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maize transgenic
相关语句
  玉米转基因
     The Selection of Some Excellent Acceptors for the Breeding of Maize Transgenic Engineering and the Studies on Their Genetic Mechanization
     玉米转基因工程育种优良受体筛选及遗传机理研究
短句来源
     Studies on the Heredity and Variation of Embryogenic Callus of Maize Transgenic Reciptients
     玉米转基因受体胚性愈伤组织的培养与遗传变异的研究
短句来源
     s:Techniques such as Agrobacteria,particulate gun and PEG inducing techniques are often adopted in maize transgenic research,While vigorous part such as callus and yong embryo typically are used as acceptance.
     玉米转基因的方法主要有农杆菌、基因枪、PEG介导等方法 ,受体主要是愈伤组织和幼胚等生活较旺盛的部位。
短句来源
  相似匹配句对
     Developments in Transgenic Maize
     玉米转基因研究进展
短句来源
     The Research and Application Transgenic Maize
     转基因玉米的研究与应用
短句来源
     and maize cross.
     与玉米杂交导入一个小麦加倍单倍体 (DH)植株中。
短句来源
     On Transgenic Plant
     转基因植物
短句来源
     TRANSGENIC PLANTS
     转基因植物
短句来源
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  maize transgenic
The efficient acceptors for maize transgenic engineering are currently insufficient in China.
      
Maize transgenic events are generated in material that has poor agronomic characteristics.
      


s:Techniques such as Agrobacteria,particulate gun and PEG inducing techniques are often adopted in maize transgenic research,While vigorous part such as callus and yong embryo typically are used as acceptance.Transgenic Bt maize and transgenic herbicide-resistant maize are successfully developed.World wide conscious to environmental pollution protection and feed chains remains obstacle to Transgenic maize application.

玉米转基因的方法主要有农杆菌、基因枪、PEG介导等方法 ,受体主要是愈伤组织和幼胚等生活较旺盛的部位。目前在玉米上的成功应用是抗玉米螟的Bt转基因玉米、抗除草剂转基因玉米等种类。转基因技术目前面临的问题是关于环境和食物链的问题。也就未来玉米的转基因发展进行了讨论。

Direct DNA delivery procedures (include biolistics method) often resulted in multiple copies of the transgenes in transformants and certain copies of them were rearranged. Integration of multiple copies of the introduced genes was the main reason of gene silencing which meant inhibition or loss of foreign gene expression in filial generations of transformants. In the present work, we compared the influences of maize Ubi-1 promoter and other promoters on copy number of transgenes in maize transgenic plants....

Direct DNA delivery procedures (include biolistics method) often resulted in multiple copies of the transgenes in transformants and certain copies of them were rearranged. Integration of multiple copies of the introduced genes was the main reason of gene silencing which meant inhibition or loss of foreign gene expression in filial generations of transformants. In the present work, we compared the influences of maize Ubi-1 promoter and other promoters on copy number of transgenes in maize transgenic plants. Immature embryos from Zea mays L. plants of sib-pollinated of A188×H99 genotype were used as initial materials. Type-Ⅰembryonic calluses derived from preculture of immature embryos were treated on N6 medium containing 0.6 mol/L sucrose for 3~5 hours and transformed via particle bombardment with PDS1000/He delivery system (Bio-Rad). Bombarded calluses were treated with hyperosmotic N6 medium for 16~20 hours continuously. Then the cultures were transferred onto normal N6 medium and incubated at 26℃ in dark for two weeks and subsequently selected on N6 medium supplemented with 2 or 5 mg/L phosphinothricin (PPT) but without casamino acid for another two weeks. The calluses after selective culture were transferred onto hormone-free MS medium containing 2 or 5 mg/L PPT but without casamino acid, and incubated at 24℃ under 16h illumination for plant regeneration. Regenerated plantlets over 2cm in height were transferred to Magenta box containing 1/2 hormone-free MS medium. Plantlets over 8cm in height were transplanted to soil. After growing for one week in greenhouse, the plants were sprayed with 250mg/L PPT solution. Fertile transgenic maize plants were regenerated and confirmed by Southern blotting and histochemical localization of β-glucuronidase (GUS) activity. Relations between promoter and copy number of transgenes in transformants were analyzed. Maize transgenic plants possessing an intact copy and another incomplete copy of β-glucuronidase gene (gus) were obtained in case gus gene under the control of maize Ubi-1 promoter (pUbi:GUS). Simultaneously the co-transformed phosphinothricin acetyltransferase gene (bar) controlled by CaMV 35S promoter in another plasmid (p35S:BAR) also existed with only one copy. When pDB1 and (pUbi:in2) were cobombarded, the regenerated transgenic maize plant exhibited with only one copy of in2 gene too. It suggested that the copy number of transgenes in maize transformants was low if the transgenes controlled by maize Ubi-1 promoter. The possible reason might be that the foreign genes were integrated site-specifically via homologous recombination between Ubi-1 promoter and its endogenous sequences in maize genome, and two cotransformed plasmids had reconstructed as one intact molecule before integrating into maize chromosome. On the contrary, if p35S:BAR was cobombarded with plasmid pAct:In1 containing rice Act-1 promoter (without maize Ubi-1 promoter), the transgenic maize plants had 4~8 copies of bar gene. These results reflected that utilization of self gene promoter could reduce the copy number of the transgenes in transgenic plants of certain species itself and avoid the occurrence of gene silencing. T2 seeds have been harvested.

通过基因枪粒子轰击和草丁膦 (PPT)选择获得可育的玉米转基因植株 ,并分析了外源基因在转化体中的拷贝数与启动子之间的关系。用玉米Ubi 1启动子驱动外源基因 ,玉米转化体中外源基因的拷贝数较低 ;可能的原因为Ubi 1启动子通过与其内部同源序列发生重组而被定点整合进玉米基因组 ,共转化的两种质粒DNA在整合至玉米染色体DNA之前已重构成为一个整体。结果显示使用某一植物自身基因的启动子可以降低外源基因在该物种转基因个体中的拷贝数 ,进而避免基因沉默现象的发生。目前已得到第二代转基因玉米种子。

The good acceptors for maize transgenic engineering are insufficient in China,especially in the southwest mountain areas.The hereditary variation regularity of maize culture capacity is not very clear.The efficiency of embryonic callus induction and the number of regenerated plantlet were used as the characters of the maize culture capacity in this research.A genetic linkage map was constructed using 88 simple sequence repeat(SSR) markers on a maize(Zea mays L.) population consisting...

The good acceptors for maize transgenic engineering are insufficient in China,especially in the southwest mountain areas.The hereditary variation regularity of maize culture capacity is not very clear.The efficiency of embryonic callus induction and the number of regenerated plantlet were used as the characters of the maize culture capacity in this research.A genetic linkage map was constructed using 88 simple sequence repeat(SSR) markers on a maize(Zea mays L.) population consisting of 202 F_2 individuals derived from the cross between the inbreds 18-599(red)(higher culture capacity) and R15 (lower culture capacity).The SSR map covers 1 636.6 cM over the ten chromosomes,with an average interval length of 18.60 cM(Fig.3) with software MAPMAKER version 3.0b.The immature embryos from the population of 202 F_(2∶3∶4) lines were evaluated in two traits which were in a normal distribution based on the investigated values(Table 1,Fig.1 and Fig.2).With the method of composite interval mapping(CIM) described in QTL Cartographer V2.0 procedure,5 quantitative trait loci(QTL) were identified for efficiency of embryonic callus induction,on chromosomes 1,3,7 and 8 respectively,accounting for phenotypic variation from 5.25% to 23.4%(Table 2);one QTL was revealed for number of regenerated plantlet on chromosome 3 and explained the phenotypic variation of 13.42%.Combining the marker assisted selection(MAS) method,the necessity and possibility will be enhanced greatly to transferring the high-culture capacity as well as to accurately location,clone and transformation of the main effect QTL of these two characters.

以18-599(红)和R15组配的F2群体,构建了含88对SSR标记的玉米遗传连锁图谱,覆盖基因组1 636.6 cM,标记间平均距离为18.6 cM。结合幼胚组织培养试验将幼胚发生胚性愈伤组织诱导率和胚性愈伤组织绿苗再分化数作为反映玉米培养过程中胚性愈伤组织诱导力和胚性愈伤组织绿苗再分化力的性状指标,以此调查玉米幼胚培养能力。利用复合区间作图法进行QTL分析,共检测到5个与胚性愈伤组织诱导率有关的QTL,位于第1、37、和8染色体上,可解释5.25%~23.4%的表型变异;检测到1个与胚性愈伤组织绿苗发生数有关的QTL,位于第3染色体上,可解释表型变异率为13.42%。

 
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