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Polysaccharides extracted from radix of Chinese DC and dried Polyporus umbellate (pers) as well as a well known mitogen,phytohemagglutinin(PHA),were studied.It has been revealed that both the poly-saccharides as well as PHA stimulate the rate of DNA synthesis characterized by their increasing the incorporation of 3H-TdR and accelerating the release of thymus cells. These effects disappeared after adrenalectomy.Corticosterone concentration was found to be increased in the plasma after the administration... Polysaccharides extracted from radix of Chinese DC and dried Polyporus umbellate (pers) as well as a well known mitogen,phytohemagglutinin(PHA),were studied.It has been revealed that both the poly-saccharides as well as PHA stimulate the rate of DNA synthesis characterized by their increasing the incorporation of 3H-TdR and accelerating the release of thymus cells. These effects disappeared after adrenalectomy.Corticosterone concentration was found to be increased in the plasma after the administration of the polysaccharides These phenomena suggested that the adrenal cortex was the mediator of their actions. When they were administered to animals before an irradiation of 800 rad, the survival rate was increased by 30-70% over that of the controls. The mechanism of the effects was discussed. 从柴胡和猪苓提取的多糖,在腹腔注射后48小时,能使小鼠胸腺细胞中~sH-TdR的掺入明显增强,同时加速胸腺细胞的释放。由于一方面切除肾上腺后不再有这些表现,另一方面此多糖能使动物血浆中皮质酮含量明显增高,故认为上述作用是通过肾上腺皮质而实现的。将它们分别于照射前2和48小时予受致死剂量(800rad)全身照射的小鼠腹腔注射,可使其活存率提高30~70%,并对它们的抗辐射损伤原理进行了讨论。 The immunostimulatary effects of the polysaccharides extracted from Yin yang ho (Epimedium koreamum Nakai ) in mouse at doses of 50-200mg/kg/day for 7 days (S C) were demonstrated as follows: 1 . The number of PFC in mouse spleen was increased, and the size of plaques was enlarged. 2 . The serum level of hemo-lysin antibody from mouse immunized with SRBC was raised. 3 . The number of WBC & lymphocyte in peripheral blood and the nucleated cells in spleen were increased, and percentage of lymphocyte transformation... The immunostimulatary effects of the polysaccharides extracted from Yin yang ho (Epimedium koreamum Nakai ) in mouse at doses of 50-200mg/kg/day for 7 days (S C) were demonstrated as follows: 1 . The number of PFC in mouse spleen was increased, and the size of plaques was enlarged. 2 . The serum level of hemo-lysin antibody from mouse immunized with SRBC was raised. 3 . The number of WBC & lymphocyte in peripheral blood and the nucleated cells in spleen were increased, and percentage of lymphocyte transformation induced by PHA was enhanced. 4 . The pha-gocytic activity of peritoneal macrophage on chicken RBC was stimulated. 5 . The size of spleen was increased and that of thymus was decreased. 中药淫羊藿多糖成分50mg/kg以上剂量连续皮下给药,7天,可显著促进小鼠免疫功能。表现如下:(1)增加脾脏的PFC数,溶血空斑也显著增大。(2)提高血清溶血素水平。(3)增加外周血白细胞数、淋巴细胞及脾有核细胞数,并促进PHA刺激的淋巴细胞转化反应。(4)增强腹腔Mφ吞噬功能。(5)使脾脏重量增加,胸腺减轻。 The structural feature of six samples of agar polysaccharides extracted from four Chinese Gracilaria species, G. asiatica, G. tenuistipitata, G. blodgettii and G. eucheumoides was investigated. These agars composed mainly of charged agrarose molecules that were eluted from DEAE-Sephadex A 50 with 0.5 ad 1.0 mol/L NaCl. The yield of fractions of agar from the DNTE-Sephadex was increased to 80% or more after a treatment with 6.0 mol/L urea, with the recovery of agarose molecules having a low charge density.... The structural feature of six samples of agar polysaccharides extracted from four Chinese Gracilaria species, G. asiatica, G. tenuistipitata, G. blodgettii and G. eucheumoides was investigated. These agars composed mainly of charged agrarose molecules that were eluted from DEAE-Sephadex A 50 with 0.5 ad 1.0 mol/L NaCl. The yield of fractions of agar from the DNTE-Sephadex was increased to 80% or more after a treatment with 6.0 mol/L urea, with the recovery of agarose molecules having a low charge density. Analysis by ~(13)C-NMR spectroscopy showed 6-sulfate-L-galactose as a minor constituent in the repeat units of agarose from the main fractions of G. asiatica, G. tenuistipitata and G. blodgettii. Substitution of hydroxyl groups with methoxyl was detected by ~(13)C-NMR spectroscopy; 6-OMe-D-galactose was present as a minor sugar in agarose from G. asiatica and G. tenuistipitata, and 2-OMe-3,6-anhydro-L-galactose as a major sugar in agarose from G. eucheumoides. Differences were noted in the fractionation pattern and 6-OMe-D-galactose content of the agars from G. asiatica collected in North and South China possibly from a combination of different biological factors. Agars from gametophytes and tetrasporophytes of G. asiatica yield similar fractionation patterns on DEAE-Sephadex, but the comcentration of 3,6-anhydro-galactose in the latter fractions was somewhat higher than that of the former ones. ~(13)C-NMR spectroscopy was not sufficiently sensitive to detect the minor repeat units contributing to the charge density of agarose molecules, but it was proved to be a rapid and simple procedure to determine the types of sugars in the principal repeat units of agarose molecules that may be of taxonomic importance. 用分级法和(13)~C-NMR研究了从中国产江蓠属红藻(真江蓠、细基江蓠、芋根江蓠和凤尾菜)提取的6份琼胶多糖的结构特征。这些琼胶主要由用0.5mol/L和1.0mol/LNaCl从DEAE-Sephadex A 50层析柱洗脱下的带电荷的琼胶糖分子组成。6.0mol/L尿素级分是带低电荷密度的琼胶糖分子。各级分总得率达80%或以上。(13)~C-NMR谱图表明各种江蓠琼胶的主要级分基本上由琼胶糖结构构成,但有的级分中琼胶糖含微量6-SO_4-L-半乳糖,真江蓠和细基江蓠的琼胶糖含有6-OCH_3-D-半乳糖,而凤尾菜琼胶中则主要含有2-OCH_3-3,6-内醚-L-半乳糖。真江蓠和凤尾菜的个别级分的琼胶酶降解产物与(13)~C-NMR分析结果相一致。
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