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nucleotide
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  5′核苷酸
     EVALUATION OF 5' NUCLEOTIDE PHOSPHODIESTERASE ISOZYME V FOR THE DIAGNOSIS OF PRIMARY LIVER CANCER
     探讨5′核苷酸磷酸二酯酶同工酶Ⅴ测定对原发性肝癌的诊断价值
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  “5 ' nucleotide”译为未确定词的双语例句
     DETECTION OF EARLY NEPHRIC DAMAGE FOR USING CIS DICHOLORDIAMINE PLATINUM BY URINARY 5' NUCLEOTIDE PHOSPHODIESTERASE
     尿5'-核苷酸磷酸二酯酶监测顺铂早期肾损害的研究
短句来源
     Enzymology characters of acidic phosphomonoesterase,5' phosphodiesterase and nuclease in malt root were studied. Amount of 5' nucleotide and RNA hydrolytic efficiency were used as standard. The conditions (pH,temperature,time and RNA:malt root) of RNA hydrolysis were obtained.
     研究麦芽根中酸性磷酸单酯酶 ,5’ 磷酸二酯酶及核酸水解酶的酶学特性 ,以 5’ 核苷酸和核酸水解率为指标 ,初步确定了核糖核酸 (RNA)在麦芽根作用下水解为单核苷酸的最适条件 :温度、PH值、时间及RNA与麦芽根用量比等
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  相似匹配句对
     (5).
     5、解说偏离其原有目的;
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     (5).
     5.脑GABA浓度:(1).
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     Nucleotide Sequence of 5S Ribosomal RNA from
     芹菜(Apium graveolens L.)叶细胞质5SrRNA的序列测定
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     The Process Research for 5'-Nucleotide Preparation
     5’-核苷酸制备工艺的研究
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     Study on ESI-MS of Nucleotide 5(-Thiophosphoramidates
     核苷5'-硫代磷酰氨基酸酯的电喷雾质谱研究
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  nucleotide
The MCMV-NE genome consists of 4,436 nucleotides and shares 99.5 nucleotide sequence identity with an MCMV isolate from Kansas (MCMV-KS).
      
In the 26?kDa coding region of RNA 5, there was a maximum of 1.5% nucleotide sequence differences (6 amino acid changes) within the group and 8.4% nucleotide sequence differences (17 amino acid changes) between the groups.
      
Porcine reproductive and respiratory syndrome virus (PRRSV) strains from 13 states in the United States, Guatemala and Canada were used to compare the envelope glycoprotein gene (ORF 5) nucleotide and deduced amino acid sequences.
      
Sequence comparisons revealed that XJs1 and XJs2 were most closely related to CS2-sat and CS1-sat, respectively, with 98.9% and 98.5% nucleotide sequence identity.
      
As we discuss, the available data indicate that the ILO 5( nucleotide sequence per se, not its functioning in translation initiation, is of critical importance for EAV replication.
      
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The oxidation of NADPH in the microsomal and supernatant fractions of the rat liver has been studied with the vibrating platinum electrode as well as the spectrophotometric method. An active system for the oxidation of NADPH by air, which was insensitive to cyanide, dicumarol, amytal, azide and 2,4-dinitrophenol, has been observed in the microsome fraction. It seems likely that the cytochrome oxidase system is not involved. The supernatant fraction of the liver homogenate obtained after centrifugation at 45,000×g...

The oxidation of NADPH in the microsomal and supernatant fractions of the rat liver has been studied with the vibrating platinum electrode as well as the spectrophotometric method. An active system for the oxidation of NADPH by air, which was insensitive to cyanide, dicumarol, amytal, azide and 2,4-dinitrophenol, has been observed in the microsome fraction. It seems likely that the cytochrome oxidase system is not involved. The supernatant fraction of the liver homogenate obtained after centrifugation at 45,000×g failed to catalyze the oxidation of NADPH by molecular oxygen. However, it contained a highly active reduced nicotinamide nucleotide dehydrogenase, which was found to be sensitive to dicumarol and was probably identical with the 'DT' diaphorase reported by Ernster et al. When such a supernatant fraction was added to the microsome preparation, a marked increase up to about 200% in the activity of the system for the oxidation of NADPH by air has been observed. At the same time about one half of the overall activity of this system became sensitive to dicumarol. It seems clear that the reduced nicotinamide nucleotide dehydrogenase in the supernatant fraction may be linked to certain components of the microsome fraction to produce a system capable of affecting the oxidation of NADPH by oxygen; such a system does not seem to have been hitherto reported. The physiological significance of this system for the oxidation of NADPH has been discussed.

利用自动記录白金微氧电极定氧与分光光度計测定的方法,发現大白鼠肝脏微粒体能氧化NADPH。微粒体的NADPH氧化系統活力不受氰化物、双香豆素、2,4二硝基酚、5乙,5(?)戊巴比妥酸鈉与迭氮酸鈉等所抑制。鼠肝匀浆經45,000×g离心所得的上清液不能催化NADPH被氧气所氧化。但含有对双香豆素高度敏感的还原菸酰胺核苷酸脫氫酶,其性貭与Ernster所提出的“DT黃递酶”很相似。微粒体制剂中加入上清液后NADPH氧化系統活力增大約1倍左右。此时若加入双香豆素,則NADPH氧化系統总活力被抑制大約一半。我們认为上清液中对双香豆素敏感的还原菸酰胺核苷酸脫氫酶能通过某种与微罫宓牧到M成NADPH氧化系統。这样NADPH就可以不需通过线粒体氧化。我們认为在鼠肝中NADPH的氧化途径如下: →还原性生物合成NADPH—?→通过綫粒体氧化(包括通过轉氫酶的作用) →微粒体NADPH氧化系統→O_2 →还原菸酰胺核苷酸脫氫酶→微粒体上未知系統(可能和微粒体上NADPH氧化系統部分相同) →O_2

Further studies on the stimulating and inhibiting effect of DNP on the oxidation of succinate by rat-liver mitochondria have shown that the activity of succinic dehydrogenase with 2,6-dichlorophenolindophenol as acceptor was not affected when the aerobic oxidation of succinate was almost completely inhibited by DNP. This shows that the inhibition of succinate oxidation by DNP is not due to the inhibition of the dehydrogenase. When the oxidation of succinate was inhibited by DNP, the addition of substrates of...

Further studies on the stimulating and inhibiting effect of DNP on the oxidation of succinate by rat-liver mitochondria have shown that the activity of succinic dehydrogenase with 2,6-dichlorophenolindophenol as acceptor was not affected when the aerobic oxidation of succinate was almost completely inhibited by DNP. This shows that the inhibition of succinate oxidation by DNP is not due to the inhibition of the dehydrogenase. When the oxidation of succinate was inhibited by DNP, the addition of substrates of the nicotinamide nucleotide-linked dehydrogenases could restore the inhibited respiration to a level significantly higher than the oxidation rates of these substrates themselves. The reversal of the DNP-inhibition by a-ketoglutarate required either inorganic phosphate or arsenate but was prevented by arsenite, an inhibitor of α-ketoglutaric dehydrogenase. It appears that the dehydrogenating process but not the substrate-linked phosphorylation is responsible for the effect of α-ketoglutarate. Similar effect of glutamate was not affected by high concentration of cycloserine, thus excluding the participation of L-aspartate:2-oxoglutarate aminotransferase in this action. The results presented in this communication support the view that a high-energy intermediate is required in the oxidation of succinate. It has been suggested that this high-energy compound could be formed by the reversed reaction of the succinate-linked endergonic reduction of NAD~+ in the presence of DNP.

进一步研究DNP对大白鼠肝脏綫粒体琥珀酸氧化的激活和抑制,发現当琥珀酸氧化已被DNP抑制时琥珀酸脫氫酶并沒有受到明显的抑制。DNP对琥珀酸氧化的抑制可以被α-酮戊二酸、(?)柠檬酸、柠檬酸、丙酮酸、β-羟基丁酸等解除。α-酮戊二酸的解除作用与底物水平磷酸化作用无关,但与脫氫过程有密切关系;当加入0.2mM亚砷酸鈉时,α-酮戊二酸不再能使呼吸恢复。谷氨酸解除DNP对琥珀酸氧化抑制的作用不受天門冬氨酸α-酮戊二酸轉氨酶的抑制剂环絲氨酸的影响,Amytal使呼吸恢复的作用与线粒体內源底物含量有关?疚慕Y果进一步說明琥珀酸氧化需要能量激活,我們认为某些底物脫氫生成的NADH可以通过琥珀酸氧化引起NAD~+需能还原的逆反应生成高能磷酸化合物因而激活了琥珀酸的氧化。通过这样的途径生成高能磷酸化合物可能是对DNP較不敏感的。

Brevibacterium ketoglutaricum nov. sp. 2990-6 produces a large quantity of glutamic acid in a glucose and ammonium salt medium(fermentative medium Ⅰ). But no glutamic acid is produced when it is cultivated in an inoculative medium(Ⅱ) with peptone etc. In this paper we have studied the oxidation of reduced nicotinamide nucleotides and the activities of related enzymes such as NADH_2 and NADPH_2 oxidases, nicotinamide nucleotide transhydrogenase and other dehydrogenases in cells grown in different media.Evidence...

Brevibacterium ketoglutaricum nov. sp. 2990-6 produces a large quantity of glutamic acid in a glucose and ammonium salt medium(fermentative medium Ⅰ). But no glutamic acid is produced when it is cultivated in an inoculative medium(Ⅱ) with peptone etc. In this paper we have studied the oxidation of reduced nicotinamide nucleotides and the activities of related enzymes such as NADH_2 and NADPH_2 oxidases, nicotinamide nucleotide transhydrogenase and other dehydrogenases in cells grown in different media.Evidence is presented to show that cells from medium Ⅰ contain less nicotinamide nucleotides(NAD, NADH_2, NADP, NADPH_2)than cells from medium Ⅱ. It is also shown that the activities of some NADP-linked dehydrogenases are higher in cells cultivated in medium Ⅰ than those cultivated in medium Ⅱ, although the rate of oxygen uptake for cells from Ⅰ is lower than those from Ⅱ.The mechanism of glutamic acid fermentation by this organism has been discussed.

(1)对2990-6号菌的NADH_2,NADPH_2的氧化酶系和转氢酶做了初步的观察并与2990-6号菌在发酵产生大量谷氨酸时的酶系活力的变化做了比较。(2)本工作表明NADH_2氧化酶系以及某些与NAD联结的脱氢酶,NADPH_2氧化酶系以及某些与NADP联结的脱氢酶在细胞内的分布上有着明显的区分。(3)应用比较简便的方法测定了2990-6号菌在各不同生理状态下的NAD,NADH_2,NADP,NADPH_2的含量。(4)对2990-6号菌的谷氨酸发酵机制作了简要的讨论。

 
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