经节快兴奋性突触传递的影响。
短句来源
     Effect of He-Ne Laser on Membrane Conductance of Postganglionic Neurons in the Isolated Rat Superior Cervical Ganglia
     He-Ne激光对大鼠离体颈上神经节后神经元膜电导的影响
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  相似匹配句对
     THE UITRASTRUCTURAL CHANGES OF DECENTRALIZED RAT SUPERIOR CERVICAL GANGLIA
     去神经的大鼠颈上交感节超微结构
短句来源
     LOCATION OF HISTAMINE IN THE SUPERIOR CERVICAL GANGLIA OF GUINEA PIG
     组胺在豚鼠颈上神经节中的分布
短句来源
     Cervical radiculopathy
     颈神经根病
短句来源
     Cervical Ganglioneuroma
     颈部神经节细胞瘤
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     Pseudocalcification of the Basal Ganglia
     基底节假性钙化
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  cervical ganglia
This was shown to potentiate the pressor effects, in some cases when applied to the superior cervical ganglia, in others to stellate ganglia, presumably by blockade of a ganglionic attenuating mechanism.
      
Stimulation of one of the lateral hypothalamus sites (TAR.I) in eight cats induced a pressor effect that was abolished by severing a nerve branch, from the superior cervical ganglia laterally, shown to innervate neck muscle vasculature.
      
We have been studying the phosphorylation of proteins of both normal and regenerating superior cervical ganglia of the rat.
      
There was also a reduction (by 30% compared to controls; p>amp;lt;0.05) in the ouabain-sensitive adenosine triphosphatase activity of the dorsal root ganglia from the diabetic rats, but their superior cervical ganglia did not show a significant fall.
      
There were also depletions of myo-inositol in the sciatic nerves and dorsal root ganglia of diabetic and galactosaemic rats, but their superior cervical ganglia contained levels of myo-inositol which were similar to those of controls.
      
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Nerve growth factor (NGF) can promote the outgrowth of neurites of the target ganglia. In order to further explore the relationship between this effect and the synthesis of RNA and DNA in the neurons, an autoradiography of 3H-uridine and 3H-thymidine was used. Superior cervical ganglia (SCG) from newborn rats were used for the material of experiments and cultivated by Maximow's double coverslip method. All cultures were divided into a group of NGF (a crude preparation of NGF was added to the medium) and...

Nerve growth factor (NGF) can promote the outgrowth of neurites of the target ganglia. In order to further explore the relationship between this effect and the synthesis of RNA and DNA in the neurons, an autoradiography of 3H-uridine and 3H-thymidine was used. Superior cervical ganglia (SCG) from newborn rats were used for the material of experiments and cultivated by Maximow's double coverslip method. All cultures were divided into a group of NGF (a crude preparation of NGF was added to the medium) and another group served as control (no NGF was added to the medium). Before tissue culture was stopped, the coverslip cultures were transferred to Petri dishes containing the labeling-medium and incubated, and then taken out to be fixated, to be made into serial sections and autoradiographic specimens, which were analysed under the light microscope. The results show that the percentage and the level of grains of neurons uabeled by 3H-uridine in a group of NGF are higher than that of control. Moreover, before the growth rate of neurites shows a peak, the level of grains of neurons labeled by 3H-uridine in a group of NGF is obviously increased. Hence, this evidence suggests that NGF can promote the synthesis of RNA in neurons of SCG, which has a direct bearing on the quick outgrowth of neurites. In the experiments with 3H-thymidine incorporation, that the NGF may promote the synthesis of DNA in some neurons of the third day SCG in vitro was also observed.

神经生长因子(NGF)可促进靶神经节神经突起生长晕的生长。本文应用~3H-尿嘧啶核苷和~3H-腺胸嘧啶核苷放射自显影术进一步探讨此作用与神经元合成RNA和DNA的关系。实验材料用新生大鼠颈上节。按Maximow双盖片法进行培养。培养物分NGF组(培养液内添加NGF粗制剂)和对照组(不用CNGF),培养终止前分别转入含放射性同位素的培养液中孵育,然后取出固定、连续切片、制作自显影标本进行观察。实验结果指出:NGF组培养物被~3H-尿嘧啶核苷标记的神经元百分率及标记颗粒水平均高于对照组,而且每当培养物神经突起生长晕生长速度出现高峰的前夕,神经元~3H-尿嘧啶核苷标记颗粒水平都是明显地增高。这说明NGF可促进颈上节神经元的RNA合成,后者与神经突起的迅速生长有一定的相互关系。在~3H-胸腺嘧啶核苷标记培养物神经元的实验中,观察到NGF可促进培养第3天的颈上节少量的神经元合成DNA。

A relatively simple method has been developed for the dissociated cell culture from neonatal rat superior cervical ganglia (SCG). Culture vessels were prepared from small culture dishes by drilling a 8.5mm hole in the bottom and cementinga 22mm glass cover slip over the outside of the hole with paraffin wax to form a shallow well inside the dish. The dissociated cells were seeded into the well precoated with reconstituted rat-tail collagen, and grown in Eagle's MEM (supplemented with 600mg% glucose) with...

A relatively simple method has been developed for the dissociated cell culture from neonatal rat superior cervical ganglia (SCG). Culture vessels were prepared from small culture dishes by drilling a 8.5mm hole in the bottom and cementinga 22mm glass cover slip over the outside of the hole with paraffin wax to form a shallow well inside the dish. The dissociated cells were seeded into the well precoated with reconstituted rat-tail collagen, and grown in Eagle's MEM (supplemented with 600mg% glucose) with 30% calf serum. Nerve growth factor (NGF, a crude extract of mouse salivary gland) was added to the medium in a proportion of 1:20. The sympathetic neurons survived and grew well in the presence of NGF. Since the cell body of neurons is large and round in shape with bright glowing appearance, the neuronal elements could be easily distinguished from the nonneuronal cells. Most sympathetic neurons show a spherical, large, euchromatic nucleus which is often situated eccentrically and contains a prominent nucleolus. The processes (neurite) from the neurons formed extensive meshworks and bundles. The tips of each fibres were expanded and flattened to form growth cones containing numerous fine filopodia, with time in culture, the neuronal cell bodies enlarged one or more than one-fold of their size, and the neurite network appeared denser. The nonneuronal cells seen were Schwann cells and fibroblasts. The capsular (satellite) cells were quite difficult to identify. The dissociated cell culture from SCG was examined by scanning electron microscopy (SEM). Three types of sympathetic neurons can be classified according to the surface morphology of their cell bodies. In type 1 neurons, their somatic surface shows microvilli, ruffles and/or small blobs. Type Ⅱ and type 111 neurons are devoid of these specialized surface structures, but the somatic surface of type Ⅱ neurons is smooth, and that of the type Ⅲ neurons is rough. Most neurons belong to type Ⅰ and type Ⅱ. Type Ⅲ neurons are less numerous in the whole cell population. Small pits can be seen on the somatic surface of some neurons at high magnification by SEM. These three types of dissociated sympathetic neurons may represent or express the different functional states or activities of neurons in culture. The possible significance of the three neuron types is discussed. Schwann cells are closely associated with neurites. Their surface is quite smooth, Fibroblasts are flattened cells with irregular contour. Their surface is smooth and shows sparse

用简化的分离细胞培养法,将新生大白鼠颈上节细胞培养在小培养皿的圆凹内。培养液含神经生长因子(nerve growth factor,NGF)粗制剂。交感节神经元获得生长,其胞体大而圆,表面折光性强,甚易与非神经元细胞区别。神经突起(neurite)自胞体发出,汇集成束或交织成网。随培养时间延长,神经元的胞体明显增大1倍或1倍以上,神经突起网亦变得更加稠密。除活体观察外,并制作了扫描电镜标本,以研究细胞的表面形态。根据分离细胞培养中交感节神经元胞体表面结构的不同,可将神经元分为3类。第1类表面有微绒毛、小泡或皱褶;第2、3类均没有这些表面结构,但第2类表面光滑;第3类则相反,表面粗糙不平。3类不同的表面结构,可能代表细胞不同的功能或活动状态。本文对此进行了一些讨论。雪旺细胞附着神经突起生长,其表面十分光滑。在分离交感神经节细胞培养中,被囊(卫星)细胞不易与游离的雪旺细胞区分。

Nerve growth factor (NGF) can promote the outgrowth of neurites of the target ganglia. In order to further explore the relationship between this effect and the synthesis of RNA and DNA in the neurons, an autoradiography of 3~H-uridine and 3~H-thymidine was used. Superior cervical ganglia (SCG) from newborn rats were cultivated by Maximow's double coverslip method. All cultures were divided to one group of cultures a crude preparation of NGF was added to the medium and another group without NGF served as...

Nerve growth factor (NGF) can promote the outgrowth of neurites of the target ganglia. In order to further explore the relationship between this effect and the synthesis of RNA and DNA in the neurons, an autoradiography of 3~H-uridine and 3~H-thymidine was used. Superior cervical ganglia (SCG) from newborn rats were cultivated by Maximow's double coverslip method. All cultures were divided to one group of cultures a crude preparation of NGF was added to the medium and another group without NGF served as control. Before tissue culture was stopped, the. covership cultures were transferred to thelabeling-medium and incubated, and then they were fixed, and cut into serial sections and subjected to autoradiographie processes. The results show that the percentage and the level of grains of neurons labeled by 3~H-uridine in the NGF group are higher than that of control. Moreover, before the growth rate of neurites reaches a peak, the level of grains of neurons labeled by 3~H-uridine in the NGF group is obviously increased. The evidence suggests that NGF can promote the synthesis of RNA in neurons of SCG, which has a direct bearing on the quick outgrowth of neurites. In the experiments with 3~H-thymidine incorporation, that the NGF may promote the synthesis of DNA in some neurons of the third day SCG in vitro was also observed.

神经生长因子(NGF)可促进靶神经节神经突起生长晕的生长。本文应用3~H-尿嘧啶核苷和3~H-胸腺嘧啶核苷放射自显影术,进一步研究该作用与神经元合成RNA和DNA的关系。用新生大鼠颈上节,按Maximow双盖片法进行培养。培养物分NGF组(培养液内添加NGF粗制剂)和对照组(不用NGF)。NGF组培养物被3~H-尿嘧啶核苷标记的神经元百分率及标记颗粒水平,均高于对照组,而且每当培养物神经突起生长晕生长速度即将出现高峰时,神经元3~H-尿嘧啶核苷标记颗粒水平都明显地增高,说明NGF可促进颈上节神经元的RNA合成,后者与神经突起的迅速生长有一定相互关系。在3~H-胸腺嘧啶核苷标记培养物神经元的实验中,观察到NGF可促进培养第3天的颈上节少量的神经元合成DNA。

 
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