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construct and express
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  构建和表达
     Abstract Objective: To construct and express anti-IL-2Rα genetically engineering antibody Fab and identify its biological activity.
     目的:构建和表达抗人IL-2Rα基因工程抗体Fab片段,并测定该抗体片段的生物学活性。
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     Objective To construct and express a chimeric Mth8.4 with signal peptide (MS)/ hIL12 eukaryotic expression plasmid,and to study the immunogenieity of the MS/hIL-12 chimeric genetic vaccines.
     目的构建和表达含信号肽的结核分枝杆菌8.4(MS)/人白细胞介素12(hIL-12)嵌合基因,并研究嵌合基因疫苗的免疫原性。
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     To construct and express anti human CD3 single chain Fv antibody (scFv)/human p53 tetramerization domain fusion gene, human IgG3 upper hinge was selected to be the linker between anti human CD3 scFv and human p53 tetramerization domain gene.
     为构建和表达抗人CD3单链抗体 (scFv) 人p5 3四聚功能域融合基因 ,选用人IgG3上游铰链区作为抗人CD3scFv和人p5 3四聚功能域之间连接的linker .
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     AIM To construct and express in E. coli a fusion gene of gastric cancer MG7 mimic epitope with heat shock protein 70 (hsp70).
     目的构建和表达胃癌 MG7模拟表位与人热休克蛋白70(HSP70)的融合基因.
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     Objective To construct and express recombinant plasmid containing the structural gene encoding {Mr 31 000} antigen of Trichinella spiralis muscle larvae.
     目的 构建和表达旋毛虫肌幼虫编码相对分子质量 (Mr) 3 10 0 0抗原结构基因 (TspE1)的重组质粒。
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  “construct and express”译为未确定词的双语例句
     AIM: To construct and express a eukaryotic expression plasmid containing human CD40 ligand (CD40L) in human hepatocellular carcinoma cell line HepG2 for the biological function study of CD40L on HepG2 cells.
     目的:构建含人CD40ligand(CD40L)基因的真核表达载体,并使之在人肝癌细胞HepG2中表达,研究CD40L稳定表达对HepG2细胞的影响.
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     Objective To construct and express huGM-CSF(9-127)-IL-6(29-184)fusion protein with high pu-rity and both huGM-CSF and huIL-6biologic activities.
     目的构建及表达具有huGM-CSF和huIL-6双重生物学活性的huGM-CSF(9-127)-IL-6(29-184)融合蛋白分子。
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     To construct and express Hsp70-HSV2gD fusion protein.
     构建并原核表达Hsp70-HSV2gD融合蛋白。
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     Objective To construct and express human papillomavirus type 11(HPV11) E7 gene with recombinant adenovirus vectors.
     目的构建人乳头瘤病毒11型(HPV11)E7蛋白基因腺病毒载体,并在真核细胞表达。
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     Objective To construct and express the human soluble single chain β2m-HLA-A2-BSP fusion protein expression vector.
     目的构建并表达了人可溶性单链β2m-HLA-A2-BSP(scHLA-A2)融合蛋白表达载体,为进一步制备HLA-A2四聚体及其功能研究奠定了基础。
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     IT EXPRESS
     IT新闻
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     Express
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     Objective To construct and express DNA plasmid of Trichinella spiralis.
     目的 构建和表达旋毛虫重组质粒。
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     Objective To construct and express the human angiopoietin 1 prokaryotic vector.
     目的 构建人血管生成素 - 1的原核表达载体 ,并进行表达。
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     AIM: To construct the E.
     目的 :构建融合表达ICL GFP的E .
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  construct and express
Objective: To construct and express a human-mouse chimeric antibody against human bladder cancer.
      
Identity-oriented marking serves the function of enabling individuals to both construct and express their identities to themselves and to others.
      


wo plasmids,which express chimeric recombinant antigens A(C75-C33c)and B(C33c-C75),was constructed and expressed in E. coli.The chimeric antigens were salted out and purified through ion-exchange chromatography.The immunoreactivity was tested by Western blotting,sandwich ELISA and indirect ELISA. The results indicated that the capsid and NS3 epitopes on either antigen A or B were both immunoreactive.These chimeric antigens are expected to substitute currently used mixed antigens C+C33c in HCV immunoassay....

wo plasmids,which express chimeric recombinant antigens A(C75-C33c)and B(C33c-C75),was constructed and expressed in E. coli.The chimeric antigens were salted out and purified through ion-exchange chromatography.The immunoreactivity was tested by Western blotting,sandwich ELISA and indirect ELISA. The results indicated that the capsid and NS3 epitopes on either antigen A or B were both immunoreactive.These chimeric antigens are expected to substitute currently used mixed antigens C+C33c in HCV immunoassay.

应用基因工程技术构建了两种可表达丙型肝炎病毒C蛋白和NS3蛋白双表位嵌合抗原A(C75-NS3a)和B(NS3a-C75)的质粒,并在大肠杆菌中进行了表达。采用经过盐析和离子交换层析纯化的表达产物进行Western印迹、抗C和抗NS3双抗体夹心ELISA试验,并用以检测C或NS3表位单特异性血清及系列血清,结果表明A或B抗原均同时具备C和NS3抗原的免疫反应性,而且两种抗原的性能基本相似。在HCV抗体检测中这类抗原可望替代配伍的C+C33c混合抗原。

Fusion genes in which the coding regions of the intact or partially deleted preS region of the surface antigen(HBsAg) of hepatitis B virus(HBV) was fused to a glutathione S-transferase(GST) gene were constructed and expressed in E.coli.The yield of the fusion proteins declined rapidly as the length of the HBsAg preS segment increased.Moreover,the preS region of the fusion Proteins degraded markedly,and the major cleavage sites were estimated to be around a.a.75 of the preS1 region and a.a.130 and a.a.165...

Fusion genes in which the coding regions of the intact or partially deleted preS region of the surface antigen(HBsAg) of hepatitis B virus(HBV) was fused to a glutathione S-transferase(GST) gene were constructed and expressed in E.coli.The yield of the fusion proteins declined rapidly as the length of the HBsAg preS segment increased.Moreover,the preS region of the fusion Proteins degraded markedly,and the major cleavage sites were estimated to be around a.a.75 of the preS1 region and a.a.130 and a.a.165 in the preS2 region.The research condncted with a proteinase-deficient strain revealed that the proteinases responsible for the proteolysis in the preS region existed in several E.colins and were not related to the twO major Protein degradation systems,Lon and htpR.The advantage of the GST fusion system was discussed.

我们构建了谷胱甘肽巯基转移酶(GST)和完整的或部分缺失的乙型肝炎病毒表面抗原前S区的融合基因,并在大肠杆菌中进行了表达。融合蛋白的产量随着前S区长度的增加而迅速降低,而且融合蛋白的前S区有严重的降解,主要降解位点在preS1区的a.a.75和preS2区的a.a.130和a.a.165左右。利用蛋白降解酶系缺陷型菌株进行的研究表明,这种降解酶存在于多个大肠杆菌株中而且和大肠杆菌中的两个主要的蛋白降解酶系Lon和htpR无关。具有重要生物学功能的前S区肽段(preS1a.a.1-65)因含有阻止分泌的滞留顺序而无法在哺乳动物细胞和酵母中大量表达,但滞留顺序的存在并没有影响含有这一肽段的融合蛋白在大肠杆菌中的表达和产物的纯化。GST融合表达系统产量高,纯化快速简便。用这一方法大量表达并得到的这一肽段不仅是研究乙型肝炎病毒的分子生物学的重要材料,而且可以作为新一代乙型肝炎疫苗的主要组成成分。

Site-specific mutagenesis of bacteriorhodopsin(bR)is an importanttechnique for studying the niechanism of pruton-pumping and optic-electronic fea-turee of bR molecules.The bop gene niutant with une nucleutide substitutiun of G→A at position 286 is constructed and expressed in Halubacterium HalubiuM L33(bop-).The result of mutation is identified by DNA sequencing, western blottingand absorption spectra analysis.The altered bup gene can be inherited stably in L33cells.

CONSTRUCTIONANDEXPRESSIONOFBACTERIORHODOPSINMUTANTINHALOBACTERIUMHALOBIUMWangJian(汪俭);MeiQi(梅祺);LuChunlin(卢春林)(DepartmentofBi...

 
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